Streptococcus satellite phage Javan591
Average proteome isoelectric point is 6.31
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 15 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4D5ZRM9|A0A4D5ZRM9_9VIRU Uncharacterized protein OS=Streptococcus satellite phage Javan591 OX=2558761 GN=JavanS591_0014 PE=4 SV=1
MM1 pKa = 6.48 TTLEE5 pKa = 3.79 EE6 pKa = 4.57 RR7 pKa = 11.84 YY8 pKa = 10.01 CIEE11 pKa = 3.34 RR12 pKa = 11.84 RR13 pKa = 11.84 AFLGQRR19 pKa = 11.84 ATRR22 pKa = 11.84 LEE24 pKa = 3.82 LLEE27 pKa = 4.82 RR28 pKa = 11.84 VICLEE33 pKa = 4.2 LMLMDD38 pKa = 4.55 MADD41 pKa = 3.52 STLDD45 pKa = 3.17 TDD47 pKa = 4.32 FPEE50 pKa = 4.44 MAVTTTLDD58 pKa = 3.62 HH59 pKa = 6.65 IADD62 pKa = 4.41 FGDD65 pKa = 3.32 EE66 pKa = 4.04 ALYY69 pKa = 10.92 LLEE72 pKa = 5.43 SLDD75 pKa = 3.97 HH76 pKa = 6.98 DD77 pKa = 4.84 GDD79 pKa = 4.35 YY80 pKa = 10.88 KK81 pKa = 11.34 ALWEE85 pKa = 4.2 HH86 pKa = 6.03 TKK88 pKa = 9.84 DD89 pKa = 3.16 TGADD93 pKa = 3.13 NGKK96 pKa = 9.96
Molecular weight: 11.02 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.332
IPC2_protein 4.266
IPC_protein 4.202
Toseland 4.012
ProMoST 4.342
Dawson 4.164
Bjellqvist 4.317
Wikipedia 4.075
Rodwell 4.037
Grimsley 3.923
Solomon 4.164
Lehninger 4.113
Nozaki 4.279
DTASelect 4.482
Thurlkill 4.05
EMBOSS 4.088
Sillero 4.317
Patrickios 3.643
IPC_peptide 4.164
IPC2_peptide 4.304
IPC2.peptide.svr19 4.235
Protein with the highest isoelectric point:
>tr|A0A4D5ZR67|A0A4D5ZR67_9VIRU HTH cro/C1-type domain-containing protein OS=Streptococcus satellite phage Javan591 OX=2558761 GN=JavanS591_0003 PE=4 SV=1
MM1 pKa = 7.19 NAVEE5 pKa = 5.08 PIRR8 pKa = 11.84 DD9 pKa = 3.55 KK10 pKa = 11.73 DD11 pKa = 4.28 DD12 pKa = 3.22 IQAMKK17 pKa = 10.58 DD18 pKa = 3.51 YY19 pKa = 10.63 LRR21 pKa = 11.84 SWNEE25 pKa = 3.0 RR26 pKa = 11.84 NYY28 pKa = 10.4 ILFLVGINTGFRR40 pKa = 11.84 IGDD43 pKa = 3.57 ILKK46 pKa = 10.51 LRR48 pKa = 11.84 VKK50 pKa = 10.27 DD51 pKa = 3.56 VQGWHH56 pKa = 5.22 IRR58 pKa = 11.84 VRR60 pKa = 11.84 EE61 pKa = 3.95 QKK63 pKa = 9.02 TGKK66 pKa = 9.53 QKK68 pKa = 10.45 NIKK71 pKa = 6.82 MTRR74 pKa = 11.84 TLKK77 pKa = 10.84 NEE79 pKa = 3.24 LRR81 pKa = 11.84 KK82 pKa = 9.86 YY83 pKa = 10.75 VEE85 pKa = 4.46 GKK87 pKa = 9.28 PLHH90 pKa = 6.77 HH91 pKa = 6.92 YY92 pKa = 10.12 LFQSRR97 pKa = 11.84 NGVNKK102 pKa = 9.67 PLDD105 pKa = 3.47 RR106 pKa = 11.84 RR107 pKa = 11.84 TVYY110 pKa = 10.13 WILKK114 pKa = 7.53 TAGNDD119 pKa = 3.58 LGIEE123 pKa = 4.31 NIGTHH128 pKa = 5.09 TMRR131 pKa = 11.84 KK132 pKa = 6.7 TFGYY136 pKa = 9.98 HH137 pKa = 5.21 YY138 pKa = 9.53 YY139 pKa = 10.76 KK140 pKa = 10.53 KK141 pKa = 10.06 YY142 pKa = 11.08 KK143 pKa = 10.39 NVADD147 pKa = 4.08 LMTIFNHH154 pKa = 6.01 SSPAITLIYY163 pKa = 10.12 IGVRR167 pKa = 11.84 QDD169 pKa = 4.72 DD170 pKa = 5.37 LDD172 pKa = 3.68 TKK174 pKa = 10.06 MANFDD179 pKa = 3.22 II180 pKa = 5.15
Molecular weight: 21.28 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.109
IPC2_protein 9.37
IPC_protein 9.355
Toseland 10.014
ProMoST 9.692
Dawson 10.218
Bjellqvist 9.867
Wikipedia 10.379
Rodwell 10.657
Grimsley 10.292
Solomon 10.233
Lehninger 10.204
Nozaki 9.97
DTASelect 9.867
Thurlkill 10.058
EMBOSS 10.409
Sillero 10.116
Patrickios 10.204
IPC_peptide 10.233
IPC2_peptide 8.361
IPC2.peptide.svr19 8.389
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
15
0
15
2318
42
435
154.5
18.04
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.997 ± 0.355
0.69 ± 0.204
6.212 ± 0.381
8.93 ± 1.188
4.443 ± 0.63
4.961 ± 0.37
2.243 ± 0.348
7.506 ± 0.704
9.362 ± 0.644
10.052 ± 0.713
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.2 ± 0.4
4.702 ± 0.465
2.761 ± 0.635
4.357 ± 0.348
5.22 ± 0.375
4.228 ± 0.386
5.522 ± 0.451
4.573 ± 0.271
1.337 ± 0.162
4.702 ± 0.27
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here