Plicaturopsis crispa FD-325 SS-3
Average proteome isoelectric point is 6.97
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2710 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0C9SRR0|A0A0C9SRR0_PLICR Unplaced genomic scaffold PLICRscaffold_16 whole genome shotgun sequence OS=Plicaturopsis crispa FD-325 SS-3 OX=944288 GN=PLICRDRAFT_348688 PE=4 SV=1
MM1 pKa = 8.14 DD2 pKa = 4.87 EE3 pKa = 6.4 DD4 pKa = 4.25 EE5 pKa = 6.18 DD6 pKa = 4.6 EE7 pKa = 6.13 DD8 pKa = 4.87 NIPDD12 pKa = 3.81 NYY14 pKa = 10.49 EE15 pKa = 4.12 DD16 pKa = 5.06 NIPDD20 pKa = 3.65 NYY22 pKa = 10.25 EE23 pKa = 3.52 DD24 pKa = 5.42 HH25 pKa = 7.23 IPDD28 pKa = 3.3 NYY30 pKa = 10.72 EE31 pKa = 3.59 FYY33 pKa = 10.79 LANDD37 pKa = 3.35 EE38 pKa = 4.95 HH39 pKa = 7.51 DD40 pKa = 4.11 IPDD43 pKa = 4.2 LLRR46 pKa = 11.84 FSRR49 pKa = 11.84 PRR51 pKa = 11.84 EE52 pKa = 3.7 RR53 pKa = 11.84 LPSIFGDD60 pKa = 3.65 VCFTPLPSTLDD71 pKa = 3.58 LQPQPSHH78 pKa = 6.26 PHH80 pKa = 6.12 PPGCEE85 pKa = 3.61 WGRR88 pKa = 11.84 GDD90 pKa = 4.36
Molecular weight: 10.51 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.739
IPC2_protein 3.846
IPC_protein 3.834
Toseland 3.617
ProMoST 3.948
Dawson 3.834
Bjellqvist 4.024
Wikipedia 3.77
Rodwell 3.668
Grimsley 3.528
Solomon 3.821
Lehninger 3.77
Nozaki 3.948
DTASelect 4.177
Thurlkill 3.681
EMBOSS 3.783
Sillero 3.961
Patrickios 1.901
IPC_peptide 3.808
IPC2_peptide 3.935
IPC2.peptide.svr19 3.869
Protein with the highest isoelectric point:
>tr|A0A0C9SVY9|A0A0C9SVY9_PLICR Unplaced genomic scaffold PLICRscaffold_25 whole genome shotgun sequence OS=Plicaturopsis crispa FD-325 SS-3 OX=944288 GN=PLICRDRAFT_47231 PE=3 SV=1
MM1 pKa = 7.49 FFFLFQPPGARR12 pKa = 11.84 RR13 pKa = 11.84 RR14 pKa = 11.84 SRR16 pKa = 11.84 PRR18 pKa = 11.84 AQSRR22 pKa = 11.84 AAPPRR27 pKa = 11.84 TPPPPRR33 pKa = 11.84 YY34 pKa = 8.71 PPQSPPPPPSLSPVPASATSRR55 pKa = 11.84 RR56 pKa = 11.84 IATTTPSALPTRR68 pKa = 11.84 SPPRR72 pKa = 11.84 TPPSSPTRR80 pKa = 11.84 PSSSSPPTPPP90 pKa = 3.68
Molecular weight: 9.62 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.451
IPC2_protein 11.169
IPC_protein 12.588
Toseland 12.735
ProMoST 13.247
Dawson 12.735
Bjellqvist 12.735
Wikipedia 13.217
Rodwell 12.237
Grimsley 12.778
Solomon 13.247
Lehninger 13.144
Nozaki 12.735
DTASelect 12.735
Thurlkill 12.735
EMBOSS 13.247
Sillero 12.735
Patrickios 11.974
IPC_peptide 13.247
IPC2_peptide 12.237
IPC2.peptide.svr19 9.151
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2709
1
2710
993639
49
3923
366.7
40.44
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.758 ± 0.052
1.437 ± 0.026
5.701 ± 0.043
5.541 ± 0.05
3.496 ± 0.036
6.48 ± 0.048
2.842 ± 0.027
4.391 ± 0.037
4.043 ± 0.044
8.85 ± 0.062
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.019 ± 0.017
3.059 ± 0.027
7.038 ± 0.071
3.425 ± 0.028
7.204 ± 0.055
8.27 ± 0.068
6.038 ± 0.035
6.311 ± 0.038
1.522 ± 0.019
2.573 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here