Achromobacter phage vB_AxyS_19-32_Axy06

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Steinhofvirus; unclassified Steinhofvirus

Average proteome isoelectric point is 6.58

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 55 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A514CTQ5|A0A514CTQ5_9CAUD Uncharacterized protein OS=Achromobacter phage vB_AxyS_19-32_Axy06 OX=2591049 GN=Axy06_047 PE=4 SV=1
MM1 pKa = 7.83DD2 pKa = 5.07RR3 pKa = 11.84ADD5 pKa = 4.31IEE7 pKa = 4.23DD8 pKa = 3.71LQRR11 pKa = 11.84VRR13 pKa = 11.84DD14 pKa = 3.94YY15 pKa = 11.16ICNEE19 pKa = 3.54LAMTDD24 pKa = 3.3EE25 pKa = 4.37MQRR28 pKa = 11.84LVSEE32 pKa = 4.11IEE34 pKa = 3.76LRR36 pKa = 11.84IATIEE41 pKa = 4.03VSIEE45 pKa = 3.78EE46 pKa = 4.21

Molecular weight: 5.46 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A514CTQ1|A0A514CTQ1_9CAUD Uncharacterized protein OS=Achromobacter phage vB_AxyS_19-32_Axy06 OX=2591049 GN=Axy06_048 PE=4 SV=1
MM1 pKa = 7.04TMSRR5 pKa = 11.84KK6 pKa = 9.66DD7 pKa = 3.49EE8 pKa = 4.26KK9 pKa = 10.77KK10 pKa = 10.5VRR12 pKa = 11.84EE13 pKa = 4.15LKK15 pKa = 10.85QMLEE19 pKa = 4.09YY20 pKa = 10.8ARR22 pKa = 11.84NEE24 pKa = 4.13LPNRR28 pKa = 11.84RR29 pKa = 11.84HH30 pKa = 5.37SMYY33 pKa = 9.63MHH35 pKa = 7.61TIRR38 pKa = 11.84MAVRR42 pKa = 11.84EE43 pKa = 4.34MIHH46 pKa = 5.49QRR48 pKa = 11.84IAARR52 pKa = 11.84SAEE55 pKa = 4.19LLAASAQRR63 pKa = 11.84AA64 pKa = 3.48

Molecular weight: 7.64 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

55

0

55

13835

46

1165

251.5

27.95

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.505 ± 0.33

0.846 ± 0.105

5.746 ± 0.266

6.599 ± 0.32

3.643 ± 0.217

7.756 ± 0.332

1.648 ± 0.172

5.045 ± 0.197

5.385 ± 0.426

8.204 ± 0.269

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.898 ± 0.149

4.113 ± 0.289

5.081 ± 0.259

4.236 ± 0.238

5.884 ± 0.212

5.674 ± 0.282

5.667 ± 0.346

7.04 ± 0.255

1.894 ± 0.202

3.13 ± 0.173
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski