Klebsiella pneumoniae subsp. pneumoniae (strain ATCC 700721 / MGH 78578)

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Klebsiella; Klebsiella pneumoniae; Klebsiella pneumoniae subsp. pneumoniae

Average proteome isoelectric point is 6.56

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5126 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A6TE21|A6TE21_KLEP7 3' 5'-cyclic adenosine monophosphate phosphodiesterase CpdA OS=Klebsiella pneumoniae subsp. pneumoniae (strain ATCC 700721 / MGH 78578) OX=272620 GN=cpdA PE=3 SV=1
MM1 pKa = 7.59SIKK4 pKa = 10.42KK5 pKa = 10.06SVIAAAVFASLSGVSMMANAAPSADD30 pKa = 3.41VTLEE34 pKa = 4.18GIITNSTCDD43 pKa = 3.29VSVNNGLATLNVGVYY58 pKa = 9.11KK59 pKa = 10.86ASSFTPNTQQGSVALPVEE77 pKa = 4.45LTNCADD83 pKa = 3.4EE84 pKa = 4.41TGALIIQGQSASSDD98 pKa = 3.28PSKK101 pKa = 11.06QLFTAATGDD110 pKa = 3.67TVGFMIKK117 pKa = 10.08DD118 pKa = 3.49ANDD121 pKa = 3.3VQVAEE126 pKa = 4.45SQNVALDD133 pKa = 3.61VTSADD138 pKa = 3.63TNSYY142 pKa = 9.99SFNVGMGTTDD152 pKa = 3.15ATPAAGSYY160 pKa = 9.69SVPVTVAYY168 pKa = 9.49IVNN171 pKa = 3.77

Molecular weight: 17.27 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>sp|A6TG44|RSMG_KLEP7 Ribosomal RNA small subunit methyltransferase G OS=Klebsiella pneumoniae subsp. pneumoniae (strain ATCC 700721 / MGH 78578) OX=272620 GN=rsmG PE=3 SV=1
MM1 pKa = 7.45KK2 pKa = 9.51RR3 pKa = 11.84TFQPSVLKK11 pKa = 10.6RR12 pKa = 11.84NRR14 pKa = 11.84SHH16 pKa = 7.16GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.4NGRR28 pKa = 11.84QVLARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.22GRR39 pKa = 11.84ARR41 pKa = 11.84LTVSKK46 pKa = 10.99

Molecular weight: 5.38 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5126

0

5126

1601607

19

3944

312.4

34.46

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.411 ± 0.045

1.066 ± 0.01

5.189 ± 0.071

5.484 ± 0.031

3.836 ± 0.027

7.578 ± 0.031

2.276 ± 0.016

5.585 ± 0.029

3.837 ± 0.031

11.076 ± 0.048

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.731 ± 0.019

3.41 ± 0.02

4.629 ± 0.026

4.518 ± 0.032

5.959 ± 0.035

5.832 ± 0.074

5.191 ± 0.02

7.082 ± 0.03

1.584 ± 0.014

2.726 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski