Hymenobacter roseosalivarius DSM 11622

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Cytophagia; Cytophagales; Hymenobacteraceae; Hymenobacter; Hymenobacter roseosalivarius

Average proteome isoelectric point is 6.95

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4391 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1W1UTU5|A0A1W1UTU5_9BACT Uncharacterized protein OS=Hymenobacter roseosalivarius DSM 11622 OX=645990 GN=SAMN00120144_0846 PE=4 SV=1
MM1 pKa = 7.47EE2 pKa = 5.42RR3 pKa = 11.84LIDD6 pKa = 4.46ALADD10 pKa = 3.86DD11 pKa = 4.83ADD13 pKa = 4.09FFVEE17 pKa = 4.74HH18 pKa = 5.67VQITAIVFDD27 pKa = 3.87TTDD30 pKa = 4.33DD31 pKa = 3.7VTVWATTYY39 pKa = 10.82FDD41 pKa = 3.99NNLHH45 pKa = 6.2FFHH48 pKa = 7.29LGLQFPALDD57 pKa = 3.85LLLRR61 pKa = 11.84LAGEE65 pKa = 4.08RR66 pKa = 11.84AEE68 pKa = 4.28ALQEE72 pKa = 4.02EE73 pKa = 5.03VAEE76 pKa = 4.25ALATVTEE83 pKa = 4.86WPCLLEE89 pKa = 4.03YY90 pKa = 8.58TTEE93 pKa = 4.11EE94 pKa = 4.41KK95 pKa = 10.85PPVPLPNVAMKK106 pKa = 9.54LACTYY111 pKa = 8.93PATLVEE117 pKa = 5.53DD118 pKa = 4.49DD119 pKa = 5.24DD120 pKa = 4.4EE121 pKa = 7.06GEE123 pKa = 4.18AEE125 pKa = 4.03EE126 pKa = 6.01GEE128 pKa = 4.32DD129 pKa = 4.12EE130 pKa = 5.16EE131 pKa = 5.38EE132 pKa = 5.34SEE134 pKa = 5.97DD135 pKa = 4.42DD136 pKa = 4.0DD137 pKa = 6.02EE138 pKa = 6.12EE139 pKa = 4.32IAPHH143 pKa = 6.41NIFYY147 pKa = 11.12LEE149 pKa = 3.92GVYY152 pKa = 10.59LRR154 pKa = 11.84LEE156 pKa = 4.11PP157 pKa = 4.96

Molecular weight: 17.78 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1W1V3M9|A0A1W1V3M9_9BACT TonB-dependent receptor plug OS=Hymenobacter roseosalivarius DSM 11622 OX=645990 GN=SAMN00120144_1386 PE=4 SV=1
MM1 pKa = 6.27MTSVLIVSGVGRR13 pKa = 11.84AWALHH18 pKa = 4.46IRR20 pKa = 11.84QRR22 pKa = 11.84RR23 pKa = 11.84QRR25 pKa = 11.84KK26 pKa = 8.13GKK28 pKa = 9.52PIFSKK33 pKa = 9.28QSRR36 pKa = 11.84RR37 pKa = 11.84IVRR40 pKa = 11.84IFRR43 pKa = 11.84RR44 pKa = 11.84FGMPGIAFLTPILFSPIGGTVIATQLHH71 pKa = 5.31VPRR74 pKa = 11.84SRR76 pKa = 11.84ILLHH80 pKa = 5.85MLWSAALWGVALTFMTIKK98 pKa = 10.55FSHH101 pKa = 6.57LPIFHH106 pKa = 6.99HH107 pKa = 7.02

Molecular weight: 12.27 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4391

0

4391

1404893

30

2462

319.9

35.38

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.033 ± 0.041

0.743 ± 0.013

4.913 ± 0.027

5.429 ± 0.039

4.203 ± 0.024

7.411 ± 0.037

2.022 ± 0.02

4.774 ± 0.034

4.204 ± 0.038

10.95 ± 0.055

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.962 ± 0.019

3.852 ± 0.035

5.134 ± 0.029

4.692 ± 0.028

6.106 ± 0.033

5.582 ± 0.029

6.196 ± 0.034

7.102 ± 0.031

1.201 ± 0.015

3.489 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski