Aquimarina brevivitae
Average proteome isoelectric point is 6.47
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3416 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4Q7PGE2|A0A4Q7PGE2_9FLAO Cytochrome c peroxidase OS=Aquimarina brevivitae OX=323412 GN=EV197_0783 PE=4 SV=1
MM1 pKa = 7.91 KK2 pKa = 9.85 YY3 pKa = 10.59 LKK5 pKa = 10.26 IIIGLFLSLTVIVSCKK21 pKa = 9.99 EE22 pKa = 3.81 DD23 pKa = 3.78 DD24 pKa = 3.61 SFEE27 pKa = 4.12 YY28 pKa = 10.74 LDD30 pKa = 4.51 AVEE33 pKa = 4.77 APSNITALVNITQDD47 pKa = 2.77 NTGFVTIIPTGKK59 pKa = 9.76 GAVSFDD65 pKa = 3.65 VYY67 pKa = 10.97 FGDD70 pKa = 4.16 GTLEE74 pKa = 4.14 PVNVTPGEE82 pKa = 4.22 KK83 pKa = 9.71 AEE85 pKa = 4.7 HH86 pKa = 6.53 IYY88 pKa = 11.08 EE89 pKa = 4.37 EE90 pKa = 4.49 GSYY93 pKa = 9.62 EE94 pKa = 3.73 LRR96 pKa = 11.84 IVGKK100 pKa = 9.71 SVSGKK105 pKa = 4.98 TTEE108 pKa = 3.88 AVQPLVVSFNPPQNLEE124 pKa = 3.96 VLIEE128 pKa = 3.94 NDD130 pKa = 3.25 AAVSKK135 pKa = 10.16 QVNVTATAEE144 pKa = 4.14 FAVSYY149 pKa = 10.57 DD150 pKa = 3.47 VYY152 pKa = 10.87 FGEE155 pKa = 4.7 NADD158 pKa = 4.13 DD159 pKa = 4.12 TPVSANIGDD168 pKa = 4.04 TVSYY172 pKa = 10.0 IYY174 pKa = 10.71 EE175 pKa = 3.93 NAGTYY180 pKa = 7.54 TIRR183 pKa = 11.84 VVARR187 pKa = 11.84 GGAIEE192 pKa = 4.09 TTEE195 pKa = 3.93 YY196 pKa = 10.73 TEE198 pKa = 4.21 EE199 pKa = 4.09 FTVTEE204 pKa = 3.87 ILQPVASAPAPPARR218 pKa = 11.84 STQDD222 pKa = 3.19 VISIFSAAYY231 pKa = 10.21 DD232 pKa = 3.79 DD233 pKa = 4.86 VAGTDD238 pKa = 3.87 YY239 pKa = 11.09 FPDD242 pKa = 3.45 WGQGSQGSSWSSFDD256 pKa = 4.49 LNGDD260 pKa = 3.55 EE261 pKa = 3.92 MLQYY265 pKa = 10.96 INLSYY270 pKa = 10.74 QGIQFGSPVDD280 pKa = 3.64 VSNMEE285 pKa = 4.85 FIHH288 pKa = 7.55 LDD290 pKa = 2.85 VWTSGDD296 pKa = 3.14 VSRR299 pKa = 11.84 IEE301 pKa = 3.83 TSLISVSNGEE311 pKa = 4.02 RR312 pKa = 11.84 PVWSDD317 pKa = 2.67 LTAGEE322 pKa = 4.39 WTSIDD327 pKa = 3.6 IPISAYY333 pKa = 9.27 TDD335 pKa = 3.29 QNGFTVADD343 pKa = 3.31 IHH345 pKa = 5.79 QLKK348 pKa = 10.13 FVGDD352 pKa = 3.92 PWAAGTVFIDD362 pKa = 3.47 NIYY365 pKa = 10.25 FYY367 pKa = 10.59 RR368 pKa = 11.84 APTEE372 pKa = 4.23 VINFPINFEE381 pKa = 4.79 SNNLTYY387 pKa = 9.62 TWSGFGNADD396 pKa = 4.33 FGPIPASTVNNPDD409 pKa = 3.36 PSGMNFSNTVVEE421 pKa = 4.2 IQKK424 pKa = 10.0 PAGAQVWAGASMNLSGTADD443 pKa = 4.23 FSNGTTVTMKK453 pKa = 9.95 VWSPRR458 pKa = 11.84 AGVPVLFKK466 pKa = 10.68 MEE468 pKa = 4.66 DD469 pKa = 3.25 QTSAPDD475 pKa = 3.73 GNGNPSVFVEE485 pKa = 4.57 VQATTTVANAWEE497 pKa = 4.32 EE498 pKa = 4.02 LSFDD502 pKa = 3.46 LTSYY506 pKa = 11.19 NGFSTSIPYY515 pKa = 10.69 SNVIIFPDD523 pKa = 4.56 FGNMGAGEE531 pKa = 4.55 AFYY534 pKa = 10.84 FDD536 pKa = 5.54 DD537 pKa = 5.8 IEE539 pKa = 4.26 IASLKK544 pKa = 10.6 LPLNFEE550 pKa = 4.44 VSTLTYY556 pKa = 8.48 TWAGFGDD563 pKa = 4.57 PNFGPIPAATATNPDD578 pKa = 3.24 QTGLNTSATVTEE590 pKa = 4.26 IQKK593 pKa = 8.85 PTGSQVWAGASLALDD608 pKa = 3.96 GPVDD612 pKa = 4.25 FSNGTTISLKK622 pKa = 9.73 VWSPRR627 pKa = 11.84 AGVPILFKK635 pKa = 10.6 MEE637 pKa = 4.41 DD638 pKa = 3.49 TTSPPDD644 pKa = 3.51 GNGNPTVFVEE654 pKa = 4.68 VQSSTTVANSWEE666 pKa = 4.66 EE667 pKa = 3.6 ITFDD671 pKa = 3.27 LTSFNGFSTDD681 pKa = 2.37 ISYY684 pKa = 11.51 YY685 pKa = 10.52 NVIIFPDD692 pKa = 4.59 FGNMGAGEE700 pKa = 4.45 TFYY703 pKa = 11.13 FDD705 pKa = 7.19 DD706 pKa = 5.0 IFLTNN711 pKa = 3.53
Molecular weight: 76.73 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.696
IPC2_protein 3.745
IPC_protein 3.757
Toseland 3.541
ProMoST 3.91
Dawson 3.745
Bjellqvist 3.897
Wikipedia 3.656
Rodwell 3.579
Grimsley 3.452
Solomon 3.745
Lehninger 3.694
Nozaki 3.846
DTASelect 4.075
Thurlkill 3.592
EMBOSS 3.668
Sillero 3.884
Patrickios 1.405
IPC_peptide 3.732
IPC2_peptide 3.859
IPC2.peptide.svr19 3.776
Protein with the highest isoelectric point:
>tr|A0A4Q7P1B5|A0A4Q7P1B5_9FLAO SprB-like repeat protein (Fragment) OS=Aquimarina brevivitae OX=323412 GN=EV197_2570 PE=4 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.59 RR3 pKa = 11.84 TFQPSKK9 pKa = 10.15 RR10 pKa = 11.84 KK11 pKa = 9.6 RR12 pKa = 11.84 KK13 pKa = 8.29 NKK15 pKa = 9.34 HH16 pKa = 4.03 GFRR19 pKa = 11.84 EE20 pKa = 4.05 RR21 pKa = 11.84 MASVNGRR28 pKa = 11.84 KK29 pKa = 9.21 VLARR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 10.09 GRR39 pKa = 11.84 KK40 pKa = 7.97 KK41 pKa = 10.59 LSVSSEE47 pKa = 3.56 IRR49 pKa = 11.84 HH50 pKa = 4.99 KK51 pKa = 10.53 HH52 pKa = 4.45
Molecular weight: 6.26 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.33
IPC2_protein 10.716
IPC_protein 12.266
Toseland 12.442
ProMoST 12.925
Dawson 12.442
Bjellqvist 12.427
Wikipedia 12.91
Rodwell 12.281
Grimsley 12.486
Solomon 12.939
Lehninger 12.837
Nozaki 12.442
DTASelect 12.427
Thurlkill 12.442
EMBOSS 12.939
Sillero 12.442
Patrickios 12.003
IPC_peptide 12.939
IPC2_peptide 11.915
IPC2.peptide.svr19 9.051
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3416
0
3416
1162327
27
4967
340.3
38.38
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.545 ± 0.045
0.751 ± 0.015
5.736 ± 0.034
6.59 ± 0.042
5.007 ± 0.033
6.298 ± 0.047
1.725 ± 0.021
7.891 ± 0.038
7.493 ± 0.065
9.374 ± 0.054
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.01 ± 0.022
5.892 ± 0.045
3.453 ± 0.026
3.785 ± 0.024
3.492 ± 0.033
6.323 ± 0.035
6.151 ± 0.059
6.206 ± 0.033
1.037 ± 0.013
4.242 ± 0.03
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here