Beihai victori-like virus 1
Average proteome isoelectric point is 7.53
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1L3KFD2|A0A1L3KFD2_9VIRU Uncharacterized protein OS=Beihai victori-like virus 1 OX=1922737 PE=4 SV=1
MM1 pKa = 7.68 RR2 pKa = 11.84 EE3 pKa = 3.79 IVTRR7 pKa = 11.84 AATEE11 pKa = 4.24 LYY13 pKa = 10.48 RR14 pKa = 11.84 NVEE17 pKa = 3.95 RR18 pKa = 11.84 AFNPVNHH25 pKa = 6.54 SLSEE29 pKa = 4.04 VDD31 pKa = 3.72 LGRR34 pKa = 11.84 VDD36 pKa = 5.42 FVTVPPHH43 pKa = 5.96 NNNIRR48 pKa = 11.84 TRR50 pKa = 11.84 AGDD53 pKa = 3.59 FEE55 pKa = 5.45 VNTTASGARR64 pKa = 11.84 TLEE67 pKa = 3.94 ATSAQRR73 pKa = 11.84 SPIDD77 pKa = 3.66 LLAEE81 pKa = 4.44 GLQRR85 pKa = 11.84 RR86 pKa = 11.84 SVEE89 pKa = 3.83 LARR92 pKa = 11.84 EE93 pKa = 4.05 RR94 pKa = 11.84 NSIQVNSQRR103 pKa = 11.84 VKK105 pKa = 9.17 NRR107 pKa = 11.84 AVGGEE112 pKa = 3.68 YY113 pKa = 8.06 TQRR116 pKa = 11.84 VRR118 pKa = 11.84 IDD120 pKa = 2.97 TGEE123 pKa = 4.17 TYY125 pKa = 10.81 EE126 pKa = 4.22 EE127 pKa = 4.08 LQQEE131 pKa = 4.47 SEE133 pKa = 4.2 EE134 pKa = 4.06 FRR136 pKa = 11.84 TNRR139 pKa = 11.84 FNTVEE144 pKa = 3.72 NQLRR148 pKa = 11.84 DD149 pKa = 3.6 KK150 pKa = 10.35 YY151 pKa = 11.38 GKK153 pKa = 9.5 IAFSGGTFTVNQTDD167 pKa = 4.16 LGALSPGNLLDD178 pKa = 4.44 HH179 pKa = 6.68 GVPYY183 pKa = 10.18 HH184 pKa = 7.13 DD185 pKa = 4.22 KK186 pKa = 10.87 AYY188 pKa = 10.58 VSKK191 pKa = 9.08 FTPFGYY197 pKa = 10.18 KK198 pKa = 9.85 RR199 pKa = 11.84 FEE201 pKa = 4.38 LDD203 pKa = 2.75 IGPTTAHH210 pKa = 6.77 ASAVKK215 pKa = 9.91 QCLFVKK221 pKa = 9.8 TDD223 pKa = 3.19 EE224 pKa = 5.45 LIPHH228 pKa = 7.38 KK229 pKa = 10.24 IKK231 pKa = 10.75 EE232 pKa = 4.23 LIGRR236 pKa = 11.84 VNYY239 pKa = 10.82 ANGVNNTEE247 pKa = 4.05 LRR249 pKa = 11.84 QFLYY253 pKa = 10.68 VRR255 pKa = 11.84 RR256 pKa = 11.84 FHH258 pKa = 6.57 QNNPIDD264 pKa = 3.71 ISSGSIRR271 pKa = 11.84 IYY273 pKa = 11.08 DD274 pKa = 3.49 NWKK277 pKa = 9.84 EE278 pKa = 3.74 LYY280 pKa = 9.73 EE281 pKa = 4.44 AIKK284 pKa = 10.31 AHH286 pKa = 6.07 EE287 pKa = 4.12 VSKK290 pKa = 11.07 LNSISDD296 pKa = 3.65 RR297 pKa = 11.84 LRR299 pKa = 11.84 NSGAEE304 pKa = 3.69 IAVRR308 pKa = 11.84 QAIAEE313 pKa = 4.06 GDD315 pKa = 3.64 LFFNNTQYY323 pKa = 11.37 SRR325 pKa = 11.84 RR326 pKa = 11.84 NLIKK330 pKa = 10.38 AWAKK334 pKa = 10.45 HH335 pKa = 3.79 YY336 pKa = 9.4 TGLNSYY342 pKa = 10.14 SYY344 pKa = 11.03 QRR346 pKa = 11.84 TLQVVGGLLLLFTAYY361 pKa = 10.93 SLVFRR366 pKa = 11.84 YY367 pKa = 7.69 ATLGYY372 pKa = 9.56 KK373 pKa = 9.9 QYY375 pKa = 9.78 TLSEE379 pKa = 4.02 LWEE382 pKa = 4.36 LDD384 pKa = 2.85 SWTKK388 pKa = 10.26 YY389 pKa = 9.12 PSIFIRR395 pKa = 11.84 YY396 pKa = 8.49 LPIFASDD403 pKa = 3.34 EE404 pKa = 4.37 AIKK407 pKa = 10.54 RR408 pKa = 11.84 EE409 pKa = 3.83 RR410 pKa = 11.84 RR411 pKa = 11.84 EE412 pKa = 3.39 RR413 pKa = 11.84 RR414 pKa = 11.84 QRR416 pKa = 11.84 EE417 pKa = 3.81 RR418 pKa = 11.84 ARR420 pKa = 11.84 EE421 pKa = 4.01 LISWVGEE428 pKa = 3.6 GRR430 pKa = 11.84 LNIVRR435 pKa = 11.84 SFIEE439 pKa = 4.35 SYY441 pKa = 9.83 HH442 pKa = 7.4 AEE444 pKa = 3.93 WLLAGSPEE452 pKa = 4.36 TNSLDD457 pKa = 3.69 LEE459 pKa = 4.19 ALILSVGQEE468 pKa = 3.37 IGFDD472 pKa = 3.69 FTRR475 pKa = 11.84 SRR477 pKa = 11.84 TLLIDD482 pKa = 3.55 LLEE485 pKa = 5.41 RR486 pKa = 11.84 EE487 pKa = 4.43 EE488 pKa = 4.4 VPRR491 pKa = 11.84 LPQIFEE497 pKa = 4.69 HH498 pKa = 6.55 NVLNQLLPAVIAAEE512 pKa = 3.95 AHH514 pKa = 5.49
Molecular weight: 58.99 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 7.468
IPC2_protein 7.746
IPC_protein 7.936
Toseland 8.214
ProMoST 8.097
Dawson 8.507
Bjellqvist 8.361
Wikipedia 8.639
Rodwell 8.521
Grimsley 8.375
Solomon 8.726
Lehninger 8.668
Nozaki 8.302
DTASelect 8.404
Thurlkill 8.419
EMBOSS 8.682
Sillero 8.58
Patrickios 4.52
IPC_peptide 8.697
IPC2_peptide 7.395
IPC2.peptide.svr19 7.549
Protein with the highest isoelectric point:
>tr|A0A1L3KFD2|A0A1L3KFD2_9VIRU Uncharacterized protein OS=Beihai victori-like virus 1 OX=1922737 PE=4 SV=1
MM1 pKa = 7.69 SSTSYY6 pKa = 10.85 YY7 pKa = 10.04 RR8 pKa = 11.84 QSLQQKK14 pKa = 7.82 LTNDD18 pKa = 2.94 SQLRR22 pKa = 11.84 DD23 pKa = 4.09 RR24 pKa = 11.84 IDD26 pKa = 5.81 DD27 pKa = 3.54 ILTTNARR34 pKa = 11.84 KK35 pKa = 9.64 PEE37 pKa = 4.19 DD38 pKa = 3.68 FEE40 pKa = 4.77 IAIQALDD47 pKa = 3.85 FLCIDD52 pKa = 4.27 VITFLSLVDD61 pKa = 3.72 IPLARR66 pKa = 11.84 IDD68 pKa = 3.79 RR69 pKa = 11.84 LHH71 pKa = 6.51 EE72 pKa = 4.18 RR73 pKa = 11.84 MEE75 pKa = 4.57 ALGSTSSFLLRR86 pKa = 11.84 HH87 pKa = 6.34 LKK89 pKa = 10.51 PSDD92 pKa = 3.28 FVDD95 pKa = 3.75 RR96 pKa = 11.84 FTLRR100 pKa = 11.84 DD101 pKa = 3.55 DD102 pKa = 4.42 AKK104 pKa = 10.61 HH105 pKa = 6.3 LKK107 pKa = 10.05 YY108 pKa = 10.81 LEE110 pKa = 4.2 NRR112 pKa = 11.84 DD113 pKa = 3.39 TVRR116 pKa = 11.84 AVAYY120 pKa = 10.08 SIMIMEE126 pKa = 5.63 FPMQLKK132 pKa = 10.27 PSTNTVLTVLDD143 pKa = 4.0 AVVDD147 pKa = 3.92 TRR149 pKa = 11.84 WIDD152 pKa = 3.31 KK153 pKa = 10.56 LPNHH157 pKa = 6.73 SDD159 pKa = 3.09 IQIDD163 pKa = 3.55 SAAWRR168 pKa = 11.84 KK169 pKa = 6.34 TTFPTKK175 pKa = 10.11 KK176 pKa = 9.95 HH177 pKa = 5.28 VGATNKK183 pKa = 10.44 ANVFLSEE190 pKa = 4.02 IMRR193 pKa = 11.84 DD194 pKa = 3.27 LEE196 pKa = 4.44 AFNKK200 pKa = 8.2 ATWRR204 pKa = 11.84 FFIGYY209 pKa = 7.76 GVPKK213 pKa = 9.3 MASHH217 pKa = 6.8 SLMSEE222 pKa = 3.84 DD223 pKa = 5.06 RR224 pKa = 11.84 LSDD227 pKa = 3.72 DD228 pKa = 3.51 QVCAIVMATVALTEE242 pKa = 3.96 VLGNSLRR249 pKa = 11.84 AFKK252 pKa = 10.44 IAFTIADD259 pKa = 5.28 DD260 pKa = 3.57 IDD262 pKa = 3.9 TANKK266 pKa = 8.19 LTTVLKK272 pKa = 10.89 SLGQNGCRR280 pKa = 11.84 TGAMLVEE287 pKa = 4.75 GKK289 pKa = 9.72 CLKK292 pKa = 10.69 GRR294 pKa = 11.84 GLPSKK299 pKa = 10.11 MATDD303 pKa = 3.57 WKK305 pKa = 10.68 YY306 pKa = 9.78 RR307 pKa = 11.84 TNRR310 pKa = 11.84 TQALSKK316 pKa = 7.54 THH318 pKa = 6.5 KK319 pKa = 10.64 KK320 pKa = 10.15 VDD322 pKa = 3.54 PDD324 pKa = 3.49 KK325 pKa = 11.19 LRR327 pKa = 11.84 TAIRR331 pKa = 11.84 YY332 pKa = 8.93 VYY334 pKa = 10.05 EE335 pKa = 4.35 KK336 pKa = 9.94 EE337 pKa = 4.11 LPRR340 pKa = 11.84 TAVDD344 pKa = 3.16 RR345 pKa = 11.84 VVYY348 pKa = 10.55 EE349 pKa = 4.96 NFDD352 pKa = 3.73 DD353 pKa = 3.49 WWEE356 pKa = 4.16 TRR358 pKa = 11.84 VQWCVNGSHH367 pKa = 6.54 SNRR370 pKa = 11.84 FSRR373 pKa = 11.84 IYY375 pKa = 10.58 KK376 pKa = 9.14 HH377 pKa = 6.39 LKK379 pKa = 8.97 VDD381 pKa = 3.77 SAVLKK386 pKa = 10.74 AEE388 pKa = 3.91 KK389 pKa = 9.56 ATIFRR394 pKa = 11.84 KK395 pKa = 10.21 VFVEE399 pKa = 4.61 HH400 pKa = 6.41 CQKK403 pKa = 10.86 EE404 pKa = 4.42 PVSDD408 pKa = 3.52 WPGISYY414 pKa = 9.3 YY415 pKa = 10.91 SPSDD419 pKa = 3.37 KK420 pKa = 11.22 LEE422 pKa = 4.43 AGASRR427 pKa = 11.84 AIYY430 pKa = 10.56 SCDD433 pKa = 2.97 TLTYY437 pKa = 10.64 LAFEE441 pKa = 4.77 HH442 pKa = 6.54 LLKK445 pKa = 10.56 PVEE448 pKa = 4.2 RR449 pKa = 11.84 CWKK452 pKa = 8.34 NEE454 pKa = 3.62 KK455 pKa = 10.62 VILDD459 pKa = 3.9 PGNDD463 pKa = 3.2 GTYY466 pKa = 10.93 GMNRR470 pKa = 11.84 RR471 pKa = 11.84 IRR473 pKa = 11.84 RR474 pKa = 11.84 LRR476 pKa = 11.84 SSGGRR481 pKa = 11.84 FDD483 pKa = 3.98 VNIMLDD489 pKa = 3.45 YY490 pKa = 11.45 DD491 pKa = 4.55 DD492 pKa = 6.4 FNSCHH497 pKa = 6.09 TNEE500 pKa = 4.35 FMSIVIDD507 pKa = 3.71 EE508 pKa = 4.45 LVKK511 pKa = 10.32 HH512 pKa = 5.8 VSYY515 pKa = 11.05 AEE517 pKa = 3.46 QMYY520 pKa = 10.41 AHH522 pKa = 7.38 GYY524 pKa = 10.2 DD525 pKa = 3.7 GEE527 pKa = 4.45 LLCRR531 pKa = 11.84 RR532 pKa = 11.84 LVEE535 pKa = 3.87 SLYY538 pKa = 9.81 RR539 pKa = 11.84 GRR541 pKa = 11.84 MFVPNSRR548 pKa = 11.84 NPDD551 pKa = 3.37 NPEE554 pKa = 3.77 TKK556 pKa = 9.68 QVYY559 pKa = 7.3 ATLMSGHH566 pKa = 7.16 RR567 pKa = 11.84 ATTFINSVLNYY578 pKa = 10.43 AYY580 pKa = 9.76 IEE582 pKa = 4.04 VAYY585 pKa = 9.68 PGVRR589 pKa = 11.84 NMMSMHH595 pKa = 6.45 VGDD598 pKa = 5.54 DD599 pKa = 3.94 VIVLCRR605 pKa = 11.84 TYY607 pKa = 11.15 EE608 pKa = 3.77 DD609 pKa = 4.4 AYY611 pKa = 11.06 ALLRR615 pKa = 11.84 SCKK618 pKa = 9.28 MLEE621 pKa = 4.32 LNMNPIKK628 pKa = 10.55 QSVGEE633 pKa = 4.08 YY634 pKa = 8.73 VAEE637 pKa = 4.41 FLRR640 pKa = 11.84 VAHH643 pKa = 6.69 VDD645 pKa = 4.05 MICYY649 pKa = 9.89 GYY651 pKa = 10.0 MMRR654 pKa = 11.84 SIGSCVCGNWVNEE667 pKa = 3.18 IRR669 pKa = 11.84 LNYY672 pKa = 10.09 EE673 pKa = 3.27 EE674 pKa = 4.45 GLRR677 pKa = 11.84 TIINHH682 pKa = 5.57 GWTLHH687 pKa = 5.64 NRR689 pKa = 11.84 SHH691 pKa = 5.45 TQFVPLLLVSSMVRR705 pKa = 11.84 MARR708 pKa = 11.84 VSRR711 pKa = 11.84 GNACNLLTGSTGLEE725 pKa = 3.78 PGFQRR730 pKa = 11.84 SRR732 pKa = 11.84 RR733 pKa = 11.84 VGATTLKK740 pKa = 10.66 LKK742 pKa = 10.62 YY743 pKa = 9.96 KK744 pKa = 10.28 QDD746 pKa = 3.58 DD747 pKa = 4.37 RR748 pKa = 11.84 EE749 pKa = 4.53 SQWLTDD755 pKa = 4.19 KK756 pKa = 11.13 VSQCKK761 pKa = 10.02 SLATDD766 pKa = 4.59 DD767 pKa = 4.11 YY768 pKa = 11.37 LSTRR772 pKa = 11.84 VSDD775 pKa = 4.05 LEE777 pKa = 4.39 RR778 pKa = 11.84 EE779 pKa = 4.21 VLEE782 pKa = 5.11 AEE784 pKa = 4.96 GISPKK789 pKa = 10.52 NEE791 pKa = 3.79 LMLSSYY797 pKa = 10.24 KK798 pKa = 10.43 KK799 pKa = 10.54 SFAGSQPYY807 pKa = 10.83 SKK809 pKa = 10.59 FLDD812 pKa = 3.86 AAPKK816 pKa = 9.74 LVRR819 pKa = 11.84 YY820 pKa = 6.3 QHH822 pKa = 6.39 KK823 pKa = 10.18 FKK825 pKa = 11.21 GHH827 pKa = 6.63 IDD829 pKa = 3.72 LDD831 pKa = 4.38 EE832 pKa = 4.55 AWDD835 pKa = 4.39 ARR837 pKa = 11.84 DD838 pKa = 4.02 KK839 pKa = 11.28 LPKK842 pKa = 10.43 GLLTEE847 pKa = 4.17 YY848 pKa = 10.56 PMLVLVRR855 pKa = 11.84 NRR857 pKa = 11.84 IRR859 pKa = 11.84 RR860 pKa = 11.84 STVRR864 pKa = 11.84 VLLFEE869 pKa = 4.27 MTGNLYY875 pKa = 10.41 YY876 pKa = 10.75 GKK878 pKa = 9.93 QLDD881 pKa = 3.74 EE882 pKa = 4.45 VAWGEE887 pKa = 4.27 RR888 pKa = 11.84 EE889 pKa = 4.08 GGTLCSCPLSYY900 pKa = 11.27 SDD902 pKa = 4.5 AGSLAGVCQWKK913 pKa = 7.94 TVYY916 pKa = 10.63 SSFRR920 pKa = 11.84 AYY922 pKa = 9.93 VV923 pKa = 2.99
Molecular weight: 105.64 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 7.586
IPC2_protein 7.922
IPC_protein 7.702
Toseland 7.395
ProMoST 8.317
Dawson 8.478
Bjellqvist 8.785
Wikipedia 8.419
Rodwell 8.507
Grimsley 7.439
Solomon 8.507
Lehninger 8.521
Nozaki 8.916
DTASelect 8.521
Thurlkill 8.58
EMBOSS 8.653
Sillero 8.887
Patrickios 4.342
IPC_peptide 8.507
IPC2_peptide 7.673
IPC2.peptide.svr19 7.923
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
0
2
1437
514
923
718.5
82.31
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.75 ± 0.361
1.461 ± 0.712
5.706 ± 1.02
6.472 ± 1.173
3.827 ± 0.364
5.289 ± 0.198
2.366 ± 0.018
5.01 ± 0.573
5.08 ± 0.887
10.16 ± 0.085
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.157 ± 1.103
4.941 ± 0.722
3.132 ± 0.01
3.132 ± 0.536
8.212 ± 0.633
7.376 ± 0.319
6.054 ± 0.013
7.029 ± 0.342
1.461 ± 0.165
4.384 ± 0.058
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here