Salmonella phage 34

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Podoviridae; Lederbergvirus; Salmonella virus P22

Average proteome isoelectric point is 6.94

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 78 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0N7CFD2|A0A0N7CFD2_BPP22 Uncharacterized protein OS=Salmonella phage 34 OX=1654887 GN=SP34_60 PE=4 SV=1
MM1 pKa = 7.81KK2 pKa = 10.33LRR4 pKa = 11.84VWHH7 pKa = 6.62IPQVPMKK14 pKa = 9.66PFIVEE19 pKa = 3.89VGSVEE24 pKa = 3.92EE25 pKa = 4.47GVRR28 pKa = 11.84MMDD31 pKa = 4.09ALADD35 pKa = 3.83YY36 pKa = 10.94DD37 pKa = 4.43AFQYY41 pKa = 11.41DD42 pKa = 4.1NNIKK46 pKa = 10.06PDD48 pKa = 3.47YY49 pKa = 10.48CNANGLQMFDD59 pKa = 4.55EE60 pKa = 4.74SLTDD64 pKa = 3.69QDD66 pKa = 5.0LEE68 pKa = 5.09DD69 pKa = 4.43MEE71 pKa = 6.96LDD73 pKa = 4.23DD74 pKa = 6.45RR75 pKa = 11.84WIDD78 pKa = 3.34WYY80 pKa = 10.77SEE82 pKa = 4.16CQCYY86 pKa = 10.36DD87 pKa = 3.26DD88 pKa = 3.95PRR90 pKa = 11.84EE91 pKa = 3.93YY92 pKa = 11.13LEE94 pKa = 4.09SLKK97 pKa = 11.11EE98 pKa = 3.88EE99 pKa = 4.04TTAAA103 pKa = 3.74

Molecular weight: 12.18 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0N7CAJ0|A0A0N7CAJ0_BPP22 Na(+)/H(+) antiporter OS=Salmonella phage 34 OX=1654887 GN=SP34_53 PE=4 SV=1
MM1 pKa = 7.22TVITYY6 pKa = 9.11GKK8 pKa = 8.68STFAGNAKK16 pKa = 7.83TRR18 pKa = 11.84RR19 pKa = 11.84HH20 pKa = 4.22EE21 pKa = 4.05RR22 pKa = 11.84RR23 pKa = 11.84RR24 pKa = 11.84KK25 pKa = 9.4LAIEE29 pKa = 4.65RR30 pKa = 11.84DD31 pKa = 4.25TICNIIDD38 pKa = 4.06SIFGCDD44 pKa = 4.43APDD47 pKa = 3.67ASQEE51 pKa = 4.09VKK53 pKa = 10.42AKK55 pKa = 10.57RR56 pKa = 11.84IDD58 pKa = 3.69RR59 pKa = 11.84VTKK62 pKa = 10.39AISLAGTRR70 pKa = 11.84QKK72 pKa = 10.59EE73 pKa = 4.24VEE75 pKa = 4.36GGSVLLPGVALYY87 pKa = 10.51AAGHH91 pKa = 6.21RR92 pKa = 11.84KK93 pKa = 9.32SKK95 pKa = 10.8QITARR100 pKa = 3.53

Molecular weight: 10.97 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

78

0

78

11873

38

725

152.2

17.0

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.458 ± 0.501

1.297 ± 0.161

5.887 ± 0.277

6.57 ± 0.35

3.361 ± 0.239

6.965 ± 0.435

1.668 ± 0.156

6.199 ± 0.275

6.376 ± 0.298

7.917 ± 0.284

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.209 ± 0.13

4.843 ± 0.255

3.9 ± 0.208

4.439 ± 0.328

5.761 ± 0.248

6.325 ± 0.272

5.062 ± 0.198

5.929 ± 0.247

1.407 ± 0.144

3.428 ± 0.213
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski