Exidia glandulosa HHB12029
Average proteome isoelectric point is 6.76
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 26313 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A165P2Z6|A0A165P2Z6_EXIGL Uncharacterized protein OS=Exidia glandulosa HHB12029 OX=1314781 GN=EXIGLDRAFT_65935 PE=4 SV=1
MM1 pKa = 7.7 RR2 pKa = 11.84 FSVLLVAAAATLVSAQTSSSLNNLFGRR29 pKa = 11.84 ATDD32 pKa = 3.99 TSACASDD39 pKa = 5.15 CGAQSAAANLDD50 pKa = 3.37 AATKK54 pKa = 9.08 CTTDD58 pKa = 3.2 SGGDD62 pKa = 3.5 STKK65 pKa = 10.98 LQICVCQSPALVQGVNDD82 pKa = 4.42 CMQKK86 pKa = 9.99 EE87 pKa = 4.64 CPDD90 pKa = 3.95 DD91 pKa = 3.51 AHH93 pKa = 6.7 FFQDD97 pKa = 3.04 GCDD100 pKa = 3.4 IITGKK105 pKa = 9.14 TSTTGSDD112 pKa = 3.54 PASTPSDD119 pKa = 3.73 SASTPSDD126 pKa = 3.54 SASTPTDD133 pKa = 3.24 SDD135 pKa = 3.9 SASTPTASGTGSAGSLGVSAGVALSSVLFAGVALLL170 pKa = 4.44
Molecular weight: 16.59 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.697
IPC2_protein 3.795
IPC_protein 3.795
Toseland 3.554
ProMoST 4.012
Dawson 3.834
Bjellqvist 3.986
Wikipedia 3.846
Rodwell 3.63
Grimsley 3.465
Solomon 3.821
Lehninger 3.77
Nozaki 3.961
DTASelect 4.291
Thurlkill 3.643
EMBOSS 3.846
Sillero 3.935
Patrickios 0.896
IPC_peptide 3.808
IPC2_peptide 3.897
IPC2.peptide.svr19 3.823
Protein with the highest isoelectric point:
>tr|A0A165QPS0|A0A165QPS0_EXIGL SecE/sec61-gamma protein OS=Exidia glandulosa HHB12029 OX=1314781 GN=EXIGLDRAFT_715939 PE=3 SV=1
MM1 pKa = 7.38 NLTYY5 pKa = 8.06 PTTLHH10 pKa = 5.07 AHH12 pKa = 6.06 IMFGHH17 pKa = 6.18 TSRR20 pKa = 11.84 SRR22 pKa = 11.84 STRR25 pKa = 11.84 FASTRR30 pKa = 11.84 RR31 pKa = 11.84 SRR33 pKa = 11.84 RR34 pKa = 11.84 NPSLRR39 pKa = 11.84 VPAVRR44 pKa = 11.84 RR45 pKa = 11.84 SKK47 pKa = 9.72 PTFGQRR53 pKa = 11.84 IKK55 pKa = 10.83 AALGQRR61 pKa = 11.84 PRR63 pKa = 11.84 TAGRR67 pKa = 11.84 SSRR70 pKa = 11.84 TGNRR74 pKa = 11.84 HH75 pKa = 3.12 VTLVRR80 pKa = 11.84 RR81 pKa = 11.84 GNRR84 pKa = 11.84 THH86 pKa = 6.1 RR87 pKa = 11.84 TVVPSRR93 pKa = 11.84 SKK95 pKa = 10.25 PSLAARR101 pKa = 11.84 IKK103 pKa = 10.68 RR104 pKa = 11.84 LFNVPLSPNAQRR116 pKa = 11.84 SSTRR120 pKa = 11.84 RR121 pKa = 11.84 RR122 pKa = 11.84 MHH124 pKa = 6.23 AA125 pKa = 3.29
Molecular weight: 14.27 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.526
IPC2_protein 11.462
IPC_protein 12.939
Toseland 13.1
ProMoST 13.598
Dawson 13.1
Bjellqvist 13.1
Wikipedia 13.583
Rodwell 12.661
Grimsley 13.144
Solomon 13.598
Lehninger 13.495
Nozaki 13.1
DTASelect 13.1
Thurlkill 13.1
EMBOSS 13.598
Sillero 13.1
Patrickios 12.369
IPC_peptide 13.598
IPC2_peptide 12.588
IPC2.peptide.svr19 9.279
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
26305
8
26313
9701046
49
4819
368.7
40.59
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
10.084 ± 0.015
1.455 ± 0.008
5.824 ± 0.011
5.308 ± 0.015
3.679 ± 0.01
6.305 ± 0.015
2.65 ± 0.007
4.252 ± 0.01
3.69 ± 0.016
9.59 ± 0.02
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.996 ± 0.006
2.954 ± 0.009
6.682 ± 0.019
3.509 ± 0.01
6.962 ± 0.016
8.045 ± 0.018
6.154 ± 0.013
6.815 ± 0.012
1.543 ± 0.006
2.505 ± 0.008
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here