Amino acid dipepetide frequency for Mariprofundus sp. EBB-1

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	9.723AlaAla: 9.723 ± 0.172	1.247AlaCys: 1.247 ± 0.042	5.725AlaAsp: 5.725 ± 0.086	5.917AlaGlu: 5.917 ± 0.09	3.58AlaPhe: 3.58 ± 0.078	7.572AlaGly: 7.572 ± 0.128	2.225AlaHis: 2.225 ± 0.06	6.047AlaIle: 6.047 ± 0.087	4.21AlaLys: 4.21 ± 0.085	10.54AlaLeu: 10.54 ± 0.146	3.293AlaMet: 3.293 ± 0.068	3.19AlaAsn: 3.19 ± 0.068	3.087AlaPro: 3.087 ± 0.068	3.327AlaGln: 3.327 ± 0.064	4.649AlaArg: 4.649 ± 0.083	6.304AlaSer: 6.304 ± 0.099	4.806AlaThr: 4.806 ± 0.103	6.8AlaVal: 6.8 ± 0.112	1.336AlaTrp: 1.336 ± 0.041	2.499AlaTyr: 2.499 ± 0.06	0.0AlaXaa: 0.0 ± 0.0
Cys	0.879CysAla: 0.879 ± 0.038	0.216CysCys: 0.216 ± 0.018	0.682CysAsp: 0.682 ± 0.032	0.655CysGlu: 0.655 ± 0.034	0.466CysPhe: 0.466 ± 0.022	1.005CysGly: 1.005 ± 0.039	0.376CysHis: 0.376 ± 0.023	0.731CysIle: 0.731 ± 0.029	0.508CysLys: 0.508 ± 0.028	1.068CysLeu: 1.068 ± 0.044	0.35CysMet: 0.35 ± 0.021	0.429CysAsn: 0.429 ± 0.026	0.55CysPro: 0.55 ± 0.029	0.361CysGln: 0.361 ± 0.022	0.529CysArg: 0.529 ± 0.025	0.739CysSer: 0.739 ± 0.031	0.489CysThr: 0.489 ± 0.027	0.654CysVal: 0.654 ± 0.033	0.154CysTrp: 0.154 ± 0.013	0.284CysTyr: 0.284 ± 0.018	0.0CysXaa: 0.0 ± 0.0
Asp	6.475AspAla: 6.475 ± 0.106	0.595AspCys: 0.595 ± 0.029	3.458AspAsp: 3.458 ± 0.065	3.771AspGlu: 3.771 ± 0.077	2.158AspPhe: 2.158 ± 0.058	4.331AspGly: 4.331 ± 0.083	1.575AspHis: 1.575 ± 0.038	4.042AspIle: 4.042 ± 0.067	2.594AspLys: 2.594 ± 0.053	5.286AspLeu: 5.286 ± 0.079	1.783AspMet: 1.783 ± 0.046	1.877AspAsn: 1.877 ± 0.05	2.204AspPro: 2.204 ± 0.058	2.296AspGln: 2.296 ± 0.057	2.774AspArg: 2.774 ± 0.053	3.156AspSer: 3.156 ± 0.063	2.665AspThr: 2.665 ± 0.064	4.499AspVal: 4.499 ± 0.089	0.804AspTrp: 0.804 ± 0.034	1.757AspTyr: 1.757 ± 0.05	0.0AspXaa: 0.0 ± 0.0
Glu	5.829GluAla: 5.829 ± 0.092	0.52GluCys: 0.52 ± 0.027	2.874GluAsp: 2.874 ± 0.058	3.28GluGlu: 3.28 ± 0.078	1.872GluPhe: 1.872 ± 0.052	3.633GluGly: 3.633 ± 0.073	1.856GluHis: 1.856 ± 0.052	3.702GluIle: 3.702 ± 0.073	3.49GluLys: 3.49 ± 0.077	5.926GluLeu: 5.926 ± 0.104	1.968GluMet: 1.968 ± 0.047	2.143GluAsn: 2.143 ± 0.052	2.068GluPro: 2.068 ± 0.053	3.228GluGln: 3.228 ± 0.068	3.549GluArg: 3.549 ± 0.079	3.848GluSer: 3.848 ± 0.074	3.186GluThr: 3.186 ± 0.062	3.929GluVal: 3.929 ± 0.081	0.72GluTrp: 0.72 ± 0.031	1.407GluTyr: 1.407 ± 0.042	0.0GluXaa: 0.0 ± 0.0
Phe	3.296PheAla: 3.296 ± 0.067	0.478PheCys: 0.478 ± 0.026	2.627PheAsp: 2.627 ± 0.067	2.365PheGlu: 2.365 ± 0.057	1.524PhePhe: 1.524 ± 0.05	2.899PheGly: 2.899 ± 0.061	0.975PheHis: 0.975 ± 0.032	2.306PheIle: 2.306 ± 0.057	1.617PheLys: 1.617 ± 0.047	3.239PheLeu: 3.239 ± 0.08	1.014PheMet: 1.014 ± 0.039	1.585PheAsn: 1.585 ± 0.039	1.478PhePro: 1.478 ± 0.043	1.135PheGln: 1.135 ± 0.038	1.744PheArg: 1.744 ± 0.044	3.006PheSer: 3.006 ± 0.053	2.068PheThr: 2.068 ± 0.055	2.437PheVal: 2.437 ± 0.059	0.478PheTrp: 0.478 ± 0.024	1.093PheTyr: 1.093 ± 0.037	0.0PheXaa: 0.0 ± 0.0
Gly	5.859GlyAla: 5.859 ± 0.114	0.958GlyCys: 0.958 ± 0.037	4.173GlyAsp: 4.173 ± 0.075	4.191GlyGlu: 4.191 ± 0.074	3.118GlyPhe: 3.118 ± 0.071	5.269GlyGly: 5.269 ± 0.112	1.963GlyHis: 1.963 ± 0.046	4.788GlyIle: 4.788 ± 0.087	4.094GlyLys: 4.094 ± 0.08	7.415GlyLeu: 7.415 ± 0.121	2.527GlyMet: 2.527 ± 0.055	2.707GlyAsn: 2.707 ± 0.078	1.864GlyPro: 1.864 ± 0.048	2.47GlyGln: 2.47 ± 0.059	3.524GlyArg: 3.524 ± 0.077	4.553GlySer: 4.553 ± 0.084	3.477GlyThr: 3.477 ± 0.093	5.28GlyVal: 5.28 ± 0.093	1.085GlyTrp: 1.085 ± 0.038	2.106GlyTyr: 2.106 ± 0.059	0.0GlyXaa: 0.0 ± 0.0
His	2.817HisAla: 2.817 ± 0.061	0.383HisCys: 0.383 ± 0.023	1.57HisAsp: 1.57 ± 0.043	1.464HisGlu: 1.464 ± 0.041	1.121HisPhe: 1.121 ± 0.037	2.127HisGly: 2.127 ± 0.055	0.943HisHis: 0.943 ± 0.039	1.886HisIle: 1.886 ± 0.051	1.061HisLys: 1.061 ± 0.034	2.691HisLeu: 2.691 ± 0.069	0.772HisMet: 0.772 ± 0.029	0.853HisAsn: 0.853 ± 0.036	1.377HisPro: 1.377 ± 0.038	1.232HisGln: 1.232 ± 0.043	1.361HisArg: 1.361 ± 0.048	1.443HisSer: 1.443 ± 0.036	1.248HisThr: 1.248 ± 0.039	1.818HisVal: 1.818 ± 0.048	0.384HisTrp: 0.384 ± 0.023	0.856HisTyr: 0.856 ± 0.035	0.0HisXaa: 0.0 ± 0.0
Ile	6.698IleAla: 6.698 ± 0.099	0.748IleCys: 0.748 ± 0.034	4.115IleAsp: 4.115 ± 0.081	4.521IleGlu: 4.521 ± 0.085	1.993IlePhe: 1.993 ± 0.045	4.763IleGly: 4.763 ± 0.081	1.696IleHis: 1.696 ± 0.042	3.851IleIle: 3.851 ± 0.076	3.043IleLys: 3.043 ± 0.062	5.421IleLeu: 5.421 ± 0.103	1.426IleMet: 1.426 ± 0.038	2.58IleAsn: 2.58 ± 0.065	2.638IlePro: 2.638 ± 0.054	2.26IleGln: 2.26 ± 0.062	3.431IleArg: 3.431 ± 0.064	4.475IleSer: 4.475 ± 0.083	3.684IleThr: 3.684 ± 0.097	4.1IleVal: 4.1 ± 0.085	0.666IleTrp: 0.666 ± 0.032	1.569IleTyr: 1.569 ± 0.044	0.0IleXaa: 0.0 ± 0.0
Lys	4.845LysAla: 4.845 ± 0.09	0.321LysCys: 0.321 ± 0.02	2.429LysAsp: 2.429 ± 0.062	2.865LysGlu: 2.865 ± 0.064	1.175LysPhe: 1.175 ± 0.042	3.17LysGly: 3.17 ± 0.068	1.431LysHis: 1.431 ± 0.037	2.634LysIle: 2.634 ± 0.061	3.029LysLys: 3.029 ± 0.083	4.76LysLeu: 4.76 ± 0.079	1.441LysMet: 1.441 ± 0.048	1.897LysAsn: 1.897 ± 0.041	2.334LysPro: 2.334 ± 0.052	2.714LysGln: 2.714 ± 0.066	3.009LysArg: 3.009 ± 0.066	3.021LysSer: 3.021 ± 0.064	2.6LysThr: 2.6 ± 0.066	3.188LysVal: 3.188 ± 0.066	0.437LysTrp: 0.437 ± 0.024	1.089LysTyr: 1.089 ± 0.032	0.0LysXaa: 0.0 ± 0.0
Leu	10.167LeuAla: 10.167 ± 0.145	1.151LeuCys: 1.151 ± 0.04	5.754LeuAsp: 5.754 ± 0.093	5.641LeuGlu: 5.641 ± 0.084	4.011LeuPhe: 4.011 ± 0.075	6.6LeuGly: 6.6 ± 0.107	2.755LeuHis: 2.755 ± 0.059	6.415LeuIle: 6.415 ± 0.096	5.292LeuLys: 5.292 ± 0.098	11.34LeuLeu: 11.34 ± 0.169	3.1LeuMet: 3.1 ± 0.056	4.166LeuAsn: 4.166 ± 0.078	4.707LeuPro: 4.707 ± 0.069	4.218LeuGln: 4.218 ± 0.09	5.108LeuArg: 5.108 ± 0.082	7.617LeuSer: 7.617 ± 0.106	5.237LeuThr: 5.237 ± 0.086	5.945LeuVal: 5.945 ± 0.105	1.186LeuTrp: 1.186 ± 0.041	2.369LeuTyr: 2.369 ± 0.05	0.0LeuXaa: 0.0 ± 0.0
Met	2.919MetAla: 2.919 ± 0.065	0.257MetCys: 0.257 ± 0.018	1.71MetAsp: 1.71 ± 0.047	1.375MetGlu: 1.375 ± 0.043	1.015MetPhe: 1.015 ± 0.035	1.968MetGly: 1.968 ± 0.051	0.938MetHis: 0.938 ± 0.032	1.902MetIle: 1.902 ± 0.049	1.611MetLys: 1.611 ± 0.049	3.507MetLeu: 3.507 ± 0.065	1.042MetMet: 1.042 ± 0.037	1.316MetAsn: 1.316 ± 0.039	1.6MetPro: 1.6 ± 0.04	1.83MetGln: 1.83 ± 0.051	1.831MetArg: 1.831 ± 0.05	1.908MetSer: 1.908 ± 0.046	1.534MetThr: 1.534 ± 0.043	1.968MetVal: 1.968 ± 0.05	0.237MetTrp: 0.237 ± 0.016	0.501MetTyr: 0.501 ± 0.025	0.0MetXaa: 0.0 ± 0.0
Asn	3.533AsnAla: 3.533 ± 0.072	0.354AsnCys: 0.354 ± 0.018	2.097AsnAsp: 2.097 ± 0.05	1.867AsnGlu: 1.867 ± 0.05	1.13AsnPhe: 1.13 ± 0.037	2.649AsnGly: 2.649 ± 0.067	0.973AsnHis: 0.973 ± 0.033	2.811AsnIle: 2.811 ± 0.075	1.857AsnLys: 1.857 ± 0.043	3.399AsnLeu: 3.399 ± 0.071	1.134AsnMet: 1.134 ± 0.034	1.677AsnAsn: 1.677 ± 0.055	1.894AsnPro: 1.894 ± 0.046	1.517AsnGln: 1.517 ± 0.042	2.101AsnArg: 2.101 ± 0.059	2.12AsnSer: 2.12 ± 0.054	2.028AsnThr: 2.028 ± 0.057	2.474AsnVal: 2.474 ± 0.065	0.502AsnTrp: 0.502 ± 0.027	0.964AsnTyr: 0.964 ± 0.032	0.0AsnXaa: 0.0 ± 0.0
Pro	3.912ProAla: 3.912 ± 0.087	0.414ProCys: 0.414 ± 0.027	2.95ProAsp: 2.95 ± 0.056	3.177ProGlu: 3.177 ± 0.06	1.738ProPhe: 1.738 ± 0.046	2.744ProGly: 2.744 ± 0.057	0.919ProHis: 0.919 ± 0.031	2.174ProIle: 2.174 ± 0.055	1.712ProLys: 1.712 ± 0.047	3.843ProLeu: 3.843 ± 0.067	1.215ProMet: 1.215 ± 0.04	1.367ProAsn: 1.367 ± 0.046	1.219ProPro: 1.219 ± 0.044	1.335ProGln: 1.335 ± 0.041	1.525ProArg: 1.525 ± 0.042	2.343ProSer: 2.343 ± 0.05	1.905ProThr: 1.905 ± 0.054	3.586ProVal: 3.586 ± 0.07	0.541ProTrp: 0.541 ± 0.027	1.039ProTyr: 1.039 ± 0.035	0.0ProXaa: 0.0 ± 0.0
Gln	4.43GlnAla: 4.43 ± 0.081	0.442GlnCys: 0.442 ± 0.025	1.73GlnAsp: 1.73 ± 0.046	2.032GlnGlu: 2.032 ± 0.053	1.353GlnPhe: 1.353 ± 0.04	2.714GlnGly: 2.714 ± 0.057	1.454GlnHis: 1.454 ± 0.044	2.317GlnIle: 2.317 ± 0.048	1.877GlnLys: 1.877 ± 0.045	4.534GlnLeu: 4.534 ± 0.102	1.301GlnMet: 1.301 ± 0.039	1.265GlnAsn: 1.265 ± 0.045	1.545GlnPro: 1.545 ± 0.052	2.909GlnGln: 2.909 ± 0.077	2.555GlnArg: 2.555 ± 0.056	2.693GlnSer: 2.693 ± 0.064	2.081GlnThr: 2.081 ± 0.06	2.787GlnVal: 2.787 ± 0.063	0.658GlnTrp: 0.658 ± 0.03	1.076GlnTyr: 1.076 ± 0.04	0.0GlnXaa: 0.0 ± 0.0
Arg	4.138ArgAla: 4.138 ± 0.074	0.559ArgCys: 0.559 ± 0.03	3.009ArgAsp: 3.009 ± 0.055	3.137ArgGlu: 3.137 ± 0.069	2.316ArgPhe: 2.316 ± 0.052	3.019ArgGly: 3.019 ± 0.062	1.465ArgHis: 1.465 ± 0.048	3.669ArgIle: 3.669 ± 0.078	2.725ArgLys: 2.725 ± 0.065	5.968ArgLeu: 5.968 ± 0.097	1.822ArgMet: 1.822 ± 0.044	1.887ArgAsn: 1.887 ± 0.045	1.787ArgPro: 1.787 ± 0.048	2.375ArgGln: 2.375 ± 0.065	2.775ArgArg: 2.775 ± 0.067	3.202ArgSer: 3.202 ± 0.075	2.218ArgThr: 2.218 ± 0.044	3.445ArgVal: 3.445 ± 0.071	0.804ArgTrp: 0.804 ± 0.037	1.628ArgTyr: 1.628 ± 0.054	0.0ArgXaa: 0.0 ± 0.0
Ser	5.808SerAla: 5.808 ± 0.096	0.74SerCys: 0.74 ± 0.029	4.053SerAsp: 4.053 ± 0.076	3.942SerGlu: 3.942 ± 0.079	2.628SerPhe: 2.628 ± 0.062	5.467SerGly: 5.467 ± 0.101	1.655SerHis: 1.655 ± 0.05	4.255SerIle: 4.255 ± 0.079	2.877SerLys: 2.877 ± 0.068	6.562SerLeu: 6.562 ± 0.091	2.255SerMet: 2.255 ± 0.054	2.42SerAsn: 2.42 ± 0.064	2.466SerPro: 2.466 ± 0.055	2.303SerGln: 2.303 ± 0.052	3.137SerArg: 3.137 ± 0.064	4.477SerSer: 4.477 ± 0.101	3.265SerThr: 3.265 ± 0.07	4.323SerVal: 4.323 ± 0.087	0.844SerTrp: 0.844 ± 0.03	1.736SerTyr: 1.736 ± 0.05	0.0SerXaa: 0.0 ± 0.0
Thr	5.029ThrAla: 5.029 ± 0.115	0.503ThrCys: 0.503 ± 0.023	2.853ThrAsp: 2.853 ± 0.061	2.667ThrGlu: 2.667 ± 0.066	1.893ThrPhe: 1.893 ± 0.047	4.264ThrGly: 4.264 ± 0.087	1.34ThrHis: 1.34 ± 0.04	3.235ThrIle: 3.235 ± 0.081	1.913ThrLys: 1.913 ± 0.048	5.705ThrLeu: 5.705 ± 0.103	1.368ThrMet: 1.368 ± 0.04	1.649ThrAsn: 1.649 ± 0.059	2.53ThrPro: 2.53 ± 0.06	1.788ThrGln: 1.788 ± 0.049	2.259ThrArg: 2.259 ± 0.054	3.11ThrSer: 3.11 ± 0.076	2.911ThrThr: 2.911 ± 0.095	3.77ThrVal: 3.77 ± 0.109	0.57ThrTrp: 0.57 ± 0.031	1.344ThrTyr: 1.344 ± 0.046	0.0ThrXaa: 0.0 ± 0.0
Val	6.449ValAla: 6.449 ± 0.107	0.82ValCys: 0.82 ± 0.033	4.154ValAsp: 4.154 ± 0.069	4.277ValGlu: 4.277 ± 0.088	2.544ValPhe: 2.544 ± 0.059	4.533ValGly: 4.533 ± 0.087	1.681ValHis: 1.681 ± 0.041	4.638ValIle: 4.638 ± 0.083	3.043ValLys: 3.043 ± 0.072	6.975ValLeu: 6.975 ± 0.106	2.033ValMet: 2.033 ± 0.057	2.638ValAsn: 2.638 ± 0.077	2.662ValPro: 2.662 ± 0.053	2.403ValGln: 2.403 ± 0.058	3.641ValArg: 3.641 ± 0.069	4.717ValSer: 4.717 ± 0.077	3.606ValThr: 3.606 ± 0.099	5.076ValVal: 5.076 ± 0.092	0.854ValTrp: 0.854 ± 0.031	1.659ValTyr: 1.659 ± 0.046	0.0ValXaa: 0.0 ± 0.0
Trp	0.867TrpAla: 0.867 ± 0.031	0.126TrpCys: 0.126 ± 0.012	0.661TrpAsp: 0.661 ± 0.032	0.484TrpGlu: 0.484 ± 0.027	0.599TrpPhe: 0.599 ± 0.032	0.728TrpGly: 0.728 ± 0.032	0.45TrpHis: 0.45 ± 0.026	0.781TrpIle: 0.781 ± 0.033	0.669TrpLys: 0.669 ± 0.028	1.783TrpLeu: 1.783 ± 0.052	0.441TrpMet: 0.441 ± 0.025	0.451TrpAsn: 0.451 ± 0.024	0.504TrpPro: 0.504 ± 0.025	0.841TrpGln: 0.841 ± 0.034	0.759TrpArg: 0.759 ± 0.033	0.814TrpSer: 0.814 ± 0.033	0.5TrpThr: 0.5 ± 0.027	0.84TrpVal: 0.84 ± 0.033	0.24TrpTrp: 0.24 ± 0.02	0.308TrpTyr: 0.308 ± 0.022	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.405TyrAla: 2.405 ± 0.058	0.293TyrCys: 0.293 ± 0.019	1.474TyrAsp: 1.474 ± 0.048	1.398TyrGlu: 1.398 ± 0.042	1.133TyrPhe: 1.133 ± 0.039	1.966TyrGly: 1.966 ± 0.054	0.734TyrHis: 0.734 ± 0.032	1.493TyrIle: 1.493 ± 0.034	1.167TyrLys: 1.167 ± 0.04	2.816TyrLeu: 2.816 ± 0.06	0.687TyrMet: 0.687 ± 0.03	0.984TyrAsn: 0.984 ± 0.038	1.146TyrPro: 1.146 ± 0.038	1.212TyrGln: 1.212 ± 0.041	1.684TyrArg: 1.684 ± 0.049	1.666TyrSer: 1.666 ± 0.044	1.194TyrThr: 1.194 ± 0.047	1.483TyrVal: 1.483 ± 0.041	0.348TyrTrp: 0.348 ± 0.024	0.767TyrTyr: 0.767 ± 0.03	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 2630 proteins (848503 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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