Woodlouse hunter spider associated circular virus 1
Average proteome isoelectric point is 7.58
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A346BPE4|A0A346BPE4_9VIRU Putative capsid protein OS=Woodlouse hunter spider associated circular virus 1 OX=2293310 PE=4 SV=1
MM1 pKa = 7.18 SRR3 pKa = 11.84 SRR5 pKa = 11.84 SYY7 pKa = 11.3 CFTWNNYY14 pKa = 7.59 PVNHH18 pKa = 6.27 QDD20 pKa = 3.15 VLDD23 pKa = 4.05 RR24 pKa = 11.84 LGYY27 pKa = 10.19 RR28 pKa = 11.84 YY29 pKa = 10.12 VCYY32 pKa = 9.99 GYY34 pKa = 10.5 EE35 pKa = 3.88 WAPTTRR41 pKa = 11.84 TPHH44 pKa = 5.44 LQGFIYY50 pKa = 10.29 FRR52 pKa = 11.84 DD53 pKa = 3.89 AKK55 pKa = 9.65 TMRR58 pKa = 11.84 TVVRR62 pKa = 11.84 GLPGVHH68 pKa = 6.29 IEE70 pKa = 4.33 SARR73 pKa = 11.84 GTPAQCIEE81 pKa = 4.13 YY82 pKa = 9.77 CRR84 pKa = 11.84 KK85 pKa = 10.26 DD86 pKa = 3.29 GEE88 pKa = 4.34 FIEE91 pKa = 5.52 FGEE94 pKa = 4.72 APQTPQEE101 pKa = 3.98 IGEE104 pKa = 4.28 AEE106 pKa = 4.1 KK107 pKa = 10.97 DD108 pKa = 3.4 RR109 pKa = 11.84 YY110 pKa = 8.08 LTAWDD115 pKa = 3.97 LAKK118 pKa = 10.58 AGQIDD123 pKa = 4.55 EE124 pKa = 4.76 IDD126 pKa = 3.63 PEE128 pKa = 4.21 LRR130 pKa = 11.84 LRR132 pKa = 11.84 HH133 pKa = 5.48 YY134 pKa = 9.62 STLKK138 pKa = 10.84 SIAKK142 pKa = 9.74 DD143 pKa = 3.54 YY144 pKa = 9.92 MVRR147 pKa = 11.84 PPQLDD152 pKa = 3.84 GVCGVWFWGPAGSGKK167 pKa = 7.33 TTTANRR173 pKa = 11.84 LYY175 pKa = 10.06 PNAYY179 pKa = 10.13 LKK181 pKa = 10.3 PLNKK185 pKa = 9.14 WWDD188 pKa = 3.87 GYY190 pKa = 10.53 QGEE193 pKa = 4.93 DD194 pKa = 4.36 VVICDD199 pKa = 4.68 DD200 pKa = 3.25 MSIFHH205 pKa = 7.21 RR206 pKa = 11.84 SLSDD210 pKa = 4.23 LLKK213 pKa = 10.39 HH214 pKa = 5.39 WTDD217 pKa = 4.19 FLPFIAEE224 pKa = 4.1 VKK226 pKa = 10.3 RR227 pKa = 11.84 EE228 pKa = 4.07 SVYY231 pKa = 10.3 IRR233 pKa = 11.84 PSKK236 pKa = 10.65 FIVTSQYY243 pKa = 10.95 SIEE246 pKa = 4.6 EE247 pKa = 3.53 IWAGDD252 pKa = 3.9 EE253 pKa = 4.14 KK254 pKa = 11.18 SLEE257 pKa = 3.8 AMRR260 pKa = 11.84 RR261 pKa = 11.84 RR262 pKa = 11.84 FTVVEE267 pKa = 4.41 KK268 pKa = 11.08 VAGQDD273 pKa = 3.64 IIMLL277 pKa = 4.4
Molecular weight: 32.15 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.91
IPC2_protein 5.982
IPC_protein 6.059
Toseland 6.3
ProMoST 6.415
Dawson 6.351
Bjellqvist 6.326
Wikipedia 6.364
Rodwell 6.338
Grimsley 6.415
Solomon 6.351
Lehninger 6.351
Nozaki 6.605
DTASelect 6.795
Thurlkill 6.81
EMBOSS 6.781
Sillero 6.722
Patrickios 3.961
IPC_peptide 6.364
IPC2_peptide 6.634
IPC2.peptide.svr19 6.628
Protein with the highest isoelectric point:
>tr|A0A346BPE4|A0A346BPE4_9VIRU Putative capsid protein OS=Woodlouse hunter spider associated circular virus 1 OX=2293310 PE=4 SV=1
MM1 pKa = 7.35 PRR3 pKa = 11.84 RR4 pKa = 11.84 RR5 pKa = 11.84 RR6 pKa = 11.84 TAKK9 pKa = 8.67 RR10 pKa = 11.84 RR11 pKa = 11.84 KK12 pKa = 8.64 RR13 pKa = 11.84 RR14 pKa = 11.84 TVKK17 pKa = 10.11 RR18 pKa = 11.84 RR19 pKa = 11.84 SKK21 pKa = 10.21 KK22 pKa = 9.66 GRR24 pKa = 11.84 RR25 pKa = 11.84 LGKK28 pKa = 9.57 RR29 pKa = 11.84 QMDD32 pKa = 4.31 IYY34 pKa = 10.12 PSTGDD39 pKa = 3.43 YY40 pKa = 11.04 VYY42 pKa = 11.68 GDD44 pKa = 3.73 MHH46 pKa = 8.46 RR47 pKa = 11.84 MAKK50 pKa = 9.98 KK51 pKa = 10.06 IKK53 pKa = 8.41 TSLGGSSSYY62 pKa = 9.63 TASRR66 pKa = 11.84 KK67 pKa = 10.07 DD68 pKa = 3.34 DD69 pKa = 4.43 DD70 pKa = 3.92 QWSMTQEE77 pKa = 4.21 DD78 pKa = 4.79 GIKK81 pKa = 10.48 YY82 pKa = 8.23 KK83 pKa = 10.32 TINITYY89 pKa = 8.94 KK90 pKa = 9.72 KK91 pKa = 10.35 SKK93 pKa = 9.84 IGRR96 pKa = 11.84 LTQRR100 pKa = 11.84 LTSPGNLYY108 pKa = 10.69 DD109 pKa = 3.75 YY110 pKa = 9.68 ATGGGASTQGRR121 pKa = 11.84 QAVTDD126 pKa = 4.21 ASAFNGGDD134 pKa = 3.62 FNTLFQGLNNGVAITAIKK152 pKa = 10.41 ASNQLFVKK160 pKa = 9.47 QVKK163 pKa = 10.29 HH164 pKa = 5.73 EE165 pKa = 4.18 MEE167 pKa = 4.55 FNNAGPTTLEE177 pKa = 3.57 MDD179 pKa = 3.94 VYY181 pKa = 11.16 ILIDD185 pKa = 3.89 KK186 pKa = 8.1 NTGGAISNPLTIWDD200 pKa = 3.75 AALNSEE206 pKa = 4.56 TNLVDD211 pKa = 5.55 AIVEE215 pKa = 4.28 AKK217 pKa = 10.03 NKK219 pKa = 9.32 PWLKK223 pKa = 9.2 PTTLKK228 pKa = 11.04 LFWFYY233 pKa = 10.69 PKK235 pKa = 10.33 SIPKK239 pKa = 9.96 SIQTSRR245 pKa = 11.84 IRR247 pKa = 11.84 DD248 pKa = 3.76 SNSTSHH254 pKa = 7.33 TIPPRR259 pKa = 3.89
Molecular weight: 29.13 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.247
IPC2_protein 9.721
IPC_protein 10.014
Toseland 10.57
ProMoST 10.204
Dawson 10.687
Bjellqvist 10.321
Wikipedia 10.833
Rodwell 11.169
Grimsley 10.73
Solomon 10.73
Lehninger 10.701
Nozaki 10.526
DTASelect 10.321
Thurlkill 10.57
EMBOSS 10.95
Sillero 10.599
Patrickios 10.877
IPC_peptide 10.73
IPC2_peptide 8.858
IPC2.peptide.svr19 8.749
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
0
2
536
259
277
268.0
30.64
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.343 ± 0.149
1.119 ± 0.758
6.157 ± 0.247
4.664 ± 1.59
3.358 ± 0.444
7.276 ± 0.302
1.679 ± 0.353
6.343 ± 0.149
7.276 ± 1.609
6.53 ± 0.239
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.425 ± 0.188
3.918 ± 1.269
4.851 ± 0.409
3.731 ± 0.088
7.836 ± 0.184
6.716 ± 0.942
7.649 ± 1.356
4.851 ± 0.932
2.425 ± 0.597
4.851 ± 0.67
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here