Helicoverpa armigera granulovirus
Average proteome isoelectric point is 6.55
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 179 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A9YMU9|A9YMU9_9BBAC Chitin-binding type-2 domain-containing protein OS=Helicoverpa armigera granulovirus OX=489830 PE=4 SV=1
MM1 pKa = 7.36 FLLVLIIIVVVVIIGVVLTSNSGDD25 pKa = 3.38 KK26 pKa = 11.07 NNEE29 pKa = 3.71 NFEE32 pKa = 4.16 EE33 pKa = 4.22 EE34 pKa = 4.15 DD35 pKa = 3.91 NKK37 pKa = 10.74 FKK39 pKa = 10.97 LCPPGVVGFVADD51 pKa = 3.98 PTNCSSFYY59 pKa = 10.1 FCPIEE64 pKa = 4.95 LYY66 pKa = 10.4 HH67 pKa = 6.82 LCVDD71 pKa = 4.22 GKK73 pKa = 11.07 VFDD76 pKa = 4.83 IEE78 pKa = 5.92 SEE80 pKa = 4.17 TCMDD84 pKa = 4.94 KK85 pKa = 11.29 NVIDD89 pKa = 4.64 CGDD92 pKa = 3.61 RR93 pKa = 11.84 PYY95 pKa = 10.7 TPPPII100 pKa = 4.83
Molecular weight: 11.11 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.939
IPC2_protein 4.164
IPC_protein 4.062
Toseland 3.872
ProMoST 4.215
Dawson 4.037
Bjellqvist 4.19
Wikipedia 3.961
Rodwell 3.897
Grimsley 3.783
Solomon 4.024
Lehninger 3.986
Nozaki 4.164
DTASelect 4.342
Thurlkill 3.923
EMBOSS 3.973
Sillero 4.177
Patrickios 0.604
IPC_peptide 4.024
IPC2_peptide 4.164
IPC2.peptide.svr19 4.068
Protein with the highest isoelectric point:
>tr|A9YMT7|A9YMT7_9BBAC 38K OS=Helicoverpa armigera granulovirus OX=489830 PE=4 SV=1
MM1 pKa = 7.42 EE2 pKa = 3.98 RR3 pKa = 11.84 TGRR6 pKa = 11.84 RR7 pKa = 11.84 RR8 pKa = 11.84 SRR10 pKa = 11.84 SRR12 pKa = 11.84 SRR14 pKa = 11.84 SPQRR18 pKa = 11.84 RR19 pKa = 11.84 RR20 pKa = 11.84 SYY22 pKa = 10.49 RR23 pKa = 11.84 RR24 pKa = 11.84 RR25 pKa = 11.84 SRR27 pKa = 11.84 STSTGARR34 pKa = 11.84 RR35 pKa = 11.84 RR36 pKa = 11.84 SRR38 pKa = 11.84 SGYY41 pKa = 8.58 RR42 pKa = 11.84 RR43 pKa = 11.84 RR44 pKa = 11.84 SRR46 pKa = 11.84 SGQRR50 pKa = 11.84 RR51 pKa = 11.84 NHH53 pKa = 5.14 VNQYY57 pKa = 7.98 VV58 pKa = 3.01
Molecular weight: 7.21 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.382
IPC2_protein 11.096
IPC_protein 12.427
Toseland 12.574
ProMoST 13.086
Dawson 12.574
Bjellqvist 12.574
Wikipedia 13.056
Rodwell 12.076
Grimsley 12.618
Solomon 13.086
Lehninger 12.983
Nozaki 12.574
DTASelect 12.574
Thurlkill 12.574
EMBOSS 13.086
Sillero 12.574
Patrickios 11.798
IPC_peptide 13.086
IPC2_peptide 12.076
IPC2.peptide.svr19 9.14
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
179
0
179
50360
53
1279
281.3
32.45
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
4.809 ± 0.127
2.421 ± 0.126
6.541 ± 0.125
5.447 ± 0.149
4.426 ± 0.122
3.685 ± 0.148
2.361 ± 0.088
5.866 ± 0.125
6.765 ± 0.23
9.396 ± 0.147
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.575 ± 0.094
6.91 ± 0.14
3.884 ± 0.127
3.898 ± 0.125
4.879 ± 0.151
6.35 ± 0.159
6.267 ± 0.158
7.46 ± 0.137
0.969 ± 0.053
5.089 ± 0.144
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here