Pectobacterium phage vB_PatP_CB5

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Autographiviridae; Corkvirinae; Phimunavirus; Pectobacterium virus CB5

Average proteome isoelectric point is 6.65

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 59 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2U7NHL0|A0A2U7NHL0_9CAUD Endonuclease OS=Pectobacterium phage vB_PatP_CB5 OX=1983582 GN=CB5_25 PE=4 SV=1
MM1 pKa = 7.5RR2 pKa = 11.84RR3 pKa = 11.84DD4 pKa = 3.65GVDD7 pKa = 3.65LDD9 pKa = 4.31SCMSRR14 pKa = 11.84PWGDD18 pKa = 3.43YY19 pKa = 8.58NCPHH23 pKa = 6.75GVHH26 pKa = 6.74PCDD29 pKa = 4.33AYY31 pKa = 11.5SSAHH35 pKa = 6.05YY36 pKa = 10.15GAGDD40 pKa = 3.42NGLVLVEE47 pKa = 4.34AQHH50 pKa = 6.3AGKK53 pKa = 9.31PVGRR57 pKa = 11.84GILNTRR63 pKa = 11.84NNQIVRR69 pKa = 11.84WYY71 pKa = 9.82GEE73 pKa = 3.74YY74 pKa = 9.69RR75 pKa = 11.84AKK77 pKa = 10.85VQLCNAYY84 pKa = 9.97GIKK87 pKa = 9.98EE88 pKa = 4.18DD89 pKa = 4.07SDD91 pKa = 3.9ALEE94 pKa = 4.72DD95 pKa = 3.37SWLAMIGTPDD105 pKa = 3.74VFAGPYY111 pKa = 9.51VDD113 pKa = 5.28GDD115 pKa = 3.54IAAGAVQEE123 pKa = 4.15STYY126 pKa = 10.84RR127 pKa = 11.84VVLGCDD133 pKa = 3.26GVGLEE138 pKa = 4.39EE139 pKa = 4.15TGGYY143 pKa = 9.94YY144 pKa = 10.56YY145 pKa = 9.96STEE148 pKa = 3.8DD149 pKa = 4.03RR150 pKa = 11.84QRR152 pKa = 11.84CCIADD157 pKa = 3.5EE158 pKa = 5.3DD159 pKa = 4.33YY160 pKa = 11.37PEE162 pKa = 6.85DD163 pKa = 3.79DD164 pKa = 4.1MRR166 pKa = 11.84YY167 pKa = 9.04QAYY170 pKa = 10.53NDD172 pKa = 3.25TWYY175 pKa = 10.87HH176 pKa = 6.52PDD178 pKa = 3.25NTGDD182 pKa = 3.67YY183 pKa = 8.86CACEE187 pKa = 4.14CPVTGEE193 pKa = 4.16YY194 pKa = 10.43FHH196 pKa = 8.74VDD198 pKa = 2.32IGHH201 pKa = 7.41RR202 pKa = 11.84LTIDD206 pKa = 3.43GEE208 pKa = 4.46EE209 pKa = 4.35VLVSWAGYY217 pKa = 8.98NHH219 pKa = 7.18TDD221 pKa = 2.83TRR223 pKa = 11.84GYY225 pKa = 9.39TNLGGNIGYY234 pKa = 7.93TRR236 pKa = 11.84DD237 pKa = 3.41TEE239 pKa = 4.46QYY241 pKa = 10.5RR242 pKa = 11.84LLANGDD248 pKa = 3.38WVHH251 pKa = 7.34ADD253 pKa = 3.09DD254 pKa = 5.47AVYY257 pKa = 10.71DD258 pKa = 4.03KK259 pKa = 10.79EE260 pKa = 4.26TDD262 pKa = 3.0DD263 pKa = 4.27WYY265 pKa = 10.22TEE267 pKa = 3.93SEE269 pKa = 4.38YY270 pKa = 11.52ADD272 pKa = 4.75LISEE276 pKa = 4.33RR277 pKa = 11.84EE278 pKa = 3.96EE279 pKa = 4.37GAQDD283 pKa = 3.28AAA285 pKa = 4.43

Molecular weight: 31.87 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2U7MVS8|A0A2U7MVS8_9CAUD Putative structural protein OS=Pectobacterium phage vB_PatP_CB5 OX=1983582 GN=CB5_43 PE=4 SV=1
MM1 pKa = 7.63SDD3 pKa = 2.58WYY5 pKa = 10.54YY6 pKa = 11.2DD7 pKa = 3.51KK8 pKa = 11.26EE9 pKa = 4.56SGVVYY14 pKa = 10.67RR15 pKa = 11.84NGRR18 pKa = 11.84PMTLLGKK25 pKa = 10.04DD26 pKa = 3.42GYY28 pKa = 11.79VRR30 pKa = 11.84MRR32 pKa = 11.84YY33 pKa = 9.19KK34 pKa = 10.81GRR36 pKa = 11.84VEE38 pKa = 3.8RR39 pKa = 11.84AHH41 pKa = 6.28RR42 pKa = 11.84LAYY45 pKa = 9.47ILQGLPLPKK54 pKa = 10.04QVDD57 pKa = 4.17HH58 pKa = 7.25INGNRR63 pKa = 11.84AANRR67 pKa = 11.84WVNLRR72 pKa = 11.84PATNTQNQYY81 pKa = 9.2NRR83 pKa = 11.84RR84 pKa = 11.84PASKK88 pKa = 10.26LGHH91 pKa = 6.28KK92 pKa = 9.88KK93 pKa = 9.55GAYY96 pKa = 8.25MNIGGRR102 pKa = 11.84TWYY105 pKa = 10.78SLLRR109 pKa = 11.84YY110 pKa = 8.55NGKK113 pKa = 10.12RR114 pKa = 11.84IYY116 pKa = 10.75LGTFKK121 pKa = 10.75TEE123 pKa = 4.3DD124 pKa = 3.78EE125 pKa = 3.93AHH127 pKa = 6.27AAYY130 pKa = 9.37TEE132 pKa = 4.11ASKK135 pKa = 10.61KK136 pKa = 7.97YY137 pKa = 9.36HH138 pKa = 6.29GNFSRR143 pKa = 11.84TEE145 pKa = 3.65

Molecular weight: 16.92 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

59

0

59

14074

42

1263

238.5

26.36

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.692 ± 0.399

1.208 ± 0.167

6.125 ± 0.217

5.194 ± 0.317

3.119 ± 0.164

7.695 ± 0.287

1.968 ± 0.176

4.526 ± 0.191

4.704 ± 0.333

8.633 ± 0.264

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.693 ± 0.158

4.405 ± 0.247

3.929 ± 0.203

4.81 ± 0.396

5.656 ± 0.316

6.48 ± 0.289

6.48 ± 0.36

7.269 ± 0.257

1.386 ± 0.133

4.029 ± 0.269
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski