Amino acid dipepetide frequency for Mycobacterium phage SirPhilip

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
20.137AlaAla: 20.137 ± 1.38
1.157AlaCys: 1.157 ± 0.294
9.253AlaAsp: 9.253 ± 0.71
9.043AlaGlu: 9.043 ± 1.034
3.417AlaPhe: 3.417 ± 0.467
8.991AlaGly: 8.991 ± 0.806
2.787AlaHis: 2.787 ± 0.344
5.626AlaIle: 5.626 ± 0.66
4.154AlaLys: 4.154 ± 0.49
11.514AlaLeu: 11.514 ± 0.632
3.891AlaMet: 3.891 ± 0.502
3.049AlaAsn: 3.049 ± 0.513
7.045AlaPro: 7.045 ± 0.606
5.047AlaGln: 5.047 ± 0.593
8.149AlaArg: 8.149 ± 0.889
5.468AlaSer: 5.468 ± 0.579
7.571AlaThr: 7.571 ± 0.865
9.989AlaVal: 9.989 ± 0.716
2.208AlaTrp: 2.208 ± 0.278
3.155AlaTyr: 3.155 ± 0.48
0.0AlaXaa: 0.0 ± 0.0
Cys
1.104CysAla: 1.104 ± 0.263
0.105CysCys: 0.105 ± 0.074
0.631CysAsp: 0.631 ± 0.182
0.736CysGlu: 0.736 ± 0.23
0.21CysPhe: 0.21 ± 0.102
1.577CysGly: 1.577 ± 0.398
0.21CysHis: 0.21 ± 0.123
0.368CysIle: 0.368 ± 0.12
0.473CysLys: 0.473 ± 0.146
0.631CysLeu: 0.631 ± 0.212
0.105CysMet: 0.105 ± 0.07
0.158CysAsn: 0.158 ± 0.091
0.683CysPro: 0.683 ± 0.188
0.105CysGln: 0.105 ± 0.084
0.736CysArg: 0.736 ± 0.192
1.052CysSer: 1.052 ± 0.23
0.421CysThr: 0.421 ± 0.157
0.841CysVal: 0.841 ± 0.235
0.368CysTrp: 0.368 ± 0.145
0.158CysTyr: 0.158 ± 0.103
0.0CysXaa: 0.0 ± 0.0
Asp
8.044AspAla: 8.044 ± 0.772
0.578AspCys: 0.578 ± 0.179
5.258AspAsp: 5.258 ± 0.539
5.889AspGlu: 5.889 ± 0.729
1.63AspPhe: 1.63 ± 0.356
7.413AspGly: 7.413 ± 0.531
0.999AspHis: 0.999 ± 0.271
1.472AspIle: 1.472 ± 0.288
1.998AspLys: 1.998 ± 0.328
6.099AspLeu: 6.099 ± 0.615
1.42AspMet: 1.42 ± 0.306
1.314AspAsn: 1.314 ± 0.217
4.416AspPro: 4.416 ± 0.406
2.156AspGln: 2.156 ± 0.273
5.31AspArg: 5.31 ± 0.591
2.471AspSer: 2.471 ± 0.36
2.787AspThr: 2.787 ± 0.373
4.048AspVal: 4.048 ± 0.437
0.946AspTrp: 0.946 ± 0.215
1.42AspTyr: 1.42 ± 0.284
0.0AspXaa: 0.0 ± 0.0
Glu
8.517GluAla: 8.517 ± 0.981
0.841GluCys: 0.841 ± 0.212
3.049GluAsp: 3.049 ± 0.435
0.736GluGlu: 0.736 ± 0.202
1.735GluPhe: 1.735 ± 0.37
4.522GluGly: 4.522 ± 0.519
1.893GluHis: 1.893 ± 0.348
2.313GluIle: 2.313 ± 0.325
1.367GluLys: 1.367 ± 0.307
4.942GluLeu: 4.942 ± 0.522
1.472GluMet: 1.472 ± 0.356
1.42GluAsn: 1.42 ± 0.356
3.838GluPro: 3.838 ± 0.569
2.103GluGln: 2.103 ± 0.366
5.047GluArg: 5.047 ± 0.543
2.576GluSer: 2.576 ± 0.343
2.629GluThr: 2.629 ± 0.372
6.257GluVal: 6.257 ± 0.63
1.157GluTrp: 1.157 ± 0.203
1.314GluTyr: 1.314 ± 0.255
0.0GluXaa: 0.0 ± 0.0
Phe
2.839PheAla: 2.839 ± 0.513
0.263PheCys: 0.263 ± 0.121
2.839PheAsp: 2.839 ± 0.407
1.104PheGlu: 1.104 ± 0.191
0.526PhePhe: 0.526 ± 0.192
2.892PheGly: 2.892 ± 0.335
0.21PheHis: 0.21 ± 0.109
0.789PheIle: 0.789 ± 0.185
1.788PheLys: 1.788 ± 0.474
1.84PheLeu: 1.84 ± 0.329
0.421PheMet: 0.421 ± 0.142
1.052PheAsn: 1.052 ± 0.306
1.262PhePro: 1.262 ± 0.208
0.526PheGln: 0.526 ± 0.164
1.209PheArg: 1.209 ± 0.248
1.577PheSer: 1.577 ± 0.298
1.945PheThr: 1.945 ± 0.305
2.208PheVal: 2.208 ± 0.421
0.578PheTrp: 0.578 ± 0.146
0.421PheTyr: 0.421 ± 0.128
0.0PheXaa: 0.0 ± 0.0
Gly
10.988GlyAla: 10.988 ± 0.806
0.999GlyCys: 0.999 ± 0.275
5.258GlyAsp: 5.258 ± 0.493
5.468GlyGlu: 5.468 ± 0.555
2.313GlyPhe: 2.313 ± 0.488
10.2GlyGly: 10.2 ± 1.53
1.893GlyHis: 1.893 ± 0.331
2.787GlyIle: 2.787 ± 0.512
4.101GlyLys: 4.101 ± 0.41
7.045GlyLeu: 7.045 ± 0.779
1.577GlyMet: 1.577 ± 0.261
2.787GlyAsn: 2.787 ± 0.382
3.838GlyPro: 3.838 ± 0.55
2.156GlyGln: 2.156 ± 0.351
5.994GlyArg: 5.994 ± 0.687
5.1GlySer: 5.1 ± 0.674
5.31GlyThr: 5.31 ± 0.536
6.309GlyVal: 6.309 ± 0.578
2.419GlyTrp: 2.419 ± 0.393
2.261GlyTyr: 2.261 ± 0.307
0.0GlyXaa: 0.0 ± 0.0
His
2.261HisAla: 2.261 ± 0.448
0.315HisCys: 0.315 ± 0.116
1.262HisAsp: 1.262 ± 0.268
1.052HisGlu: 1.052 ± 0.233
0.631HisPhe: 0.631 ± 0.187
2.156HisGly: 2.156 ± 0.341
0.736HisHis: 0.736 ± 0.169
0.999HisIle: 0.999 ± 0.216
0.368HisLys: 0.368 ± 0.148
1.998HisLeu: 1.998 ± 0.256
0.421HisMet: 0.421 ± 0.127
0.631HisAsn: 0.631 ± 0.142
1.157HisPro: 1.157 ± 0.24
0.578HisGln: 0.578 ± 0.211
2.05HisArg: 2.05 ± 0.333
1.052HisSer: 1.052 ± 0.21
1.262HisThr: 1.262 ± 0.269
2.103HisVal: 2.103 ± 0.305
0.578HisTrp: 0.578 ± 0.163
0.683HisTyr: 0.683 ± 0.216
0.0HisXaa: 0.0 ± 0.0
Ile
5.31IleAla: 5.31 ± 0.615
0.315IleCys: 0.315 ± 0.136
3.785IleAsp: 3.785 ± 0.451
3.207IleGlu: 3.207 ± 0.462
0.894IlePhe: 0.894 ± 0.22
4.048IleGly: 4.048 ± 0.821
0.421IleHis: 0.421 ± 0.145
0.683IleIle: 0.683 ± 0.169
1.262IleLys: 1.262 ± 0.222
2.471IleLeu: 2.471 ± 0.3
0.421IleMet: 0.421 ± 0.128
1.42IleAsn: 1.42 ± 0.241
1.893IlePro: 1.893 ± 0.337
0.526IleGln: 0.526 ± 0.148
2.261IleArg: 2.261 ± 0.274
1.577IleSer: 1.577 ± 0.33
2.156IleThr: 2.156 ± 0.308
3.207IleVal: 3.207 ± 0.379
0.841IleTrp: 0.841 ± 0.208
0.421IleTyr: 0.421 ± 0.146
0.0IleXaa: 0.0 ± 0.0
Lys
4.522LysAla: 4.522 ± 0.581
0.368LysCys: 0.368 ± 0.144
1.262LysAsp: 1.262 ± 0.241
0.841LysGlu: 0.841 ± 0.203
0.789LysPhe: 0.789 ± 0.209
2.944LysGly: 2.944 ± 0.372
0.631LysHis: 0.631 ± 0.148
1.42LysIle: 1.42 ± 0.263
0.578LysLys: 0.578 ± 0.17
3.365LysLeu: 3.365 ± 0.321
0.683LysMet: 0.683 ± 0.215
0.736LysAsn: 0.736 ± 0.19
2.839LysPro: 2.839 ± 0.402
0.946LysGln: 0.946 ± 0.204
2.839LysArg: 2.839 ± 0.393
1.104LysSer: 1.104 ± 0.231
2.261LysThr: 2.261 ± 0.462
2.944LysVal: 2.944 ± 0.462
0.526LysTrp: 0.526 ± 0.139
0.946LysTyr: 0.946 ± 0.248
0.0LysXaa: 0.0 ± 0.0
Leu
12.355LeuAla: 12.355 ± 0.831
0.999LeuCys: 0.999 ± 0.261
7.413LeuAsp: 7.413 ± 0.656
2.419LeuGlu: 2.419 ± 0.378
2.103LeuPhe: 2.103 ± 0.369
6.887LeuGly: 6.887 ± 0.599
2.629LeuHis: 2.629 ± 0.38
3.575LeuIle: 3.575 ± 0.419
2.156LeuLys: 2.156 ± 0.308
6.257LeuLeu: 6.257 ± 0.662
2.05LeuMet: 2.05 ± 0.354
2.366LeuAsn: 2.366 ± 0.341
3.996LeuPro: 3.996 ± 0.522
2.313LeuGln: 2.313 ± 0.367
5.941LeuArg: 5.941 ± 0.676
5.994LeuSer: 5.994 ± 0.592
5.258LeuThr: 5.258 ± 0.409
6.362LeuVal: 6.362 ± 0.514
1.735LeuTrp: 1.735 ± 0.346
1.525LeuTyr: 1.525 ± 0.365
0.0LeuXaa: 0.0 ± 0.0
Met
2.471MetAla: 2.471 ± 0.32
0.21MetCys: 0.21 ± 0.094
1.314MetAsp: 1.314 ± 0.251
0.736MetGlu: 0.736 ± 0.174
0.736MetPhe: 0.736 ± 0.198
1.262MetGly: 1.262 ± 0.283
0.683MetHis: 0.683 ± 0.152
0.999MetIle: 0.999 ± 0.207
0.21MetLys: 0.21 ± 0.098
1.577MetLeu: 1.577 ± 0.291
0.631MetMet: 0.631 ± 0.159
0.683MetAsn: 0.683 ± 0.202
1.525MetPro: 1.525 ± 0.263
0.841MetGln: 0.841 ± 0.207
2.05MetArg: 2.05 ± 0.323
2.156MetSer: 2.156 ± 0.303
1.472MetThr: 1.472 ± 0.27
1.367MetVal: 1.367 ± 0.316
0.473MetTrp: 0.473 ± 0.133
0.421MetTyr: 0.421 ± 0.181
0.0MetXaa: 0.0 ± 0.0
Asn
3.47AsnAla: 3.47 ± 0.441
0.263AsnCys: 0.263 ± 0.113
1.788AsnAsp: 1.788 ± 0.296
1.262AsnGlu: 1.262 ± 0.236
0.526AsnPhe: 0.526 ± 0.162
4.048AsnGly: 4.048 ± 0.579
0.421AsnHis: 0.421 ± 0.148
0.631AsnIle: 0.631 ± 0.211
0.894AsnLys: 0.894 ± 0.211
2.261AsnLeu: 2.261 ± 0.362
0.263AsnMet: 0.263 ± 0.106
0.526AsnAsn: 0.526 ± 0.178
2.313AsnPro: 2.313 ± 0.359
0.526AsnGln: 0.526 ± 0.126
2.103AsnArg: 2.103 ± 0.354
0.631AsnSer: 0.631 ± 0.177
1.262AsnThr: 1.262 ± 0.231
2.208AsnVal: 2.208 ± 0.339
0.421AsnTrp: 0.421 ± 0.14
0.578AsnTyr: 0.578 ± 0.15
0.0AsnXaa: 0.0 ± 0.0
Pro
8.517ProAla: 8.517 ± 0.602
0.421ProCys: 0.421 ± 0.175
3.102ProAsp: 3.102 ± 0.36
4.679ProGlu: 4.679 ± 0.609
1.788ProPhe: 1.788 ± 0.24
5.731ProGly: 5.731 ± 0.51
1.262ProHis: 1.262 ± 0.283
1.84ProIle: 1.84 ± 0.285
1.84ProLys: 1.84 ± 0.28
3.996ProLeu: 3.996 ± 0.474
0.841ProMet: 0.841 ± 0.215
1.577ProAsn: 1.577 ± 0.356
3.575ProPro: 3.575 ± 0.502
1.157ProGln: 1.157 ± 0.304
2.734ProArg: 2.734 ± 0.41
3.26ProSer: 3.26 ± 0.484
3.365ProThr: 3.365 ± 0.518
5.047ProVal: 5.047 ± 0.534
0.841ProTrp: 0.841 ± 0.168
1.63ProTyr: 1.63 ± 0.264
0.0ProXaa: 0.0 ± 0.0
Gln
5.521GlnAla: 5.521 ± 0.543
0.053GlnCys: 0.053 ± 0.054
0.789GlnAsp: 0.789 ± 0.204
1.052GlnGlu: 1.052 ± 0.227
0.683GlnPhe: 0.683 ± 0.18
2.156GlnGly: 2.156 ± 0.334
0.841GlnHis: 0.841 ± 0.193
1.63GlnIle: 1.63 ± 0.322
0.789GlnLys: 0.789 ± 0.201
2.734GlnLeu: 2.734 ± 0.446
0.841GlnMet: 0.841 ± 0.222
0.473GlnAsn: 0.473 ± 0.156
1.63GlnPro: 1.63 ± 0.298
0.841GlnGln: 0.841 ± 0.25
2.681GlnArg: 2.681 ± 0.353
1.63GlnSer: 1.63 ± 0.271
1.788GlnThr: 1.788 ± 0.311
2.261GlnVal: 2.261 ± 0.287
0.841GlnTrp: 0.841 ± 0.215
0.789GlnTyr: 0.789 ± 0.203
0.0GlnXaa: 0.0 ± 0.0
Arg
6.362ArgAla: 6.362 ± 0.844
1.052ArgCys: 1.052 ± 0.263
4.206ArgAsp: 4.206 ± 0.524
5.152ArgGlu: 5.152 ± 0.596
2.103ArgPhe: 2.103 ± 0.323
4.364ArgGly: 4.364 ± 0.464
1.577ArgHis: 1.577 ± 0.345
2.419ArgIle: 2.419 ± 0.351
3.049ArgLys: 3.049 ± 0.456
6.204ArgLeu: 6.204 ± 0.643
2.103ArgMet: 2.103 ± 0.322
2.419ArgAsn: 2.419 ± 0.35
3.575ArgPro: 3.575 ± 0.449
2.997ArgGln: 2.997 ± 0.324
5.889ArgArg: 5.889 ± 0.685
3.838ArgSer: 3.838 ± 0.435
3.68ArgThr: 3.68 ± 0.503
4.942ArgVal: 4.942 ± 0.521
1.945ArgTrp: 1.945 ± 0.381
2.313ArgTyr: 2.313 ± 0.38
0.0ArgXaa: 0.0 ± 0.0
Ser
6.362SerAla: 6.362 ± 0.695
0.263SerCys: 0.263 ± 0.129
3.155SerAsp: 3.155 ± 0.323
2.839SerGlu: 2.839 ± 0.47
1.314SerPhe: 1.314 ± 0.266
4.206SerGly: 4.206 ± 0.517
0.789SerHis: 0.789 ± 0.179
2.524SerIle: 2.524 ± 0.416
1.314SerLys: 1.314 ± 0.252
4.311SerLeu: 4.311 ± 0.405
1.42SerMet: 1.42 ± 0.294
1.525SerAsn: 1.525 ± 0.393
3.891SerPro: 3.891 ± 0.444
1.735SerGln: 1.735 ± 0.236
2.839SerArg: 2.839 ± 0.408
3.26SerSer: 3.26 ± 0.36
4.311SerThr: 4.311 ± 0.399
3.943SerVal: 3.943 ± 0.43
0.999SerTrp: 0.999 ± 0.205
1.314SerTyr: 1.314 ± 0.218
0.0SerXaa: 0.0 ± 0.0
Thr
6.625ThrAla: 6.625 ± 0.673
0.999ThrCys: 0.999 ± 0.213
3.523ThrAsp: 3.523 ± 0.478
3.417ThrGlu: 3.417 ± 0.316
1.945ThrPhe: 1.945 ± 0.311
5.783ThrGly: 5.783 ± 0.576
1.42ThrHis: 1.42 ± 0.254
2.944ThrIle: 2.944 ± 0.47
2.576ThrLys: 2.576 ± 0.434
4.259ThrLeu: 4.259 ± 0.582
0.999ThrMet: 0.999 ± 0.214
1.42ThrAsn: 1.42 ± 0.332
3.26ThrPro: 3.26 ± 0.469
1.262ThrGln: 1.262 ± 0.32
3.155ThrArg: 3.155 ± 0.536
3.523ThrSer: 3.523 ± 0.515
4.679ThrThr: 4.679 ± 0.624
5.521ThrVal: 5.521 ± 0.435
1.314ThrTrp: 1.314 ± 0.243
1.788ThrTyr: 1.788 ± 0.466
0.0ThrXaa: 0.0 ± 0.0
Val
10.936ValAla: 10.936 ± 0.924
0.894ValCys: 0.894 ± 0.181
5.521ValAsp: 5.521 ± 0.56
6.099ValGlu: 6.099 ± 0.68
1.682ValPhe: 1.682 ± 0.363
6.099ValGly: 6.099 ± 0.669
1.945ValHis: 1.945 ± 0.355
2.997ValIle: 2.997 ± 0.433
2.787ValLys: 2.787 ± 0.385
6.993ValLeu: 6.993 ± 0.542
1.525ValMet: 1.525 ± 0.298
1.525ValAsn: 1.525 ± 0.275
4.627ValPro: 4.627 ± 0.581
2.261ValGln: 2.261 ± 0.305
4.732ValArg: 4.732 ± 0.419
3.838ValSer: 3.838 ± 0.389
5.415ValThr: 5.415 ± 0.56
6.94ValVal: 6.94 ± 0.851
1.682ValTrp: 1.682 ± 0.298
1.682ValTyr: 1.682 ± 0.244
0.0ValXaa: 0.0 ± 0.0
Trp
2.366TrpAla: 2.366 ± 0.403
0.315TrpCys: 0.315 ± 0.124
0.736TrpAsp: 0.736 ± 0.218
0.789TrpGlu: 0.789 ± 0.246
0.578TrpPhe: 0.578 ± 0.155
1.157TrpGly: 1.157 ± 0.234
0.789TrpHis: 0.789 ± 0.207
0.631TrpIle: 0.631 ± 0.148
0.263TrpLys: 0.263 ± 0.119
3.049TrpLeu: 3.049 ± 0.37
0.368TrpMet: 0.368 ± 0.135
0.683TrpAsn: 0.683 ± 0.179
0.894TrpPro: 0.894 ± 0.195
1.262TrpGln: 1.262 ± 0.245
2.103TrpArg: 2.103 ± 0.345
1.052TrpSer: 1.052 ± 0.255
1.262TrpThr: 1.262 ± 0.227
1.472TrpVal: 1.472 ± 0.254
0.368TrpTrp: 0.368 ± 0.126
0.421TrpTyr: 0.421 ± 0.136
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.944TyrAla: 2.944 ± 0.385
0.263TyrCys: 0.263 ± 0.133
1.525TyrAsp: 1.525 ± 0.301
1.209TyrGlu: 1.209 ± 0.287
0.683TyrPhe: 0.683 ± 0.167
2.366TyrGly: 2.366 ± 0.378
0.158TyrHis: 0.158 ± 0.088
0.473TyrIle: 0.473 ± 0.14
0.578TyrLys: 0.578 ± 0.174
2.787TyrLeu: 2.787 ± 0.525
0.315TyrMet: 0.315 ± 0.124
0.631TyrAsn: 0.631 ± 0.171
0.999TyrPro: 0.999 ± 0.295
0.683TyrGln: 0.683 ± 0.203
2.366TyrArg: 2.366 ± 0.351
1.157TyrSer: 1.157 ± 0.252
1.577TyrThr: 1.577 ± 0.28
2.208TyrVal: 2.208 ± 0.346
0.315TyrTrp: 0.315 ± 0.12
0.578TyrTyr: 0.578 ± 0.159
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 97 proteins (19021 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski