Amino acid dipepetide frequency for Clostridium sp. CAG:58

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	10.323AlaAla: 10.323 ± 0.163	1.268AlaCys: 1.268 ± 0.042	4.734AlaAsp: 4.734 ± 0.08	6.402AlaGlu: 6.402 ± 0.108	3.405AlaPhe: 3.405 ± 0.077	8.002AlaGly: 8.002 ± 0.114	1.273AlaHis: 1.273 ± 0.046	4.545AlaIle: 4.545 ± 0.075	3.823AlaLys: 3.823 ± 0.071	7.895AlaLeu: 7.895 ± 0.12	2.993AlaMet: 2.993 ± 0.066	2.062AlaAsn: 2.062 ± 0.054	2.67AlaPro: 2.67 ± 0.072	2.117AlaGln: 2.117 ± 0.054	4.213AlaArg: 4.213 ± 0.083	4.503AlaSer: 4.503 ± 0.073	2.72AlaThr: 2.72 ± 0.06	7.833AlaVal: 7.833 ± 0.115	0.763AlaTrp: 0.763 ± 0.028	2.679AlaTyr: 2.679 ± 0.066	0.009AlaXaa: 0.009 ± 0.003
Cys	1.044CysAla: 1.044 ± 0.037	0.322CysCys: 0.322 ± 0.022	0.761CysAsp: 0.761 ± 0.03	0.842CysGlu: 0.842 ± 0.029	0.633CysPhe: 0.633 ± 0.03	1.737CysGly: 1.737 ± 0.048	0.319CysHis: 0.319 ± 0.02	0.923CysIle: 0.923 ± 0.035	0.587CysLys: 0.587 ± 0.028	1.267CysLeu: 1.267 ± 0.042	0.45CysMet: 0.45 ± 0.023	0.441CysAsn: 0.441 ± 0.023	0.758CysPro: 0.758 ± 0.036	0.451CysGln: 0.451 ± 0.025	0.977CysArg: 0.977 ± 0.04	0.868CysSer: 0.868 ± 0.033	0.785CysThr: 0.785 ± 0.036	0.98CysVal: 0.98 ± 0.033	0.12CysTrp: 0.12 ± 0.011	0.5CysTyr: 0.5 ± 0.024	0.0CysXaa: 0.0 ± 0.0
Asp	4.249AspAla: 4.249 ± 0.079	0.789AspCys: 0.789 ± 0.03	2.485AspAsp: 2.485 ± 0.07	3.927AspGlu: 3.927 ± 0.082	2.443AspPhe: 2.443 ± 0.057	5.195AspGly: 5.195 ± 0.095	1.22AspHis: 1.22 ± 0.043	3.477AspIle: 3.477 ± 0.075	2.488AspLys: 2.488 ± 0.067	5.015AspLeu: 5.015 ± 0.085	1.966AspMet: 1.966 ± 0.055	1.446AspAsn: 1.446 ± 0.044	2.545AspPro: 2.545 ± 0.057	1.808AspGln: 1.808 ± 0.046	3.167AspArg: 3.167 ± 0.075	2.897AspSer: 2.897 ± 0.066	2.871AspThr: 2.871 ± 0.071	3.772AspVal: 3.772 ± 0.071	0.566AspTrp: 0.566 ± 0.028	2.192AspTyr: 2.192 ± 0.054	0.0AspXaa: 0.0 ± 0.0
Glu	6.729GluAla: 6.729 ± 0.114	0.744GluCys: 0.744 ± 0.03	4.24GluAsp: 4.24 ± 0.081	7.583GluGlu: 7.583 ± 0.131	2.579GluPhe: 2.579 ± 0.059	4.824GluGly: 4.824 ± 0.083	1.262GluHis: 1.262 ± 0.043	5.171GluIle: 5.171 ± 0.088	6.421GluLys: 6.421 ± 0.101	7.039GluLeu: 7.039 ± 0.113	2.425GluMet: 2.425 ± 0.043	3.809GluAsn: 3.809 ± 0.074	2.365GluPro: 2.365 ± 0.06	2.352GluGln: 2.352 ± 0.059	4.132GluArg: 4.132 ± 0.085	3.3GluSer: 3.3 ± 0.067	4.38GluThr: 4.38 ± 0.08	4.098GluVal: 4.098 ± 0.064	0.621GluTrp: 0.621 ± 0.034	2.798GluTyr: 2.798 ± 0.063	0.002GluXaa: 0.002 ± 0.002
Phe	2.961PheAla: 2.961 ± 0.064	0.797PheCys: 0.797 ± 0.032	2.324PheAsp: 2.324 ± 0.055	2.398PheGlu: 2.398 ± 0.056	1.908PhePhe: 1.908 ± 0.058	3.301PheGly: 3.301 ± 0.086	0.864PheHis: 0.864 ± 0.032	2.454PheIle: 2.454 ± 0.061	1.885PheLys: 1.885 ± 0.046	4.37PheLeu: 4.37 ± 0.085	1.28PheMet: 1.28 ± 0.044	1.314PheAsn: 1.314 ± 0.045	1.58PhePro: 1.58 ± 0.047	1.338PheGln: 1.338 ± 0.038	1.878PheArg: 1.878 ± 0.044	3.012PheSer: 3.012 ± 0.064	2.312PheThr: 2.312 ± 0.057	2.71PheVal: 2.71 ± 0.061	0.404PheTrp: 0.404 ± 0.023	1.751PheTyr: 1.751 ± 0.045	0.001PheXaa: 0.001 ± 0.001
Gly	6.277GlyAla: 6.277 ± 0.102	1.458GlyCys: 1.458 ± 0.054	4.054GlyAsp: 4.054 ± 0.074	5.404GlyGlu: 5.404 ± 0.088	3.429GlyPhe: 3.429 ± 0.064	6.236GlyGly: 6.236 ± 0.117	1.497GlyHis: 1.497 ± 0.047	6.241GlyIle: 6.241 ± 0.107	5.548GlyLys: 5.548 ± 0.091	7.058GlyLeu: 7.058 ± 0.112	2.752GlyMet: 2.752 ± 0.068	3.027GlyAsn: 3.027 ± 0.077	2.049GlyPro: 2.049 ± 0.055	2.499GlyGln: 2.499 ± 0.065	4.385GlyArg: 4.385 ± 0.095	4.87GlySer: 4.87 ± 0.095	4.906GlyThr: 4.906 ± 0.084	5.339GlyVal: 5.339 ± 0.095	0.93GlyTrp: 0.93 ± 0.042	3.227GlyTyr: 3.227 ± 0.06	0.012GlyXaa: 0.012 ± 0.004
His	1.148HisAla: 1.148 ± 0.04	0.289HisCys: 0.289 ± 0.022	0.898HisAsp: 0.898 ± 0.035	1.13HisGlu: 1.13 ± 0.039	0.749HisPhe: 0.749 ± 0.03	1.577HisGly: 1.577 ± 0.045	0.422HisHis: 0.422 ± 0.029	1.197HisIle: 1.197 ± 0.041	0.863HisLys: 0.863 ± 0.032	1.516HisLeu: 1.516 ± 0.05	0.662HisMet: 0.662 ± 0.032	0.61HisAsn: 0.61 ± 0.029	0.974HisPro: 0.974 ± 0.039	0.487HisGln: 0.487 ± 0.024	0.897HisArg: 0.897 ± 0.035	0.905HisSer: 0.905 ± 0.036	0.951HisThr: 0.951 ± 0.035	1.309HisVal: 1.309 ± 0.04	0.173HisTrp: 0.173 ± 0.013	0.647HisTyr: 0.647 ± 0.025	0.0HisXaa: 0.0 ± 0.0
Ile	5.031IleAla: 5.031 ± 0.102	1.218IleCys: 1.218 ± 0.041	3.299IleAsp: 3.299 ± 0.064	3.532IleGlu: 3.532 ± 0.088	2.602IlePhe: 2.602 ± 0.07	4.808IleGly: 4.808 ± 0.088	1.316IleHis: 1.316 ± 0.044	3.772IleIle: 3.772 ± 0.073	2.916IleLys: 2.916 ± 0.063	6.821IleLeu: 6.821 ± 0.111	1.747IleMet: 1.747 ± 0.043	2.221IleAsn: 2.221 ± 0.056	3.338IlePro: 3.338 ± 0.072	2.3IleGln: 2.3 ± 0.059	4.17IleArg: 4.17 ± 0.088	4.187IleSer: 4.187 ± 0.082	3.567IleThr: 3.567 ± 0.075	4.128IleVal: 4.128 ± 0.077	0.579IleTrp: 0.579 ± 0.026	2.177IleTyr: 2.177 ± 0.062	0.0IleXaa: 0.0 ± 0.0
Lys	4.959LysAla: 4.959 ± 0.087	0.534LysCys: 0.534 ± 0.027	3.284LysAsp: 3.284 ± 0.07	6.126LysGlu: 6.126 ± 0.109	1.61LysPhe: 1.61 ± 0.047	3.943LysGly: 3.943 ± 0.077	0.8LysHis: 0.8 ± 0.03	3.665LysIle: 3.665 ± 0.073	5.322LysLys: 5.322 ± 0.085	4.555LysLeu: 4.555 ± 0.08	1.868LysMet: 1.868 ± 0.048	2.904LysAsn: 2.904 ± 0.063	1.956LysPro: 1.956 ± 0.056	1.806LysGln: 1.806 ± 0.056	3.128LysArg: 3.128 ± 0.064	2.526LysSer: 2.526 ± 0.056	3.466LysThr: 3.466 ± 0.065	3.357LysVal: 3.357 ± 0.065	0.502LysTrp: 0.502 ± 0.025	2.139LysTyr: 2.139 ± 0.05	0.0LysXaa: 0.0 ± 0.0
Leu	7.989LeuAla: 7.989 ± 0.106	1.497LeuCys: 1.497 ± 0.045	5.235LeuAsp: 5.235 ± 0.089	7.069LeuGlu: 7.069 ± 0.11	4.0LeuPhe: 4.0 ± 0.081	6.986LeuGly: 6.986 ± 0.109	1.547LeuHis: 1.547 ± 0.048	4.966LeuIle: 4.966 ± 0.092	6.108LeuLys: 6.108 ± 0.09	8.92LeuLeu: 8.92 ± 0.149	2.885LeuMet: 2.885 ± 0.058	3.26LeuAsn: 3.26 ± 0.065	3.926LeuPro: 3.926 ± 0.069	2.271LeuGln: 2.271 ± 0.059	4.136LeuArg: 4.136 ± 0.078	6.448LeuSer: 6.448 ± 0.101	4.985LeuThr: 4.985 ± 0.082	5.958LeuVal: 5.958 ± 0.106	0.869LeuTrp: 0.869 ± 0.034	3.338LeuTyr: 3.338 ± 0.065	0.001LeuXaa: 0.001 ± 0.001
Met	3.243MetAla: 3.243 ± 0.068	0.342MetCys: 0.342 ± 0.023	2.26MetAsp: 2.26 ± 0.053	3.269MetGlu: 3.269 ± 0.071	1.054MetPhe: 1.054 ± 0.039	2.63MetGly: 2.63 ± 0.067	0.422MetHis: 0.422 ± 0.022	2.062MetIle: 2.062 ± 0.05	2.425MetLys: 2.425 ± 0.054	2.414MetLeu: 2.414 ± 0.061	0.986MetMet: 0.986 ± 0.037	1.411MetAsn: 1.411 ± 0.042	1.134MetPro: 1.134 ± 0.034	0.842MetGln: 0.842 ± 0.033	1.384MetArg: 1.384 ± 0.046	1.574MetSer: 1.574 ± 0.044	2.014MetThr: 2.014 ± 0.047	2.288MetVal: 2.288 ± 0.058	0.229MetTrp: 0.229 ± 0.016	0.853MetTyr: 0.853 ± 0.031	0.0MetXaa: 0.0 ± 0.0
Asn	2.85AsnAla: 2.85 ± 0.06	0.535AsnCys: 0.535 ± 0.028	1.688AsnAsp: 1.688 ± 0.047	2.196AsnGlu: 2.196 ± 0.055	1.282AsnPhe: 1.282 ± 0.041	3.612AsnGly: 3.612 ± 0.08	0.661AsnHis: 0.661 ± 0.029	2.418AsnIle: 2.418 ± 0.061	1.716AsnLys: 1.716 ± 0.049	3.173AsnLeu: 3.173 ± 0.071	1.23AsnMet: 1.23 ± 0.04	1.22AsnAsn: 1.22 ± 0.047	1.826AsnPro: 1.826 ± 0.05	1.171AsnGln: 1.171 ± 0.041	1.893AsnArg: 1.893 ± 0.051	1.925AsnSer: 1.925 ± 0.053	1.849AsnThr: 1.849 ± 0.05	2.562AsnVal: 2.562 ± 0.069	0.319AsnTrp: 0.319 ± 0.021	1.326AsnTyr: 1.326 ± 0.041	0.0AsnXaa: 0.0 ± 0.0
Pro	3.364ProAla: 3.364 ± 0.069	0.494ProCys: 0.494 ± 0.027	2.419ProAsp: 2.419 ± 0.053	4.311ProGlu: 4.311 ± 0.072	1.602ProPhe: 1.602 ± 0.048	3.584ProGly: 3.584 ± 0.084	0.652ProHis: 0.652 ± 0.031	1.911ProIle: 1.911 ± 0.058	1.524ProLys: 1.524 ± 0.042	3.074ProLeu: 3.074 ± 0.062	1.155ProMet: 1.155 ± 0.039	0.994ProAsn: 0.994 ± 0.043	1.006ProPro: 1.006 ± 0.043	1.094ProGln: 1.094 ± 0.037	1.53ProArg: 1.53 ± 0.045	2.207ProSer: 2.207 ± 0.058	1.423ProThr: 1.423 ± 0.041	3.757ProVal: 3.757 ± 0.069	0.436ProTrp: 0.436 ± 0.024	1.51ProTyr: 1.51 ± 0.051	0.004ProXaa: 0.004 ± 0.002
Gln	2.691GlnAla: 2.691 ± 0.064	0.409GlnCys: 0.409 ± 0.026	1.49GlnAsp: 1.49 ± 0.048	2.413GlnGlu: 2.413 ± 0.053	1.131GlnPhe: 1.131 ± 0.039	2.059GlnGly: 2.059 ± 0.055	0.394GlnHis: 0.394 ± 0.02	2.214GlnIle: 2.214 ± 0.062	2.175GlnLys: 2.175 ± 0.052	2.381GlnLeu: 2.381 ± 0.046	1.156GlnMet: 1.156 ± 0.038	1.216GlnAsn: 1.216 ± 0.044	0.885GlnPro: 0.885 ± 0.033	0.772GlnGln: 0.772 ± 0.034	1.321GlnArg: 1.321 ± 0.042	1.452GlnSer: 1.452 ± 0.045	1.583GlnThr: 1.583 ± 0.049	2.317GlnVal: 2.317 ± 0.058	0.391GlnTrp: 0.391 ± 0.021	1.336GlnTyr: 1.336 ± 0.045	0.0GlnXaa: 0.0 ± 0.0
Arg	3.657ArgAla: 3.657 ± 0.072	0.679ArgCys: 0.679 ± 0.033	2.781ArgAsp: 2.781 ± 0.072	4.835ArgGlu: 4.835 ± 0.102	2.24ArgPhe: 2.24 ± 0.059	3.135ArgGly: 3.135 ± 0.06	0.832ArgHis: 0.832 ± 0.037	3.961ArgIle: 3.961 ± 0.071	3.407ArgLys: 3.407 ± 0.069	5.099ArgLeu: 5.099 ± 0.097	1.844ArgMet: 1.844 ± 0.043	1.93ArgAsn: 1.93 ± 0.047	1.918ArgPro: 1.918 ± 0.059	1.848ArgGln: 1.848 ± 0.049	3.145ArgArg: 3.145 ± 0.077	2.729ArgSer: 2.729 ± 0.06	2.695ArgThr: 2.695 ± 0.057	3.152ArgVal: 3.152 ± 0.062	0.431ArgTrp: 0.431 ± 0.025	2.094ArgTyr: 2.094 ± 0.053	0.006ArgXaa: 0.006 ± 0.003
Ser	4.529SerAla: 4.529 ± 0.084	0.866SerCys: 0.866 ± 0.033	2.842SerAsp: 2.842 ± 0.065	3.389SerGlu: 3.389 ± 0.069	2.653SerPhe: 2.653 ± 0.06	5.71SerGly: 5.71 ± 0.086	1.048SerHis: 1.048 ± 0.04	3.617SerIle: 3.617 ± 0.077	2.13SerLys: 2.13 ± 0.051	5.561SerLeu: 5.561 ± 0.087	2.086SerMet: 2.086 ± 0.049	1.659SerAsn: 1.659 ± 0.056	2.014SerPro: 2.014 ± 0.046	1.978SerGln: 1.978 ± 0.056	3.654SerArg: 3.654 ± 0.072	3.588SerSer: 3.588 ± 0.086	2.563SerThr: 2.563 ± 0.055	4.323SerVal: 4.323 ± 0.085	0.652SerTrp: 0.652 ± 0.03	2.074SerTyr: 2.074 ± 0.049	0.005SerXaa: 0.005 ± 0.002
Thr	5.173ThrAla: 5.173 ± 0.086	0.622ThrCys: 0.622 ± 0.026	3.045ThrAsp: 3.045 ± 0.056	3.925ThrGlu: 3.925 ± 0.08	2.089ThrPhe: 2.089 ± 0.055	5.345ThrGly: 5.345 ± 0.087	0.812ThrHis: 0.812 ± 0.035	3.327ThrIle: 3.327 ± 0.069	2.398ThrLys: 2.398 ± 0.055	4.488ThrLeu: 4.488 ± 0.072	1.526ThrMet: 1.526 ± 0.04	1.488ThrAsn: 1.488 ± 0.038	2.31ThrPro: 2.31 ± 0.058	1.189ThrGln: 1.189 ± 0.041	2.259ThrArg: 2.259 ± 0.057	2.69ThrSer: 2.69 ± 0.061	2.411ThrThr: 2.411 ± 0.048	4.85ThrVal: 4.85 ± 0.097	0.545ThrTrp: 0.545 ± 0.029	1.822ThrTyr: 1.822 ± 0.054	0.002ThrXaa: 0.002 ± 0.001
Val	4.795ValAla: 4.795 ± 0.099	1.243ValCys: 1.243 ± 0.04	3.828ValAsp: 3.828 ± 0.066	4.605ValGlu: 4.605 ± 0.087	3.284ValPhe: 3.284 ± 0.066	4.549ValGly: 4.549 ± 0.083	1.154ValHis: 1.154 ± 0.034	4.927ValIle: 4.927 ± 0.087	4.106ValLys: 4.106 ± 0.078	7.368ValLeu: 7.368 ± 0.111	2.286ValMet: 2.286 ± 0.056	2.588ValAsn: 2.588 ± 0.051	3.028ValPro: 3.028 ± 0.061	1.942ValGln: 1.942 ± 0.048	3.587ValArg: 3.587 ± 0.06	4.703ValSer: 4.703 ± 0.078	4.35ValThr: 4.35 ± 0.083	4.984ValVal: 4.984 ± 0.085	0.695ValTrp: 0.695 ± 0.027	2.683ValTyr: 2.683 ± 0.067	0.004ValXaa: 0.004 ± 0.002
Trp	0.641TrpAla: 0.641 ± 0.031	0.145TrpCys: 0.145 ± 0.014	0.559TrpAsp: 0.559 ± 0.026	0.76TrpGlu: 0.76 ± 0.027	0.427TrpPhe: 0.427 ± 0.02	0.708TrpGly: 0.708 ± 0.03	0.154TrpHis: 0.154 ± 0.013	0.72TrpIle: 0.72 ± 0.027	0.745TrpLys: 0.745 ± 0.031	0.902TrpLeu: 0.902 ± 0.038	0.355TrpMet: 0.355 ± 0.022	0.448TrpAsn: 0.448 ± 0.022	0.289TrpPro: 0.289 ± 0.021	0.327TrpGln: 0.327 ± 0.023	0.477TrpArg: 0.477 ± 0.025	0.477TrpSer: 0.477 ± 0.028	0.468TrpThr: 0.468 ± 0.023	0.544TrpVal: 0.544 ± 0.025	0.115TrpTrp: 0.115 ± 0.012	0.433TrpTyr: 0.433 ± 0.028	0.001TrpXaa: 0.001 ± 0.001
Tyr	2.575TyrAla: 2.575 ± 0.057	0.515TyrCys: 0.515 ± 0.025	2.293TyrAsp: 2.293 ± 0.049	2.758TyrGlu: 2.758 ± 0.061	1.72TyrPhe: 1.72 ± 0.052	3.244TyrGly: 3.244 ± 0.074	0.755TyrHis: 0.755 ± 0.029	2.16TyrIle: 2.16 ± 0.051	1.862TyrLys: 1.862 ± 0.05	3.475TyrLeu: 3.475 ± 0.072	1.177TyrMet: 1.177 ± 0.039	1.406TyrAsn: 1.406 ± 0.04	1.474TyrPro: 1.474 ± 0.04	1.195TyrGln: 1.195 ± 0.04	2.074TyrArg: 2.074 ± 0.05	2.083TyrSer: 2.083 ± 0.052	2.014TyrThr: 2.014 ± 0.058	2.444TyrVal: 2.444 ± 0.056	0.355TyrTrp: 0.355 ± 0.024	1.69TyrTyr: 1.69 ± 0.067	0.001TyrXaa: 0.001 ± 0.001
Xaa	0.004XaaAla: 0.004 ± 0.002	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.001XaaGlu: 0.001 ± 0.001	0.001XaaPhe: 0.001 ± 0.001	0.01XaaGly: 0.01 ± 0.003	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.001XaaLys: 0.001 ± 0.001	0.002XaaLeu: 0.002 ± 0.002	0.002XaaMet: 0.002 ± 0.002	0.0XaaAsn: 0.0 ± 0.0	0.007XaaPro: 0.007 ± 0.003	0.002XaaGln: 0.002 ± 0.002	0.011XaaArg: 0.011 ± 0.003	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.005XaaVal: 0.005 ± 0.003	0.001XaaTrp: 0.001 ± 0.001	0.0XaaTyr: 0.0 ± 0.0	0.065XaaXaa: 0.065 ± 0.012

Statistics based on 2660 proteins (805197 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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