Methylocystis sp. (strain SC2)

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales; Methylocystaceae; Methylocystis; unclassified Methylocystis

Average proteome isoelectric point is 6.89

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4040 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|I4EAP4|I4EAP4_METSZ Polyphosphate kinase OS=Methylocystis sp. (strain SC2) OX=187303 GN=SC2p1_01340 PE=3 SV=1
MM1 pKa = 7.64TEE3 pKa = 4.22MDD5 pKa = 4.6MDD7 pKa = 4.73DD8 pKa = 4.71CLEE11 pKa = 4.1GMNDD15 pKa = 3.55DD16 pKa = 3.76RR17 pKa = 11.84AGALDD22 pKa = 4.71CAALTCGLTQTILPPHH38 pKa = 7.05EE39 pKa = 4.14IVITSIIATCASPLTPRR56 pKa = 11.84DD57 pKa = 3.39DD58 pKa = 4.08HH59 pKa = 6.37EE60 pKa = 4.93RR61 pKa = 11.84CGLSGPPDD69 pKa = 4.12LRR71 pKa = 11.84PPIAA75 pKa = 5.1

Molecular weight: 7.96 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|J7QS73|J7QS73_METSZ Cation diffusion facilitator family transporter OS=Methylocystis sp. (strain SC2) OX=187303 GN=BN69_2932 PE=3 SV=1
MM1 pKa = 7.31SLLRR5 pKa = 11.84RR6 pKa = 11.84GRR8 pKa = 11.84RR9 pKa = 11.84RR10 pKa = 11.84RR11 pKa = 11.84QTRR14 pKa = 11.84RR15 pKa = 11.84ARR17 pKa = 11.84EE18 pKa = 3.86LGASPFRR25 pKa = 11.84QRR27 pKa = 11.84RR28 pKa = 11.84RR29 pKa = 11.84TVKK32 pKa = 9.71RR33 pKa = 11.84TFQPSKK39 pKa = 8.96IVRR42 pKa = 11.84KK43 pKa = 9.24RR44 pKa = 11.84RR45 pKa = 11.84HH46 pKa = 4.55GFRR49 pKa = 11.84ARR51 pKa = 11.84MATVGGRR58 pKa = 11.84NVIAARR64 pKa = 11.84RR65 pKa = 11.84ARR67 pKa = 11.84GRR69 pKa = 11.84KK70 pKa = 9.03RR71 pKa = 11.84LSAA74 pKa = 4.03

Molecular weight: 8.84 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4040

0

4040

1212217

34

3657

300.1

32.65

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.392 ± 0.062

0.971 ± 0.014

5.66 ± 0.028

5.906 ± 0.037

3.95 ± 0.025

8.102 ± 0.042

2.014 ± 0.016

4.932 ± 0.029

3.455 ± 0.028

9.986 ± 0.042

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.187 ± 0.017

2.531 ± 0.024

5.29 ± 0.035

2.955 ± 0.02

7.853 ± 0.047

5.55 ± 0.027

4.72 ± 0.031

6.989 ± 0.036

1.328 ± 0.017

2.229 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski