Strigops habroptila (Kakapo)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Deuterostomia; Chordata; Craniata; Vertebrata; Gnathostomata; Teleostomi; Euteleostomi; Sarcopterygii; Dipnotetrapodomorpha; Tetrapoda; Amniota; Sauropsida; Sauria; Archelosauria;

Average proteome isoelectric point is 6.65

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 24357 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A672U9N9|A0A672U9N9_STRHB Serine/threonine-protein kinase PDIK1L-like OS=Strigops habroptila OX=2489341 GN=LOC115610042 PE=3 SV=1
MM1 pKa = 6.75YY2 pKa = 10.03QCPCPVISIIYY13 pKa = 9.39CEE15 pKa = 4.09QIANFTYY22 pKa = 8.16PTDD25 pKa = 4.21WIDD28 pKa = 3.31CRR30 pKa = 11.84YY31 pKa = 9.87EE32 pKa = 3.99GLIVTSDD39 pKa = 3.37SSDD42 pKa = 3.44EE43 pKa = 3.98EE44 pKa = 4.81LPFLLEE50 pKa = 5.0APAEE54 pKa = 4.16QQRR57 pKa = 11.84EE58 pKa = 4.17EE59 pKa = 4.34EE60 pKa = 4.58DD61 pKa = 3.79DD62 pKa = 3.83DD63 pKa = 4.89VVILEE68 pKa = 4.61EE69 pKa = 4.33DD70 pKa = 3.41EE71 pKa = 4.53NMNFALPQSRR81 pKa = 11.84SSEE84 pKa = 3.96LNGPGFEE91 pKa = 4.14LTSNQEE97 pKa = 4.17PGPSLLPPVTTNPQPVVNQEE117 pKa = 3.99IISLEE122 pKa = 4.04NSDD125 pKa = 5.65LPPQQGEE132 pKa = 4.28KK133 pKa = 10.68VEE135 pKa = 4.1AQQGEE140 pKa = 4.38DD141 pKa = 4.61LEE143 pKa = 4.94VEE145 pKa = 4.16QNLRR149 pKa = 11.84EE150 pKa = 4.13PADD153 pKa = 4.19AGPADD158 pKa = 3.94QEE160 pKa = 4.36THH162 pKa = 6.53EE163 pKa = 4.71NSQLDD168 pKa = 3.82HH169 pKa = 7.29PYY171 pKa = 9.61VQPLEE176 pKa = 4.52GEE178 pKa = 3.88PHH180 pKa = 6.21AVVNSCAPQQGQPEE194 pKa = 4.49ADD196 pKa = 3.62PGSLRR201 pKa = 11.84AYY203 pKa = 9.98GEE205 pKa = 4.3KK206 pKa = 10.68APGPAFPRR214 pKa = 11.84PEE216 pKa = 4.13PQQDD220 pKa = 4.72GIPGPASPQPAHH232 pKa = 6.69PPEE235 pKa = 4.3EE236 pKa = 4.42TGPQAAVDD244 pKa = 3.88NEE246 pKa = 4.42QPGPAFPAQGCQEE259 pKa = 4.43LGSSNVPEE267 pKa = 4.02QGAAGQDD274 pKa = 3.3QDD276 pKa = 4.63LTAQLIRR283 pKa = 11.84EE284 pKa = 4.36TVSIPRR290 pKa = 11.84ACLPSVKK297 pKa = 10.31GIPP300 pKa = 3.38

Molecular weight: 32.36 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A672UVS3|A0A672UVS3_STRHB PDZ and LIM domain 7 OS=Strigops habroptila OX=2489341 GN=PDLIM7 PE=4 SV=1
MM1 pKa = 7.57SSHH4 pKa = 6.01KK5 pKa = 9.07TFKK8 pKa = 10.28IKK10 pKa = 10.6RR11 pKa = 11.84FLAKK15 pKa = 9.68KK16 pKa = 9.58QKK18 pKa = 8.69QNRR21 pKa = 11.84PIPQWIRR28 pKa = 11.84MKK30 pKa = 9.89TGNKK34 pKa = 8.61IRR36 pKa = 11.84YY37 pKa = 7.09NSKK40 pKa = 8.3RR41 pKa = 11.84RR42 pKa = 11.84HH43 pKa = 3.95WRR45 pKa = 11.84RR46 pKa = 11.84TKK48 pKa = 10.83LGLL51 pKa = 3.67

Molecular weight: 6.38 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

16021

8336

24357

16680854

20

8784

684.8

76.57

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.811 ± 0.014

2.283 ± 0.013

4.99 ± 0.01

7.098 ± 0.019

3.721 ± 0.01

6.211 ± 0.017

2.544 ± 0.006

4.741 ± 0.012

5.983 ± 0.015

9.776 ± 0.02

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.257 ± 0.006

3.958 ± 0.01

5.652 ± 0.019

4.725 ± 0.015

5.411 ± 0.011

8.3 ± 0.019

5.34 ± 0.01

6.158 ± 0.012

1.207 ± 0.005

2.834 ± 0.01
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski