Amino acid dipepetide frequency for Echeneis naucrates (Live sharksucker)

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	5.818AlaAla: 5.818 ± 0.023	1.302AlaCys: 1.302 ± 0.007	3.158AlaAsp: 3.158 ± 0.011	4.481AlaGlu: 4.481 ± 0.017	2.452AlaPhe: 2.452 ± 0.009	4.143AlaGly: 4.143 ± 0.016	1.489AlaHis: 1.489 ± 0.007	2.934AlaIle: 2.934 ± 0.01	3.441AlaLys: 3.441 ± 0.014	6.545AlaLeu: 6.545 ± 0.017	1.63AlaMet: 1.63 ± 0.007	2.253AlaAsn: 2.253 ± 0.008	3.144AlaPro: 3.144 ± 0.012	2.864AlaGln: 2.864 ± 0.013	3.054AlaArg: 3.054 ± 0.009	5.058AlaSer: 5.058 ± 0.014	3.377AlaThr: 3.377 ± 0.012	4.825AlaVal: 4.825 ± 0.012	0.659AlaTrp: 0.659 ± 0.005	1.602AlaTyr: 1.602 ± 0.007	0.0AlaXaa: 0.0 ± 0.0
Cys	1.188CysAla: 1.188 ± 0.008	0.663CysCys: 0.663 ± 0.006	1.168CysAsp: 1.168 ± 0.009	1.272CysGlu: 1.272 ± 0.01	0.982CysPhe: 0.982 ± 0.006	1.52CysGly: 1.52 ± 0.012	0.692CysHis: 0.692 ± 0.006	1.088CysIle: 1.088 ± 0.007	1.19CysLys: 1.19 ± 0.007	2.305CysLeu: 2.305 ± 0.011	0.493CysMet: 0.493 ± 0.004	0.864CysAsn: 0.864 ± 0.006	1.294CysPro: 1.294 ± 0.01	1.1CysGln: 1.1 ± 0.009	1.322CysArg: 1.322 ± 0.008	2.141CysSer: 2.141 ± 0.013	1.205CysThr: 1.205 ± 0.008	1.658CysVal: 1.658 ± 0.01	0.314CysTrp: 0.314 ± 0.003	0.65CysTyr: 0.65 ± 0.005	0.0CysXaa: 0.0 ± 0.0
Asp	2.916AspAla: 2.916 ± 0.01	1.19AspCys: 1.19 ± 0.008	3.105AspAsp: 3.105 ± 0.013	3.679AspGlu: 3.679 ± 0.012	2.244AspPhe: 2.244 ± 0.01	3.622AspGly: 3.622 ± 0.016	1.229AspHis: 1.229 ± 0.006	2.941AspIle: 2.941 ± 0.011	2.838AspLys: 2.838 ± 0.011	5.206AspLeu: 5.206 ± 0.016	1.336AspMet: 1.336 ± 0.007	2.064AspAsn: 2.064 ± 0.009	2.846AspPro: 2.846 ± 0.01	2.004AspGln: 2.004 ± 0.007	2.731AspArg: 2.731 ± 0.012	4.313AspSer: 4.313 ± 0.012	2.655AspThr: 2.655 ± 0.009	3.312AspVal: 3.312 ± 0.011	0.708AspTrp: 0.708 ± 0.005	1.643AspTyr: 1.643 ± 0.008	0.0AspXaa: 0.0 ± 0.0
Glu	4.652GluAla: 4.652 ± 0.016	1.312GluCys: 1.312 ± 0.011	4.27GluAsp: 4.27 ± 0.012	7.221GluGlu: 7.221 ± 0.031	2.062GluPhe: 2.062 ± 0.008	4.102GluGly: 4.102 ± 0.013	1.478GluHis: 1.478 ± 0.006	3.03GluIle: 3.03 ± 0.011	4.611GluLys: 4.611 ± 0.019	6.407GluLeu: 6.407 ± 0.021	1.794GluMet: 1.794 ± 0.008	2.755GluAsn: 2.755 ± 0.01	2.604GluPro: 2.604 ± 0.011	3.048GluGln: 3.048 ± 0.013	4.132GluArg: 4.132 ± 0.017	4.133GluSer: 4.133 ± 0.016	3.381GluThr: 3.381 ± 0.011	4.382GluVal: 4.382 ± 0.011	0.696GluTrp: 0.696 ± 0.005	1.72GluTyr: 1.72 ± 0.008	0.0GluXaa: 0.0 ± 0.0
Phe	2.023PheAla: 2.023 ± 0.008	1.014PheCys: 1.014 ± 0.006	1.923PheAsp: 1.923 ± 0.008	2.004PheGlu: 2.004 ± 0.009	1.825PhePhe: 1.825 ± 0.009	2.31PheGly: 2.31 ± 0.01	1.108PheHis: 1.108 ± 0.006	2.124PheIle: 2.124 ± 0.01	1.933PheLys: 1.933 ± 0.008	4.151PheLeu: 4.151 ± 0.016	0.869PheMet: 0.869 ± 0.006	1.58PheAsn: 1.58 ± 0.007	1.87PhePro: 1.87 ± 0.008	1.698PheGln: 1.698 ± 0.007	1.92PheArg: 1.92 ± 0.008	3.453PheSer: 3.453 ± 0.012	2.409PheThr: 2.409 ± 0.009	2.372PheVal: 2.372 ± 0.009	0.486PheTrp: 0.486 ± 0.004	1.313PheTyr: 1.313 ± 0.007	0.0PheXaa: 0.0 ± 0.0
Gly	3.869GlyAla: 3.869 ± 0.015	1.243GlyCys: 1.243 ± 0.007	3.181GlyAsp: 3.181 ± 0.011	3.987GlyGlu: 3.987 ± 0.014	2.497GlyPhe: 2.497 ± 0.011	5.025GlyGly: 5.025 ± 0.021	1.679GlyHis: 1.679 ± 0.008	2.765GlyIle: 2.765 ± 0.009	3.677GlyLys: 3.677 ± 0.012	5.505GlyLeu: 5.505 ± 0.017	1.522GlyMet: 1.522 ± 0.007	2.479GlyAsn: 2.479 ± 0.01	3.139GlyPro: 3.139 ± 0.024	2.755GlyGln: 2.755 ± 0.011	3.504GlyArg: 3.504 ± 0.012	5.312GlySer: 5.312 ± 0.016	3.411GlyThr: 3.411 ± 0.011	3.941GlyVal: 3.941 ± 0.012	0.782GlyTrp: 0.782 ± 0.006	1.92GlyTyr: 1.92 ± 0.009	0.0GlyXaa: 0.0 ± 0.0
His	1.322HisAla: 1.322 ± 0.007	0.85HisCys: 0.85 ± 0.007	1.027HisAsp: 1.027 ± 0.005	1.265HisGlu: 1.265 ± 0.006	1.135HisPhe: 1.135 ± 0.006	1.564HisGly: 1.564 ± 0.008	1.025HisHis: 1.025 ± 0.008	1.448HisIle: 1.448 ± 0.006	1.315HisLys: 1.315 ± 0.006	2.802HisLeu: 2.802 ± 0.009	0.675HisMet: 0.675 ± 0.005	1.101HisAsn: 1.101 ± 0.006	1.56HisPro: 1.56 ± 0.008	1.251HisGln: 1.251 ± 0.007	1.602HisArg: 1.602 ± 0.008	2.362HisSer: 2.362 ± 0.01	1.634HisThr: 1.634 ± 0.009	1.475HisVal: 1.475 ± 0.007	0.354HisTrp: 0.354 ± 0.003	0.906HisTyr: 0.906 ± 0.005	0.0HisXaa: 0.0 ± 0.0
Ile	2.687IleAla: 2.687 ± 0.01	1.149IleCys: 1.149 ± 0.006	2.316IleAsp: 2.316 ± 0.01	2.577IleGlu: 2.577 ± 0.009	2.033IlePhe: 2.033 ± 0.008	2.436IleGly: 2.436 ± 0.01	1.391IleHis: 1.391 ± 0.006	2.678IleIle: 2.678 ± 0.012	2.769IleLys: 2.769 ± 0.011	4.602IleLeu: 4.602 ± 0.013	1.188IleMet: 1.188 ± 0.006	2.158IleAsn: 2.158 ± 0.009	2.52IlePro: 2.52 ± 0.008	2.336IleGln: 2.336 ± 0.009	2.606IleArg: 2.606 ± 0.009	3.932IleSer: 3.932 ± 0.011	2.94IleThr: 2.94 ± 0.011	2.731IleVal: 2.731 ± 0.011	0.511IleTrp: 0.511 ± 0.004	1.556IleTyr: 1.556 ± 0.007	0.0IleXaa: 0.0 ± 0.0
Lys	3.771LysAla: 3.771 ± 0.013	1.077LysCys: 1.077 ± 0.007	3.364LysAsp: 3.364 ± 0.011	4.694LysGlu: 4.694 ± 0.016	1.772LysPhe: 1.772 ± 0.008	3.236LysGly: 3.236 ± 0.014	1.45LysHis: 1.45 ± 0.007	2.686LysIle: 2.686 ± 0.011	4.462LysLys: 4.462 ± 0.017	5.251LysLeu: 5.251 ± 0.015	1.523LysMet: 1.523 ± 0.008	2.384LysAsn: 2.384 ± 0.01	2.726LysPro: 2.726 ± 0.011	2.582LysGln: 2.582 ± 0.012	3.332LysArg: 3.332 ± 0.012	3.757LysSer: 3.757 ± 0.014	3.179LysThr: 3.179 ± 0.01	3.727LysVal: 3.727 ± 0.012	0.605LysTrp: 0.605 ± 0.004	1.614LysTyr: 1.614 ± 0.008	0.0LysXaa: 0.0 ± 0.0
Leu	6.165LeuAla: 6.165 ± 0.016	2.36LeuCys: 2.36 ± 0.011	5.073LeuAsp: 5.073 ± 0.013	6.594LeuGlu: 6.594 ± 0.024	3.709LeuPhe: 3.709 ± 0.014	5.182LeuGly: 5.182 ± 0.014	2.794LeuHis: 2.794 ± 0.011	4.136LeuIle: 4.136 ± 0.013	5.884LeuLys: 5.884 ± 0.019	10.355LeuLeu: 10.355 ± 0.024	2.251LeuMet: 2.251 ± 0.008	3.812LeuAsn: 3.812 ± 0.011	5.132LeuPro: 5.132 ± 0.015	5.518LeuGln: 5.518 ± 0.019	5.761LeuArg: 5.761 ± 0.016	8.305LeuSer: 8.305 ± 0.024	5.32LeuThr: 5.32 ± 0.012	5.672LeuVal: 5.672 ± 0.016	1.113LeuTrp: 1.113 ± 0.006	2.807LeuTyr: 2.807 ± 0.01	0.0LeuXaa: 0.0 ± 0.0
Met	1.94MetAla: 1.94 ± 0.007	0.531MetCys: 0.531 ± 0.005	1.469MetAsp: 1.469 ± 0.006	2.021MetGlu: 2.021 ± 0.009	0.926MetPhe: 0.926 ± 0.006	1.438MetGly: 1.438 ± 0.007	0.507MetHis: 0.507 ± 0.004	0.967MetIle: 0.967 ± 0.006	1.558MetLys: 1.558 ± 0.007	2.203MetLeu: 2.203 ± 0.008	0.71MetMet: 0.71 ± 0.005	0.977MetAsn: 0.977 ± 0.005	1.084MetPro: 1.084 ± 0.007	1.021MetGln: 1.021 ± 0.006	1.211MetArg: 1.211 ± 0.006	1.834MetSer: 1.834 ± 0.008	1.31MetThr: 1.31 ± 0.007	1.647MetVal: 1.647 ± 0.007	0.284MetTrp: 0.284 ± 0.003	0.682MetTyr: 0.682 ± 0.004	0.0MetXaa: 0.0 ± 0.0
Asn	2.193AsnAla: 2.193 ± 0.009	0.952AsnCys: 0.952 ± 0.008	1.808AsnAsp: 1.808 ± 0.009	2.168AsnGlu: 2.168 ± 0.009	1.546AsnPhe: 1.546 ± 0.008	2.772AsnGly: 2.772 ± 0.014	1.035AsnHis: 1.035 ± 0.006	2.335AsnIle: 2.335 ± 0.009	2.307AsnLys: 2.307 ± 0.009	3.834AsnLeu: 3.834 ± 0.012	1.086AsnMet: 1.086 ± 0.005	1.871AsnAsn: 1.871 ± 0.01	2.31AsnPro: 2.31 ± 0.009	1.782AsnGln: 1.782 ± 0.008	2.019AsnArg: 2.019 ± 0.009	3.172AsnSer: 3.172 ± 0.01	2.33AsnThr: 2.33 ± 0.009	2.355AsnVal: 2.355 ± 0.01	0.472AsnTrp: 0.472 ± 0.004	1.229AsnTyr: 1.229 ± 0.007	0.0AsnXaa: 0.0 ± 0.0
Pro	3.703ProAla: 3.703 ± 0.012	1.044ProCys: 1.044 ± 0.009	2.854ProAsp: 2.854 ± 0.01	3.637ProGlu: 3.637 ± 0.013	1.824ProPhe: 1.824 ± 0.008	3.831ProGly: 3.831 ± 0.025	1.497ProHis: 1.497 ± 0.007	1.972ProIle: 1.972 ± 0.008	2.457ProLys: 2.457 ± 0.013	4.713ProLeu: 4.713 ± 0.014	1.025ProMet: 1.025 ± 0.005	1.947ProAsn: 1.947 ± 0.008	4.867ProPro: 4.867 ± 0.028	2.501ProGln: 2.501 ± 0.012	2.513ProArg: 2.513 ± 0.01	5.016ProSer: 5.016 ± 0.018	2.938ProThr: 2.938 ± 0.016	3.543ProVal: 3.543 ± 0.012	0.544ProTrp: 0.544 ± 0.004	1.413ProTyr: 1.413 ± 0.008	0.0ProXaa: 0.0 ± 0.0
Gln	3.193GlnAla: 3.193 ± 0.012	1.043GlnCys: 1.043 ± 0.009	2.355GlnAsp: 2.355 ± 0.009	3.434GlnGlu: 3.434 ± 0.015	1.472GlnPhe: 1.472 ± 0.007	2.722GlnGly: 2.722 ± 0.012	1.357GlnHis: 1.357 ± 0.008	2.063GlnIle: 2.063 ± 0.008	2.596GlnLys: 2.596 ± 0.01	4.621GlnLeu: 4.621 ± 0.015	1.167GlnMet: 1.167 ± 0.006	1.745GlnAsn: 1.745 ± 0.008	2.391GlnPro: 2.391 ± 0.012	3.208GlnGln: 3.208 ± 0.028	2.998GlnArg: 2.998 ± 0.01	3.301GlnSer: 3.301 ± 0.012	2.525GlnThr: 2.525 ± 0.009	2.911GlnVal: 2.911 ± 0.01	0.566GlnTrp: 0.566 ± 0.004	1.328GlnTyr: 1.328 ± 0.006	0.0GlnXaa: 0.0 ± 0.0
Arg	3.34ArgAla: 3.34 ± 0.011	1.246ArgCys: 1.246 ± 0.009	2.939ArgAsp: 2.939 ± 0.011	3.83ArgGlu: 3.83 ± 0.015	2.012ArgPhe: 2.012 ± 0.007	3.329ArgGly: 3.329 ± 0.012	1.526ArgHis: 1.526 ± 0.007	2.529ArgIle: 2.529 ± 0.009	3.501ArgLys: 3.501 ± 0.013	5.445ArgLeu: 5.445 ± 0.014	1.323ArgMet: 1.323 ± 0.006	2.181ArgAsn: 2.181 ± 0.009	2.745ArgPro: 2.745 ± 0.009	2.663ArgGln: 2.663 ± 0.01	3.985ArgArg: 3.985 ± 0.014	4.139ArgSer: 4.139 ± 0.014	2.837ArgThr: 2.837 ± 0.009	3.282ArgVal: 3.282 ± 0.01	0.681ArgTrp: 0.681 ± 0.005	1.588ArgTyr: 1.588 ± 0.006	0.0ArgXaa: 0.0 ± 0.0
Ser	5.191SerAla: 5.191 ± 0.015	1.966SerCys: 1.966 ± 0.012	4.201SerAsp: 4.201 ± 0.013	4.668SerGlu: 4.668 ± 0.013	3.144SerPhe: 3.144 ± 0.01	5.33SerGly: 5.33 ± 0.016	2.229SerHis: 2.229 ± 0.01	3.468SerIle: 3.468 ± 0.011	3.924SerLys: 3.924 ± 0.012	8.159SerLeu: 8.159 ± 0.019	1.814SerMet: 1.814 ± 0.008	3.026SerAsn: 3.026 ± 0.01	5.345SerPro: 5.345 ± 0.02	3.676SerGln: 3.676 ± 0.012	4.205SerArg: 4.205 ± 0.014	9.112SerSer: 9.112 ± 0.037	4.581SerThr: 4.581 ± 0.021	5.249SerVal: 5.249 ± 0.014	0.978SerTrp: 0.978 ± 0.006	2.167SerTyr: 2.167 ± 0.008	0.0SerXaa: 0.0 ± 0.0
Thr	3.921ThrAla: 3.921 ± 0.011	1.368ThrCys: 1.368 ± 0.01	2.931ThrAsp: 2.931 ± 0.01	3.713ThrGlu: 3.713 ± 0.013	2.205ThrPhe: 2.205 ± 0.009	3.701ThrGly: 3.701 ± 0.013	1.424ThrHis: 1.424 ± 0.007	2.564ThrIle: 2.564 ± 0.01	2.73ThrLys: 2.73 ± 0.01	5.486ThrLeu: 5.486 ± 0.012	1.255ThrMet: 1.255 ± 0.006	2.006ThrAsn: 2.006 ± 0.008	3.395ThrPro: 3.395 ± 0.015	2.367ThrGln: 2.367 ± 0.01	2.432ThrArg: 2.432 ± 0.01	4.551ThrSer: 4.551 ± 0.015	3.305ThrThr: 3.305 ± 0.04	4.164ThrVal: 4.164 ± 0.013	0.663ThrTrp: 0.663 ± 0.005	1.504ThrTyr: 1.504 ± 0.007	0.0ThrXaa: 0.0 ± 0.0
Val	4.154ValAla: 4.154 ± 0.011	1.786ValCys: 1.786 ± 0.01	3.311ValAsp: 3.311 ± 0.013	4.064ValGlu: 4.064 ± 0.013	2.773ValPhe: 2.773 ± 0.01	3.546ValGly: 3.546 ± 0.013	1.633ValHis: 1.633 ± 0.007	3.159ValIle: 3.159 ± 0.01	3.673ValLys: 3.673 ± 0.012	6.307ValLeu: 6.307 ± 0.016	1.579ValMet: 1.579 ± 0.007	2.56ValAsn: 2.56 ± 0.008	3.118ValPro: 3.118 ± 0.011	2.793ValGln: 2.793 ± 0.009	3.33ValArg: 3.33 ± 0.01	5.244ValSer: 5.244 ± 0.014	3.898ValThr: 3.898 ± 0.014	4.451ValVal: 4.451 ± 0.015	0.795ValTrp: 0.795 ± 0.005	1.886ValTyr: 1.886 ± 0.008	0.0ValXaa: 0.0 ± 0.0
Trp	0.695TrpAla: 0.695 ± 0.005	0.259TrpCys: 0.259 ± 0.003	0.662TrpAsp: 0.662 ± 0.005	0.77TrpGlu: 0.77 ± 0.005	0.468TrpPhe: 0.468 ± 0.004	0.633TrpGly: 0.633 ± 0.005	0.282TrpHis: 0.282 ± 0.003	0.59TrpIle: 0.59 ± 0.005	0.739TrpLys: 0.739 ± 0.005	1.203TrpLeu: 1.203 ± 0.007	0.361TrpMet: 0.361 ± 0.003	0.532TrpAsn: 0.532 ± 0.004	0.436TrpPro: 0.436 ± 0.004	0.514TrpGln: 0.514 ± 0.004	0.728TrpArg: 0.728 ± 0.005	0.957TrpSer: 0.957 ± 0.006	0.723TrpThr: 0.723 ± 0.006	0.707TrpVal: 0.707 ± 0.005	0.211TrpTrp: 0.211 ± 0.002	0.353TrpTyr: 0.353 ± 0.004	0.0TrpXaa: 0.0 ± 0.0
Tyr	1.49TyrAla: 1.49 ± 0.007	0.754TyrCys: 0.754 ± 0.005	1.47TyrAsp: 1.47 ± 0.008	1.711TyrGlu: 1.711 ± 0.008	1.303TyrPhe: 1.303 ± 0.007	1.749TyrGly: 1.749 ± 0.007	0.849TyrHis: 0.849 ± 0.005	1.637TyrIle: 1.637 ± 0.007	1.587TyrLys: 1.587 ± 0.007	2.841TyrLeu: 2.841 ± 0.01	0.718TyrMet: 0.718 ± 0.005	1.281TyrAsn: 1.281 ± 0.006	1.311TyrPro: 1.311 ± 0.006	1.315TyrGln: 1.315 ± 0.007	1.735TyrArg: 1.735 ± 0.008	2.342TyrSer: 2.342 ± 0.009	1.727TyrThr: 1.727 ± 0.009	1.659TyrVal: 1.659 ± 0.008	0.401TyrTrp: 0.401 ± 0.005	1.046TyrTyr: 1.046 ± 0.006	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.002XaaXaa: 0.002 ± 0.001

Statistics based on 54685 proteins (36544125 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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