Torque teno sus virus k2 (isolate Pig/New Zealand/38E05/2007) (TTSuVk2)
Average proteome isoelectric point is 8.17
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|K4I6Y0|K4I6Y0_TTVK2 Capsid protein OS=Torque teno sus virus k2 (isolate Pig/New Zealand/38E05/2007) OX=1239832 PE=3 SV=1
MM1 pKa = 7.85 GDD3 pKa = 3.55 VQPDD7 pKa = 2.85 LHH9 pKa = 6.45 YY10 pKa = 10.63 RR11 pKa = 11.84 PRR13 pKa = 11.84 TPGRR17 pKa = 11.84 ITRR20 pKa = 11.84 WLDD23 pKa = 2.89 ICRR26 pKa = 11.84 LSHH29 pKa = 7.47 HH30 pKa = 6.76 LFCPCKK36 pKa = 9.12 NWKK39 pKa = 9.33 GHH41 pKa = 5.76 LQLCLRR47 pKa = 11.84 SEE49 pKa = 4.4 DD50 pKa = 3.77 VAGDD54 pKa = 3.48 GGDD57 pKa = 3.47 THH59 pKa = 6.5 ATAVAEE65 pKa = 4.01 FDD67 pKa = 3.46 LGGIEE72 pKa = 4.9 DD73 pKa = 5.65 LIAAAEE79 pKa = 4.04 RR80 pKa = 3.79
Molecular weight: 8.92 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.642
IPC2_protein 5.588
IPC_protein 5.588
Toseland 6.008
ProMoST 5.97
Dawson 5.881
Bjellqvist 5.868
Wikipedia 5.906
Rodwell 5.868
Grimsley 6.148
Solomon 5.881
Lehninger 5.881
Nozaki 6.16
DTASelect 6.351
Thurlkill 6.364
EMBOSS 6.326
Sillero 6.262
Patrickios 3.999
IPC_peptide 5.906
IPC2_peptide 6.287
IPC2.peptide.svr19 6.208
Protein with the highest isoelectric point:
>tr|K4I3V1|K4I3V1_TTVK2 ORF3 OS=Torque teno sus virus k2 (isolate Pig/New Zealand/38E05/2007) OX=1239832 PE=4 SV=1
MM1 pKa = 7.58 PPFRR5 pKa = 11.84 RR6 pKa = 11.84 RR7 pKa = 11.84 RR8 pKa = 11.84 WRR10 pKa = 11.84 WRR12 pKa = 11.84 RR13 pKa = 11.84 YY14 pKa = 7.71 PRR16 pKa = 11.84 YY17 pKa = 8.22 GRR19 pKa = 11.84 RR20 pKa = 11.84 RR21 pKa = 11.84 VRR23 pKa = 11.84 PRR25 pKa = 11.84 RR26 pKa = 11.84 YY27 pKa = 8.13 RR28 pKa = 11.84 RR29 pKa = 11.84 SYY31 pKa = 10.15 RR32 pKa = 11.84 RR33 pKa = 11.84 GRR35 pKa = 11.84 KK36 pKa = 8.13 WGGHH40 pKa = 4.03 GEE42 pKa = 4.18 EE43 pKa = 4.56 RR44 pKa = 11.84 FEE46 pKa = 4.49 TAIGDD51 pKa = 4.19 PKK53 pKa = 10.57 TIPCPDD59 pKa = 3.09 IFNPGTGTFRR69 pKa = 11.84 TWVRR73 pKa = 11.84 NPLEE77 pKa = 3.91 VHH79 pKa = 6.58 KK80 pKa = 11.07 GILQPWEE87 pKa = 3.77 YY88 pKa = 10.86 DD89 pKa = 3.13 YY90 pKa = 11.9 DD91 pKa = 4.05 GLVRR95 pKa = 11.84 TDD97 pKa = 3.16 TLRR100 pKa = 11.84 RR101 pKa = 11.84 MLQYY105 pKa = 9.76 STEE108 pKa = 3.99 EE109 pKa = 3.96 DD110 pKa = 3.24 EE111 pKa = 4.74 TRR113 pKa = 11.84 EE114 pKa = 3.59 KK115 pKa = 10.76 AAPFRR120 pKa = 11.84 GLGRR124 pKa = 11.84 GKK126 pKa = 10.97 GPFSDD131 pKa = 3.94 TSDD134 pKa = 3.7 GSDD137 pKa = 3.05 TSSSEE142 pKa = 3.93 SFDD145 pKa = 3.39 YY146 pKa = 11.37 CEE148 pKa = 5.09 EE149 pKa = 4.04 EE150 pKa = 5.48 KK151 pKa = 10.76 EE152 pKa = 4.05 DD153 pKa = 3.73 QAAEE157 pKa = 4.13 EE158 pKa = 4.33 ARR160 pKa = 11.84 QEE162 pKa = 4.09 HH163 pKa = 5.9 QLRR166 pKa = 11.84 LLNKK170 pKa = 9.4 RR171 pKa = 11.84 RR172 pKa = 11.84 KK173 pKa = 9.24 LKK175 pKa = 10.36 QLFRR179 pKa = 11.84 FLKK182 pKa = 10.37 RR183 pKa = 11.84 IKK185 pKa = 9.25 TCRR188 pKa = 3.27
Molecular weight: 22.78 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.342
IPC2_protein 9.575
IPC_protein 10.423
Toseland 10.511
ProMoST 10.35
Dawson 10.643
Bjellqvist 10.365
Wikipedia 10.862
Rodwell 10.774
Grimsley 10.701
Solomon 10.745
Lehninger 10.701
Nozaki 10.496
DTASelect 10.365
Thurlkill 10.526
EMBOSS 10.906
Sillero 10.57
Patrickios 10.438
IPC_peptide 10.745
IPC2_peptide 9.385
IPC2.peptide.svr19 8.663
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4
0
4
1121
80
629
280.3
33.09
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
4.817 ± 1.014
1.963 ± 0.489
6.244 ± 0.561
6.155 ± 0.785
4.46 ± 0.464
6.869 ± 0.743
2.676 ± 0.646
3.479 ± 0.247
5.531 ± 0.476
8.564 ± 0.716
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.963 ± 0.29
3.747 ± 1.252
6.066 ± 0.451
4.014 ± 0.584
12.31 ± 1.526
5.531 ± 1.175
6.066 ± 0.451
2.765 ± 0.173
3.033 ± 0.752
3.747 ± 0.936
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here