Tortoise microvirus 62
Average proteome isoelectric point is 6.12
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 8 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4P8W6M5|A0A4P8W6M5_9VIRU Uncharacterized protein OS=Tortoise microvirus 62 OX=2583168 PE=4 SV=1
MM1 pKa = 7.54 KK2 pKa = 10.36 NALYY6 pKa = 10.36 CLYY9 pKa = 11.25 NMLSTRR15 pKa = 11.84 YY16 pKa = 9.69 GDD18 pKa = 3.76 VVCFPTDD25 pKa = 3.06 AYY27 pKa = 10.71 AVQRR31 pKa = 11.84 LHH33 pKa = 7.36 EE34 pKa = 4.58 DD35 pKa = 4.09 LKK37 pKa = 11.31 PEE39 pKa = 4.0 ALKK42 pKa = 10.68 EE43 pKa = 3.95 IEE45 pKa = 4.27 LCKK48 pKa = 10.1 IGYY51 pKa = 9.42 IDD53 pKa = 3.94 ISTGIAEE60 pKa = 4.16 VHH62 pKa = 5.81 PPIRR66 pKa = 11.84 IPLIVDD72 pKa = 3.58 EE73 pKa = 4.74 SVIPIDD79 pKa = 3.9 NLEE82 pKa = 4.03 KK83 pKa = 10.88
Molecular weight: 9.42 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.626
IPC2_protein 4.863
IPC_protein 4.698
Toseland 4.571
ProMoST 4.813
Dawson 4.647
Bjellqvist 4.8
Wikipedia 4.533
Rodwell 4.558
Grimsley 4.482
Solomon 4.647
Lehninger 4.596
Nozaki 4.774
DTASelect 4.914
Thurlkill 4.571
EMBOSS 4.546
Sillero 4.825
Patrickios 3.096
IPC_peptide 4.66
IPC2_peptide 4.813
IPC2.peptide.svr19 4.826
Protein with the highest isoelectric point:
>tr|A0A4V1FVZ9|A0A4V1FVZ9_9VIRU Uncharacterized protein OS=Tortoise microvirus 62 OX=2583168 PE=4 SV=1
MM1 pKa = 7.85 RR2 pKa = 11.84 FTMRR6 pKa = 11.84 SIAPSTRR13 pKa = 11.84 LVGAFRR19 pKa = 11.84 DD20 pKa = 3.99 TCSMLVGSWDD30 pKa = 3.82 CLHH33 pKa = 6.35 FTAKK37 pKa = 10.03 ALSRR41 pKa = 11.84 ILFVPRR47 pKa = 11.84 SWRR50 pKa = 11.84 CLMLTLQTVLFFQVAMLVVLISPFYY75 pKa = 9.96 FLKK78 pKa = 10.45 RR79 pKa = 11.84 LCRR82 pKa = 11.84 RR83 pKa = 11.84 LTIYY87 pKa = 10.86
Molecular weight: 10.21 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.236
IPC2_protein 9.926
IPC_protein 11.155
Toseland 11.199
ProMoST 11.579
Dawson 11.228
Bjellqvist 11.169
Wikipedia 11.637
Rodwell 11.052
Grimsley 11.272
Solomon 11.637
Lehninger 11.55
Nozaki 11.213
DTASelect 11.155
Thurlkill 11.199
EMBOSS 11.667
Sillero 11.213
Patrickios 10.847
IPC_peptide 11.637
IPC2_peptide 10.672
IPC2.peptide.svr19 9.125
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
8
0
8
1761
83
604
220.1
24.89
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.666 ± 0.885
1.533 ± 0.543
6.133 ± 0.776
4.884 ± 1.089
5.508 ± 0.709
5.224 ± 0.497
1.931 ± 0.424
4.827 ± 0.872
4.202 ± 0.872
9.143 ± 0.789
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.896 ± 0.51
4.429 ± 0.685
4.656 ± 0.591
3.805 ± 1.406
6.019 ± 0.68
10.392 ± 1.108
5.508 ± 0.6
4.997 ± 0.555
1.022 ± 0.186
5.224 ± 0.515
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here