Amino acid dipepetide frequency for Mycobacterium phage AltPhacts

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
17.551AlaAla: 17.551 ± 1.353
1.307AlaCys: 1.307 ± 0.285
7.562AlaAsp: 7.562 ± 0.523
7.422AlaGlu: 7.422 ± 0.673
2.334AlaPhe: 2.334 ± 0.352
10.549AlaGly: 10.549 ± 1.153
2.381AlaHis: 2.381 ± 0.34
4.621AlaIle: 4.621 ± 0.556
3.688AlaLys: 3.688 ± 0.447
10.503AlaLeu: 10.503 ± 0.788
3.081AlaMet: 3.081 ± 0.418
3.127AlaAsn: 3.127 ± 0.343
6.908AlaPro: 6.908 ± 0.496
4.201AlaGln: 4.201 ± 0.472
8.589AlaArg: 8.589 ± 0.66
5.648AlaSer: 5.648 ± 0.528
7.749AlaThr: 7.749 ± 0.623
9.056AlaVal: 9.056 ± 0.867
1.961AlaTrp: 1.961 ± 0.304
2.987AlaTyr: 2.987 ± 0.36
0.0AlaXaa: 0.0 ± 0.0
Cys
0.84CysAla: 0.84 ± 0.211
0.0CysCys: 0.0 ± 0.0
1.12CysAsp: 1.12 ± 0.26
0.7CysGlu: 0.7 ± 0.217
0.327CysPhe: 0.327 ± 0.108
1.587CysGly: 1.587 ± 0.286
0.373CysHis: 0.373 ± 0.142
0.327CysIle: 0.327 ± 0.115
0.233CysLys: 0.233 ± 0.1
0.7CysLeu: 0.7 ± 0.215
0.233CysMet: 0.233 ± 0.1
0.327CysAsn: 0.327 ± 0.144
0.934CysPro: 0.934 ± 0.231
0.233CysGln: 0.233 ± 0.095
0.934CysArg: 0.934 ± 0.259
0.467CysSer: 0.467 ± 0.137
0.56CysThr: 0.56 ± 0.169
0.794CysVal: 0.794 ± 0.206
0.233CysTrp: 0.233 ± 0.099
0.327CysTyr: 0.327 ± 0.133
0.0CysXaa: 0.0 ± 0.0
Asp
7.142AspAla: 7.142 ± 0.552
0.98AspCys: 0.98 ± 0.243
4.621AspAsp: 4.621 ± 0.62
5.181AspGlu: 5.181 ± 0.581
1.68AspPhe: 1.68 ± 0.229
5.928AspGly: 5.928 ± 0.564
1.447AspHis: 1.447 ± 0.315
3.221AspIle: 3.221 ± 0.366
1.961AspLys: 1.961 ± 0.323
5.695AspLeu: 5.695 ± 0.473
1.074AspMet: 1.074 ± 0.255
1.961AspAsn: 1.961 ± 0.315
5.135AspPro: 5.135 ± 0.487
1.447AspGln: 1.447 ± 0.266
4.014AspArg: 4.014 ± 0.541
2.801AspSer: 2.801 ± 0.359
3.734AspThr: 3.734 ± 0.422
3.781AspVal: 3.781 ± 0.332
1.074AspTrp: 1.074 ± 0.229
1.774AspTyr: 1.774 ± 0.237
0.0AspXaa: 0.0 ± 0.0
Glu
7.235GluAla: 7.235 ± 0.711
0.654GluCys: 0.654 ± 0.203
3.594GluAsp: 3.594 ± 0.547
2.241GluGlu: 2.241 ± 0.317
2.147GluPhe: 2.147 ± 0.369
4.575GluGly: 4.575 ± 0.566
1.26GluHis: 1.26 ± 0.283
3.781GluIle: 3.781 ± 0.471
1.68GluLys: 1.68 ± 0.28
6.442GluLeu: 6.442 ± 0.564
0.794GluMet: 0.794 ± 0.205
1.12GluAsn: 1.12 ± 0.226
3.268GluPro: 3.268 ± 0.489
2.521GluGln: 2.521 ± 0.324
4.388GluArg: 4.388 ± 0.436
1.867GluSer: 1.867 ± 0.24
3.688GluThr: 3.688 ± 0.396
5.975GluVal: 5.975 ± 0.62
1.12GluTrp: 1.12 ± 0.228
1.027GluTyr: 1.027 ± 0.214
0.0GluXaa: 0.0 ± 0.0
Phe
2.941PheAla: 2.941 ± 0.35
0.28PheCys: 0.28 ± 0.114
1.54PheAsp: 1.54 ± 0.252
1.26PheGlu: 1.26 ± 0.242
0.42PhePhe: 0.42 ± 0.121
2.987PheGly: 2.987 ± 0.388
0.467PheHis: 0.467 ± 0.133
0.607PheIle: 0.607 ± 0.164
0.98PheLys: 0.98 ± 0.232
1.447PheLeu: 1.447 ± 0.28
0.28PheMet: 0.28 ± 0.122
0.887PheAsn: 0.887 ± 0.156
1.307PhePro: 1.307 ± 0.235
0.56PheGln: 0.56 ± 0.151
1.587PheArg: 1.587 ± 0.241
1.167PheSer: 1.167 ± 0.224
1.587PheThr: 1.587 ± 0.247
1.4PheVal: 1.4 ± 0.23
0.327PheTrp: 0.327 ± 0.122
0.7PheTyr: 0.7 ± 0.168
0.0PheXaa: 0.0 ± 0.0
Gly
9.569GlyAla: 9.569 ± 1.344
1.26GlyCys: 1.26 ± 0.329
5.275GlyAsp: 5.275 ± 0.626
5.135GlyGlu: 5.135 ± 0.54
1.914GlyPhe: 1.914 ± 0.332
13.21GlyGly: 13.21 ± 2.521
2.054GlyHis: 2.054 ± 0.331
4.948GlyIle: 4.948 ± 0.377
3.641GlyLys: 3.641 ± 0.416
7.935GlyLeu: 7.935 ± 0.832
2.101GlyMet: 2.101 ± 0.264
2.474GlyAsn: 2.474 ± 0.416
4.715GlyPro: 4.715 ± 0.563
3.548GlyGln: 3.548 ± 0.48
6.068GlyArg: 6.068 ± 0.577
4.995GlySer: 4.995 ± 0.462
6.908GlyThr: 6.908 ± 0.702
6.628GlyVal: 6.628 ± 0.62
1.961GlyTrp: 1.961 ± 0.338
2.801GlyTyr: 2.801 ± 0.305
0.0GlyXaa: 0.0 ± 0.0
His
1.867HisAla: 1.867 ± 0.318
0.28HisCys: 0.28 ± 0.128
1.214HisAsp: 1.214 ± 0.285
1.587HisGlu: 1.587 ± 0.303
0.513HisPhe: 0.513 ± 0.127
1.774HisGly: 1.774 ± 0.284
1.027HisHis: 1.027 ± 0.328
1.54HisIle: 1.54 ± 0.281
0.42HisLys: 0.42 ± 0.155
1.354HisLeu: 1.354 ± 0.311
0.56HisMet: 0.56 ± 0.178
0.373HisAsn: 0.373 ± 0.12
1.12HisPro: 1.12 ± 0.24
0.327HisGln: 0.327 ± 0.132
2.194HisArg: 2.194 ± 0.372
0.84HisSer: 0.84 ± 0.184
2.007HisThr: 2.007 ± 0.339
1.214HisVal: 1.214 ± 0.259
0.187HisTrp: 0.187 ± 0.079
0.654HisTyr: 0.654 ± 0.214
0.0HisXaa: 0.0 ± 0.0
Ile
5.741IleAla: 5.741 ± 0.489
0.467IleCys: 0.467 ± 0.137
3.874IleAsp: 3.874 ± 0.413
4.761IleGlu: 4.761 ± 0.535
0.887IlePhe: 0.887 ± 0.166
5.135IleGly: 5.135 ± 0.53
0.654IleHis: 0.654 ± 0.186
1.634IleIle: 1.634 ± 0.322
1.307IleLys: 1.307 ± 0.273
2.847IleLeu: 2.847 ± 0.346
0.84IleMet: 0.84 ± 0.183
1.54IleAsn: 1.54 ± 0.222
2.754IlePro: 2.754 ± 0.314
0.934IleGln: 0.934 ± 0.232
2.241IleArg: 2.241 ± 0.402
2.381IleSer: 2.381 ± 0.3
3.781IleThr: 3.781 ± 0.377
3.268IleVal: 3.268 ± 0.4
0.373IleTrp: 0.373 ± 0.115
0.84IleTyr: 0.84 ± 0.188
0.0IleXaa: 0.0 ± 0.0
Lys
3.968LysAla: 3.968 ± 0.481
0.14LysCys: 0.14 ± 0.08
1.447LysAsp: 1.447 ± 0.227
0.84LysGlu: 0.84 ± 0.273
0.654LysPhe: 0.654 ± 0.19
2.567LysGly: 2.567 ± 0.268
0.56LysHis: 0.56 ± 0.185
1.68LysIle: 1.68 ± 0.229
0.887LysLys: 0.887 ± 0.223
2.987LysLeu: 2.987 ± 0.374
0.373LysMet: 0.373 ± 0.135
0.7LysAsn: 0.7 ± 0.208
1.354LysPro: 1.354 ± 0.294
1.354LysGln: 1.354 ± 0.261
2.194LysArg: 2.194 ± 0.364
1.4LysSer: 1.4 ± 0.238
2.381LysThr: 2.381 ± 0.364
2.661LysVal: 2.661 ± 0.315
0.327LysTrp: 0.327 ± 0.109
0.654LysTyr: 0.654 ± 0.154
0.0LysXaa: 0.0 ± 0.0
Leu
11.436LeuAla: 11.436 ± 0.877
1.167LeuCys: 1.167 ± 0.296
6.302LeuAsp: 6.302 ± 0.696
3.268LeuGlu: 3.268 ± 0.308
1.914LeuPhe: 1.914 ± 0.362
7.562LeuGly: 7.562 ± 1.008
1.027LeuHis: 1.027 ± 0.211
3.734LeuIle: 3.734 ± 0.427
2.287LeuLys: 2.287 ± 0.387
5.181LeuLeu: 5.181 ± 0.522
1.68LeuMet: 1.68 ± 0.275
3.361LeuAsn: 3.361 ± 0.436
5.928LeuPro: 5.928 ± 0.543
1.54LeuGln: 1.54 ± 0.255
4.061LeuArg: 4.061 ± 0.536
3.968LeuSer: 3.968 ± 0.401
7.095LeuThr: 7.095 ± 0.535
4.808LeuVal: 4.808 ± 0.546
1.4LeuTrp: 1.4 ± 0.273
1.727LeuTyr: 1.727 ± 0.3
0.0LeuXaa: 0.0 ± 0.0
Met
2.661MetAla: 2.661 ± 0.376
0.28MetCys: 0.28 ± 0.111
0.654MetAsp: 0.654 ± 0.159
0.654MetGlu: 0.654 ± 0.149
0.747MetPhe: 0.747 ± 0.164
1.494MetGly: 1.494 ± 0.246
0.467MetHis: 0.467 ± 0.173
0.794MetIle: 0.794 ± 0.182
0.654MetLys: 0.654 ± 0.173
1.494MetLeu: 1.494 ± 0.208
0.373MetMet: 0.373 ± 0.121
0.42MetAsn: 0.42 ± 0.148
1.447MetPro: 1.447 ± 0.241
0.373MetGln: 0.373 ± 0.129
1.447MetArg: 1.447 ± 0.265
1.867MetSer: 1.867 ± 0.252
2.474MetThr: 2.474 ± 0.299
1.354MetVal: 1.354 ± 0.241
0.513MetTrp: 0.513 ± 0.146
0.513MetTyr: 0.513 ± 0.157
0.0MetXaa: 0.0 ± 0.0
Asn
3.548AsnAla: 3.548 ± 0.36
0.187AsnCys: 0.187 ± 0.099
2.007AsnAsp: 2.007 ± 0.321
1.26AsnGlu: 1.26 ± 0.213
0.327AsnPhe: 0.327 ± 0.111
3.594AsnGly: 3.594 ± 0.453
0.56AsnHis: 0.56 ± 0.168
1.4AsnIle: 1.4 ± 0.273
1.027AsnLys: 1.027 ± 0.245
1.914AsnLeu: 1.914 ± 0.269
0.467AsnMet: 0.467 ± 0.118
1.26AsnAsn: 1.26 ± 0.261
1.82AsnPro: 1.82 ± 0.31
0.42AsnGln: 0.42 ± 0.138
1.961AsnArg: 1.961 ± 0.331
1.634AsnSer: 1.634 ± 0.3
2.661AsnThr: 2.661 ± 0.415
1.82AsnVal: 1.82 ± 0.251
0.373AsnTrp: 0.373 ± 0.151
0.934AsnTyr: 0.934 ± 0.216
0.0AsnXaa: 0.0 ± 0.0
Pro
7.375ProAla: 7.375 ± 0.562
0.373ProCys: 0.373 ± 0.127
4.154ProAsp: 4.154 ± 0.508
5.555ProGlu: 5.555 ± 0.736
1.26ProPhe: 1.26 ± 0.231
6.115ProGly: 6.115 ± 0.751
1.447ProHis: 1.447 ± 0.302
2.567ProIle: 2.567 ± 0.412
1.26ProLys: 1.26 ± 0.239
3.548ProLeu: 3.548 ± 0.334
1.12ProMet: 1.12 ± 0.195
1.961ProAsn: 1.961 ± 0.278
4.481ProPro: 4.481 ± 0.579
1.774ProGln: 1.774 ± 0.289
3.734ProArg: 3.734 ± 0.467
2.381ProSer: 2.381 ± 0.375
3.874ProThr: 3.874 ± 0.312
4.948ProVal: 4.948 ± 0.539
1.4ProTrp: 1.4 ± 0.199
1.027ProTyr: 1.027 ± 0.247
0.0ProXaa: 0.0 ± 0.0
Gln
4.201GlnAla: 4.201 ± 0.614
0.28GlnCys: 0.28 ± 0.125
1.12GlnAsp: 1.12 ± 0.236
0.84GlnGlu: 0.84 ± 0.184
0.98GlnPhe: 0.98 ± 0.202
2.241GlnGly: 2.241 ± 0.35
0.98GlnHis: 0.98 ± 0.227
2.054GlnIle: 2.054 ± 0.282
0.794GlnLys: 0.794 ± 0.24
2.474GlnLeu: 2.474 ± 0.339
0.607GlnMet: 0.607 ± 0.152
0.7GlnAsn: 0.7 ± 0.191
1.54GlnPro: 1.54 ± 0.241
1.54GlnGln: 1.54 ± 0.218
2.987GlnArg: 2.987 ± 0.454
1.727GlnSer: 1.727 ± 0.252
2.194GlnThr: 2.194 ± 0.297
2.007GlnVal: 2.007 ± 0.273
0.747GlnTrp: 0.747 ± 0.186
0.654GlnTyr: 0.654 ± 0.162
0.0GlnXaa: 0.0 ± 0.0
Arg
7.142ArgAla: 7.142 ± 0.623
0.7ArgCys: 0.7 ± 0.209
4.108ArgAsp: 4.108 ± 0.409
5.041ArgGlu: 5.041 ± 0.626
1.54ArgPhe: 1.54 ± 0.265
4.154ArgGly: 4.154 ± 0.442
1.82ArgHis: 1.82 ± 0.343
2.381ArgIle: 2.381 ± 0.352
1.867ArgLys: 1.867 ± 0.303
5.928ArgLeu: 5.928 ± 0.462
1.774ArgMet: 1.774 ± 0.371
2.521ArgAsn: 2.521 ± 0.356
4.014ArgPro: 4.014 ± 0.511
2.474ArgGln: 2.474 ± 0.338
6.348ArgArg: 6.348 ± 0.811
2.847ArgSer: 2.847 ± 0.292
4.108ArgThr: 4.108 ± 0.394
5.368ArgVal: 5.368 ± 0.644
1.68ArgTrp: 1.68 ± 0.275
2.287ArgTyr: 2.287 ± 0.431
0.0ArgXaa: 0.0 ± 0.0
Ser
5.088SerAla: 5.088 ± 0.54
0.607SerCys: 0.607 ± 0.184
3.268SerAsp: 3.268 ± 0.278
2.241SerGlu: 2.241 ± 0.319
0.794SerPhe: 0.794 ± 0.236
5.788SerGly: 5.788 ± 0.645
0.7SerHis: 0.7 ± 0.191
1.727SerIle: 1.727 ± 0.305
1.214SerLys: 1.214 ± 0.198
3.968SerLeu: 3.968 ± 0.582
1.354SerMet: 1.354 ± 0.261
1.12SerAsn: 1.12 ± 0.248
3.081SerPro: 3.081 ± 0.376
1.961SerGln: 1.961 ± 0.277
3.594SerArg: 3.594 ± 0.305
2.521SerSer: 2.521 ± 0.38
3.874SerThr: 3.874 ± 0.41
3.781SerVal: 3.781 ± 0.428
1.027SerTrp: 1.027 ± 0.17
1.4SerTyr: 1.4 ± 0.2
0.0SerXaa: 0.0 ± 0.0
Thr
8.636ThrAla: 8.636 ± 0.536
0.42ThrCys: 0.42 ± 0.13
4.995ThrAsp: 4.995 ± 0.477
3.594ThrGlu: 3.594 ± 0.408
1.587ThrPhe: 1.587 ± 0.264
7.048ThrGly: 7.048 ± 0.488
1.167ThrHis: 1.167 ± 0.202
3.641ThrIle: 3.641 ± 0.42
2.521ThrLys: 2.521 ± 0.315
5.135ThrLeu: 5.135 ± 0.443
1.634ThrMet: 1.634 ± 0.287
2.054ThrAsn: 2.054 ± 0.391
5.041ThrPro: 5.041 ± 0.576
2.054ThrGln: 2.054 ± 0.293
3.874ThrArg: 3.874 ± 0.456
4.294ThrSer: 4.294 ± 0.377
3.921ThrThr: 3.921 ± 0.44
6.535ThrVal: 6.535 ± 0.564
1.214ThrTrp: 1.214 ± 0.273
1.634ThrTyr: 1.634 ± 0.286
0.0ThrXaa: 0.0 ± 0.0
Val
8.636ValAla: 8.636 ± 0.551
1.074ValCys: 1.074 ± 0.249
4.434ValAsp: 4.434 ± 0.444
5.741ValGlu: 5.741 ± 0.667
1.447ValPhe: 1.447 ± 0.228
7.702ValGly: 7.702 ± 0.509
1.68ValHis: 1.68 ± 0.245
3.548ValIle: 3.548 ± 0.332
1.914ValLys: 1.914 ± 0.289
6.488ValLeu: 6.488 ± 0.483
1.447ValMet: 1.447 ± 0.268
2.194ValAsn: 2.194 ± 0.285
3.594ValPro: 3.594 ± 0.335
1.961ValGln: 1.961 ± 0.261
4.434ValArg: 4.434 ± 0.478
3.968ValSer: 3.968 ± 0.409
5.321ValThr: 5.321 ± 0.57
6.115ValVal: 6.115 ± 0.678
1.82ValTrp: 1.82 ± 0.293
1.914ValTyr: 1.914 ± 0.372
0.0ValXaa: 0.0 ± 0.0
Trp
2.381TrpAla: 2.381 ± 0.409
0.513TrpCys: 0.513 ± 0.17
1.307TrpAsp: 1.307 ± 0.277
0.467TrpGlu: 0.467 ± 0.142
0.654TrpPhe: 0.654 ± 0.256
1.027TrpGly: 1.027 ± 0.203
0.513TrpHis: 0.513 ± 0.16
0.98TrpIle: 0.98 ± 0.2
0.233TrpLys: 0.233 ± 0.101
1.447TrpLeu: 1.447 ± 0.251
0.56TrpMet: 0.56 ± 0.146
0.327TrpAsn: 0.327 ± 0.103
0.84TrpPro: 0.84 ± 0.221
0.607TrpGln: 0.607 ± 0.184
1.354TrpArg: 1.354 ± 0.222
1.307TrpSer: 1.307 ± 0.219
1.447TrpThr: 1.447 ± 0.276
1.587TrpVal: 1.587 ± 0.297
0.42TrpTrp: 0.42 ± 0.133
0.42TrpTyr: 0.42 ± 0.141
0.0TrpXaa: 0.0 ± 0.0
Tyr
3.314TyrAla: 3.314 ± 0.366
0.28TyrCys: 0.28 ± 0.121
2.054TyrAsp: 2.054 ± 0.269
1.587TyrGlu: 1.587 ± 0.315
0.607TyrPhe: 0.607 ± 0.166
2.101TyrGly: 2.101 ± 0.324
0.467TyrHis: 0.467 ± 0.134
1.074TyrIle: 1.074 ± 0.194
0.56TyrLys: 0.56 ± 0.171
1.961TyrLeu: 1.961 ± 0.328
0.28TyrMet: 0.28 ± 0.108
0.747TyrAsn: 0.747 ± 0.166
1.074TyrPro: 1.074 ± 0.233
0.84TyrGln: 0.84 ± 0.188
2.101TyrArg: 2.101 ± 0.334
1.027TyrSer: 1.027 ± 0.192
1.634TyrThr: 1.634 ± 0.248
2.334TyrVal: 2.334 ± 0.259
0.233TyrTrp: 0.233 ± 0.089
0.56TyrTyr: 0.56 ± 0.15
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 99 proteins (21424 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski