Tomato yellow leaf curl Sardinia virus (isolate Spain-1) (TYLCSV)

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Repensiviricetes; Geplafuvirales; Geminiviridae; Begomovirus; Tomato yellow leaf curl virus

Average proteome isoelectric point is 7.44

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>sp|P61512|AV2_TYCS1 Protein V2 OS=Tomato yellow leaf curl Sardinia virus (isolate Spain-1) OX=37139 GN=V2 PE=3 SV=1
MM1 pKa = 8.06WDD3 pKa = 3.35PLLNEE8 pKa = 4.99FPDD11 pKa = 3.91SVHH14 pKa = 6.92GLRR17 pKa = 11.84CMLAIKK23 pKa = 9.84YY24 pKa = 8.5LQLVEE29 pKa = 4.18EE30 pKa = 4.6TYY32 pKa = 10.95EE33 pKa = 4.26PNTLGHH39 pKa = 6.99DD40 pKa = 5.01LIRR43 pKa = 11.84DD44 pKa = 4.58LISVIRR50 pKa = 11.84ARR52 pKa = 11.84DD53 pKa = 3.33YY54 pKa = 11.41AEE56 pKa = 3.94ANRR59 pKa = 11.84RR60 pKa = 11.84YY61 pKa = 8.57TNFNARR67 pKa = 11.84LEE69 pKa = 4.56GSSKK73 pKa = 9.87TEE75 pKa = 3.5LRR77 pKa = 11.84QPVYY81 pKa = 10.47QPCCCPHH88 pKa = 6.81CPRR91 pKa = 11.84HH92 pKa = 5.34QASIMDD98 pKa = 4.25LQAHH102 pKa = 5.55VSKK105 pKa = 10.85AADD108 pKa = 3.44VQNVQKK114 pKa = 10.68PP115 pKa = 3.36

Molecular weight: 13.25 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>sp|P38609|REP_TYCS1 Replication-associated protein OS=Tomato yellow leaf curl Sardinia virus (isolate Spain-1) OX=37139 GN=C1 PE=3 SV=1
MM1 pKa = 7.94PKK3 pKa = 9.0RR4 pKa = 11.84TGDD7 pKa = 3.53ILISTPVSKK16 pKa = 10.63VRR18 pKa = 11.84RR19 pKa = 11.84KK20 pKa = 10.58LNFDD24 pKa = 3.29SPYY27 pKa = 8.76TSRR30 pKa = 11.84AAAPTVQGIKK40 pKa = 9.78RR41 pKa = 11.84RR42 pKa = 11.84SWTYY46 pKa = 9.66RR47 pKa = 11.84PMYY50 pKa = 9.54RR51 pKa = 11.84KK52 pKa = 8.92PRR54 pKa = 11.84MYY56 pKa = 10.82RR57 pKa = 11.84MYY59 pKa = 10.38RR60 pKa = 11.84SPDD63 pKa = 3.25VPFGCEE69 pKa = 4.38GPCKK73 pKa = 9.31VQSYY77 pKa = 6.99EE78 pKa = 3.67QRR80 pKa = 11.84DD81 pKa = 4.07DD82 pKa = 3.72VKK84 pKa = 9.02HH85 pKa = 5.75TGVVRR90 pKa = 11.84CVSDD94 pKa = 3.24VTRR97 pKa = 11.84GSGITHH103 pKa = 6.35RR104 pKa = 11.84VGKK107 pKa = 9.68RR108 pKa = 11.84FCIKK112 pKa = 10.26SIYY115 pKa = 9.81ILGKK119 pKa = 9.09IWMDD123 pKa = 3.64EE124 pKa = 4.09NIKK127 pKa = 10.06KK128 pKa = 9.97QNHH131 pKa = 4.95TNQVMFFLVRR141 pKa = 11.84DD142 pKa = 3.69RR143 pKa = 11.84RR144 pKa = 11.84PYY146 pKa = 8.79GTSPMDD152 pKa = 3.74FGQVFNMFDD161 pKa = 3.99NEE163 pKa = 4.11PSTATVKK170 pKa = 10.81NDD172 pKa = 2.84LRR174 pKa = 11.84DD175 pKa = 3.43RR176 pKa = 11.84YY177 pKa = 9.52QVMRR181 pKa = 11.84KK182 pKa = 7.66FHH184 pKa = 5.81ATVVGGPSGMKK195 pKa = 8.95EE196 pKa = 3.53QCLLKK201 pKa = 10.64RR202 pKa = 11.84FFKK205 pKa = 11.17VNTHH209 pKa = 4.57VVYY212 pKa = 10.83NHH214 pKa = 5.75QEE216 pKa = 3.59QAKK219 pKa = 9.66YY220 pKa = 10.54EE221 pKa = 4.12NHH223 pKa = 6.33TEE225 pKa = 3.99NALLLYY231 pKa = 7.29MACTHH236 pKa = 7.07ASNPVYY242 pKa = 9.86ATLKK246 pKa = 9.47IRR248 pKa = 11.84IYY250 pKa = 10.65FYY252 pKa = 11.26DD253 pKa = 3.34AVTNN257 pKa = 4.16

Molecular weight: 29.99 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6

0

6

1096

96

359

182.7

21.15

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.562 ± 0.843

2.099 ± 0.466

5.474 ± 0.361

4.288 ± 0.585

4.927 ± 0.634

4.38 ± 0.422

4.106 ± 0.569

5.839 ± 0.631

5.474 ± 0.932

7.208 ± 0.816

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.372 ± 0.587

5.931 ± 0.578

5.566 ± 0.49

6.204 ± 0.776

6.569 ± 1.046

7.573 ± 1.106

6.204 ± 0.97

5.748 ± 0.783

1.277 ± 0.241

4.197 ± 0.582
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski