Citrobacter phage Miller

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; Tevenvirinae; Pseudotevenvirus; Citrobacter virus Miller

Average proteome isoelectric point is 6.26

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 277 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A076YHV4|A0A076YHV4_9CAUD Head assembly protein OS=Citrobacter phage Miller OX=1527524 GN=CPTMiller_0037 PE=4 SV=1
MM1 pKa = 7.87SDD3 pKa = 3.85LLHH6 pKa = 7.39DD7 pKa = 4.41ILVAAYY13 pKa = 9.07PDD15 pKa = 3.8EE16 pKa = 4.48EE17 pKa = 4.89PEE19 pKa = 3.96FEE21 pKa = 4.17SPYY24 pKa = 8.54RR25 pKa = 11.84SEE27 pKa = 4.42WIVSVDD33 pKa = 3.28DD34 pKa = 4.06KK35 pKa = 11.53GYY37 pKa = 9.88VDD39 pKa = 3.87IMRR42 pKa = 11.84WPNIHH47 pKa = 6.96HH48 pKa = 7.13GYY50 pKa = 10.2LDD52 pKa = 3.45EE53 pKa = 5.48GINAEE58 pKa = 4.12MLGMPEE64 pKa = 4.11QVEE67 pKa = 4.57DD68 pKa = 3.58PAGVYY73 pKa = 9.7KK74 pKa = 9.65WICSPWEE81 pKa = 3.85TRR83 pKa = 11.84DD84 pKa = 3.21WEE86 pKa = 4.74SNIVDD91 pKa = 3.21DD92 pKa = 3.9WGFDD96 pKa = 3.74VVEE99 pKa = 4.3STPLFLLPEE108 pKa = 4.3KK109 pKa = 11.25VNGEE113 pKa = 4.19VNEE116 pKa = 4.68SGNQTTQQDD125 pKa = 3.74NGSGGVV131 pKa = 3.21

Molecular weight: 14.89 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A076YMJ3|A0A076YMJ3_9CAUD J domain-containing protein OS=Citrobacter phage Miller OX=1527524 GN=CPTMiller_0058 PE=4 SV=1
MM1 pKa = 7.93RR2 pKa = 11.84IAVLGGTMISRR13 pKa = 11.84TLVSCLIDD21 pKa = 3.53SIRR24 pKa = 11.84WVNTPCVDD32 pKa = 3.44LCSRR36 pKa = 11.84IPYY39 pKa = 9.52EE40 pKa = 4.03EE41 pKa = 3.94PTPYY45 pKa = 9.97KK46 pKa = 10.14VKK48 pKa = 10.04PYY50 pKa = 9.6KK51 pKa = 10.42APRR54 pKa = 11.84INRR57 pKa = 11.84ALHH60 pKa = 5.1RR61 pKa = 11.84QSVNRR66 pKa = 11.84GKK68 pKa = 10.66

Molecular weight: 7.77 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

277

0

277

56527

30

1219

204.1

23.14

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.882 ± 0.17

1.162 ± 0.066

6.653 ± 0.108

6.906 ± 0.199

4.157 ± 0.096

6.259 ± 0.204

1.82 ± 0.079

6.558 ± 0.138

7.363 ± 0.2

7.701 ± 0.121

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.96 ± 0.097

5.449 ± 0.125

3.612 ± 0.087

3.232 ± 0.114

4.812 ± 0.113

5.758 ± 0.121

5.944 ± 0.225

7.097 ± 0.145

1.35 ± 0.056

4.324 ± 0.123
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski