Amino acid dipepetide frequency for Pseudomonas phage UNO-SLW3

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
11.428AlaAla: 11.428 ± 1.213
0.658AlaCys: 0.658 ± 0.307
6.166AlaAsp: 6.166 ± 0.891
6.084AlaGlu: 6.084 ± 0.641
3.453AlaPhe: 3.453 ± 0.599
7.811AlaGly: 7.811 ± 0.792
2.22AlaHis: 2.22 ± 0.468
3.535AlaIle: 3.535 ± 0.657
6.331AlaLys: 6.331 ± 0.513
8.797AlaLeu: 8.797 ± 1.177
3.535AlaMet: 3.535 ± 0.601
4.111AlaAsn: 4.111 ± 0.661
3.453AlaPro: 3.453 ± 0.602
5.755AlaGln: 5.755 ± 1.056
6.495AlaArg: 6.495 ± 0.757
7.071AlaSer: 7.071 ± 0.957
4.933AlaThr: 4.933 ± 1.255
6.495AlaVal: 6.495 ± 0.627
1.315AlaTrp: 1.315 ± 0.404
3.206AlaTyr: 3.206 ± 0.511
0.0AlaXaa: 0.0 ± 0.0
Cys
1.069CysAla: 1.069 ± 0.326
0.0CysCys: 0.0 ± 0.0
0.658CysAsp: 0.658 ± 0.333
0.493CysGlu: 0.493 ± 0.164
0.411CysPhe: 0.411 ± 0.218
0.822CysGly: 0.822 ± 0.312
0.164CysHis: 0.164 ± 0.105
0.247CysIle: 0.247 ± 0.121
0.493CysLys: 0.493 ± 0.195
1.069CysLeu: 1.069 ± 0.334
0.247CysMet: 0.247 ± 0.136
0.082CysAsn: 0.082 ± 0.079
0.164CysPro: 0.164 ± 0.118
0.411CysGln: 0.411 ± 0.221
0.576CysArg: 0.576 ± 0.2
0.329CysSer: 0.329 ± 0.134
0.329CysThr: 0.329 ± 0.178
0.329CysVal: 0.329 ± 0.182
0.164CysTrp: 0.164 ± 0.118
0.247CysTyr: 0.247 ± 0.136
0.0CysXaa: 0.0 ± 0.0
Asp
6.495AspAla: 6.495 ± 0.676
0.411AspCys: 0.411 ± 0.186
4.686AspAsp: 4.686 ± 0.703
3.535AspGlu: 3.535 ± 0.508
3.124AspPhe: 3.124 ± 0.505
6.824AspGly: 6.824 ± 0.678
1.48AspHis: 1.48 ± 0.429
3.453AspIle: 3.453 ± 0.437
3.206AspLys: 3.206 ± 0.558
4.111AspLeu: 4.111 ± 0.622
1.891AspMet: 1.891 ± 0.461
1.48AspAsn: 1.48 ± 0.305
3.124AspPro: 3.124 ± 0.656
2.384AspGln: 2.384 ± 0.368
3.946AspArg: 3.946 ± 0.621
3.042AspSer: 3.042 ± 0.77
3.124AspThr: 3.124 ± 0.487
3.782AspVal: 3.782 ± 0.638
1.233AspTrp: 1.233 ± 0.41
1.727AspTyr: 1.727 ± 0.297
0.0AspXaa: 0.0 ± 0.0
Glu
8.551GluAla: 8.551 ± 0.767
0.822GluCys: 0.822 ± 0.374
4.111GluAsp: 4.111 ± 0.404
5.097GluGlu: 5.097 ± 0.853
2.466GluPhe: 2.466 ± 0.429
5.18GluGly: 5.18 ± 0.685
1.562GluHis: 1.562 ± 0.362
2.795GluIle: 2.795 ± 0.377
3.371GluLys: 3.371 ± 0.461
5.755GluLeu: 5.755 ± 0.806
1.973GluMet: 1.973 ± 0.476
2.795GluAsn: 2.795 ± 0.376
2.138GluPro: 2.138 ± 0.476
2.302GluGln: 2.302 ± 0.337
3.782GluArg: 3.782 ± 0.561
3.535GluSer: 3.535 ± 0.451
4.357GluThr: 4.357 ± 0.587
4.193GluVal: 4.193 ± 0.696
1.398GluTrp: 1.398 ± 0.42
2.138GluTyr: 2.138 ± 0.357
0.0GluXaa: 0.0 ± 0.0
Phe
2.055PheAla: 2.055 ± 0.545
0.493PheCys: 0.493 ± 0.273
3.042PheAsp: 3.042 ± 0.659
2.138PheGlu: 2.138 ± 0.382
1.398PhePhe: 1.398 ± 0.264
2.96PheGly: 2.96 ± 0.496
0.822PheHis: 0.822 ± 0.231
1.315PheIle: 1.315 ± 0.372
2.138PheLys: 2.138 ± 0.469
3.618PheLeu: 3.618 ± 0.485
1.233PheMet: 1.233 ± 0.275
1.891PheAsn: 1.891 ± 0.416
1.562PhePro: 1.562 ± 0.371
0.74PheGln: 0.74 ± 0.214
2.384PheArg: 2.384 ± 0.387
1.727PheSer: 1.727 ± 0.303
2.549PheThr: 2.549 ± 0.405
2.138PheVal: 2.138 ± 0.379
0.576PheTrp: 0.576 ± 0.203
0.822PheTyr: 0.822 ± 0.386
0.0PheXaa: 0.0 ± 0.0
Gly
7.399GlyAla: 7.399 ± 1.178
0.904GlyCys: 0.904 ± 0.284
4.933GlyAsp: 4.933 ± 0.574
5.426GlyGlu: 5.426 ± 0.602
3.782GlyPhe: 3.782 ± 0.645
7.153GlyGly: 7.153 ± 0.916
1.644GlyHis: 1.644 ± 0.316
4.522GlyIle: 4.522 ± 0.728
4.522GlyLys: 4.522 ± 0.886
6.906GlyLeu: 6.906 ± 0.826
2.055GlyMet: 2.055 ± 0.445
3.042GlyAsn: 3.042 ± 0.457
2.549GlyPro: 2.549 ± 0.483
2.549GlyGln: 2.549 ± 0.483
5.509GlyArg: 5.509 ± 0.691
5.426GlySer: 5.426 ± 0.525
4.111GlyThr: 4.111 ± 0.47
5.426GlyVal: 5.426 ± 0.654
1.48GlyTrp: 1.48 ± 0.304
3.124GlyTyr: 3.124 ± 0.595
0.0GlyXaa: 0.0 ± 0.0
His
1.562HisAla: 1.562 ± 0.367
0.411HisCys: 0.411 ± 0.173
1.233HisAsp: 1.233 ± 0.394
1.727HisGlu: 1.727 ± 0.378
0.904HisPhe: 0.904 ± 0.277
1.891HisGly: 1.891 ± 0.412
0.493HisHis: 0.493 ± 0.227
0.904HisIle: 0.904 ± 0.252
1.315HisLys: 1.315 ± 0.296
2.302HisLeu: 2.302 ± 0.352
0.493HisMet: 0.493 ± 0.171
0.987HisAsn: 0.987 ± 0.372
0.74HisPro: 0.74 ± 0.214
0.74HisGln: 0.74 ± 0.214
1.398HisArg: 1.398 ± 0.384
1.069HisSer: 1.069 ± 0.306
1.151HisThr: 1.151 ± 0.274
1.644HisVal: 1.644 ± 0.426
0.658HisTrp: 0.658 ± 0.199
0.904HisTyr: 0.904 ± 0.271
0.0HisXaa: 0.0 ± 0.0
Ile
3.453IleAla: 3.453 ± 0.5
0.576IleCys: 0.576 ± 0.229
2.96IleAsp: 2.96 ± 0.558
3.618IleGlu: 3.618 ± 0.593
0.904IlePhe: 0.904 ± 0.264
3.206IleGly: 3.206 ± 0.517
0.904IleHis: 0.904 ± 0.376
2.302IleIle: 2.302 ± 0.379
2.878IleLys: 2.878 ± 0.611
3.864IleLeu: 3.864 ± 0.505
1.398IleMet: 1.398 ± 0.36
1.315IleAsn: 1.315 ± 0.352
2.466IlePro: 2.466 ± 0.387
2.138IleGln: 2.138 ± 0.478
3.124IleArg: 3.124 ± 0.337
1.891IleSer: 1.891 ± 0.347
2.795IleThr: 2.795 ± 0.392
3.206IleVal: 3.206 ± 0.419
0.329IleTrp: 0.329 ± 0.178
1.809IleTyr: 1.809 ± 0.56
0.0IleXaa: 0.0 ± 0.0
Lys
8.139LysAla: 8.139 ± 0.965
0.493LysCys: 0.493 ± 0.198
4.193LysAsp: 4.193 ± 0.591
4.029LysGlu: 4.029 ± 0.551
2.549LysPhe: 2.549 ± 0.44
4.933LysGly: 4.933 ± 0.69
1.727LysHis: 1.727 ± 0.502
2.384LysIle: 2.384 ± 0.383
3.206LysLys: 3.206 ± 0.855
5.015LysLeu: 5.015 ± 0.612
1.727LysMet: 1.727 ± 0.415
1.973LysAsn: 1.973 ± 0.542
2.549LysPro: 2.549 ± 0.497
2.22LysGln: 2.22 ± 0.442
3.371LysArg: 3.371 ± 0.803
2.549LysSer: 2.549 ± 0.369
3.535LysThr: 3.535 ± 0.565
3.618LysVal: 3.618 ± 0.533
0.576LysTrp: 0.576 ± 0.185
1.151LysTyr: 1.151 ± 0.225
0.0LysXaa: 0.0 ± 0.0
Leu
8.386LeuAla: 8.386 ± 1.001
0.164LeuCys: 0.164 ± 0.122
5.015LeuAsp: 5.015 ± 0.716
6.331LeuGlu: 6.331 ± 0.851
2.384LeuPhe: 2.384 ± 0.576
5.262LeuGly: 5.262 ± 0.621
2.055LeuHis: 2.055 ± 0.423
3.618LeuIle: 3.618 ± 0.596
6.988LeuLys: 6.988 ± 0.569
5.92LeuLeu: 5.92 ± 0.899
2.795LeuMet: 2.795 ± 0.467
3.7LeuAsn: 3.7 ± 0.466
2.795LeuPro: 2.795 ± 0.477
4.029LeuGln: 4.029 ± 0.49
4.604LeuArg: 4.604 ± 0.536
5.015LeuSer: 5.015 ± 0.73
5.18LeuThr: 5.18 ± 0.817
5.92LeuVal: 5.92 ± 0.614
1.069LeuTrp: 1.069 ± 0.28
1.891LeuTyr: 1.891 ± 0.364
0.0LeuXaa: 0.0 ± 0.0
Met
3.946MetAla: 3.946 ± 0.465
0.164MetCys: 0.164 ± 0.142
1.644MetAsp: 1.644 ± 0.415
2.549MetGlu: 2.549 ± 0.574
0.329MetPhe: 0.329 ± 0.183
2.549MetGly: 2.549 ± 0.434
0.576MetHis: 0.576 ± 0.205
1.398MetIle: 1.398 ± 0.27
1.151MetLys: 1.151 ± 0.304
2.549MetLeu: 2.549 ± 0.492
0.658MetMet: 0.658 ± 0.232
1.233MetAsn: 1.233 ± 0.357
1.398MetPro: 1.398 ± 0.281
0.822MetGln: 0.822 ± 0.253
1.315MetArg: 1.315 ± 0.267
2.22MetSer: 2.22 ± 0.402
2.138MetThr: 2.138 ± 0.386
2.138MetVal: 2.138 ± 0.39
0.329MetTrp: 0.329 ± 0.147
0.411MetTyr: 0.411 ± 0.163
0.0MetXaa: 0.0 ± 0.0
Asn
3.618AsnAla: 3.618 ± 0.528
0.164AsnCys: 0.164 ± 0.113
1.973AsnAsp: 1.973 ± 0.386
2.302AsnGlu: 2.302 ± 0.458
2.22AsnPhe: 2.22 ± 0.356
3.7AsnGly: 3.7 ± 0.504
0.904AsnHis: 0.904 ± 0.337
1.644AsnIle: 1.644 ± 0.426
1.644AsnLys: 1.644 ± 0.307
4.193AsnLeu: 4.193 ± 0.471
1.151AsnMet: 1.151 ± 0.322
1.069AsnAsn: 1.069 ± 0.359
2.795AsnPro: 2.795 ± 0.436
0.987AsnGln: 0.987 ± 0.211
2.466AsnArg: 2.466 ± 0.431
0.74AsnSer: 0.74 ± 0.251
2.466AsnThr: 2.466 ± 0.465
2.878AsnVal: 2.878 ± 0.469
0.247AsnTrp: 0.247 ± 0.16
1.48AsnTyr: 1.48 ± 0.385
0.0AsnXaa: 0.0 ± 0.0
Pro
2.878ProAla: 2.878 ± 0.484
0.247ProCys: 0.247 ± 0.145
3.864ProAsp: 3.864 ± 0.498
3.7ProGlu: 3.7 ± 0.603
1.48ProPhe: 1.48 ± 0.321
2.549ProGly: 2.549 ± 0.383
0.904ProHis: 0.904 ± 0.258
1.398ProIle: 1.398 ± 0.401
2.631ProLys: 2.631 ± 0.477
2.22ProLeu: 2.22 ± 0.468
0.904ProMet: 0.904 ± 0.292
2.302ProAsn: 2.302 ± 0.383
1.398ProPro: 1.398 ± 0.357
1.398ProGln: 1.398 ± 0.261
2.138ProArg: 2.138 ± 0.383
2.22ProSer: 2.22 ± 0.519
2.22ProThr: 2.22 ± 0.409
3.206ProVal: 3.206 ± 0.618
0.576ProTrp: 0.576 ± 0.212
0.904ProTyr: 0.904 ± 0.315
0.0ProXaa: 0.0 ± 0.0
Gln
5.755GlnAla: 5.755 ± 1.035
0.082GlnCys: 0.082 ± 0.081
2.384GlnAsp: 2.384 ± 0.557
2.22GlnGlu: 2.22 ± 0.427
2.138GlnPhe: 2.138 ± 0.533
3.535GlnGly: 3.535 ± 0.555
0.411GlnHis: 0.411 ± 0.19
2.22GlnIle: 2.22 ± 0.344
1.973GlnLys: 1.973 ± 0.579
3.946GlnLeu: 3.946 ± 0.451
1.562GlnMet: 1.562 ± 0.44
1.069GlnAsn: 1.069 ± 0.297
0.904GlnPro: 0.904 ± 0.238
1.727GlnGln: 1.727 ± 0.351
2.302GlnArg: 2.302 ± 0.491
1.809GlnSer: 1.809 ± 0.368
1.562GlnThr: 1.562 ± 0.392
2.795GlnVal: 2.795 ± 0.397
0.658GlnTrp: 0.658 ± 0.239
1.562GlnTyr: 1.562 ± 0.478
0.0GlnXaa: 0.0 ± 0.0
Arg
6.166ArgAla: 6.166 ± 0.718
0.576ArgCys: 0.576 ± 0.306
3.453ArgAsp: 3.453 ± 0.649
4.275ArgGlu: 4.275 ± 0.566
1.644ArgPhe: 1.644 ± 0.32
5.262ArgGly: 5.262 ± 0.524
1.48ArgHis: 1.48 ± 0.373
2.878ArgIle: 2.878 ± 0.443
3.206ArgLys: 3.206 ± 0.581
4.851ArgLeu: 4.851 ± 0.577
1.727ArgMet: 1.727 ± 0.451
3.124ArgAsn: 3.124 ± 0.603
2.466ArgPro: 2.466 ± 0.453
3.371ArgGln: 3.371 ± 0.504
3.371ArgArg: 3.371 ± 0.461
3.289ArgSer: 3.289 ± 0.479
2.466ArgThr: 2.466 ± 0.384
3.535ArgVal: 3.535 ± 0.542
0.658ArgTrp: 0.658 ± 0.229
1.891ArgTyr: 1.891 ± 0.32
0.0ArgXaa: 0.0 ± 0.0
Ser
5.262SerAla: 5.262 ± 0.839
0.576SerCys: 0.576 ± 0.199
3.453SerAsp: 3.453 ± 0.419
2.795SerGlu: 2.795 ± 0.407
2.22SerPhe: 2.22 ± 0.357
5.262SerGly: 5.262 ± 0.653
1.315SerHis: 1.315 ± 0.353
2.549SerIle: 2.549 ± 0.425
3.206SerLys: 3.206 ± 0.516
4.44SerLeu: 4.44 ± 0.664
1.562SerMet: 1.562 ± 0.417
2.138SerAsn: 2.138 ± 0.54
1.973SerPro: 1.973 ± 0.382
1.809SerGln: 1.809 ± 0.408
3.124SerArg: 3.124 ± 0.462
2.713SerSer: 2.713 ± 0.425
2.466SerThr: 2.466 ± 0.424
3.7SerVal: 3.7 ± 0.736
0.904SerTrp: 0.904 ± 0.232
1.973SerTyr: 1.973 ± 0.357
0.0SerXaa: 0.0 ± 0.0
Thr
5.097ThrAla: 5.097 ± 0.61
0.493ThrCys: 0.493 ± 0.185
3.042ThrAsp: 3.042 ± 0.487
4.851ThrGlu: 4.851 ± 0.608
1.562ThrPhe: 1.562 ± 0.475
5.18ThrGly: 5.18 ± 0.913
1.233ThrHis: 1.233 ± 0.254
3.289ThrIle: 3.289 ± 0.393
4.357ThrLys: 4.357 ± 0.587
5.426ThrLeu: 5.426 ± 0.69
1.151ThrMet: 1.151 ± 0.262
2.055ThrAsn: 2.055 ± 0.487
3.042ThrPro: 3.042 ± 0.555
2.466ThrGln: 2.466 ± 0.482
2.466ThrArg: 2.466 ± 0.645
2.631ThrSer: 2.631 ± 0.569
3.535ThrThr: 3.535 ± 0.652
3.7ThrVal: 3.7 ± 0.651
0.411ThrTrp: 0.411 ± 0.194
1.233ThrTyr: 1.233 ± 0.314
0.0ThrXaa: 0.0 ± 0.0
Val
6.906ValAla: 6.906 ± 0.834
0.411ValCys: 0.411 ± 0.175
3.453ValAsp: 3.453 ± 0.503
4.522ValGlu: 4.522 ± 0.582
1.562ValPhe: 1.562 ± 0.369
4.851ValGly: 4.851 ± 0.597
1.48ValHis: 1.48 ± 0.374
2.96ValIle: 2.96 ± 0.549
4.522ValLys: 4.522 ± 0.848
4.604ValLeu: 4.604 ± 0.626
1.48ValMet: 1.48 ± 0.327
2.713ValAsn: 2.713 ± 0.56
2.466ValPro: 2.466 ± 0.393
2.878ValGln: 2.878 ± 0.417
4.686ValArg: 4.686 ± 0.612
3.946ValSer: 3.946 ± 0.456
5.344ValThr: 5.344 ± 0.654
4.44ValVal: 4.44 ± 0.779
1.233ValTrp: 1.233 ± 0.367
1.973ValTyr: 1.973 ± 0.443
0.082ValXaa: 0.082 ± 0.085
Trp
1.562TrpAla: 1.562 ± 0.394
0.164TrpCys: 0.164 ± 0.129
0.822TrpAsp: 0.822 ± 0.217
0.576TrpGlu: 0.576 ± 0.213
0.493TrpPhe: 0.493 ± 0.207
0.987TrpGly: 0.987 ± 0.248
0.658TrpHis: 0.658 ± 0.258
0.576TrpIle: 0.576 ± 0.253
1.315TrpLys: 1.315 ± 0.378
1.644TrpLeu: 1.644 ± 0.402
0.493TrpMet: 0.493 ± 0.201
0.576TrpAsn: 0.576 ± 0.252
0.411TrpPro: 0.411 ± 0.218
0.576TrpGln: 0.576 ± 0.228
0.493TrpArg: 0.493 ± 0.177
1.069TrpSer: 1.069 ± 0.381
0.658TrpThr: 0.658 ± 0.296
1.315TrpVal: 1.315 ± 0.293
0.082TrpTrp: 0.082 ± 0.099
0.164TrpTyr: 0.164 ± 0.101
0.0TrpXaa: 0.0 ± 0.0
Tyr
3.124TyrAla: 3.124 ± 0.612
0.493TyrCys: 0.493 ± 0.239
1.973TyrAsp: 1.973 ± 0.423
2.138TyrGlu: 2.138 ± 0.394
0.576TyrPhe: 0.576 ± 0.223
2.549TyrGly: 2.549 ± 0.371
0.493TyrHis: 0.493 ± 0.241
1.069TyrIle: 1.069 ± 0.242
1.48TyrLys: 1.48 ± 0.347
1.891TyrLeu: 1.891 ± 0.379
1.398TyrMet: 1.398 ± 0.339
0.987TyrAsn: 0.987 ± 0.29
0.74TyrPro: 0.74 ± 0.228
1.315TyrGln: 1.315 ± 0.395
2.138TyrArg: 2.138 ± 0.327
1.151TyrSer: 1.151 ± 0.286
2.302TyrThr: 2.302 ± 0.527
2.055TyrVal: 2.055 ± 0.476
0.74TyrTrp: 0.74 ± 0.231
0.329TyrTyr: 0.329 ± 0.176
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.082XaaIle: 0.082 ± 0.085
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.247XaaXaa: 0.247 ± 0.254
Statistics based on 48 proteins (12164 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski