Candidatus Afipia apatlaquensis

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales; Bradyrhizobiaceae; Afipia; unclassified Afipia

Average proteome isoelectric point is 6.94

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6186 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A7C9RF33|A0A7C9RF33_9BRAD Uncharacterized protein (Fragment) OS=Candidatus Afipia apatlaquensis OX=2712852 GN=G4V63_10955 PE=4 SV=1
MM1 pKa = 6.75LTARR5 pKa = 11.84AMRR8 pKa = 11.84FPLIVTMAVIIAAATLPSTDD28 pKa = 2.77AHH30 pKa = 6.15AQFACVGTLGASDD43 pKa = 3.83VTCTNTGATAVPFLQDD59 pKa = 3.17QLGTFNLTTTSSGLTNGITTTSVDD83 pKa = 3.74GNVTATNSGINADD96 pKa = 4.13MLDD99 pKa = 3.69TSASGLGNATSNNSGIIGTYY119 pKa = 9.95ISTLTGAGNATSTNSGTVLQDD140 pKa = 2.96ITTSAQDD147 pKa = 3.29GGNALTNNSGSTFAITTSTVPGSALVSGSATTNNSGTVGGSVTTTADD194 pKa = 2.96GGGNATTNNSGVIGGLLSTQTSNGGSGSGGGNATSINSGTVDD236 pKa = 3.87SIANVTADD244 pKa = 3.69GGNATVTNSGNVLFNGCCGPPFGIFNGTALGGDD277 pKa = 3.49ATTINYY283 pKa = 9.89GNVNGGVFTTTGLGGGDD300 pKa = 3.66GNATLINYY308 pKa = 7.27GTIRR312 pKa = 11.84GDD314 pKa = 3.65VLTLAGNTGGTGNATFVNYY333 pKa = 8.2GTIYY337 pKa = 10.57TDD339 pKa = 3.65FLGVGVGAYY348 pKa = 9.98NGTVTGYY355 pKa = 10.13NGGRR359 pKa = 11.84IFGEE363 pKa = 3.56VDD365 pKa = 3.21VANGGGGGTVVFSNAGLIDD384 pKa = 3.62GTYY387 pKa = 9.97GLSPTGVALDD397 pKa = 3.94LTQFDD402 pKa = 4.53PATPTTLNILPGSRR416 pKa = 11.84IVGEE420 pKa = 4.35IILNGDD426 pKa = 3.24TSDD429 pKa = 3.64PFAVGTRR436 pKa = 11.84VNILSGHH443 pKa = 6.95DD444 pKa = 3.19ISSVLTFGGSGCGCTTGGLIDD465 pKa = 3.69TGAVVNVTGGAPYY478 pKa = 10.16VINGNTVAILDD489 pKa = 3.83PTSFANADD497 pKa = 3.96RR498 pKa = 11.84NVVDD502 pKa = 3.55VTRR505 pKa = 11.84TIASLVTSRR514 pKa = 11.84LTNPP518 pKa = 3.64

Molecular weight: 50.31 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A7C9VL65|A0A7C9VL65_9BRAD Thermonuclease family protein OS=Candidatus Afipia apatlaquensis OX=2712852 GN=G4V63_12865 PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 9.43RR3 pKa = 11.84TYY5 pKa = 10.27QPSKK9 pKa = 9.73LVRR12 pKa = 11.84KK13 pKa = 9.15RR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.55GFRR19 pKa = 11.84ARR21 pKa = 11.84LATAGGRR28 pKa = 11.84KK29 pKa = 8.95VLAARR34 pKa = 11.84RR35 pKa = 11.84ARR37 pKa = 11.84GRR39 pKa = 11.84KK40 pKa = 9.03RR41 pKa = 11.84LSAA44 pKa = 4.03

Molecular weight: 5.09 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6186

0

6186

1551791

19

2645

250.9

27.26

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.078 ± 0.041

0.833 ± 0.011

5.57 ± 0.03

5.262 ± 0.031

3.84 ± 0.022

8.266 ± 0.035

1.963 ± 0.015

5.665 ± 0.024

4.054 ± 0.028

9.692 ± 0.039

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.486 ± 0.016

2.938 ± 0.019

5.131 ± 0.027

3.177 ± 0.018

6.724 ± 0.038

5.788 ± 0.025

5.49 ± 0.025

7.457 ± 0.029

1.298 ± 0.013

2.291 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski