Puma lentivirus 14

Taxonomy: Viruses; Riboviria; Pararnavirae; Artverviricota; Revtraviricetes; Ortervirales; Retroviridae; Orthoretrovirinae; Lentivirus; Puma lentivirus

Average proteome isoelectric point is 7.17

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|Q84811|Q84811_9RETR Env polyprotein OS=Puma lentivirus 14 OX=32615 GN=env PE=4 SV=1
MM1 pKa = 7.19AQANPMDD8 pKa = 4.41NAWIGPEE15 pKa = 3.97EE16 pKa = 4.98GEE18 pKa = 4.08MLLDD22 pKa = 3.84FEE24 pKa = 5.12VVMMLHH30 pKa = 6.73EE31 pKa = 4.72GSNYY35 pKa = 9.82PGINPFRR42 pKa = 11.84DD43 pKa = 3.47PTLTPEE49 pKa = 4.72KK50 pKa = 9.89KK51 pKa = 9.7QQTAALLQPLLVRR64 pKa = 11.84IKK66 pKa = 10.87QNLLQQGKK74 pKa = 8.61VKK76 pKa = 10.56NWGKK80 pKa = 10.3QDD82 pKa = 3.11WDD84 pKa = 2.91KK85 pKa = 10.79MRR87 pKa = 11.84RR88 pKa = 11.84YY89 pKa = 9.73KK90 pKa = 10.71AGEE93 pKa = 3.83RR94 pKa = 11.84AWIKK98 pKa = 9.68FVKK101 pKa = 10.19IFGGIHH107 pKa = 5.85NMPKK111 pKa = 10.43EE112 pKa = 3.99EE113 pKa = 4.43DD114 pKa = 3.44LGTFSGDD121 pKa = 2.43IDD123 pKa = 4.4MITPHH128 pKa = 7.02SYY130 pKa = 10.83RR131 pKa = 11.84KK132 pKa = 8.29VTYY135 pKa = 8.49NWKK138 pKa = 8.86TSLCFFLLIGSAMLAYY154 pKa = 10.56SLIFTIIIKK163 pKa = 10.01QALAQAVTLALDD175 pKa = 4.15PPWVIPLKK183 pKa = 10.47YY184 pKa = 10.15RR185 pKa = 11.84EE186 pKa = 5.18DD187 pKa = 3.6INFQCIGNHH196 pKa = 5.85PEE198 pKa = 3.93CNLPKK203 pKa = 10.54EE204 pKa = 4.28VGDD207 pKa = 3.47WKK209 pKa = 11.37QNFTWVYY216 pKa = 5.99QTPINEE222 pKa = 4.54TIGLEE227 pKa = 4.03IYY229 pKa = 10.11AQEE232 pKa = 3.98IAAKK236 pKa = 9.97LWQQVFLQCKK246 pKa = 9.58KK247 pKa = 10.73GPRR250 pKa = 11.84DD251 pKa = 3.41LDD253 pKa = 3.56TAIKK257 pKa = 10.35YY258 pKa = 7.42WICFYY263 pKa = 10.58DD264 pKa = 3.57TAIKK268 pKa = 10.43YY269 pKa = 10.24LLGYY273 pKa = 8.01EE274 pKa = 4.92HH275 pKa = 7.21IQLCPLGGYY284 pKa = 9.47LVYY287 pKa = 11.01DD288 pKa = 3.69NTTKK292 pKa = 10.34EE293 pKa = 4.09ISMCTPPLSLRR304 pKa = 11.84LLNFTLSQEE313 pKa = 3.95KK314 pKa = 9.31WEE316 pKa = 4.3QEE318 pKa = 4.0PFTDD322 pKa = 5.04IVWFGNKK329 pKa = 9.38ALNTTVNNITQVQINVTMVCNVIVPEE355 pKa = 3.96QVGKK359 pKa = 10.77KK360 pKa = 9.57KK361 pKa = 10.71GRR363 pKa = 11.84FQFYY367 pKa = 11.08NEE369 pKa = 4.38FLGPWGGGRR378 pKa = 11.84FQEE381 pKa = 4.86IIVRR385 pKa = 11.84YY386 pKa = 8.38QDD388 pKa = 2.98WVNISDD394 pKa = 5.54PILDD398 pKa = 4.64LNCSGIPGVDD408 pKa = 4.1FNHH411 pKa = 6.73TEE413 pKa = 3.82GNYY416 pKa = 9.13TCIHH420 pKa = 6.26NKK422 pKa = 9.01TYY424 pKa = 10.49QEE426 pKa = 4.29GDD428 pKa = 2.81ICTQPEE434 pKa = 5.06FIAPCYY440 pKa = 9.2NSNYY444 pKa = 9.85SIPLVVHH451 pKa = 6.52CKK453 pKa = 9.76LHH455 pKa = 6.05NEE457 pKa = 4.39NITGTVLQVMRR468 pKa = 11.84CRR470 pKa = 11.84GMKK473 pKa = 10.47DD474 pKa = 2.83LDD476 pKa = 3.58LRR478 pKa = 11.84IAGEE482 pKa = 4.28FVTLNLTLVKK492 pKa = 10.68DD493 pKa = 3.67PFLDD497 pKa = 3.58YY498 pKa = 11.17LRR500 pKa = 11.84NQVNYY505 pKa = 9.06TCTLNGTFWVYY516 pKa = 11.03KK517 pKa = 9.41SNKK520 pKa = 8.68PKK522 pKa = 9.91WDD524 pKa = 3.74TNEE527 pKa = 3.88TIYY530 pKa = 11.33APVSNFTDD538 pKa = 3.69DD539 pKa = 3.4RR540 pKa = 11.84VVWGAYY546 pKa = 9.96NSILYY551 pKa = 9.55QFYY554 pKa = 11.03ALQKK558 pKa = 10.49FKK560 pKa = 11.07LLKK563 pKa = 10.57KK564 pKa = 9.75PVATIMPPVRR574 pKa = 11.84EE575 pKa = 3.79PHH577 pKa = 6.09RR578 pKa = 11.84RR579 pKa = 11.84QTRR582 pKa = 11.84EE583 pKa = 3.57LRR585 pKa = 11.84QKK587 pKa = 10.53RR588 pKa = 11.84GLGLTIAIVGAVTAGMIGTTTGTAALAVSVRR619 pKa = 11.84LKK621 pKa = 10.99EE622 pKa = 4.36VMLQQAHH629 pKa = 6.72INEE632 pKa = 4.36QVLGALRR639 pKa = 11.84IVQRR643 pKa = 11.84RR644 pKa = 11.84LQDD647 pKa = 3.12AEE649 pKa = 4.27RR650 pKa = 11.84FILSLHH656 pKa = 5.11QRR658 pKa = 11.84VTKK661 pKa = 10.23IEE663 pKa = 4.11RR664 pKa = 11.84FLEE667 pKa = 3.85IQYY670 pKa = 10.05QLQGMCPFKK679 pKa = 10.63DD680 pKa = 3.05ICQLDD685 pKa = 3.66MNFNFTDD692 pKa = 4.3YY693 pKa = 11.23NDD695 pKa = 3.53SWPMGRR701 pKa = 11.84WAEE704 pKa = 3.99QAEE707 pKa = 4.33KK708 pKa = 10.5DD709 pKa = 3.27WRR711 pKa = 11.84EE712 pKa = 3.88FQSLIDD718 pKa = 3.42NATRR722 pKa = 11.84SNEE725 pKa = 3.88NLKK728 pKa = 9.49TDD730 pKa = 3.71LTKK733 pKa = 11.04LEE735 pKa = 4.06VLDD738 pKa = 3.69WFSWFPINSVFSTIFSLIIIIIVVVLARR766 pKa = 11.84PCLEE770 pKa = 3.75NCIKK774 pKa = 10.94GFFSMLKK781 pKa = 9.19GYY783 pKa = 10.45RR784 pKa = 11.84PIKK787 pKa = 9.71LQMVEE792 pKa = 4.25IPLEE796 pKa = 4.25GTTQEE801 pKa = 4.27EE802 pKa = 4.36EE803 pKa = 4.51GEE805 pKa = 4.25EE806 pKa = 4.14EE807 pKa = 4.19DD808 pKa = 5.21NEE810 pKa = 4.85DD811 pKa = 4.69GGEE814 pKa = 4.17SCQTWRR820 pKa = 11.84SDD822 pKa = 4.75LNNCLGKK829 pKa = 10.51KK830 pKa = 10.04KK831 pKa = 10.38SLKK834 pKa = 9.31GLNMHH839 pKa = 7.15HH840 pKa = 7.0

Molecular weight:
96.87 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|Q84809|Q84809_9RETR Exoribonuclease H OS=Puma lentivirus 14 OX=32615 GN=pol PE=3 SV=1
MM1 pKa = 7.66GNNQGKK7 pKa = 7.71EE8 pKa = 3.71LKK10 pKa = 10.24AALRR14 pKa = 11.84RR15 pKa = 11.84ACNVTVGEE23 pKa = 4.51GKK25 pKa = 9.85RR26 pKa = 11.84SKK28 pKa = 10.66RR29 pKa = 11.84YY30 pKa = 8.34TEE32 pKa = 4.49GNLMWAIKK40 pKa = 10.0FGNACTGRR48 pKa = 11.84DD49 pKa = 3.47PADD52 pKa = 3.38VPEE55 pKa = 4.2TLVEE59 pKa = 3.76IRR61 pKa = 11.84NFIHH65 pKa = 6.9EE66 pKa = 4.75LQDD69 pKa = 3.32KK70 pKa = 8.85LQKK73 pKa = 10.68FGGSKK78 pKa = 9.83EE79 pKa = 4.01LEE81 pKa = 4.1NCIKK85 pKa = 9.02TLKK88 pKa = 9.79VLTVAGVLKK97 pKa = 10.26LPCQNTEE104 pKa = 4.07SAIKK108 pKa = 10.31LYY110 pKa = 9.72EE111 pKa = 4.09TMGLLGPATDD121 pKa = 4.96KK122 pKa = 11.15KK123 pKa = 10.69IEE125 pKa = 4.17EE126 pKa = 4.19NLEE129 pKa = 3.98EE130 pKa = 4.87KK131 pKa = 10.15PAEE134 pKa = 4.33AYY136 pKa = 8.52PVQVANGVHH145 pKa = 4.64QHH147 pKa = 5.2VSFNPRR153 pKa = 11.84TAAIWMEE160 pKa = 4.15KK161 pKa = 10.27ARR163 pKa = 11.84GGLGSEE169 pKa = 4.42EE170 pKa = 3.72AVLWFTAFSADD181 pKa = 3.66LTATDD186 pKa = 3.87MASLITAAPGCAADD200 pKa = 5.58KK201 pKa = 10.91KK202 pKa = 10.98IIDD205 pKa = 4.58DD206 pKa = 3.83KK207 pKa = 11.52LKK209 pKa = 10.91EE210 pKa = 4.06LTAKK214 pKa = 10.1YY215 pKa = 10.3AQDD218 pKa = 4.0HH219 pKa = 6.95PDD221 pKa = 3.33GPRR224 pKa = 11.84PLPYY228 pKa = 9.33FTAEE232 pKa = 4.08EE233 pKa = 4.05IMGIGIPQNVQSQPQYY249 pKa = 11.06GPARR253 pKa = 11.84AQARR257 pKa = 11.84LWFLEE262 pKa = 4.09ALGHH266 pKa = 5.05LQKK269 pKa = 10.66IKK271 pKa = 10.69AGEE274 pKa = 4.04PKK276 pKa = 10.45AVTLRR281 pKa = 11.84QGPKK285 pKa = 9.74EE286 pKa = 4.17SYY288 pKa = 10.61KK289 pKa = 11.13DD290 pKa = 3.76FIDD293 pKa = 5.28RR294 pKa = 11.84LFQQIDD300 pKa = 3.33QEE302 pKa = 4.38QASDD306 pKa = 3.68EE307 pKa = 4.09VRR309 pKa = 11.84DD310 pKa = 3.83YY311 pKa = 11.58LKK313 pKa = 10.78QSLSISNANGEE324 pKa = 4.26CRR326 pKa = 11.84KK327 pKa = 10.49AMTHH331 pKa = 6.37LRR333 pKa = 11.84PEE335 pKa = 4.11STLEE339 pKa = 3.8EE340 pKa = 4.32KK341 pKa = 10.73LRR343 pKa = 11.84ACQDD347 pKa = 2.99IGSTQYY353 pKa = 11.27KK354 pKa = 8.14MQMLAEE360 pKa = 5.15AFNQMQVNQVQRR372 pKa = 11.84GGFRR376 pKa = 11.84GGRR379 pKa = 11.84GGNRR383 pKa = 11.84GRR385 pKa = 11.84GGRR388 pKa = 11.84GRR390 pKa = 11.84GRR392 pKa = 11.84GRR394 pKa = 11.84GLGPLNCFNCGKK406 pKa = 9.15PGHH409 pKa = 6.94LASQCRR415 pKa = 11.84QPIKK419 pKa = 10.3CYY421 pKa = 9.11KK422 pKa = 10.09CGGSGHH428 pKa = 7.11LAIDD432 pKa = 3.78CLGGNDD438 pKa = 4.42SKK440 pKa = 11.51NGQNRR445 pKa = 11.84GTAAPRR451 pKa = 11.84QFQVQQNNTLYY462 pKa = 10.19PSLKK466 pKa = 10.26EE467 pKa = 3.97MQTEE471 pKa = 4.23PTAPPMEE478 pKa = 4.27II479 pKa = 3.82

Molecular weight:
52.65 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4

0

4

2681

276

1086

670.3

76.84

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.147 ± 0.983

2.201 ± 0.462

4.998 ± 0.352

6.527 ± 0.433

3.394 ± 0.495

7.162 ± 0.815

1.641 ± 0.097

7.535 ± 1.079

7.46 ± 0.574

9.549 ± 0.404

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.536 ± 0.133

5.259 ± 0.38

5.035 ± 0.149

6.117 ± 0.247

4.924 ± 0.546

3.916 ± 0.196

5.073 ± 0.551

5.334 ± 0.532

2.648 ± 0.687

3.543 ± 0.377

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski