Salmo salar (Atlantic salmon)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Deuterostomia; Chordata; Craniata; Vertebrata; Gnathostomata; Teleostomi; Euteleostomi; Actinopterygii; Actinopteri; Neopterygii; Teleostei; Osteoglossocephalai; Clupeocephala; Euteleosteomorpha; Protacanthopterygii; Salmoniformes; Salmonidae; Salmoninae;

Average proteome isoelectric point is 6.46

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 82392 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1S3R2G4|A0A1S3R2G4_SALSA dual specificity testis-specific protein kinase 2-like OS=Salmo salar OX=8030 GN=LOC106599373 PE=4 SV=1
MM1 pKa = 7.69LLAQINRR8 pKa = 11.84DD9 pKa = 3.41SQGIAEE15 pKa = 4.33FQNAGGDD22 pKa = 3.94SQQVTLCLAEE32 pKa = 4.3AVSDD36 pKa = 3.91GDD38 pKa = 3.92QMDD41 pKa = 4.02MDD43 pKa = 4.6TVSLQAVTLADD54 pKa = 3.56GSTAYY59 pKa = 9.46IQHH62 pKa = 6.13NPKK65 pKa = 10.24DD66 pKa = 4.06GKK68 pKa = 11.24FMDD71 pKa = 4.28GQVIQLEE78 pKa = 4.65DD79 pKa = 3.43GSTAYY84 pKa = 9.69VQHH87 pKa = 7.41VSMPRR92 pKa = 11.84AEE94 pKa = 4.32EE95 pKa = 3.89ANRR98 pKa = 11.84WTGSPLCDD106 pKa = 3.76VIGGDD111 pKa = 4.44GLRR114 pKa = 11.84LEE116 pKa = 5.6DD117 pKa = 3.91GQAVQLEE124 pKa = 4.74DD125 pKa = 3.15GTTAYY130 pKa = 9.77IHH132 pKa = 5.91TPKK135 pKa = 9.87DD136 pKa = 3.23TYY138 pKa = 11.28DD139 pKa = 3.45QNTLQAVQLEE149 pKa = 4.79DD150 pKa = 3.18GTTAYY155 pKa = 9.44IQHH158 pKa = 6.15TVQMPQTNTILAIQADD174 pKa = 4.42GTVSDD179 pKa = 5.05LNADD183 pKa = 3.55GTLDD187 pKa = 3.46QEE189 pKa = 4.81TINVLEE195 pKa = 4.12QYY197 pKa = 7.13TAKK200 pKa = 10.14VVCYY204 pKa = 10.39

Molecular weight: 22.02 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1S3MIX3|A0A1S3MIX3_SALSA RNA-binding protein 10-like OS=Salmo salar OX=8030 GN=LOC106572966 PE=4 SV=1
MM1 pKa = 7.41FLLRR5 pKa = 11.84HH6 pKa = 5.69QLTTHH11 pKa = 5.94TPIHH15 pKa = 5.95RR16 pKa = 11.84HH17 pKa = 4.92QLTTHH22 pKa = 5.9TPIHH26 pKa = 6.08RR27 pKa = 11.84HH28 pKa = 4.92QLTISHH34 pKa = 6.18TPIHH38 pKa = 6.21RR39 pKa = 11.84HH40 pKa = 4.95QLTISHH46 pKa = 6.04TLIHH50 pKa = 6.38RR51 pKa = 11.84HH52 pKa = 5.42QLTTHH57 pKa = 5.9TPIHH61 pKa = 5.95RR62 pKa = 11.84HH63 pKa = 4.92QLTTHH68 pKa = 5.9TPIHH72 pKa = 6.08RR73 pKa = 11.84HH74 pKa = 4.92QLTISHH80 pKa = 6.04TLIHH84 pKa = 6.46RR85 pKa = 11.84HH86 pKa = 5.33QLTISHH92 pKa = 6.04TLIHH96 pKa = 6.46RR97 pKa = 11.84HH98 pKa = 5.32QLTISHH104 pKa = 6.8ILIFRR109 pKa = 11.84HH110 pKa = 5.49QLTISHH116 pKa = 6.04TLIHH120 pKa = 6.38RR121 pKa = 11.84HH122 pKa = 5.42QLTTHH127 pKa = 5.9TPIHH131 pKa = 5.95RR132 pKa = 11.84HH133 pKa = 4.92QLTTHH138 pKa = 5.9TPIHH142 pKa = 6.08RR143 pKa = 11.84HH144 pKa = 4.87QLTITHH150 pKa = 6.75SNTQASTNHH159 pKa = 5.29FTHH162 pKa = 6.94SNTQASTNHH171 pKa = 5.29FTHH174 pKa = 6.94SNTQASTNHH183 pKa = 5.29FTHH186 pKa = 6.94SNTQASTNHH195 pKa = 5.29FTHH198 pKa = 6.97SNTQTSTNHH207 pKa = 5.54LTHH210 pKa = 7.2SITQASTNHH219 pKa = 5.0THH221 pKa = 7.35SNTQTSTNHH230 pKa = 4.57THH232 pKa = 7.03SNTQASTNHH241 pKa = 5.29FTHH244 pKa = 6.94SNTQASTNHH253 pKa = 5.29FTHH256 pKa = 6.94SNTQASTNHH265 pKa = 5.28FTHH268 pKa = 7.2SITQASTNHH277 pKa = 5.26FTHH280 pKa = 6.94SNTQASTKK288 pKa = 10.36HH289 pKa = 4.83FTHH292 pKa = 6.94SNTQASTNHH301 pKa = 5.29FTHH304 pKa = 7.11SNTQTT309 pKa = 3.08

Molecular weight: 35.59 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

47781

34611

82392

58348648

31

32943

708.2

78.71

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.422 ± 0.008

2.055 ± 0.007

5.215 ± 0.006

7.144 ± 0.015

3.16 ± 0.006

6.76 ± 0.014

2.711 ± 0.006

4.077 ± 0.009

5.541 ± 0.013

9.125 ± 0.013

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.458 ± 0.005

3.722 ± 0.005

6.241 ± 0.014

5.009 ± 0.012

5.722 ± 0.007

8.847 ± 0.012

5.928 ± 0.01

6.215 ± 0.009

1.057 ± 0.003

2.591 ± 0.005
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski