Entercoccus phage phiM1EF2

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Saphexavirus; unclassified Saphexavirus

Average proteome isoelectric point is 5.7

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 91 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6J4D376|A0A6J4D376_9CAUD Uncharacterized protein OS=Entercoccus phage phiM1EF2 OX=2735669 GN=phiM1EF2_052 PE=4 SV=1
MM1 pKa = 7.23TKK3 pKa = 10.39EE4 pKa = 3.85NNVFLNEE11 pKa = 4.1KK12 pKa = 10.55GFFDD16 pKa = 5.35KK17 pKa = 10.65IIEE20 pKa = 4.14VLEE23 pKa = 4.06NGYY26 pKa = 8.09TGYY29 pKa = 11.24YY30 pKa = 9.38CDD32 pKa = 3.56LHH34 pKa = 8.65GEE36 pKa = 4.02IFNYY40 pKa = 9.64GVNADD45 pKa = 3.41IKK47 pKa = 10.74DD48 pKa = 3.66LEE50 pKa = 4.45EE51 pKa = 4.4YY52 pKa = 11.03GVFNALGEE60 pKa = 4.03IQEE63 pKa = 4.24YY64 pKa = 10.02EE65 pKa = 3.98KK66 pKa = 11.43EE67 pKa = 4.22NFGEE71 pKa = 4.19VLTDD75 pKa = 3.99LGNASAVADD84 pKa = 3.54MLYY87 pKa = 10.25YY88 pKa = 10.12IKK90 pKa = 10.97GEE92 pKa = 4.04EE93 pKa = 4.1FLLEE97 pKa = 3.91TLNFNRR103 pKa = 11.84VLEE106 pKa = 4.27EE107 pKa = 3.69VSEE110 pKa = 4.47DD111 pKa = 3.53FFGNTDD117 pKa = 3.09LWNEE121 pKa = 4.06QAEE124 pKa = 4.36EE125 pKa = 4.53EE126 pKa = 4.41YY127 pKa = 11.34NKK129 pKa = 10.91AIVNRR134 pKa = 11.84LLEE137 pKa = 4.31EE138 pKa = 4.18YY139 pKa = 10.95KK140 pKa = 10.77NVYY143 pKa = 9.09CC144 pKa = 5.99

Molecular weight: 16.8 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6J4D230|A0A6J4D230_9CAUD Uncharacterized protein OS=Entercoccus phage phiM1EF2 OX=2735669 GN=phiM1EF2_005 PE=4 SV=1
MM1 pKa = 7.12GRR3 pKa = 11.84AGGSFSEE10 pKa = 4.72GGRR13 pKa = 11.84GARR16 pKa = 11.84QSASDD21 pKa = 3.65KK22 pKa = 9.56FWEE25 pKa = 4.06IRR27 pKa = 11.84HH28 pKa = 5.43YY29 pKa = 10.65RR30 pKa = 11.84LPIYY34 pKa = 9.93IFPKK38 pKa = 9.86ILYY41 pKa = 8.51KK42 pKa = 10.67ASRR45 pKa = 11.84QLPDD49 pKa = 3.19SYY51 pKa = 11.52SDD53 pKa = 3.73KK54 pKa = 10.85LSEE57 pKa = 5.38AIPTAIRR64 pKa = 3.41

Molecular weight: 7.25 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

91

0

91

16567

45

1330

182.1

20.65

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.249 ± 0.439

0.664 ± 0.088

6.139 ± 0.2

8.964 ± 0.392

4.28 ± 0.202

6.151 ± 0.364

1.431 ± 0.121

6.386 ± 0.226

7.612 ± 0.271

8.553 ± 0.319

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.68 ± 0.165

5.179 ± 0.188

2.946 ± 0.238

3.495 ± 0.235

4.056 ± 0.217

5.946 ± 0.201

6.096 ± 0.313

6.513 ± 0.241

1.31 ± 0.113

4.352 ± 0.242
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski