Erythrobacter neustonensis

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Sphingomonadales; Erythrobacteraceae; Erythrobacter/Porphyrobacter group; Erythrobacter

Average proteome isoelectric point is 6.29

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2792 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A192D498|A0A192D498_9SPHN Flagellar biosynthetic protein FliQ OS=Erythrobacter neustonensis OX=1112 GN=A9D12_06120 PE=3 SV=1
MM1 pKa = 7.82FIRR4 pKa = 11.84FASAASAVALSACFAAPALAGEE26 pKa = 4.37ADD28 pKa = 3.59AAIVGPAAEE37 pKa = 4.26TEE39 pKa = 4.14IMISEE44 pKa = 4.61TAADD48 pKa = 3.95TAAGTPARR56 pKa = 11.84APINLDD62 pKa = 3.16EE63 pKa = 5.09ATGQDD68 pKa = 3.66DD69 pKa = 4.12AAPALTVSGSATLASDD85 pKa = 3.31YY86 pKa = 10.66RR87 pKa = 11.84FRR89 pKa = 11.84GVSQTDD95 pKa = 2.52EE96 pKa = 4.03GMAVQGGVTLSHH108 pKa = 6.67SSGFYY113 pKa = 9.84FGAWGSNLAGWGTFGGANMEE133 pKa = 4.4LDD135 pKa = 3.99LFAGFKK141 pKa = 10.62VPVGEE146 pKa = 4.34GTLDD150 pKa = 5.02LGGTWYY156 pKa = 9.0MYY158 pKa = 10.48PSGADD163 pKa = 3.06EE164 pKa = 4.11TDD166 pKa = 3.44FAEE169 pKa = 5.78LYY171 pKa = 10.73AKK173 pKa = 10.75LSGTAGPLGLTATVAYY189 pKa = 10.1APKK192 pKa = 10.39QEE194 pKa = 4.19ALGNAFPVGLPADD207 pKa = 4.6PGDD210 pKa = 4.59KK211 pKa = 10.46EE212 pKa = 4.68DD213 pKa = 3.98NLYY216 pKa = 10.87LAGDD220 pKa = 3.8AVYY223 pKa = 10.43AVGDD227 pKa = 3.98SPVSLKK233 pKa = 11.05AHH235 pKa = 6.75IGYY238 pKa = 9.98SDD240 pKa = 3.96GNPGLGPNGTSIAPTGTYY258 pKa = 10.19FDD260 pKa = 4.04WLVGIDD266 pKa = 4.56FVATPGLTFSAAYY279 pKa = 9.99VDD281 pKa = 3.94TDD283 pKa = 3.12ISEE286 pKa = 4.81AEE288 pKa = 4.13ANRR291 pKa = 11.84IRR293 pKa = 11.84PNFATLDD300 pKa = 3.76GNSISDD306 pKa = 3.53ATVVLSVTASFF317 pKa = 4.2

Molecular weight: 32.09 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A192D0T9|A0A192D0T9_9SPHN Uncharacterized protein OS=Erythrobacter neustonensis OX=1112 GN=A9D12_01055 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.61RR3 pKa = 11.84TFQPSNLVRR12 pKa = 11.84ARR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.89GFFARR21 pKa = 11.84KK22 pKa = 7.57ATPGGQKK29 pKa = 9.19VLRR32 pKa = 11.84ARR34 pKa = 11.84RR35 pKa = 11.84ARR37 pKa = 11.84GRR39 pKa = 11.84KK40 pKa = 8.81KK41 pKa = 10.46LCAA44 pKa = 3.96

Molecular weight: 5.08 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2792

0

2792

918342

29

1954

328.9

35.35

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

14.318 ± 0.081

0.791 ± 0.013

5.877 ± 0.038

5.564 ± 0.041

3.591 ± 0.033

9.019 ± 0.045

1.919 ± 0.025

4.969 ± 0.026

2.873 ± 0.034

9.9 ± 0.051

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.46 ± 0.022

2.488 ± 0.027

5.327 ± 0.031

3.097 ± 0.023

7.116 ± 0.044

4.867 ± 0.027

5.286 ± 0.035

7.064 ± 0.033

1.389 ± 0.02

2.085 ± 0.024
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski