Tobacco vein clearing virus (isolate Nicotiana edwardsonii/United States/Lockhart/2000) (TVCV)
Average proteome isoelectric point is 7.05
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|Q9QD06|Q9QD06_TVCVL Coat protein OS=Tobacco vein clearing virus (isolate Nicotiana edwardsonii/United States/Lockhart/2000) OX=1289468 PE=3 SV=1
MM1 pKa = 7.29 NKK3 pKa = 9.92 EE4 pKa = 3.58 EE5 pKa = 3.95 FAIDD9 pKa = 3.23 EE10 pKa = 4.39 KK11 pKa = 10.6 TYY13 pKa = 10.94 EE14 pKa = 4.17 NQDD17 pKa = 3.09 GLKK20 pKa = 10.16 IKK22 pKa = 10.43 IIFSNLGRR30 pKa = 11.84 RR31 pKa = 11.84 YY32 pKa = 10.28 KK33 pKa = 10.75 KK34 pKa = 10.44 IGDD37 pKa = 3.75 QIYY40 pKa = 11.08 LMIEE44 pKa = 3.89 KK45 pKa = 8.47 EE46 pKa = 4.23 TARR49 pKa = 11.84 LEE51 pKa = 4.41 DD52 pKa = 3.99 SLTAMTRR59 pKa = 11.84 IIKK62 pKa = 10.09 EE63 pKa = 3.74 NEE65 pKa = 3.9 EE66 pKa = 3.59 IDD68 pKa = 3.41 KK69 pKa = 10.88 KK70 pKa = 11.12 RR71 pKa = 11.84 EE72 pKa = 3.47 IEE74 pKa = 4.3 IIRR77 pKa = 11.84 NQANKK82 pKa = 10.06 EE83 pKa = 3.92 IQQLEE88 pKa = 4.2 EE89 pKa = 4.08 TKK91 pKa = 9.21 NTKK94 pKa = 10.06 IIALEE99 pKa = 4.22 KK100 pKa = 9.27 EE101 pKa = 4.24 LNMLKK106 pKa = 10.56 LLYY109 pKa = 10.1 EE110 pKa = 4.21 NKK112 pKa = 9.44 QRR114 pKa = 11.84 EE115 pKa = 4.03 KK116 pKa = 11.04 DD117 pKa = 3.63 KK118 pKa = 11.07 EE119 pKa = 3.95 IEE121 pKa = 4.12 LTDD124 pKa = 4.33 EE125 pKa = 3.92 INKK128 pKa = 9.34 FKK130 pKa = 11.04 QKK132 pKa = 10.41 LQPEE136 pKa = 4.19 EE137 pKa = 3.68 NLRR140 pKa = 11.84 IEE142 pKa = 5.14 EE143 pKa = 4.08 IDD145 pKa = 4.68 NNIDD149 pKa = 3.58 DD150 pKa = 4.38 QKK152 pKa = 11.6 SEE154 pKa = 3.98 LSEE157 pKa = 3.73 ISEE160 pKa = 4.7 TYY162 pKa = 10.27 TEE164 pKa = 3.8 ILEE167 pKa = 4.7 NIEE170 pKa = 3.84 KK171 pKa = 9.9 TKK173 pKa = 10.74 NLEE176 pKa = 3.93 INTGDD181 pKa = 3.23 VNMDD185 pKa = 4.01 EE186 pKa = 4.93 KK187 pKa = 10.97 PSTSGIKK194 pKa = 9.88 NPKK197 pKa = 8.41 EE198 pKa = 4.03 LNPSYY203 pKa = 9.95 YY204 pKa = 9.19 TVSYY208 pKa = 9.33 EE209 pKa = 4.24 QSDD212 pKa = 3.94 RR213 pKa = 11.84 NNTLWNSRR221 pKa = 11.84 LNKK224 pKa = 9.73 KK225 pKa = 6.57 WTPKK229 pKa = 9.99 PIHH232 pKa = 5.59 EE233 pKa = 4.55 QYY235 pKa = 11.56 NFLDD239 pKa = 4.21 LDD241 pKa = 3.99 CVEE244 pKa = 5.83 DD245 pKa = 3.67 INKK248 pKa = 8.83 TIQLWIGYY256 pKa = 9.01 ISKK259 pKa = 10.16 QLIDD263 pKa = 3.65 NKK265 pKa = 10.46 IGITEE270 pKa = 4.03 TPGYY274 pKa = 9.94 IEE276 pKa = 4.09 RR277 pKa = 11.84 TLIGTVKK284 pKa = 10.38 LWLHH288 pKa = 6.09 NLTKK292 pKa = 10.71 EE293 pKa = 4.47 SIDD296 pKa = 3.44 TLRR299 pKa = 11.84 SNKK302 pKa = 9.39 KK303 pKa = 10.28 FNGDD307 pKa = 3.05 MATTSMEE314 pKa = 3.66 ILYY317 pKa = 9.84 KK318 pKa = 10.41 YY319 pKa = 9.98 EE320 pKa = 3.44 IAIRR324 pKa = 11.84 NEE326 pKa = 3.89 FSSMTTEE333 pKa = 3.81 TEE335 pKa = 3.98 EE336 pKa = 3.8 QHH338 pKa = 7.01 KK339 pKa = 10.21 EE340 pKa = 3.64 KK341 pKa = 10.73 QINRR345 pKa = 11.84 NLMTKK350 pKa = 10.48 LAICNMCYY358 pKa = 9.36 IDD360 pKa = 5.37 EE361 pKa = 4.4 YY362 pKa = 9.24 TCAFRR367 pKa = 11.84 EE368 pKa = 4.21 YY369 pKa = 10.12 YY370 pKa = 10.55 YY371 pKa = 10.95 KK372 pKa = 10.25 GTYY375 pKa = 7.49 NTEE378 pKa = 3.68 EE379 pKa = 3.82 GRR381 pKa = 11.84 EE382 pKa = 3.86 IRR384 pKa = 11.84 KK385 pKa = 9.8 LYY387 pKa = 8.11 FTKK390 pKa = 10.57 LPEE393 pKa = 4.44 PFSSKK398 pKa = 10.1 IIKK401 pKa = 9.51 DD402 pKa = 3.25 WNEE405 pKa = 3.56 AGLTDD410 pKa = 3.92 TLGARR415 pKa = 11.84 IKK417 pKa = 10.46 FLQQWFVQLCEE428 pKa = 4.25 KK429 pKa = 10.53 YY430 pKa = 9.85 KK431 pKa = 11.07 EE432 pKa = 4.1 EE433 pKa = 4.01 IKK435 pKa = 10.1 MEE437 pKa = 4.81 KK438 pKa = 9.95 ILIKK442 pKa = 10.5 NLKK445 pKa = 8.3 CCKK448 pKa = 10.33 NKK450 pKa = 8.96 TAPQFGCTDD459 pKa = 3.66 KK460 pKa = 11.55 YY461 pKa = 9.57 NKK463 pKa = 9.24 KK464 pKa = 9.59 WKK466 pKa = 7.12 TKK468 pKa = 9.42 KK469 pKa = 9.74 YY470 pKa = 8.88 KK471 pKa = 9.96 KK472 pKa = 9.68 HH473 pKa = 5.88 RR474 pKa = 11.84 SKK476 pKa = 11.29 YY477 pKa = 9.46 KK478 pKa = 8.9 YY479 pKa = 8.76 KK480 pKa = 10.52 KK481 pKa = 8.41 PRR483 pKa = 11.84 KK484 pKa = 8.48 RR485 pKa = 11.84 YY486 pKa = 8.5 YY487 pKa = 9.67 VKK489 pKa = 10.52 NYY491 pKa = 7.26 QAKK494 pKa = 10.24 KK495 pKa = 9.49 PFRR498 pKa = 11.84 PKK500 pKa = 10.55 KK501 pKa = 10.26 KK502 pKa = 9.27 LTEE505 pKa = 4.2 CTCYY509 pKa = 10.48 NCGKK513 pKa = 9.63 LGHH516 pKa = 6.75 IAKK519 pKa = 9.97 DD520 pKa = 3.64 CKK522 pKa = 10.8 APKK525 pKa = 9.88 NPKK528 pKa = 9.38 KK529 pKa = 10.6 KK530 pKa = 10.33 QITEE534 pKa = 4.1 IIIDD538 pKa = 3.97 DD539 pKa = 4.18 EE540 pKa = 5.3 EE541 pKa = 4.68 YY542 pKa = 10.36 MQMEE546 pKa = 4.74 YY547 pKa = 9.93 IDD549 pKa = 4.54 YY550 pKa = 10.44 EE551 pKa = 4.42 LDD553 pKa = 3.76 SEE555 pKa = 4.4 DD556 pKa = 4.11 SIYY559 pKa = 10.68 EE560 pKa = 3.79 ISDD563 pKa = 3.3 SDD565 pKa = 4.76 DD566 pKa = 4.7 EE567 pKa = 5.01 IDD569 pKa = 4.38 SEE571 pKa = 4.22 ITEE574 pKa = 4.4 EE575 pKa = 3.96 EE576 pKa = 4.37 DD577 pKa = 4.17 EE578 pKa = 4.27 II579 pKa = 6.24
Molecular weight: 69.08 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.675
IPC2_protein 5.931
IPC_protein 5.906
Toseland 5.944
ProMoST 6.135
Dawson 6.02
Bjellqvist 6.084
Wikipedia 5.944
Rodwell 5.982
Grimsley 5.944
Solomon 6.02
Lehninger 5.995
Nozaki 6.211
DTASelect 6.351
Thurlkill 6.262
EMBOSS 6.198
Sillero 6.313
Patrickios 4.279
IPC_peptide 6.033
IPC2_peptide 6.186
IPC2.peptide.svr19 6.52
Protein with the highest isoelectric point:
>tr|Q9QD05|Q9QD05_TVCVL Putative cell-to-cell movement protein OS=Tobacco vein clearing virus (isolate Nicotiana edwardsonii/United States/Lockhart/2000) OX=1289468 PE=4 SV=1
MM1 pKa = 7.76 KK2 pKa = 9.95 IYY4 pKa = 10.16 ILAKK8 pKa = 9.84 IIVEE12 pKa = 4.68 GYY14 pKa = 7.64 YY15 pKa = 10.52 NRR17 pKa = 11.84 YY18 pKa = 5.62 YY19 pKa = 10.91 TPMIDD24 pKa = 3.21 TGAEE28 pKa = 3.94 ANICKK33 pKa = 10.01 YY34 pKa = 10.96 NCLPTDD40 pKa = 2.7 KK41 pKa = 10.12 WEE43 pKa = 4.11 KK44 pKa = 10.57 LKK46 pKa = 10.39 TPMVVTGFNNEE57 pKa = 3.55 GSMINYY63 pKa = 8.05 KK64 pKa = 10.3 ARR66 pKa = 11.84 NVKK69 pKa = 9.16 IQIWDD74 pKa = 3.82 KK75 pKa = 10.56 ILTIEE80 pKa = 4.47 EE81 pKa = 4.27 IYY83 pKa = 11.11 NFEE86 pKa = 4.19 FTTKK90 pKa = 10.94 DD91 pKa = 3.28 MLLGMPFLEE100 pKa = 4.44 KK101 pKa = 10.31 LYY103 pKa = 10.18 PHH105 pKa = 7.77 IITKK109 pKa = 5.99 THH111 pKa = 4.35 WWFTTPCKK119 pKa = 10.68 NKK121 pKa = 10.06 VGAKK125 pKa = 9.31 RR126 pKa = 11.84 VNNKK130 pKa = 7.37 QRR132 pKa = 11.84 KK133 pKa = 4.02 TTEE136 pKa = 3.99 WIRR139 pKa = 11.84 GSEE142 pKa = 4.29 KK143 pKa = 9.4 ITQKK147 pKa = 11.04 LEE149 pKa = 4.36 NINKK153 pKa = 7.77 NTTTQLEE160 pKa = 4.48 IIIFTIDD167 pKa = 3.05 KK168 pKa = 10.8 VKK170 pKa = 10.49 IIQKK174 pKa = 10.36 KK175 pKa = 9.55 LEE177 pKa = 3.98 KK178 pKa = 10.37 LYY180 pKa = 11.06 NDD182 pKa = 4.09 NPLQGWEE189 pKa = 3.85 KK190 pKa = 10.67 HH191 pKa = 4.0 KK192 pKa = 9.91 TKK194 pKa = 10.89 VKK196 pKa = 10.4 IEE198 pKa = 4.45 LIEE201 pKa = 4.25 EE202 pKa = 3.95 NSIITQKK209 pKa = 9.58 PLKK212 pKa = 10.73 YY213 pKa = 10.34 NFNDD217 pKa = 3.07 LTEE220 pKa = 4.39 FKK222 pKa = 10.57 IHH224 pKa = 7.21 IKK226 pKa = 10.67 DD227 pKa = 3.75 LLDD230 pKa = 3.42 NKK232 pKa = 10.22 YY233 pKa = 10.03 IQEE236 pKa = 4.38 SNSKK240 pKa = 8.07 HH241 pKa = 5.22 TSPAFIVNKK250 pKa = 10.11 HH251 pKa = 5.18 SEE253 pKa = 4.14 QKK255 pKa = 9.79 RR256 pKa = 11.84 GKK258 pKa = 9.08 SRR260 pKa = 11.84 MVIDD264 pKa = 4.04 YY265 pKa = 10.56 RR266 pKa = 11.84 NLNAKK271 pKa = 7.98 TKK273 pKa = 8.87 TYY275 pKa = 10.75 NYY277 pKa = 7.77 PIPNKK282 pKa = 9.55 ILKK285 pKa = 9.19 IRR287 pKa = 11.84 QIQGYY292 pKa = 9.56 NYY294 pKa = 9.96 FSKK297 pKa = 10.61 FDD299 pKa = 4.02 CKK301 pKa = 10.9 SGFYY305 pKa = 10.02 HH306 pKa = 7.35 LKK308 pKa = 10.93 LEE310 pKa = 4.62 DD311 pKa = 3.65 EE312 pKa = 4.66 SKK314 pKa = 11.06 KK315 pKa = 9.77 LTAFTVPQGFYY326 pKa = 9.41 EE327 pKa = 4.28 WNVLPFGYY335 pKa = 10.23 KK336 pKa = 9.53 NAPGRR341 pKa = 11.84 YY342 pKa = 6.04 QHH344 pKa = 6.91 FMDD347 pKa = 4.7 NYY349 pKa = 9.34 FNQLEE354 pKa = 4.15 NCIVYY359 pKa = 10.02 IDD361 pKa = 5.64 DD362 pKa = 3.36 ILLYY366 pKa = 10.93 SRR368 pKa = 11.84 TQDD371 pKa = 3.14 EE372 pKa = 4.81 HH373 pKa = 7.93 IKK375 pKa = 10.58 LLEE378 pKa = 4.27 KK379 pKa = 10.0 FAHH382 pKa = 6.54 IIEE385 pKa = 4.4 NSGISLSKK393 pKa = 9.83 TKK395 pKa = 11.0 AEE397 pKa = 3.73 IMKK400 pKa = 10.06 NQIEE404 pKa = 4.17 FLGIQIDD411 pKa = 3.82 KK412 pKa = 11.11 NGIKK416 pKa = 8.84 MQTHH420 pKa = 6.44 IVQKK424 pKa = 10.46 IINLDD429 pKa = 3.6 EE430 pKa = 5.32 NIDD433 pKa = 3.95 TKK435 pKa = 11.26 KK436 pKa = 10.64 KK437 pKa = 9.4 LQSFLGIVNQVRR449 pKa = 11.84 EE450 pKa = 4.78 YY451 pKa = 10.52 IPKK454 pKa = 9.98 LAEE457 pKa = 3.88 NLKK460 pKa = 10.03 PLQKK464 pKa = 10.38 KK465 pKa = 9.34 LKK467 pKa = 10.02 KK468 pKa = 10.1 DD469 pKa = 3.28 VEE471 pKa = 4.3 YY472 pKa = 11.37 SFDD475 pKa = 3.92 EE476 pKa = 4.36 KK477 pKa = 11.22 DD478 pKa = 3.05 KK479 pKa = 11.27 EE480 pKa = 4.13 QIKK483 pKa = 10.16 KK484 pKa = 10.37 IKK486 pKa = 9.72 ILCKK490 pKa = 10.23 KK491 pKa = 10.06 LPKK494 pKa = 10.31 LYY496 pKa = 10.72 FPDD499 pKa = 3.63 EE500 pKa = 4.39 NKK502 pKa = 10.17 KK503 pKa = 8.68 FTYY506 pKa = 9.92 IVEE509 pKa = 4.44 TDD511 pKa = 3.28 SSNYY515 pKa = 10.37 SYY517 pKa = 11.63 GGVLKK522 pKa = 10.7 YY523 pKa = 10.35 RR524 pKa = 11.84 YY525 pKa = 8.69 NKK527 pKa = 9.98 EE528 pKa = 4.13 KK529 pKa = 10.28 IEE531 pKa = 3.99 HH532 pKa = 5.78 HH533 pKa = 6.1 CRR535 pKa = 11.84 YY536 pKa = 10.05 YY537 pKa = 10.68 SGSYY541 pKa = 9.4 TEE543 pKa = 4.76 PQEE546 pKa = 3.65 KK547 pKa = 9.45 WEE549 pKa = 4.18 INRR552 pKa = 11.84 KK553 pKa = 8.0 EE554 pKa = 4.06 LFALYY559 pKa = 10.07 KK560 pKa = 10.4 CLLAFEE566 pKa = 4.93 PYY568 pKa = 9.5 IVYY571 pKa = 9.05 TRR573 pKa = 11.84 FIVRR577 pKa = 11.84 TDD579 pKa = 3.16 NTQVKK584 pKa = 7.71 WWITRR589 pKa = 11.84 KK590 pKa = 9.67 VQDD593 pKa = 3.37 SVTTKK598 pKa = 10.13 EE599 pKa = 3.62 IRR601 pKa = 11.84 RR602 pKa = 11.84 LVLNILNFTFTIEE615 pKa = 4.96 IINTNKK621 pKa = 10.13 NVVADD626 pKa = 3.77 YY627 pKa = 10.86 LSRR630 pKa = 11.84 QSYY633 pKa = 9.84 PNN635 pKa = 3.3
Molecular weight: 75.69 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.782
IPC2_protein 8.843
IPC_protein 8.668
Toseland 9.458
ProMoST 9.209
Dawson 9.721
Bjellqvist 9.428
Wikipedia 9.897
Rodwell 10.087
Grimsley 9.809
Solomon 9.736
Lehninger 9.692
Nozaki 9.516
DTASelect 9.399
Thurlkill 9.56
EMBOSS 9.882
Sillero 9.648
Patrickios 4.8
IPC_peptide 9.736
IPC2_peptide 7.907
IPC2.peptide.svr19 7.894
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4
0
4
2050
417
635
512.5
60.56
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
3.024 ± 0.387
1.463 ± 0.209
4.439 ± 0.499
9.902 ± 1.288
2.927 ± 0.535
3.073 ± 0.21
1.561 ± 0.298
11.61 ± 1.333
12.146 ± 1.178
7.659 ± 0.413
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.805 ± 0.272
7.317 ± 0.347
3.366 ± 0.313
4.39 ± 0.437
3.805 ± 0.231
4.683 ± 0.439
7.024 ± 0.486
3.073 ± 0.686
1.073 ± 0.223
5.659 ± 0.26
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here