Heterocapsa circularisquama RNA virus 01 (strain 34) (HcRNAV01)

Taxonomy: Viruses; Riboviria; Orthornavirae; Pisuviricota; Pisoniviricetes; Sobelivirales; Alvernaviridae; Dinornavirus; Heterocapsa circularisquama RNA virus 01

Average proteome isoelectric point is 7.63

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q3C207|Q3C207_HCRNA RNA-directed RNA polymerase OS=Heterocapsa circularisquama RNA virus 01 (strain 34) OX=1289474 GN=HcRNAV34 ORF-1 PE=4 SV=1
MM1 pKa = 7.28VFLTAAISTSLWATGTTGAVGATTYY26 pKa = 10.67FGVKK30 pKa = 8.58FRR32 pKa = 11.84RR33 pKa = 11.84RR34 pKa = 11.84VRR36 pKa = 11.84AWLHH40 pKa = 4.28YY41 pKa = 9.38RR42 pKa = 11.84QLNLRR47 pKa = 11.84VGHH50 pKa = 5.57FTSKK54 pKa = 10.74GKK56 pKa = 10.33LFYY59 pKa = 10.85RR60 pKa = 11.84EE61 pKa = 4.4DD62 pKa = 3.95ADD64 pKa = 3.92GPLYY68 pKa = 10.43QAVLKK73 pKa = 10.23RR74 pKa = 11.84SKK76 pKa = 10.66GINPRR81 pKa = 11.84GEE83 pKa = 4.88LIDD86 pKa = 4.09PEE88 pKa = 4.15YY89 pKa = 10.89DD90 pKa = 3.49YY91 pKa = 11.9TEE93 pKa = 4.89LSTSLMSFNEE103 pKa = 3.97SSLVGSTPVRR113 pKa = 11.84RR114 pKa = 11.84IPRR117 pKa = 11.84SLAPSHH123 pKa = 6.34LVVLFSDD130 pKa = 4.1DD131 pKa = 3.46QVSGVGFRR139 pKa = 11.84MGEE142 pKa = 3.81NLITARR148 pKa = 11.84HH149 pKa = 5.34VLDD152 pKa = 3.78LTHH155 pKa = 6.57NLWVQGPRR163 pKa = 11.84GRR165 pKa = 11.84IRR167 pKa = 11.84VEE169 pKa = 4.14EE170 pKa = 4.29PPVTPPVSDD179 pKa = 3.9YY180 pKa = 10.48EE181 pKa = 4.26YY182 pKa = 10.05TGADD186 pKa = 2.6ICYY189 pKa = 9.87IPLTPGQWADD199 pKa = 3.77LGVKK203 pKa = 9.53SMKK206 pKa = 10.25KK207 pKa = 9.76NAVSLSAAGLIKK219 pKa = 10.64LYY221 pKa = 10.87GADD224 pKa = 3.6EE225 pKa = 5.05DD226 pKa = 4.52GLYY229 pKa = 10.86EE230 pKa = 4.75SIGPLKK236 pKa = 10.46VDD238 pKa = 3.81PSLTKK243 pKa = 10.59GKK245 pKa = 10.74GLISYY250 pKa = 7.61VASTTPGFSGSPVLLRR266 pKa = 11.84NAGGGEE272 pKa = 4.48SVVGMHH278 pKa = 6.19VCGDD282 pKa = 3.93FKK284 pKa = 11.59GNGANHH290 pKa = 6.41GVGASSINLILKK302 pKa = 7.82GTGAVPDD309 pKa = 4.46ALTVSDD315 pKa = 4.89FLGPSKK321 pKa = 10.81LEE323 pKa = 3.62SAMYY327 pKa = 10.1YY328 pKa = 10.47DD329 pKa = 3.78KK330 pKa = 11.52QEE332 pKa = 4.51TYY334 pKa = 9.89WDD336 pKa = 3.25WVFDD340 pKa = 3.85QEE342 pKa = 5.26CEE344 pKa = 4.03RR345 pKa = 11.84EE346 pKa = 4.0EE347 pKa = 4.0AARR350 pKa = 11.84EE351 pKa = 4.05AEE353 pKa = 4.12EE354 pKa = 5.33AEE356 pKa = 4.19IEE358 pKa = 4.22HH359 pKa = 7.04LRR361 pKa = 11.84DD362 pKa = 3.3LYY364 pKa = 11.4YY365 pKa = 11.26GEE367 pKa = 4.37TTLALAPVAPPVPSFNGPGARR388 pKa = 11.84ALRR391 pKa = 11.84SYY393 pKa = 11.21NEE395 pKa = 3.92SALNVPARR403 pKa = 11.84LGEE406 pKa = 3.89LSPRR410 pKa = 11.84VGPVEE415 pKa = 4.35SSTHH419 pKa = 5.53LPRR422 pKa = 11.84FLMGASTEE430 pKa = 4.43RR431 pKa = 11.84FWSRR435 pKa = 11.84SINDD439 pKa = 3.81TPPAPLQRR447 pKa = 11.84EE448 pKa = 4.43SPTLKK453 pKa = 9.72ATPEE457 pKa = 4.15LVVSMFTKK465 pKa = 10.23IAEE468 pKa = 4.49GDD470 pKa = 3.45HH471 pKa = 6.55KK472 pKa = 11.36AFLLAIDD479 pKa = 4.28MSYY482 pKa = 11.73DD483 pKa = 3.9DD484 pKa = 5.95LLNCTKK490 pKa = 10.92DD491 pKa = 3.32EE492 pKa = 4.16VMDD495 pKa = 4.37PFSDD499 pKa = 3.53FRR501 pKa = 11.84QYY503 pKa = 11.36LEE505 pKa = 3.98HH506 pKa = 6.33TRR508 pKa = 11.84KK509 pKa = 9.88LMKK512 pKa = 10.65KK513 pKa = 9.12NDD515 pKa = 3.74GGRR518 pKa = 11.84EE519 pKa = 3.87LSDD522 pKa = 3.07QSGRR526 pKa = 11.84TFFRR530 pKa = 11.84EE531 pKa = 3.94THH533 pKa = 5.92RR534 pKa = 11.84TTQSGGGKK542 pKa = 9.59KK543 pKa = 8.32RR544 pKa = 11.84TAPVWLNEE552 pKa = 3.78EE553 pKa = 3.98SKK555 pKa = 11.34AFLRR559 pKa = 11.84GLGVEE564 pKa = 4.67GEE566 pKa = 4.47YY567 pKa = 10.71CRR569 pKa = 11.84PPNDD573 pKa = 3.31EE574 pKa = 3.91ASIIRR579 pKa = 11.84SMEE582 pKa = 3.69NQAARR587 pKa = 11.84QLGSRR592 pKa = 11.84AWPSTGAMEE601 pKa = 4.6PFLVQYY607 pKa = 10.72LGDD610 pKa = 3.99AAVSPPTFSPGRR622 pKa = 11.84QGFDD626 pKa = 2.83QLYY629 pKa = 10.2RR630 pKa = 11.84SFDD633 pKa = 3.6DD634 pKa = 5.67SSAGWTRR641 pKa = 11.84RR642 pKa = 11.84YY643 pKa = 10.52RR644 pKa = 11.84NLTKK648 pKa = 10.2KK649 pKa = 10.42SYY651 pKa = 11.09VSGPHH656 pKa = 6.29CGEE659 pKa = 3.83LTRR662 pKa = 11.84IAVARR667 pKa = 11.84ILLRR671 pKa = 11.84CTMWHH676 pKa = 7.51RR677 pKa = 11.84IPSMTPEE684 pKa = 3.81EE685 pKa = 4.41MVWLGLRR692 pKa = 11.84DD693 pKa = 3.72PTDD696 pKa = 3.17VFVKK700 pKa = 10.57DD701 pKa = 4.17EE702 pKa = 4.03PHH704 pKa = 6.1TADD707 pKa = 3.35KK708 pKa = 10.86ARR710 pKa = 11.84RR711 pKa = 11.84EE712 pKa = 3.91MWRR715 pKa = 11.84LIWNVSLVDD724 pKa = 4.8SICQAYY730 pKa = 9.65FNRR733 pKa = 11.84EE734 pKa = 3.51LNLQQNRR741 pKa = 11.84DD742 pKa = 3.79YY743 pKa = 11.29QGGHH747 pKa = 6.53PVPHH751 pKa = 5.99TCGMGHH757 pKa = 6.82HH758 pKa = 7.44DD759 pKa = 4.27EE760 pKa = 4.96GIKK763 pKa = 10.55RR764 pKa = 11.84LGEE767 pKa = 4.31AIEE770 pKa = 4.04AAFPDD775 pKa = 5.22GIVCSSDD782 pKa = 2.87ASGWDD787 pKa = 3.24MSVSRR792 pKa = 11.84DD793 pKa = 3.27GLIFDD798 pKa = 4.06GLVRR802 pKa = 11.84AIRR805 pKa = 11.84TQDD808 pKa = 3.33PSATAYY814 pKa = 10.58HH815 pKa = 6.58NIGTSVCILLDD826 pKa = 3.43KK827 pKa = 10.45FVQSAHH833 pKa = 6.22MICTGTSLWSVDD845 pKa = 3.44VYY847 pKa = 11.4GITASGLPDD856 pKa = 3.25TTTQNSFVRR865 pKa = 11.84GMGAKK870 pKa = 10.06LAGCFKK876 pKa = 10.97ALTAGDD882 pKa = 5.61DD883 pKa = 3.92LLCDD887 pKa = 3.33NRR889 pKa = 11.84LRR891 pKa = 11.84LPVLTEE897 pKa = 3.97HH898 pKa = 6.15GTITKK903 pKa = 10.69GDD905 pKa = 3.49VTTANWRR912 pKa = 11.84KK913 pKa = 8.04GQPVGFTSHH922 pKa = 6.3SLVRR926 pKa = 11.84GPDD929 pKa = 4.08GQWSAHH935 pKa = 5.74FEE937 pKa = 4.23NEE939 pKa = 3.79SKK941 pKa = 10.84ALHH944 pKa = 6.33RR945 pKa = 11.84LLLSGKK951 pKa = 8.0TPLPEE956 pKa = 4.1QIGGVAFAEE965 pKa = 4.44RR966 pKa = 11.84KK967 pKa = 10.19DD968 pKa = 3.7EE969 pKa = 4.32AQLGRR974 pKa = 11.84LRR976 pKa = 11.84AVCDD980 pKa = 3.5SRR982 pKa = 11.84GWPVPPQSEE991 pKa = 4.06WAEE994 pKa = 4.2DD995 pKa = 3.56FDD997 pKa = 5.27LCC999 pKa = 5.79

Molecular weight: 110.08 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q3C207|Q3C207_HCRNA RNA-directed RNA polymerase OS=Heterocapsa circularisquama RNA virus 01 (strain 34) OX=1289474 GN=HcRNAV34 ORF-1 PE=4 SV=1
MM1 pKa = 7.25TRR3 pKa = 11.84PLALTNGGNTNGGNNGGSRR22 pKa = 11.84RR23 pKa = 11.84RR24 pKa = 11.84PPRR27 pKa = 11.84QRR29 pKa = 11.84RR30 pKa = 11.84QGRR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84NRR37 pKa = 11.84RR38 pKa = 11.84RR39 pKa = 11.84GGGGGGPRR47 pKa = 11.84NNAAMVLAQGAGSVPGMPFGSWPSRR72 pKa = 11.84STMRR76 pKa = 11.84AWDD79 pKa = 3.7AFHH82 pKa = 7.53PEE84 pKa = 4.35HH85 pKa = 6.81LPLPRR90 pKa = 11.84SVGPYY95 pKa = 9.14CVVRR99 pKa = 11.84TSSLITSSDD108 pKa = 2.93KK109 pKa = 11.5VMLFAPMVGSAGCWLTACAMGSRR132 pKa = 11.84TEE134 pKa = 4.05GGAINGLDD142 pKa = 3.45NTNVYY147 pKa = 8.22TVPFPGIATTGSSITVVPAALSVQVMNPNPLMSTTGIFGGTVSHH191 pKa = 6.25TQLNLAGRR199 pKa = 11.84TEE201 pKa = 3.96TWNDD205 pKa = 2.75FSMEE209 pKa = 4.36VISFMRR215 pKa = 11.84PRR217 pKa = 11.84LMSAGKK223 pKa = 9.4LALRR227 pKa = 11.84GVQGDD232 pKa = 4.24SYY234 pKa = 9.57PLNMSALSNFNCLSDD249 pKa = 3.7VAEE252 pKa = 5.38GKK254 pKa = 10.69LSWTDD259 pKa = 3.28SSGHH263 pKa = 5.54YY264 pKa = 9.38PAGLAPLVFVNEE276 pKa = 4.63AKK278 pKa = 8.88QTMNYY283 pKa = 9.07LVSVEE288 pKa = 3.55WRR290 pKa = 11.84VRR292 pKa = 11.84FDD294 pKa = 2.93IGNPAVAAQRR304 pKa = 11.84HH305 pKa = 5.57HH306 pKa = 7.3GITPEE311 pKa = 4.07WKK313 pKa = 8.29WDD315 pKa = 3.79DD316 pKa = 3.88MIKK319 pKa = 9.12TAIARR324 pKa = 11.84GHH326 pKa = 6.83GIMDD330 pKa = 3.46IAEE333 pKa = 4.04RR334 pKa = 11.84VANAGSFVANAAIAARR350 pKa = 11.84RR351 pKa = 11.84AMPALMAAA359 pKa = 4.3

Molecular weight: 38.24 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

1358

359

999

679.0

74.16

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.542 ± 1.373

1.473 ± 0.189

5.228 ± 1.143

5.007 ± 1.32

3.535 ± 0.248

9.794 ± 0.859

2.356 ± 0.215

3.535 ± 0.046

3.314 ± 0.867

8.763 ± 1.097

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.872 ± 1.131

3.829 ± 1.361

6.186 ± 0.264

2.651 ± 0.223

7.364 ± 0.524

8.027 ± 0.027

6.406 ± 0.0

6.554 ± 0.216

2.062 ± 0.088

2.504 ± 0.587
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski