Sowbane mosaic virus

Taxonomy: Viruses; Riboviria; Orthornavirae; Pisuviricota; Pisoniviricetes; Sobelivirales; Solemoviridae; Sobemovirus

Average proteome isoelectric point is 7.12

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|B5DC59|B5DC59_9VIRU Capsid protein OS=Sowbane mosaic virus OX=378833 GN=CP PE=3 SV=1
MM1 pKa = 7.15HH2 pKa = 7.85RR3 pKa = 11.84SPFGYY8 pKa = 10.5LLLLISLPAGAVTYY22 pKa = 10.62DD23 pKa = 4.78CIRR26 pKa = 11.84GDD28 pKa = 3.99CQAGPNSLMMALMTLLTTGMWWVVSLSLFWIEE60 pKa = 3.78RR61 pKa = 11.84QRR63 pKa = 11.84YY64 pKa = 8.18RR65 pKa = 11.84SAEE68 pKa = 4.05EE69 pKa = 4.11KK70 pKa = 10.04ISRR73 pKa = 11.84PKK75 pKa = 10.3RR76 pKa = 11.84LRR78 pKa = 11.84LIGDD82 pKa = 4.53PYY84 pKa = 11.32LDD86 pKa = 4.08PCDD89 pKa = 4.92GIVGKK94 pKa = 10.34ILDD97 pKa = 4.49DD98 pKa = 3.7CTGLVQEE105 pKa = 5.53VVIQPKK111 pKa = 6.53WWRR114 pKa = 11.84FISMSSPTINQDD126 pKa = 2.86EE127 pKa = 4.45DD128 pKa = 3.89NEE130 pKa = 4.35CAILGNSYY138 pKa = 10.45SQVVPRR144 pKa = 11.84SEE146 pKa = 4.12PGSFVLLKK154 pKa = 10.93VNEE157 pKa = 4.28EE158 pKa = 4.29VVGAGCRR165 pKa = 11.84VIYY168 pKa = 10.68DD169 pKa = 3.58GGDD172 pKa = 3.51YY173 pKa = 10.98LLTAHH178 pKa = 6.67HH179 pKa = 6.75VWSQAPNHH187 pKa = 6.11IAKK190 pKa = 10.21GGKK193 pKa = 6.38TVEE196 pKa = 4.37ISMEE200 pKa = 4.06MKK202 pKa = 10.35PYY204 pKa = 10.5LSSKK208 pKa = 10.8NKK210 pKa = 9.77VLDD213 pKa = 3.73FCLVPVPAAVWSNLGVKK230 pKa = 9.69SSKK233 pKa = 9.96IASLHH238 pKa = 4.74QRR240 pKa = 11.84SNVTVYY246 pKa = 10.92GGTASTMLLSSFGIAEE262 pKa = 4.33ADD264 pKa = 3.79DD265 pKa = 3.82NPLRR269 pKa = 11.84IIHH272 pKa = 6.32KK273 pKa = 10.28ASTARR278 pKa = 11.84AWSGSPLYY286 pKa = 10.53NSNGLVLGVHH296 pKa = 6.95LGYY299 pKa = 10.72DD300 pKa = 3.49QLGSTNRR307 pKa = 11.84AVNIGYY313 pKa = 8.56VLRR316 pKa = 11.84TTSSNEE322 pKa = 3.68TAPPDD327 pKa = 3.72LNFVEE332 pKa = 4.52ITEE335 pKa = 4.61DD336 pKa = 3.55EE337 pKa = 4.39AVDD340 pKa = 3.73RR341 pKa = 11.84PSFDD345 pKa = 3.15EE346 pKa = 4.25YY347 pKa = 10.87EE348 pKa = 3.88IEE350 pKa = 4.23GFGKK354 pKa = 9.62IRR356 pKa = 11.84TRR358 pKa = 11.84GRR360 pKa = 11.84EE361 pKa = 3.65YY362 pKa = 10.75YY363 pKa = 9.97IPRR366 pKa = 11.84NKK368 pKa = 10.18DD369 pKa = 2.49WNKK372 pKa = 10.5YY373 pKa = 9.92DD374 pKa = 6.0DD375 pKa = 4.67EE376 pKa = 7.48DD377 pKa = 5.96DD378 pKa = 4.58DD379 pKa = 5.72AFFDD383 pKa = 4.22VPVALWLNSNEE394 pKa = 4.25TVEE397 pKa = 5.68KK398 pKa = 10.31PFKK401 pKa = 9.83LQRR404 pKa = 11.84GRR406 pKa = 11.84KK407 pKa = 6.72FTALAALIEE416 pKa = 4.33LGNYY420 pKa = 7.4SWEE423 pKa = 4.5SGGHH427 pKa = 4.66YY428 pKa = 9.31PQGVGLQFVGRR439 pKa = 11.84STCLFRR445 pKa = 11.84EE446 pKa = 4.49SSRR449 pKa = 11.84KK450 pKa = 9.55ALSEE454 pKa = 3.93RR455 pKa = 11.84FKK457 pKa = 10.93LASEE461 pKa = 4.71TFPEE465 pKa = 4.19LLDD468 pKa = 4.04YY469 pKa = 10.74NWPARR474 pKa = 11.84GSKK477 pKa = 10.8AEE479 pKa = 4.36LGSLLLQAEE488 pKa = 4.87RR489 pKa = 11.84FRR491 pKa = 11.84CTEE494 pKa = 3.73PPEE497 pKa = 4.34NLASCCDD504 pKa = 3.85KK505 pKa = 10.88LASRR509 pKa = 11.84YY510 pKa = 8.47PVTRR514 pKa = 11.84PRR516 pKa = 11.84CCFRR520 pKa = 11.84TDD522 pKa = 2.62KK523 pKa = 10.42WSWSAVEE530 pKa = 4.41EE531 pKa = 4.23EE532 pKa = 4.77VKK534 pKa = 10.69RR535 pKa = 11.84LAAQGRR541 pKa = 11.84EE542 pKa = 4.14VCKK545 pKa = 10.57DD546 pKa = 3.52SSPGSPLASLCKK558 pKa = 10.14RR559 pKa = 11.84NQDD562 pKa = 3.15VLAAHH567 pKa = 7.36LDD569 pKa = 3.96FVVKK573 pKa = 10.49AVTEE577 pKa = 4.12RR578 pKa = 11.84LFLLAEE584 pKa = 4.35TEE586 pKa = 4.18LHH588 pKa = 6.02GLSPVEE594 pKa = 5.59LINQGCCDD602 pKa = 3.88PVRR605 pKa = 11.84LFVKK609 pKa = 10.17QEE611 pKa = 3.45PHH613 pKa = 4.77TLKK616 pKa = 10.65KK617 pKa = 10.33INEE620 pKa = 3.7GRR622 pKa = 11.84YY623 pKa = 9.14RR624 pKa = 11.84LISSVSLVDD633 pKa = 3.76QIVEE637 pKa = 4.03RR638 pKa = 11.84MLFGPQNRR646 pKa = 11.84AEE648 pKa = 3.95IALWDD653 pKa = 4.54TIPSKK658 pKa = 10.8PGMGLTLKK666 pKa = 10.53SQARR670 pKa = 11.84KK671 pKa = 9.82IFGDD675 pKa = 3.67LLVKK679 pKa = 9.39HH680 pKa = 5.69THH682 pKa = 5.85CPAYY686 pKa = 9.85EE687 pKa = 4.0ADD689 pKa = 3.17ISGFDD694 pKa = 3.33WTVQDD699 pKa = 3.38WEE701 pKa = 4.17LWADD705 pKa = 3.26VEE707 pKa = 5.27IRR709 pKa = 11.84IKK711 pKa = 10.79LCSAGVNLAQCMRR724 pKa = 11.84NRR726 pKa = 11.84FYY728 pKa = 11.53CFMNSVFQLSDD739 pKa = 2.97GTLIQQCSPGVMKK752 pKa = 10.69SGSYY756 pKa = 8.6CTSSTNSRR764 pKa = 11.84VRR766 pKa = 11.84CLMAEE771 pKa = 4.45IIGSPWCIAMGDD783 pKa = 3.95DD784 pKa = 3.6SVEE787 pKa = 4.34GYY789 pKa = 10.66VEE791 pKa = 4.33GARR794 pKa = 11.84DD795 pKa = 3.35KK796 pKa = 11.56YY797 pKa = 11.08DD798 pKa = 3.89ALGHH802 pKa = 5.92KK803 pKa = 10.31CKK805 pKa = 10.39DD806 pKa = 3.66YY807 pKa = 10.96QVCDD811 pKa = 3.26SDD813 pKa = 4.74GLLLRR818 pKa = 11.84SVGFCSHH825 pKa = 7.18HH826 pKa = 6.66IDD828 pKa = 3.87SSGAYY833 pKa = 6.7LTSWAKK839 pKa = 9.3TLFKK843 pKa = 10.47HH844 pKa = 6.31LHH846 pKa = 6.02SKK848 pKa = 11.15DD849 pKa = 3.6EE850 pKa = 4.36DD851 pKa = 3.8FQDD854 pKa = 4.14IEE856 pKa = 4.2IEE858 pKa = 4.18LRR860 pKa = 11.84NSPMWPSIRR869 pKa = 11.84RR870 pKa = 11.84YY871 pKa = 9.38LVQEE875 pKa = 4.19TPSLDD880 pKa = 3.19KK881 pKa = 10.67RR882 pKa = 11.84YY883 pKa = 10.01EE884 pKa = 4.0PNEE887 pKa = 3.92EE888 pKa = 4.27EE889 pKa = 4.16EE890 pKa = 4.48VQTTEE895 pKa = 4.0QAVKK899 pKa = 9.28STAQQVHH906 pKa = 6.05GASCFTTCCEE916 pKa = 4.13RR917 pKa = 11.84NCYY920 pKa = 8.45GQGYY924 pKa = 10.29ACVQWW929 pKa = 3.84

Molecular weight: 104.21 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|B5DC59|B5DC59_9VIRU Capsid protein OS=Sowbane mosaic virus OX=378833 GN=CP PE=3 SV=1
MM1 pKa = 7.13NQTKK5 pKa = 10.05KK6 pKa = 10.59KK7 pKa = 10.02KK8 pKa = 9.73SRR10 pKa = 11.84PRR12 pKa = 11.84NKK14 pKa = 9.61QSKK17 pKa = 8.31AQPNKK22 pKa = 9.06STAQVVSQPAAKK34 pKa = 9.89GIVMGRR40 pKa = 11.84AMPVFNGKK48 pKa = 10.15AGVLRR53 pKa = 11.84LRR55 pKa = 11.84HH56 pKa = 5.99HH57 pKa = 7.35EE58 pKa = 3.83ILQTIVSTTDD68 pKa = 2.54KK69 pKa = 9.96YY70 pKa = 8.9TVRR73 pKa = 11.84SVNLVPANFSWLSGVAVNFSKK94 pKa = 10.52WKK96 pKa = 7.86WHH98 pKa = 5.87ALNLVYY104 pKa = 10.73VPACSTSVQGNIDD117 pKa = 3.3MALQYY122 pKa = 10.87DD123 pKa = 4.4GNDD126 pKa = 3.29IPATTPEE133 pKa = 4.62EE134 pKa = 3.91LSVMYY139 pKa = 9.52GHH141 pKa = 6.74SGGPVWSGTAGCRR154 pKa = 11.84LLDD157 pKa = 3.81NATMSGRR164 pKa = 11.84GKK166 pKa = 9.7PPEE169 pKa = 4.9AIATIVDD176 pKa = 3.25VSKK179 pKa = 10.77FSKK182 pKa = 10.65SNYY185 pKa = 8.97LYY187 pKa = 10.74RR188 pKa = 11.84SGALSGDD195 pKa = 3.68SQTIYY200 pKa = 10.62CPCDD204 pKa = 3.21LQYY207 pKa = 11.51GVTAGITPTVGAIGYY222 pKa = 8.53IWATYY227 pKa = 8.91DD228 pKa = 3.24VEE230 pKa = 4.37MFEE233 pKa = 4.69PLPARR238 pKa = 11.84LNKK241 pKa = 10.21

Molecular weight: 26.16 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4

0

4

1886

164

929

471.5

52.27

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.787 ± 0.401

3.022 ± 0.809

5.143 ± 0.623

5.62 ± 0.818

3.128 ± 0.439

7.052 ± 0.471

1.856 ± 0.158

4.613 ± 0.103

5.355 ± 0.366

8.961 ± 1.114

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.803 ± 0.252

4.083 ± 0.319

5.514 ± 0.476

3.712 ± 0.176

5.514 ± 0.372

9.544 ± 0.516

5.143 ± 0.552

7.635 ± 0.797

2.121 ± 0.15

3.393 ± 0.174
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski