candidate division MSBL1 archaeon SCGC-AAA382A20

Taxonomy: cellular organisms; Archaea; Euryarchaeota; Euryarchaeota incertae sedis; candidate division MSBL1

Average proteome isoelectric point is 6.26

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 947 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A133VLC1|A0A133VLC1_9EURY Uncharacterized protein OS=candidate division MSBL1 archaeon SCGC-AAA382A20 OX=1698280 GN=AKJ51_01725 PE=4 SV=1
MM1 pKa = 7.44KK2 pKa = 10.34KK3 pKa = 10.3KK4 pKa = 10.33IGIFAIAAVVIGALVLPGSPVAFASGWDD32 pKa = 3.47DD33 pKa = 4.06CPFGRR38 pKa = 11.84TNDD41 pKa = 4.3PYY43 pKa = 10.62PGHH46 pKa = 7.05CSRR49 pKa = 11.84YY50 pKa = 9.37IDD52 pKa = 3.67TDD54 pKa = 3.07NDD56 pKa = 4.96GICDD60 pKa = 3.83HH61 pKa = 6.46SQPAPEE67 pKa = 5.07DD68 pKa = 3.99RR69 pKa = 11.84NDD71 pKa = 3.97APTADD76 pKa = 3.64ADD78 pKa = 3.89GNYY81 pKa = 10.33SVTEE85 pKa = 4.17GGSISLDD92 pKa = 3.2GSGSSDD98 pKa = 3.1SDD100 pKa = 3.76GSISSYY106 pKa = 10.23FWTITLGGTYY116 pKa = 10.46GSLDD120 pKa = 3.7STSGSSPGFSATSEE134 pKa = 4.23TGDD137 pKa = 3.6HH138 pKa = 6.69SSHH141 pKa = 6.97DD142 pKa = 3.72VTVEE146 pKa = 3.78LTVTDD151 pKa = 4.64DD152 pKa = 5.8DD153 pKa = 5.0GATDD157 pKa = 3.62TDD159 pKa = 4.11TATVTVNSQNDD170 pKa = 3.8PPTTSSDD177 pKa = 4.33SITTCEE183 pKa = 4.04NTSGSSDD190 pKa = 3.49VLFNDD195 pKa = 4.15SDD197 pKa = 4.38PEE199 pKa = 5.08GDD201 pKa = 3.56SLVYY205 pKa = 10.91SNVSISTSPSHH216 pKa = 6.57GSASVDD222 pKa = 3.33EE223 pKa = 4.47TAGEE227 pKa = 4.03IDD229 pKa = 4.0YY230 pKa = 11.35SPNPNYY236 pKa = 10.54NGSDD240 pKa = 3.09SFEE243 pKa = 4.07YY244 pKa = 10.46QISDD248 pKa = 3.56GNGGISTEE256 pKa = 4.41TVSVTVNNVSDD267 pKa = 3.86SPTASFTHH275 pKa = 6.09SSSDD279 pKa = 3.18KK280 pKa = 11.07VLDD283 pKa = 3.53VDD285 pKa = 4.94ASNSSDD291 pKa = 2.94IDD293 pKa = 3.81GSISSYY299 pKa = 6.88EE300 pKa = 4.21WKK302 pKa = 9.82WSSGDD307 pKa = 3.62SFSSGTEE314 pKa = 4.13TDD316 pKa = 2.85SHH318 pKa = 6.55TYY320 pKa = 10.35SSGGEE325 pKa = 3.92YY326 pKa = 9.73TVEE329 pKa = 4.7LRR331 pKa = 11.84VTDD334 pKa = 4.67DD335 pKa = 5.01DD336 pKa = 4.81GATDD340 pKa = 4.1TYY342 pKa = 10.98SQTVTINKK350 pKa = 10.13DD351 pKa = 2.68SDD353 pKa = 3.95APSSLSPSDD362 pKa = 3.6GVKK365 pKa = 10.53LSEE368 pKa = 4.25TSSVTLEE375 pKa = 4.22VTVTDD380 pKa = 4.27PDD382 pKa = 4.27GDD384 pKa = 4.17SMDD387 pKa = 3.29VAFYY391 pKa = 10.98DD392 pKa = 4.51GGGTQIGTTQTGVSDD407 pKa = 3.95GGTTSVSYY415 pKa = 10.65SVSAGNSYY423 pKa = 11.1DD424 pKa = 3.2WYY426 pKa = 11.07AVATDD431 pKa = 4.66DD432 pKa = 5.13EE433 pKa = 5.02GASTQSSTWSFTVKK447 pKa = 10.49PPVDD451 pKa = 3.4EE452 pKa = 4.68SKK454 pKa = 11.34EE455 pKa = 3.78EE456 pKa = 3.98DD457 pKa = 4.38EE458 pKa = 5.76IIQTYY463 pKa = 8.13TLLPILIVLSLSCSITLFLSKK484 pKa = 10.22RR485 pKa = 11.84KK486 pKa = 10.04SISTLTHH493 pKa = 4.93KK494 pKa = 10.69RR495 pKa = 11.84IWNVFLLITFLASGILGILLVIRR518 pKa = 11.84INFGFVIPLPFNVLFWHH535 pKa = 6.16VEE537 pKa = 3.36AGIAMFVICIFHH549 pKa = 6.57IIEE552 pKa = 4.34RR553 pKa = 11.84FRR555 pKa = 11.84ALLWFLL561 pKa = 4.05

Molecular weight: 59.2 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A133VJL8|A0A133VJL8_9EURY Uncharacterized protein OS=candidate division MSBL1 archaeon SCGC-AAA382A20 OX=1698280 GN=AKJ51_03325 PE=4 SV=1
MM1 pKa = 7.18TSAGFHH7 pKa = 6.8LFQMIPKK14 pKa = 10.02KK15 pKa = 10.69YY16 pKa = 9.16LNCRR20 pKa = 11.84NGPIPNIGPNRR31 pKa = 11.84QRR33 pKa = 11.84EE34 pKa = 4.38GFHH37 pKa = 5.89FHH39 pKa = 7.25LFTFADD45 pKa = 3.87PLFNPGSRR53 pKa = 11.84KK54 pKa = 9.06FVCSTLRR61 pKa = 11.84RR62 pKa = 11.84DD63 pKa = 3.3LRR65 pKa = 11.84EE66 pKa = 3.92YY67 pKa = 10.17IARR70 pKa = 11.84SSSQWLPRR78 pKa = 11.84QDD80 pKa = 3.49RR81 pKa = 11.84PRR83 pKa = 11.84EE84 pKa = 4.08GPEE87 pKa = 3.74GSSYY91 pKa = 11.06RR92 pKa = 11.84GFKK95 pKa = 10.72GSTFSSSSPLPQFNGGG111 pKa = 3.39

Molecular weight: 12.67 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

947

0

947

216303

38

1910

228.4

25.87

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.524 ± 0.074

1.166 ± 0.045

6.114 ± 0.07

10.1 ± 0.142

3.94 ± 0.063

7.175 ± 0.081

1.748 ± 0.036

6.937 ± 0.071

8.11 ± 0.125

8.726 ± 0.096

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.22 ± 0.039

4.343 ± 0.089

3.927 ± 0.053

2.488 ± 0.044

5.38 ± 0.085

6.458 ± 0.088

4.732 ± 0.081

6.567 ± 0.07

1.179 ± 0.04

3.166 ± 0.056
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski