Amino acid dipepetide frequency for Streptomyces sp. SID14478

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
21.127AlaAla: 21.127 ± 0.127
1.107AlaCys: 1.107 ± 0.022
8.474AlaAsp: 8.474 ± 0.066
8.074AlaGlu: 8.074 ± 0.074
3.707AlaPhe: 3.707 ± 0.041
12.656AlaGly: 12.656 ± 0.077
3.114AlaHis: 3.114 ± 0.038
3.402AlaIle: 3.402 ± 0.042
3.113AlaLys: 3.113 ± 0.046
14.947AlaLeu: 14.947 ± 0.1
2.614AlaMet: 2.614 ± 0.032
1.949AlaAsn: 1.949 ± 0.029
7.11AlaPro: 7.11 ± 0.053
4.286AlaGln: 4.286 ± 0.044
10.098AlaArg: 10.098 ± 0.079
6.069AlaSer: 6.069 ± 0.049
7.104AlaThr: 7.104 ± 0.053
12.085AlaVal: 12.085 ± 0.081
1.889AlaTrp: 1.889 ± 0.032
2.956AlaTyr: 2.956 ± 0.034
0.0AlaXaa: 0.0 ± 0.0
Cys
1.13CysAla: 1.13 ± 0.019
0.089CysCys: 0.089 ± 0.006
0.484CysAsp: 0.484 ± 0.013
0.355CysGlu: 0.355 ± 0.012
0.22CysPhe: 0.22 ± 0.009
0.93CysGly: 0.93 ± 0.02
0.194CysHis: 0.194 ± 0.01
0.178CysIle: 0.178 ± 0.008
0.129CysLys: 0.129 ± 0.007
0.743CysLeu: 0.743 ± 0.018
0.12CysMet: 0.12 ± 0.006
0.129CysAsn: 0.129 ± 0.006
0.464CysPro: 0.464 ± 0.014
0.149CysGln: 0.149 ± 0.007
0.566CysArg: 0.566 ± 0.015
0.433CysSer: 0.433 ± 0.012
0.539CysThr: 0.539 ± 0.018
0.691CysVal: 0.691 ± 0.017
0.122CysTrp: 0.122 ± 0.006
0.142CysTyr: 0.142 ± 0.008
0.0CysXaa: 0.0 ± 0.0
Asp
8.189AspAla: 8.189 ± 0.059
0.407AspCys: 0.407 ± 0.013
3.799AspAsp: 3.799 ± 0.043
3.943AspGlu: 3.943 ± 0.043
1.759AspPhe: 1.759 ± 0.031
6.527AspGly: 6.527 ± 0.057
1.447AspHis: 1.447 ± 0.023
1.873AspIle: 1.873 ± 0.027
1.475AspLys: 1.475 ± 0.029
6.249AspLeu: 6.249 ± 0.056
0.87AspMet: 0.87 ± 0.019
0.977AspAsn: 0.977 ± 0.021
4.376AspPro: 4.376 ± 0.045
1.505AspGln: 1.505 ± 0.025
4.653AspArg: 4.653 ± 0.05
2.636AspSer: 2.636 ± 0.037
3.327AspThr: 3.327 ± 0.036
5.066AspVal: 5.066 ± 0.044
1.052AspTrp: 1.052 ± 0.022
1.159AspTyr: 1.159 ± 0.023
0.0AspXaa: 0.0 ± 0.0
Glu
6.894GluAla: 6.894 ± 0.066
0.358GluCys: 0.358 ± 0.013
2.705GluAsp: 2.705 ± 0.039
3.257GluGlu: 3.257 ± 0.042
1.481GluPhe: 1.481 ± 0.025
4.24GluGly: 4.24 ± 0.047
1.468GluHis: 1.468 ± 0.026
2.103GluIle: 2.103 ± 0.034
1.473GluLys: 1.473 ± 0.026
6.681GluLeu: 6.681 ± 0.061
0.829GluMet: 0.829 ± 0.02
0.982GluAsn: 0.982 ± 0.019
3.066GluPro: 3.066 ± 0.041
2.277GluGln: 2.277 ± 0.031
5.324GluArg: 5.324 ± 0.059
2.519GluSer: 2.519 ± 0.03
2.663GluThr: 2.663 ± 0.035
4.228GluVal: 4.228 ± 0.047
0.728GluTrp: 0.728 ± 0.016
1.053GluTyr: 1.053 ± 0.018
0.0GluXaa: 0.0 ± 0.0
Phe
3.883PheAla: 3.883 ± 0.043
0.278PheCys: 0.278 ± 0.01
2.055PheAsp: 2.055 ± 0.034
1.387PheGlu: 1.387 ± 0.024
0.917PhePhe: 0.917 ± 0.024
3.175PheGly: 3.175 ± 0.04
0.648PheHis: 0.648 ± 0.016
0.699PheIle: 0.699 ± 0.016
0.574PheLys: 0.574 ± 0.015
2.733PheLeu: 2.733 ± 0.031
0.418PheMet: 0.418 ± 0.014
0.554PheAsn: 0.554 ± 0.016
1.431PhePro: 1.431 ± 0.026
0.693PheGln: 0.693 ± 0.017
1.815PheArg: 1.815 ± 0.029
1.501PheSer: 1.501 ± 0.026
2.159PheThr: 2.159 ± 0.033
2.375PheVal: 2.375 ± 0.03
0.44PheTrp: 0.44 ± 0.014
0.617PheTyr: 0.617 ± 0.017
0.0PheXaa: 0.0 ± 0.0
Gly
11.505GlyAla: 11.505 ± 0.072
0.811GlyCys: 0.811 ± 0.02
5.287GlyAsp: 5.287 ± 0.055
4.902GlyGlu: 4.902 ± 0.051
3.002GlyPhe: 3.002 ± 0.037
8.582GlyGly: 8.582 ± 0.072
2.311GlyHis: 2.311 ± 0.028
3.652GlyIle: 3.652 ± 0.039
2.671GlyLys: 2.671 ± 0.044
9.449GlyLeu: 9.449 ± 0.069
2.017GlyMet: 2.017 ± 0.032
1.773GlyAsn: 1.773 ± 0.029
4.899GlyPro: 4.899 ± 0.049
2.727GlyGln: 2.727 ± 0.039
7.337GlyArg: 7.337 ± 0.055
5.394GlySer: 5.394 ± 0.046
6.324GlyThr: 6.324 ± 0.053
7.663GlyVal: 7.663 ± 0.062
1.712GlyTrp: 1.712 ± 0.026
2.328GlyTyr: 2.328 ± 0.033
0.0GlyXaa: 0.0 ± 0.0
His
2.769HisAla: 2.769 ± 0.037
0.23HisCys: 0.23 ± 0.01
1.429HisAsp: 1.429 ± 0.025
1.236HisGlu: 1.236 ± 0.022
0.673HisPhe: 0.673 ± 0.016
2.473HisGly: 2.473 ± 0.033
0.71HisHis: 0.71 ± 0.016
0.643HisIle: 0.643 ± 0.014
0.418HisLys: 0.418 ± 0.012
2.507HisLeu: 2.507 ± 0.03
0.353HisMet: 0.353 ± 0.013
0.383HisAsn: 0.383 ± 0.01
1.775HisPro: 1.775 ± 0.025
0.679HisGln: 0.679 ± 0.015
2.025HisArg: 2.025 ± 0.029
1.031HisSer: 1.031 ± 0.023
1.406HisThr: 1.406 ± 0.025
1.839HisVal: 1.839 ± 0.03
0.388HisTrp: 0.388 ± 0.012
0.505HisTyr: 0.505 ± 0.015
0.0HisXaa: 0.0 ± 0.0
Ile
4.806IleAla: 4.806 ± 0.048
0.286IleCys: 0.286 ± 0.01
2.28IleAsp: 2.28 ± 0.03
1.964IleGlu: 1.964 ± 0.028
0.713IlePhe: 0.713 ± 0.017
3.731IleGly: 3.731 ± 0.043
0.603IleHis: 0.603 ± 0.015
0.842IleIle: 0.842 ± 0.02
0.799IleLys: 0.799 ± 0.019
2.421IleLeu: 2.421 ± 0.034
0.456IleMet: 0.456 ± 0.013
0.69IleAsn: 0.69 ± 0.02
1.711IlePro: 1.711 ± 0.027
0.718IleGln: 0.718 ± 0.017
2.072IleArg: 2.072 ± 0.029
1.627IleSer: 1.627 ± 0.026
2.137IleThr: 2.137 ± 0.035
2.811IleVal: 2.811 ± 0.035
0.374IleTrp: 0.374 ± 0.013
0.512IleTyr: 0.512 ± 0.014
0.0IleXaa: 0.0 ± 0.0
Lys
3.243LysAla: 3.243 ± 0.044
0.109LysCys: 0.109 ± 0.006
1.555LysAsp: 1.555 ± 0.03
1.345LysGlu: 1.345 ± 0.025
0.532LysPhe: 0.532 ± 0.016
2.065LysGly: 2.065 ± 0.038
0.47LysHis: 0.47 ± 0.014
0.888LysIle: 0.888 ± 0.02
1.114LysLys: 1.114 ± 0.026
2.127LysLeu: 2.127 ± 0.035
0.426LysMet: 0.426 ± 0.012
0.605LysAsn: 0.605 ± 0.019
1.383LysPro: 1.383 ± 0.03
0.812LysGln: 0.812 ± 0.021
1.473LysArg: 1.473 ± 0.026
1.291LysSer: 1.291 ± 0.027
1.372LysThr: 1.372 ± 0.029
2.164LysVal: 2.164 ± 0.03
0.303LysTrp: 0.303 ± 0.012
0.531LysTyr: 0.531 ± 0.014
0.0LysXaa: 0.0 ± 0.0
Leu
15.094LeuAla: 15.094 ± 0.103
0.864LeuCys: 0.864 ± 0.019
6.952LeuAsp: 6.952 ± 0.05
4.39LeuGlu: 4.39 ± 0.048
2.774LeuPhe: 2.774 ± 0.037
9.449LeuGly: 9.449 ± 0.071
2.4LeuHis: 2.4 ± 0.031
3.305LeuIle: 3.305 ± 0.037
2.253LeuLys: 2.253 ± 0.028
11.124LeuLeu: 11.124 ± 0.091
1.738LeuMet: 1.738 ± 0.026
1.741LeuAsn: 1.741 ± 0.026
6.39LeuPro: 6.39 ± 0.051
2.333LeuGln: 2.333 ± 0.031
8.337LeuArg: 8.337 ± 0.067
5.441LeuSer: 5.441 ± 0.043
7.071LeuThr: 7.071 ± 0.06
9.046LeuVal: 9.046 ± 0.072
1.343LeuTrp: 1.343 ± 0.026
1.903LeuTyr: 1.903 ± 0.033
0.0LeuXaa: 0.0 ± 0.0
Met
2.25MetAla: 2.25 ± 0.027
0.148MetCys: 0.148 ± 0.008
0.907MetAsp: 0.907 ± 0.02
0.744MetGlu: 0.744 ± 0.015
0.507MetPhe: 0.507 ± 0.014
1.376MetGly: 1.376 ± 0.029
0.38MetHis: 0.38 ± 0.012
0.68MetIle: 0.68 ± 0.018
0.475MetLys: 0.475 ± 0.013
1.735MetLeu: 1.735 ± 0.027
0.314MetMet: 0.314 ± 0.011
0.47MetAsn: 0.47 ± 0.014
1.124MetPro: 1.124 ± 0.025
0.476MetGln: 0.476 ± 0.013
1.405MetArg: 1.405 ± 0.022
1.371MetSer: 1.371 ± 0.023
1.576MetThr: 1.576 ± 0.022
1.306MetVal: 1.306 ± 0.023
0.229MetTrp: 0.229 ± 0.01
0.339MetTyr: 0.339 ± 0.012
0.0MetXaa: 0.0 ± 0.0
Asn
2.18AsnAla: 2.18 ± 0.034
0.159AsnCys: 0.159 ± 0.008
0.978AsnAsp: 0.978 ± 0.022
0.805AsnGlu: 0.805 ± 0.018
0.514AsnPhe: 0.514 ± 0.014
1.912AsnGly: 1.912 ± 0.031
0.406AsnHis: 0.406 ± 0.015
0.668AsnIle: 0.668 ± 0.017
0.474AsnLys: 0.474 ± 0.015
1.683AsnLeu: 1.683 ± 0.025
0.321AsnMet: 0.321 ± 0.01
0.439AsnAsn: 0.439 ± 0.014
1.33AsnPro: 1.33 ± 0.021
0.517AsnGln: 0.517 ± 0.016
1.177AsnArg: 1.177 ± 0.02
0.977AsnSer: 0.977 ± 0.022
1.142AsnThr: 1.142 ± 0.026
1.403AsnVal: 1.403 ± 0.024
0.303AsnTrp: 0.303 ± 0.01
0.425AsnTyr: 0.425 ± 0.014
0.0AsnXaa: 0.0 ± 0.0
Pro
8.257ProAla: 8.257 ± 0.058
0.319ProCys: 0.319 ± 0.012
4.445ProAsp: 4.445 ± 0.05
3.879ProGlu: 3.879 ± 0.04
1.541ProPhe: 1.541 ± 0.025
6.18ProGly: 6.18 ± 0.053
1.47ProHis: 1.47 ± 0.023
1.243ProIle: 1.243 ± 0.024
1.287ProLys: 1.287 ± 0.025
5.439ProLeu: 5.439 ± 0.051
1.028ProMet: 1.028 ± 0.021
0.878ProAsn: 0.878 ± 0.018
3.073ProPro: 3.073 ± 0.047
1.939ProGln: 1.939 ± 0.035
3.722ProArg: 3.722 ± 0.041
3.055ProSer: 3.055 ± 0.038
3.151ProThr: 3.151 ± 0.037
5.214ProVal: 5.214 ± 0.042
0.873ProTrp: 0.873 ± 0.019
1.403ProTyr: 1.403 ± 0.026
0.0ProXaa: 0.0 ± 0.0
Gln
3.839GlnAla: 3.839 ± 0.04
0.174GlnCys: 0.174 ± 0.009
1.577GlnAsp: 1.577 ± 0.025
1.606GlnGlu: 1.606 ± 0.026
0.732GlnPhe: 0.732 ± 0.02
2.538GlnGly: 2.538 ± 0.031
0.697GlnHis: 0.697 ± 0.017
1.11GlnIle: 1.11 ± 0.024
0.735GlnLys: 0.735 ± 0.019
3.179GlnLeu: 3.179 ± 0.034
0.532GlnMet: 0.532 ± 0.016
0.533GlnAsn: 0.533 ± 0.016
1.639GlnPro: 1.639 ± 0.029
1.385GlnGln: 1.385 ± 0.032
2.371GlnArg: 2.371 ± 0.032
1.297GlnSer: 1.297 ± 0.022
1.384GlnThr: 1.384 ± 0.025
2.485GlnVal: 2.485 ± 0.033
0.523GlnTrp: 0.523 ± 0.015
0.665GlnTyr: 0.665 ± 0.016
0.0GlnXaa: 0.0 ± 0.0
Arg
9.615ArgAla: 9.615 ± 0.08
0.569ArgCys: 0.569 ± 0.016
4.236ArgAsp: 4.236 ± 0.048
4.459ArgGlu: 4.459 ± 0.046
2.252ArgPhe: 2.252 ± 0.033
5.777ArgGly: 5.777 ± 0.054
2.063ArgHis: 2.063 ± 0.029
3.251ArgIle: 3.251 ± 0.035
1.681ArgLys: 1.681 ± 0.027
8.356ArgLeu: 8.356 ± 0.082
1.654ArgMet: 1.654 ± 0.023
1.325ArgAsn: 1.325 ± 0.027
4.532ArgPro: 4.532 ± 0.05
2.186ArgGln: 2.186 ± 0.032
7.119ArgArg: 7.119 ± 0.069
3.848ArgSer: 3.848 ± 0.04
5.445ArgThr: 5.445 ± 0.045
5.656ArgVal: 5.656 ± 0.055
1.299ArgTrp: 1.299 ± 0.023
1.7ArgTyr: 1.7 ± 0.025
0.0ArgXaa: 0.0 ± 0.0
Ser
6.878SerAla: 6.878 ± 0.057
0.383SerCys: 0.383 ± 0.014
2.782SerAsp: 2.782 ± 0.032
2.334SerGlu: 2.334 ± 0.03
1.579SerPhe: 1.579 ± 0.024
5.992SerGly: 5.992 ± 0.058
1.041SerHis: 1.041 ± 0.02
1.39SerIle: 1.39 ± 0.025
1.127SerLys: 1.127 ± 0.025
5.034SerLeu: 5.034 ± 0.048
1.098SerMet: 1.098 ± 0.02
0.887SerAsn: 0.887 ± 0.018
3.067SerPro: 3.067 ± 0.036
1.352SerGln: 1.352 ± 0.022
3.519SerArg: 3.519 ± 0.039
2.898SerSer: 2.898 ± 0.038
3.096SerThr: 3.096 ± 0.035
4.356SerVal: 4.356 ± 0.041
0.951SerTrp: 0.951 ± 0.022
1.319SerTyr: 1.319 ± 0.023
0.0SerXaa: 0.0 ± 0.0
Thr
8.671ThrAla: 8.671 ± 0.076
0.45ThrCys: 0.45 ± 0.015
3.758ThrAsp: 3.758 ± 0.041
3.058ThrGlu: 3.058 ± 0.036
1.727ThrPhe: 1.727 ± 0.027
6.618ThrGly: 6.618 ± 0.055
1.257ThrHis: 1.257 ± 0.021
1.702ThrIle: 1.702 ± 0.031
1.314ThrLys: 1.314 ± 0.029
5.96ThrLeu: 5.96 ± 0.048
0.965ThrMet: 0.965 ± 0.022
1.035ThrAsn: 1.035 ± 0.022
3.988ThrPro: 3.988 ± 0.041
1.526ThrGln: 1.526 ± 0.023
3.86ThrArg: 3.86 ± 0.044
3.365ThrSer: 3.365 ± 0.043
3.902ThrThr: 3.902 ± 0.046
6.083ThrVal: 6.083 ± 0.06
0.954ThrTrp: 0.954 ± 0.023
1.56ThrTyr: 1.56 ± 0.029
0.0ThrXaa: 0.0 ± 0.0
Val
10.854ValAla: 10.854 ± 0.074
0.745ValCys: 0.745 ± 0.016
5.149ValAsp: 5.149 ± 0.052
4.591ValGlu: 4.591 ± 0.043
2.497ValPhe: 2.497 ± 0.034
6.669ValGly: 6.669 ± 0.055
2.029ValHis: 2.029 ± 0.033
2.873ValIle: 2.873 ± 0.039
1.845ValLys: 1.845 ± 0.028
9.649ValLeu: 9.649 ± 0.071
1.475ValMet: 1.475 ± 0.021
1.651ValAsn: 1.651 ± 0.028
5.175ValPro: 5.175 ± 0.047
2.171ValGln: 2.171 ± 0.031
7.084ValArg: 7.084 ± 0.064
4.382ValSer: 4.382 ± 0.05
5.751ValThr: 5.751 ± 0.051
8.078ValVal: 8.078 ± 0.08
1.15ValTrp: 1.15 ± 0.022
1.586ValTyr: 1.586 ± 0.023
0.0ValXaa: 0.0 ± 0.0
Trp
1.696TrpAla: 1.696 ± 0.023
0.156TrpCys: 0.156 ± 0.008
0.864TrpAsp: 0.864 ± 0.018
0.685TrpGlu: 0.685 ± 0.017
0.536TrpPhe: 0.536 ± 0.016
1.11TrpGly: 1.11 ± 0.024
0.388TrpHis: 0.388 ± 0.012
0.583TrpIle: 0.583 ± 0.017
0.393TrpLys: 0.393 ± 0.014
1.796TrpLeu: 1.796 ± 0.03
0.314TrpMet: 0.314 ± 0.011
0.405TrpAsn: 0.405 ± 0.012
0.771TrpPro: 0.771 ± 0.016
0.655TrpGln: 0.655 ± 0.014
1.301TrpArg: 1.301 ± 0.028
0.961TrpSer: 0.961 ± 0.02
1.011TrpThr: 1.011 ± 0.021
1.013TrpVal: 1.013 ± 0.021
0.338TrpTrp: 0.338 ± 0.013
0.38TrpTyr: 0.38 ± 0.012
0.0TrpXaa: 0.0 ± 0.0
Tyr
3.002TyrAla: 3.002 ± 0.035
0.165TyrCys: 0.165 ± 0.008
1.571TyrAsp: 1.571 ± 0.032
1.316TyrGlu: 1.316 ± 0.023
0.69TyrPhe: 0.69 ± 0.017
2.344TyrGly: 2.344 ± 0.03
0.377TyrHis: 0.377 ± 0.014
0.469TyrIle: 0.469 ± 0.014
0.458TyrLys: 0.458 ± 0.016
2.139TyrLeu: 2.139 ± 0.028
0.272TyrMet: 0.272 ± 0.01
0.408TyrAsn: 0.408 ± 0.013
1.092TyrPro: 1.092 ± 0.024
0.615TyrGln: 0.615 ± 0.014
1.763TyrArg: 1.763 ± 0.03
0.986TyrSer: 0.986 ± 0.019
1.193TyrThr: 1.193 ± 0.023
1.843TyrVal: 1.843 ± 0.03
0.382TyrTrp: 0.382 ± 0.013
0.494TyrTyr: 0.494 ± 0.015
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 8919 proteins (2583583 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski