Amino acid dipepetide frequency for Enterococcus phage phiFL3B

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
3.459AlaAla: 3.459 ± 1.053
0.329AlaCys: 0.329 ± 0.164
2.553AlaAsp: 2.553 ± 0.435
5.354AlaGlu: 5.354 ± 0.82
2.883AlaPhe: 2.883 ± 0.651
3.542AlaGly: 3.542 ± 0.511
0.659AlaHis: 0.659 ± 0.248
6.177AlaIle: 6.177 ± 0.633
5.766AlaLys: 5.766 ± 1.122
6.919AlaLeu: 6.919 ± 0.747
1.73AlaMet: 1.73 ± 0.317
3.624AlaAsn: 3.624 ± 0.556
1.235AlaPro: 1.235 ± 0.3
1.483AlaGln: 1.483 ± 0.367
2.224AlaArg: 2.224 ± 0.43
3.212AlaSer: 3.212 ± 0.824
4.612AlaThr: 4.612 ± 0.577
5.024AlaVal: 5.024 ± 0.664
1.483AlaTrp: 1.483 ± 0.619
2.883AlaTyr: 2.883 ± 0.569
0.0AlaXaa: 0.0 ± 0.0
Cys
0.247CysAla: 0.247 ± 0.175
0.0CysCys: 0.0 ± 0.0
0.165CysAsp: 0.165 ± 0.106
0.412CysGlu: 0.412 ± 0.155
0.329CysPhe: 0.329 ± 0.194
0.577CysGly: 0.577 ± 0.257
0.0CysHis: 0.0 ± 0.0
0.494CysIle: 0.494 ± 0.245
0.741CysLys: 0.741 ± 0.229
0.659CysLeu: 0.659 ± 0.217
0.0CysMet: 0.0 ± 0.0
0.247CysAsn: 0.247 ± 0.125
0.412CysPro: 0.412 ± 0.171
0.329CysGln: 0.329 ± 0.146
0.165CysArg: 0.165 ± 0.11
0.329CysSer: 0.329 ± 0.176
0.165CysThr: 0.165 ± 0.099
0.247CysVal: 0.247 ± 0.132
0.082CysTrp: 0.082 ± 0.086
0.247CysTyr: 0.247 ± 0.143
0.0CysXaa: 0.0 ± 0.0
Asp
4.201AspAla: 4.201 ± 0.828
0.247AspCys: 0.247 ± 0.135
4.118AspAsp: 4.118 ± 0.578
5.601AspGlu: 5.601 ± 0.714
2.471AspPhe: 2.471 ± 0.435
4.53AspGly: 4.53 ± 0.519
0.659AspHis: 0.659 ± 0.239
3.624AspIle: 3.624 ± 0.517
4.695AspLys: 4.695 ± 0.464
6.095AspLeu: 6.095 ± 0.85
1.4AspMet: 1.4 ± 0.359
2.8AspAsn: 2.8 ± 0.492
1.894AspPro: 1.894 ± 0.431
2.059AspGln: 2.059 ± 0.346
2.636AspArg: 2.636 ± 0.459
4.283AspSer: 4.283 ± 0.59
2.883AspThr: 2.883 ± 0.589
3.295AspVal: 3.295 ± 0.432
0.824AspTrp: 0.824 ± 0.249
2.389AspTyr: 2.389 ± 0.383
0.0AspXaa: 0.0 ± 0.0
Glu
4.695GluAla: 4.695 ± 0.858
0.741GluCys: 0.741 ± 0.269
3.212GluAsp: 3.212 ± 0.451
7.001GluGlu: 7.001 ± 1.236
3.13GluPhe: 3.13 ± 0.489
4.036GluGly: 4.036 ± 0.836
0.659GluHis: 0.659 ± 0.196
5.848GluIle: 5.848 ± 0.775
8.813GluLys: 8.813 ± 1.019
9.143GluLeu: 9.143 ± 1.413
2.553GluMet: 2.553 ± 0.396
4.201GluAsn: 4.201 ± 0.551
2.636GluPro: 2.636 ± 0.437
3.295GluGln: 3.295 ± 0.587
3.13GluArg: 3.13 ± 0.495
3.377GluSer: 3.377 ± 0.441
4.201GluThr: 4.201 ± 0.621
6.013GluVal: 6.013 ± 0.622
0.988GluTrp: 0.988 ± 0.229
2.8GluTyr: 2.8 ± 0.445
0.0GluXaa: 0.0 ± 0.0
Phe
2.8PheAla: 2.8 ± 0.366
0.329PheCys: 0.329 ± 0.144
3.295PheAsp: 3.295 ± 0.621
2.965PheGlu: 2.965 ± 0.566
1.483PhePhe: 1.483 ± 0.317
2.883PheGly: 2.883 ± 0.428
0.412PheHis: 0.412 ± 0.171
2.636PheIle: 2.636 ± 0.488
2.8PheLys: 2.8 ± 0.544
2.8PheLeu: 2.8 ± 0.417
1.153PheMet: 1.153 ± 0.254
2.471PheAsn: 2.471 ± 0.421
0.988PhePro: 0.988 ± 0.25
1.153PheGln: 1.153 ± 0.278
1.153PheArg: 1.153 ± 0.27
2.553PheSer: 2.553 ± 0.465
2.224PheThr: 2.224 ± 0.389
1.894PheVal: 1.894 ± 0.303
0.494PheTrp: 0.494 ± 0.185
1.565PheTyr: 1.565 ± 0.368
0.0PheXaa: 0.0 ± 0.0
Gly
4.777GlyAla: 4.777 ± 0.896
0.247GlyCys: 0.247 ± 0.119
3.624GlyAsp: 3.624 ± 0.599
4.365GlyGlu: 4.365 ± 0.602
2.718GlyPhe: 2.718 ± 0.455
3.377GlyGly: 3.377 ± 0.602
0.906GlyHis: 0.906 ± 0.301
6.177GlyIle: 6.177 ± 0.578
4.201GlyLys: 4.201 ± 0.576
5.107GlyLeu: 5.107 ± 0.769
1.318GlyMet: 1.318 ± 0.313
4.283GlyAsn: 4.283 ± 0.485
0.659GlyPro: 0.659 ± 0.185
2.718GlyGln: 2.718 ± 0.491
1.894GlyArg: 1.894 ± 0.377
4.695GlySer: 4.695 ± 0.895
3.459GlyThr: 3.459 ± 0.997
4.448GlyVal: 4.448 ± 0.67
0.494GlyTrp: 0.494 ± 0.172
3.13GlyTyr: 3.13 ± 0.529
0.0GlyXaa: 0.0 ± 0.0
His
0.659HisAla: 0.659 ± 0.288
0.247HisCys: 0.247 ± 0.192
0.659HisAsp: 0.659 ± 0.21
0.824HisGlu: 0.824 ± 0.276
0.494HisPhe: 0.494 ± 0.175
1.071HisGly: 1.071 ± 0.272
0.165HisHis: 0.165 ± 0.132
0.494HisIle: 0.494 ± 0.163
0.824HisLys: 0.824 ± 0.225
0.988HisLeu: 0.988 ± 0.407
0.165HisMet: 0.165 ± 0.096
0.412HisAsn: 0.412 ± 0.192
0.412HisPro: 0.412 ± 0.159
0.577HisGln: 0.577 ± 0.27
0.329HisArg: 0.329 ± 0.161
0.824HisSer: 0.824 ± 0.257
0.824HisThr: 0.824 ± 0.261
1.071HisVal: 1.071 ± 0.326
0.329HisTrp: 0.329 ± 0.159
1.071HisTyr: 1.071 ± 0.408
0.0HisXaa: 0.0 ± 0.0
Ile
4.695IleAla: 4.695 ± 0.757
0.659IleCys: 0.659 ± 0.195
5.189IleAsp: 5.189 ± 0.734
6.589IleGlu: 6.589 ± 0.789
2.883IlePhe: 2.883 ± 0.447
4.942IleGly: 4.942 ± 0.96
0.906IleHis: 0.906 ± 0.245
2.636IleIle: 2.636 ± 0.466
8.072IleLys: 8.072 ± 0.663
6.342IleLeu: 6.342 ± 0.736
1.153IleMet: 1.153 ± 0.362
3.789IleAsn: 3.789 ± 0.66
2.471IlePro: 2.471 ± 0.511
2.224IleGln: 2.224 ± 0.381
2.718IleArg: 2.718 ± 0.503
4.365IleSer: 4.365 ± 0.503
4.283IleThr: 4.283 ± 0.7
3.871IleVal: 3.871 ± 0.46
0.824IleTrp: 0.824 ± 0.275
2.224IleTyr: 2.224 ± 0.482
0.0IleXaa: 0.0 ± 0.0
Lys
6.589LysAla: 6.589 ± 0.702
0.329LysCys: 0.329 ± 0.15
4.612LysAsp: 4.612 ± 0.609
9.225LysGlu: 9.225 ± 1.074
2.636LysPhe: 2.636 ± 0.412
5.436LysGly: 5.436 ± 0.707
1.483LysHis: 1.483 ± 0.369
6.425LysIle: 6.425 ± 0.727
10.378LysLys: 10.378 ± 1.313
6.425LysLeu: 6.425 ± 0.733
2.718LysMet: 2.718 ± 0.514
6.013LysAsn: 6.013 ± 0.651
3.295LysPro: 3.295 ± 0.44
4.201LysGln: 4.201 ± 0.572
3.954LysArg: 3.954 ± 0.597
5.518LysSer: 5.518 ± 0.759
5.271LysThr: 5.271 ± 0.683
5.766LysVal: 5.766 ± 0.651
1.235LysTrp: 1.235 ± 0.417
3.377LysTyr: 3.377 ± 0.731
0.0LysXaa: 0.0 ± 0.0
Leu
5.518LeuAla: 5.518 ± 0.697
0.494LeuCys: 0.494 ± 0.215
6.672LeuAsp: 6.672 ± 0.653
7.248LeuGlu: 7.248 ± 0.863
2.636LeuPhe: 2.636 ± 0.561
5.024LeuGly: 5.024 ± 0.673
0.824LeuHis: 0.824 ± 0.248
4.612LeuIle: 4.612 ± 0.611
8.484LeuLys: 8.484 ± 0.863
7.989LeuLeu: 7.989 ± 0.937
2.142LeuMet: 2.142 ± 0.396
5.271LeuAsn: 5.271 ± 0.73
2.553LeuPro: 2.553 ± 0.441
3.212LeuGln: 3.212 ± 0.522
3.377LeuArg: 3.377 ± 0.513
7.66LeuSer: 7.66 ± 0.755
5.024LeuThr: 5.024 ± 0.628
5.107LeuVal: 5.107 ± 0.649
0.824LeuTrp: 0.824 ± 0.229
2.471LeuTyr: 2.471 ± 0.402
0.0LeuXaa: 0.0 ± 0.0
Met
1.235MetAla: 1.235 ± 0.317
0.0MetCys: 0.0 ± 0.0
1.565MetAsp: 1.565 ± 0.374
1.565MetGlu: 1.565 ± 0.32
0.659MetPhe: 0.659 ± 0.191
1.483MetGly: 1.483 ± 0.299
0.082MetHis: 0.082 ± 0.078
2.142MetIle: 2.142 ± 0.372
2.306MetLys: 2.306 ± 0.475
1.73MetLeu: 1.73 ± 0.278
0.082MetMet: 0.082 ± 0.089
2.142MetAsn: 2.142 ± 0.399
0.988MetPro: 0.988 ± 0.29
0.659MetGln: 0.659 ± 0.222
1.318MetArg: 1.318 ± 0.352
2.059MetSer: 2.059 ± 0.439
1.071MetThr: 1.071 ± 0.309
1.318MetVal: 1.318 ± 0.229
0.165MetTrp: 0.165 ± 0.098
0.741MetTyr: 0.741 ± 0.264
0.0MetXaa: 0.0 ± 0.0
Asn
3.212AsnAla: 3.212 ± 0.565
0.412AsnCys: 0.412 ± 0.198
3.624AsnAsp: 3.624 ± 0.475
4.777AsnGlu: 4.777 ± 0.547
1.812AsnPhe: 1.812 ± 0.406
3.954AsnGly: 3.954 ± 0.64
0.824AsnHis: 0.824 ± 0.247
4.53AsnIle: 4.53 ± 0.589
5.848AsnLys: 5.848 ± 0.799
4.942AsnLeu: 4.942 ± 0.616
1.235AsnMet: 1.235 ± 0.238
3.13AsnAsn: 3.13 ± 0.622
2.471AsnPro: 2.471 ± 0.447
2.8AsnGln: 2.8 ± 0.411
2.636AsnArg: 2.636 ± 0.371
3.624AsnSer: 3.624 ± 0.419
2.718AsnThr: 2.718 ± 0.464
3.789AsnVal: 3.789 ± 0.507
0.824AsnTrp: 0.824 ± 0.24
2.142AsnTyr: 2.142 ± 0.335
0.0AsnXaa: 0.0 ± 0.0
Pro
2.059ProAla: 2.059 ± 0.484
0.165ProCys: 0.165 ± 0.11
1.977ProAsp: 1.977 ± 0.469
3.212ProGlu: 3.212 ± 0.531
2.389ProPhe: 2.389 ± 0.371
1.318ProGly: 1.318 ± 0.239
0.494ProHis: 0.494 ± 0.203
1.483ProIle: 1.483 ± 0.298
3.212ProLys: 3.212 ± 0.521
2.306ProLeu: 2.306 ± 0.358
0.494ProMet: 0.494 ± 0.21
2.142ProAsn: 2.142 ± 0.429
0.988ProPro: 0.988 ± 0.359
0.988ProGln: 0.988 ± 0.342
1.235ProArg: 1.235 ± 0.25
2.306ProSer: 2.306 ± 0.427
1.071ProThr: 1.071 ± 0.366
1.4ProVal: 1.4 ± 0.394
0.247ProTrp: 0.247 ± 0.117
0.659ProTyr: 0.659 ± 0.239
0.0ProXaa: 0.0 ± 0.0
Gln
4.036GlnAla: 4.036 ± 0.433
0.082GlnCys: 0.082 ± 0.1
1.894GlnAsp: 1.894 ± 0.364
2.553GlnGlu: 2.553 ± 0.53
1.565GlnPhe: 1.565 ± 0.269
2.142GlnGly: 2.142 ± 0.467
0.577GlnHis: 0.577 ± 0.214
2.965GlnIle: 2.965 ± 0.386
2.718GlnLys: 2.718 ± 0.563
3.624GlnLeu: 3.624 ± 0.495
0.824GlnMet: 0.824 ± 0.261
2.142GlnAsn: 2.142 ± 0.444
1.071GlnPro: 1.071 ± 0.269
1.318GlnGln: 1.318 ± 0.384
1.647GlnArg: 1.647 ± 0.365
2.224GlnSer: 2.224 ± 0.36
2.059GlnThr: 2.059 ± 0.432
2.389GlnVal: 2.389 ± 0.447
0.494GlnTrp: 0.494 ± 0.201
1.73GlnTyr: 1.73 ± 0.536
0.0GlnXaa: 0.0 ± 0.0
Arg
2.553ArgAla: 2.553 ± 0.414
0.165ArgCys: 0.165 ± 0.112
2.471ArgAsp: 2.471 ± 0.554
2.306ArgGlu: 2.306 ± 0.427
1.153ArgPhe: 1.153 ± 0.258
2.965ArgGly: 2.965 ± 0.566
0.494ArgHis: 0.494 ± 0.265
3.459ArgIle: 3.459 ± 0.493
4.365ArgLys: 4.365 ± 0.685
4.036ArgLeu: 4.036 ± 0.566
1.071ArgMet: 1.071 ± 0.236
2.224ArgAsn: 2.224 ± 0.441
0.659ArgPro: 0.659 ± 0.176
2.471ArgGln: 2.471 ± 0.418
1.894ArgArg: 1.894 ± 0.355
1.894ArgSer: 1.894 ± 0.54
1.4ArgThr: 1.4 ± 0.337
2.306ArgVal: 2.306 ± 0.419
0.659ArgTrp: 0.659 ± 0.205
1.4ArgTyr: 1.4 ± 0.342
0.0ArgXaa: 0.0 ± 0.0
Ser
3.542SerAla: 3.542 ± 0.437
0.659SerCys: 0.659 ± 0.242
4.777SerAsp: 4.777 ± 0.773
4.777SerGlu: 4.777 ± 0.563
2.142SerPhe: 2.142 ± 0.469
4.695SerGly: 4.695 ± 0.698
0.988SerHis: 0.988 ± 0.29
5.271SerIle: 5.271 ± 0.606
6.095SerLys: 6.095 ± 0.727
5.601SerLeu: 5.601 ± 0.681
1.73SerMet: 1.73 ± 0.36
4.53SerAsn: 4.53 ± 0.585
1.483SerPro: 1.483 ± 0.302
2.224SerGln: 2.224 ± 0.41
2.389SerArg: 2.389 ± 0.447
5.93SerSer: 5.93 ± 1.013
2.718SerThr: 2.718 ± 0.646
3.706SerVal: 3.706 ± 0.543
0.906SerTrp: 0.906 ± 0.226
2.553SerTyr: 2.553 ± 0.458
0.0SerXaa: 0.0 ± 0.0
Thr
3.954ThrAla: 3.954 ± 0.671
0.165ThrCys: 0.165 ± 0.111
3.459ThrAsp: 3.459 ± 0.61
3.789ThrGlu: 3.789 ± 0.525
2.636ThrPhe: 2.636 ± 0.414
4.86ThrGly: 4.86 ± 0.838
0.577ThrHis: 0.577 ± 0.221
3.789ThrIle: 3.789 ± 0.692
5.107ThrLys: 5.107 ± 0.57
3.295ThrLeu: 3.295 ± 0.414
0.824ThrMet: 0.824 ± 0.263
2.636ThrAsn: 2.636 ± 0.512
1.977ThrPro: 1.977 ± 0.458
1.235ThrGln: 1.235 ± 0.301
1.483ThrArg: 1.483 ± 0.429
3.542ThrSer: 3.542 ± 0.553
2.471ThrThr: 2.471 ± 0.472
2.636ThrVal: 2.636 ± 0.359
0.824ThrTrp: 0.824 ± 0.194
2.636ThrTyr: 2.636 ± 0.4
0.0ThrXaa: 0.0 ± 0.0
Val
3.706ValAla: 3.706 ± 0.613
0.247ValCys: 0.247 ± 0.136
4.283ValAsp: 4.283 ± 0.823
3.954ValGlu: 3.954 ± 0.603
2.059ValPhe: 2.059 ± 0.448
3.13ValGly: 3.13 ± 0.499
0.824ValHis: 0.824 ± 0.266
5.189ValIle: 5.189 ± 0.695
6.177ValLys: 6.177 ± 0.646
4.942ValLeu: 4.942 ± 0.811
1.071ValMet: 1.071 ± 0.305
4.283ValAsn: 4.283 ± 0.781
1.812ValPro: 1.812 ± 0.417
2.636ValGln: 2.636 ± 0.426
3.048ValArg: 3.048 ± 0.575
3.871ValSer: 3.871 ± 0.647
3.212ValThr: 3.212 ± 0.574
4.365ValVal: 4.365 ± 0.601
0.494ValTrp: 0.494 ± 0.188
2.553ValTyr: 2.553 ± 0.483
0.0ValXaa: 0.0 ± 0.0
Trp
1.071TrpAla: 1.071 ± 0.281
0.082TrpCys: 0.082 ± 0.078
0.741TrpAsp: 0.741 ± 0.209
1.153TrpGlu: 1.153 ± 0.363
0.577TrpPhe: 0.577 ± 0.181
0.329TrpGly: 0.329 ± 0.17
0.329TrpHis: 0.329 ± 0.161
0.824TrpIle: 0.824 ± 0.267
1.4TrpLys: 1.4 ± 0.289
1.483TrpLeu: 1.483 ± 0.297
0.494TrpMet: 0.494 ± 0.172
1.153TrpAsn: 1.153 ± 0.4
0.165TrpPro: 0.165 ± 0.111
0.494TrpGln: 0.494 ± 0.213
0.577TrpArg: 0.577 ± 0.164
0.659TrpSer: 0.659 ± 0.227
0.412TrpThr: 0.412 ± 0.164
0.659TrpVal: 0.659 ± 0.21
0.0TrpTrp: 0.0 ± 0.0
0.165TrpTyr: 0.165 ± 0.111
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.142TyrAla: 2.142 ± 0.534
0.247TyrCys: 0.247 ± 0.131
1.812TyrAsp: 1.812 ± 0.358
2.8TyrGlu: 2.8 ± 0.439
1.4TyrPhe: 1.4 ± 0.332
2.306TyrGly: 2.306 ± 0.518
0.577TyrHis: 0.577 ± 0.234
2.059TyrIle: 2.059 ± 0.486
2.965TyrLys: 2.965 ± 0.47
2.718TyrLeu: 2.718 ± 0.523
1.071TyrMet: 1.071 ± 0.338
1.812TyrAsn: 1.812 ± 0.403
1.977TyrPro: 1.977 ± 0.468
1.977TyrGln: 1.977 ± 0.47
2.142TyrArg: 2.142 ± 0.418
3.789TyrSer: 3.789 ± 0.55
1.812TyrThr: 1.812 ± 0.468
2.553TyrVal: 2.553 ± 0.579
0.577TyrTrp: 0.577 ± 0.201
1.153TyrTyr: 1.153 ± 0.368
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 66 proteins (12142 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski