Klebsiella phage 2b LV-2017

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.52

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 46 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2Z2FAA1|A0A2Z2FAA1_9CAUD Uncharacterized protein OS=Klebsiella phage 2b LV-2017 OX=1960656 PE=4 SV=1
MM1 pKa = 8.07ADD3 pKa = 4.79PITAADD9 pKa = 3.57VQAFLGEE16 pKa = 4.34LGYY19 pKa = 10.55SIPAALLDD27 pKa = 5.02PILCVVNKK35 pKa = 10.3IIPCLDD41 pKa = 2.89GAGYY45 pKa = 10.51DD46 pKa = 3.61DD47 pKa = 4.7CTAKK51 pKa = 10.83LILMYY56 pKa = 10.52AAALMATSSGARR68 pKa = 11.84RR69 pKa = 11.84IKK71 pKa = 10.75SQGAPSGASRR81 pKa = 11.84SFDD84 pKa = 3.58YY85 pKa = 11.32GDD87 pKa = 5.93DD88 pKa = 4.56GITWLRR94 pKa = 11.84DD95 pKa = 3.31SLAKK99 pKa = 10.37LDD101 pKa = 3.96TSGCTSEE108 pKa = 5.38LPISAGNSVGLFMVVGGCC126 pKa = 4.44

Molecular weight: 12.92 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2Z2FBY1|A0A2Z2FBY1_9CAUD Uncharacterized protein OS=Klebsiella phage 2b LV-2017 OX=1960656 PE=4 SV=1
MM1 pKa = 7.89RR2 pKa = 11.84KK3 pKa = 8.26PARR6 pKa = 11.84RR7 pKa = 11.84KK8 pKa = 9.32CAHH11 pKa = 5.64CRR13 pKa = 11.84EE14 pKa = 4.49WFHH17 pKa = 6.16PTRR20 pKa = 11.84EE21 pKa = 4.27GQVVCSFEE29 pKa = 4.09CASAIGKK36 pKa = 8.72KK37 pKa = 7.2QTAKK41 pKa = 10.56ARR43 pKa = 11.84EE44 pKa = 3.93AAKK47 pKa = 10.47ARR49 pKa = 11.84AVKK52 pKa = 10.09RR53 pKa = 11.84QRR55 pKa = 11.84EE56 pKa = 4.26SEE58 pKa = 4.0KK59 pKa = 10.31EE60 pKa = 3.66GRR62 pKa = 11.84QRR64 pKa = 11.84RR65 pKa = 11.84RR66 pKa = 11.84AKK68 pKa = 10.5RR69 pKa = 11.84EE70 pKa = 3.81SFKK73 pKa = 10.93TKK75 pKa = 10.43AQWDD79 pKa = 4.06KK80 pKa = 10.73EE81 pKa = 4.19AQSAFNRR88 pKa = 11.84YY89 pKa = 8.22IRR91 pKa = 11.84IRR93 pKa = 11.84DD94 pKa = 3.38EE95 pKa = 4.24GKK97 pKa = 9.03PCVSCGSPLIGKK109 pKa = 9.23SNYY112 pKa = 8.55LTGSAIDD119 pKa = 3.57ASHH122 pKa = 5.58YY123 pKa = 9.46RR124 pKa = 11.84SRR126 pKa = 11.84GAASHH131 pKa = 6.67LKK133 pKa = 9.12FNVFNVHH140 pKa = 6.17SACTRR145 pKa = 11.84CNRR148 pKa = 11.84QLSGNAVEE156 pKa = 4.08YY157 pKa = 10.25RR158 pKa = 11.84IRR160 pKa = 11.84LIEE163 pKa = 4.66RR164 pKa = 11.84IGLDD168 pKa = 2.75RR169 pKa = 11.84VEE171 pKa = 5.12RR172 pKa = 11.84LEE174 pKa = 4.4ADD176 pKa = 3.35NEE178 pKa = 3.87PRR180 pKa = 11.84RR181 pKa = 11.84FDD183 pKa = 3.05IPYY186 pKa = 8.71LQRR189 pKa = 11.84IKK191 pKa = 11.0SIFTRR196 pKa = 11.84RR197 pKa = 11.84ARR199 pKa = 11.84ALEE202 pKa = 3.86KK203 pKa = 10.4RR204 pKa = 11.84RR205 pKa = 11.84ARR207 pKa = 11.84HH208 pKa = 5.15QEE210 pKa = 3.52AAA212 pKa = 3.66

Molecular weight: 24.62 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

46

0

46

10595

56

1093

230.3

25.51

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.185 ± 0.818

1.265 ± 0.214

5.946 ± 0.338

6.437 ± 0.354

3.483 ± 0.248

7.126 ± 0.325

1.595 ± 0.241

5.267 ± 0.221

5.484 ± 0.346

7.532 ± 0.23

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.322 ± 0.173

4.266 ± 0.314

3.926 ± 0.311

4.587 ± 0.484

6.324 ± 0.393

6.305 ± 0.396

5.72 ± 0.403

6.437 ± 0.304

1.822 ± 0.22

2.973 ± 0.233
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski