Puma lentivirus 14
Average proteome isoelectric point is 7.17
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|Q84811|Q84811_9RETR Env polyprotein OS=Puma lentivirus 14 OX=32615 GN=env PE=4 SV=1
MM1 pKa = 7.19 AQANPMDD8 pKa = 4.41 NAWIGPEE15 pKa = 3.97 EE16 pKa = 4.98 GEE18 pKa = 4.08 MLLDD22 pKa = 3.84 FEE24 pKa = 5.12 VVMMLHH30 pKa = 6.73 EE31 pKa = 4.72 GSNYY35 pKa = 9.82 PGINPFRR42 pKa = 11.84 DD43 pKa = 3.47 PTLTPEE49 pKa = 4.72 KK50 pKa = 9.89 KK51 pKa = 9.7 QQTAALLQPLLVRR64 pKa = 11.84 IKK66 pKa = 10.87 QNLLQQGKK74 pKa = 8.61 VKK76 pKa = 10.56 NWGKK80 pKa = 10.3 QDD82 pKa = 3.11 WDD84 pKa = 2.91 KK85 pKa = 10.79 MRR87 pKa = 11.84 RR88 pKa = 11.84 YY89 pKa = 9.73 KK90 pKa = 10.71 AGEE93 pKa = 3.83 RR94 pKa = 11.84 AWIKK98 pKa = 9.68 FVKK101 pKa = 10.19 IFGGIHH107 pKa = 5.85 NMPKK111 pKa = 10.43 EE112 pKa = 3.99 EE113 pKa = 4.43 DD114 pKa = 3.44 LGTFSGDD121 pKa = 2.43 IDD123 pKa = 4.4 MITPHH128 pKa = 7.02 SYY130 pKa = 10.83 RR131 pKa = 11.84 KK132 pKa = 8.29 VTYY135 pKa = 8.49 NWKK138 pKa = 8.86 TSLCFFLLIGSAMLAYY154 pKa = 10.56 SLIFTIIIKK163 pKa = 10.01 QALAQAVTLALDD175 pKa = 4.15 PPWVIPLKK183 pKa = 10.47 YY184 pKa = 10.15 RR185 pKa = 11.84 EE186 pKa = 5.18 DD187 pKa = 3.6 INFQCIGNHH196 pKa = 5.85 PEE198 pKa = 3.93 CNLPKK203 pKa = 10.54 EE204 pKa = 4.28 VGDD207 pKa = 3.47 WKK209 pKa = 11.37 QNFTWVYY216 pKa = 5.99 QTPINEE222 pKa = 4.54 TIGLEE227 pKa = 4.03 IYY229 pKa = 10.11 AQEE232 pKa = 3.98 IAAKK236 pKa = 9.97 LWQQVFLQCKK246 pKa = 9.58 KK247 pKa = 10.73 GPRR250 pKa = 11.84 DD251 pKa = 3.41 LDD253 pKa = 3.56 TAIKK257 pKa = 10.35 YY258 pKa = 7.42 WICFYY263 pKa = 10.58 DD264 pKa = 3.57 TAIKK268 pKa = 10.43 YY269 pKa = 10.24 LLGYY273 pKa = 8.01 EE274 pKa = 4.92 HH275 pKa = 7.21 IQLCPLGGYY284 pKa = 9.47 LVYY287 pKa = 11.01 DD288 pKa = 3.69 NTTKK292 pKa = 10.34 EE293 pKa = 4.09 ISMCTPPLSLRR304 pKa = 11.84 LLNFTLSQEE313 pKa = 3.95 KK314 pKa = 9.31 WEE316 pKa = 4.3 QEE318 pKa = 4.0 PFTDD322 pKa = 5.04 IVWFGNKK329 pKa = 9.38 ALNTTVNNITQVQINVTMVCNVIVPEE355 pKa = 3.96 QVGKK359 pKa = 10.77 KK360 pKa = 9.57 KK361 pKa = 10.71 GRR363 pKa = 11.84 FQFYY367 pKa = 11.08 NEE369 pKa = 4.38 FLGPWGGGRR378 pKa = 11.84 FQEE381 pKa = 4.86 IIVRR385 pKa = 11.84 YY386 pKa = 8.38 QDD388 pKa = 2.98 WVNISDD394 pKa = 5.54 PILDD398 pKa = 4.64 LNCSGIPGVDD408 pKa = 4.1 FNHH411 pKa = 6.73 TEE413 pKa = 3.82 GNYY416 pKa = 9.13 TCIHH420 pKa = 6.26 NKK422 pKa = 9.01 TYY424 pKa = 10.49 QEE426 pKa = 4.29 GDD428 pKa = 2.81 ICTQPEE434 pKa = 5.06 FIAPCYY440 pKa = 9.2 NSNYY444 pKa = 9.85 SIPLVVHH451 pKa = 6.52 CKK453 pKa = 9.76 LHH455 pKa = 6.05 NEE457 pKa = 4.39 NITGTVLQVMRR468 pKa = 11.84 CRR470 pKa = 11.84 GMKK473 pKa = 10.47 DD474 pKa = 2.83 LDD476 pKa = 3.58 LRR478 pKa = 11.84 IAGEE482 pKa = 4.28 FVTLNLTLVKK492 pKa = 10.68 DD493 pKa = 3.67 PFLDD497 pKa = 3.58 YY498 pKa = 11.17 LRR500 pKa = 11.84 NQVNYY505 pKa = 9.06 TCTLNGTFWVYY516 pKa = 11.03 KK517 pKa = 9.41 SNKK520 pKa = 8.68 PKK522 pKa = 9.91 WDD524 pKa = 3.74 TNEE527 pKa = 3.88 TIYY530 pKa = 11.33 APVSNFTDD538 pKa = 3.69 DD539 pKa = 3.4 RR540 pKa = 11.84 VVWGAYY546 pKa = 9.96 NSILYY551 pKa = 9.55 QFYY554 pKa = 11.03 ALQKK558 pKa = 10.49 FKK560 pKa = 11.07 LLKK563 pKa = 10.57 KK564 pKa = 9.75 PVATIMPPVRR574 pKa = 11.84 EE575 pKa = 3.79 PHH577 pKa = 6.09 RR578 pKa = 11.84 RR579 pKa = 11.84 QTRR582 pKa = 11.84 EE583 pKa = 3.57 LRR585 pKa = 11.84 QKK587 pKa = 10.53 RR588 pKa = 11.84 GLGLTIAIVGAVTAGMIGTTTGTAALAVSVRR619 pKa = 11.84 LKK621 pKa = 10.99 EE622 pKa = 4.36 VMLQQAHH629 pKa = 6.72 INEE632 pKa = 4.36 QVLGALRR639 pKa = 11.84 IVQRR643 pKa = 11.84 RR644 pKa = 11.84 LQDD647 pKa = 3.12 AEE649 pKa = 4.27 RR650 pKa = 11.84 FILSLHH656 pKa = 5.11 QRR658 pKa = 11.84 VTKK661 pKa = 10.23 IEE663 pKa = 4.11 RR664 pKa = 11.84 FLEE667 pKa = 3.85 IQYY670 pKa = 10.05 QLQGMCPFKK679 pKa = 10.63 DD680 pKa = 3.05 ICQLDD685 pKa = 3.66 MNFNFTDD692 pKa = 4.3 YY693 pKa = 11.23 NDD695 pKa = 3.53 SWPMGRR701 pKa = 11.84 WAEE704 pKa = 3.99 QAEE707 pKa = 4.33 KK708 pKa = 10.5 DD709 pKa = 3.27 WRR711 pKa = 11.84 EE712 pKa = 3.88 FQSLIDD718 pKa = 3.42 NATRR722 pKa = 11.84 SNEE725 pKa = 3.88 NLKK728 pKa = 9.49 TDD730 pKa = 3.71 LTKK733 pKa = 11.04 LEE735 pKa = 4.06 VLDD738 pKa = 3.69 WFSWFPINSVFSTIFSLIIIIIVVVLARR766 pKa = 11.84 PCLEE770 pKa = 3.75 NCIKK774 pKa = 10.94 GFFSMLKK781 pKa = 9.19 GYY783 pKa = 10.45 RR784 pKa = 11.84 PIKK787 pKa = 9.71 LQMVEE792 pKa = 4.25 IPLEE796 pKa = 4.25 GTTQEE801 pKa = 4.27 EE802 pKa = 4.36 EE803 pKa = 4.51 GEE805 pKa = 4.25 EE806 pKa = 4.14 EE807 pKa = 4.19 DD808 pKa = 5.21 NEE810 pKa = 4.85 DD811 pKa = 4.69 GGEE814 pKa = 4.17 SCQTWRR820 pKa = 11.84 SDD822 pKa = 4.75 LNNCLGKK829 pKa = 10.51 KK830 pKa = 10.04 KK831 pKa = 10.38 SLKK834 pKa = 9.31 GLNMHH839 pKa = 7.15 HH840 pKa = 7.0
Molecular weight: 96.87 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 6.049
IPC2_protein 6.16
IPC_protein 6.211
Toseland 6.326
ProMoST 6.517
Dawson 6.561
Bjellqvist 6.576
Wikipedia 6.561
Rodwell 6.547
Grimsley 6.402
Solomon 6.561
Lehninger 6.561
Nozaki 6.854
DTASelect 6.971
Thurlkill 7.0
EMBOSS 6.971
Sillero 6.956
Patrickios 4.062
IPC_peptide 6.576
IPC2_peptide 6.737
IPC2.peptide.svr19 6.74
Protein with the highest isoelectric point:
>tr|Q84809|Q84809_9RETR Exoribonuclease H OS=Puma lentivirus 14 OX=32615 GN=pol PE=3 SV=1
MM1 pKa = 7.66 GNNQGKK7 pKa = 7.71 EE8 pKa = 3.71 LKK10 pKa = 10.24 AALRR14 pKa = 11.84 RR15 pKa = 11.84 ACNVTVGEE23 pKa = 4.51 GKK25 pKa = 9.85 RR26 pKa = 11.84 SKK28 pKa = 10.66 RR29 pKa = 11.84 YY30 pKa = 8.34 TEE32 pKa = 4.49 GNLMWAIKK40 pKa = 10.0 FGNACTGRR48 pKa = 11.84 DD49 pKa = 3.47 PADD52 pKa = 3.38 VPEE55 pKa = 4.2 TLVEE59 pKa = 3.76 IRR61 pKa = 11.84 NFIHH65 pKa = 6.9 EE66 pKa = 4.75 LQDD69 pKa = 3.32 KK70 pKa = 8.85 LQKK73 pKa = 10.68 FGGSKK78 pKa = 9.83 EE79 pKa = 4.01 LEE81 pKa = 4.1 NCIKK85 pKa = 9.02 TLKK88 pKa = 9.79 VLTVAGVLKK97 pKa = 10.26 LPCQNTEE104 pKa = 4.07 SAIKK108 pKa = 10.31 LYY110 pKa = 9.72 EE111 pKa = 4.09 TMGLLGPATDD121 pKa = 4.96 KK122 pKa = 11.15 KK123 pKa = 10.69 IEE125 pKa = 4.17 EE126 pKa = 4.19 NLEE129 pKa = 3.98 EE130 pKa = 4.87 KK131 pKa = 10.15 PAEE134 pKa = 4.33 AYY136 pKa = 8.52 PVQVANGVHH145 pKa = 4.64 QHH147 pKa = 5.2 VSFNPRR153 pKa = 11.84 TAAIWMEE160 pKa = 4.15 KK161 pKa = 10.27 ARR163 pKa = 11.84 GGLGSEE169 pKa = 4.42 EE170 pKa = 3.72 AVLWFTAFSADD181 pKa = 3.66 LTATDD186 pKa = 3.87 MASLITAAPGCAADD200 pKa = 5.58 KK201 pKa = 10.91 KK202 pKa = 10.98 IIDD205 pKa = 4.58 DD206 pKa = 3.83 KK207 pKa = 11.52 LKK209 pKa = 10.91 EE210 pKa = 4.06 LTAKK214 pKa = 10.1 YY215 pKa = 10.3 AQDD218 pKa = 4.0 HH219 pKa = 6.95 PDD221 pKa = 3.33 GPRR224 pKa = 11.84 PLPYY228 pKa = 9.33 FTAEE232 pKa = 4.08 EE233 pKa = 4.05 IMGIGIPQNVQSQPQYY249 pKa = 11.06 GPARR253 pKa = 11.84 AQARR257 pKa = 11.84 LWFLEE262 pKa = 4.09 ALGHH266 pKa = 5.05 LQKK269 pKa = 10.66 IKK271 pKa = 10.69 AGEE274 pKa = 4.04 PKK276 pKa = 10.45 AVTLRR281 pKa = 11.84 QGPKK285 pKa = 9.74 EE286 pKa = 4.17 SYY288 pKa = 10.61 KK289 pKa = 11.13 DD290 pKa = 3.76 FIDD293 pKa = 5.28 RR294 pKa = 11.84 LFQQIDD300 pKa = 3.33 QEE302 pKa = 4.38 QASDD306 pKa = 3.68 EE307 pKa = 4.09 VRR309 pKa = 11.84 DD310 pKa = 3.83 YY311 pKa = 11.58 LKK313 pKa = 10.78 QSLSISNANGEE324 pKa = 4.26 CRR326 pKa = 11.84 KK327 pKa = 10.49 AMTHH331 pKa = 6.37 LRR333 pKa = 11.84 PEE335 pKa = 4.11 STLEE339 pKa = 3.8 EE340 pKa = 4.32 KK341 pKa = 10.73 LRR343 pKa = 11.84 ACQDD347 pKa = 2.99 IGSTQYY353 pKa = 11.27 KK354 pKa = 8.14 MQMLAEE360 pKa = 5.15 AFNQMQVNQVQRR372 pKa = 11.84 GGFRR376 pKa = 11.84 GGRR379 pKa = 11.84 GGNRR383 pKa = 11.84 GRR385 pKa = 11.84 GGRR388 pKa = 11.84 GRR390 pKa = 11.84 GRR392 pKa = 11.84 GRR394 pKa = 11.84 GLGPLNCFNCGKK406 pKa = 9.15 PGHH409 pKa = 6.94 LASQCRR415 pKa = 11.84 QPIKK419 pKa = 10.3 CYY421 pKa = 9.11 KK422 pKa = 10.09 CGGSGHH428 pKa = 7.11 LAIDD432 pKa = 3.78 CLGGNDD438 pKa = 4.42 SKK440 pKa = 11.51 NGQNRR445 pKa = 11.84 GTAAPRR451 pKa = 11.84 QFQVQQNNTLYY462 pKa = 10.19 PSLKK466 pKa = 10.26 EE467 pKa = 3.97 MQTEE471 pKa = 4.23 PTAPPMEE478 pKa = 4.27 II479 pKa = 3.82
Molecular weight: 52.65 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 7.837
IPC2_protein 8.053
IPC_protein 7.878
Toseland 7.644
ProMoST 8.492
Dawson 8.697
Bjellqvist 8.96
Wikipedia 8.639
Rodwell 8.77
Grimsley 7.6
Solomon 8.77
Lehninger 8.785
Nozaki 9.224
DTASelect 8.697
Thurlkill 8.799
EMBOSS 8.902
Sillero 9.092
Patrickios 4.698
IPC_peptide 8.77
IPC2_peptide 7.775
IPC2.peptide.svr19 7.951
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4
0
4
2681
276
1086
670.3
76.84
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.147 ± 0.983
2.201 ± 0.462
4.998 ± 0.352
6.527 ± 0.433
3.394 ± 0.495
7.162 ± 0.815
1.641 ± 0.097
7.535 ± 1.079
7.46 ± 0.574
9.549 ± 0.404
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.536 ± 0.133
5.259 ± 0.38
5.035 ± 0.149
6.117 ± 0.247
4.924 ± 0.546
3.916 ± 0.196
5.073 ± 0.551
5.334 ± 0.532
2.648 ± 0.687
3.543 ± 0.377
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here