Capybara microvirus Cap3_SP_315
Average proteome isoelectric point is 6.81
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4P8W534|A0A4P8W534_9VIRU Major capsid protein OS=Capybara microvirus Cap3_SP_315 OX=2585425 PE=4 SV=1
MM1 pKa = 7.41 SFKK4 pKa = 10.89 KK5 pKa = 10.75 LIDD8 pKa = 3.7 FQLPKK13 pKa = 10.43 VDD15 pKa = 4.57 DD16 pKa = 4.12 PSILVHH22 pKa = 6.7 EE23 pKa = 5.65 DD24 pKa = 3.01 IEE26 pKa = 4.59 GTNFSRR32 pKa = 11.84 LVEE35 pKa = 4.43 KK36 pKa = 9.97 PLSSIHH42 pKa = 6.91 VDD44 pKa = 3.36 LPDD47 pKa = 3.58 FRR49 pKa = 11.84 LVSLSEE55 pKa = 3.78 ILSRR59 pKa = 11.84 GEE61 pKa = 4.17 DD62 pKa = 3.59 LQKK65 pKa = 10.8 VDD67 pKa = 3.51 TKK69 pKa = 11.07 LFEE72 pKa = 5.01 SNLPDD77 pKa = 3.79 FDD79 pKa = 4.93 NDD81 pKa = 2.86 IEE83 pKa = 4.67 QIVNTYY89 pKa = 9.54 EE90 pKa = 3.87 SS91 pKa = 3.49
Molecular weight: 10.46 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.519
IPC2_protein 4.329
IPC_protein 4.24
Toseland 4.062
ProMoST 4.38
Dawson 4.215
Bjellqvist 4.368
Wikipedia 4.126
Rodwell 4.075
Grimsley 3.973
Solomon 4.215
Lehninger 4.164
Nozaki 4.329
DTASelect 4.546
Thurlkill 4.088
EMBOSS 4.139
Sillero 4.368
Patrickios 4.113
IPC_peptide 4.215
IPC2_peptide 4.342
IPC2.peptide.svr19 4.308
Protein with the highest isoelectric point:
>tr|A0A4P8W8A8|A0A4P8W8A8_9VIRU Uncharacterized protein OS=Capybara microvirus Cap3_SP_315 OX=2585425 PE=4 SV=1
MM1 pKa = 7.59 CLSPIWIKK9 pKa = 11.12 NPTRR13 pKa = 11.84 DD14 pKa = 3.52 YY15 pKa = 11.37 RR16 pKa = 11.84 PIDD19 pKa = 3.53 KK20 pKa = 10.41 VRR22 pKa = 11.84 LQIPCGKK29 pKa = 10.03 CPQCQQQKK37 pKa = 10.21 AKK39 pKa = 10.47 DD40 pKa = 3.57 YY41 pKa = 8.92 EE42 pKa = 4.11 FRR44 pKa = 11.84 MLSDD48 pKa = 3.97 MYY50 pKa = 10.9 RR51 pKa = 11.84 KK52 pKa = 9.58 GRR54 pKa = 11.84 SFFFYY59 pKa = 10.34 TLTYY63 pKa = 10.59 NNQSLPYY70 pKa = 9.67 RR71 pKa = 11.84 EE72 pKa = 5.23 FYY74 pKa = 10.53 TLDD77 pKa = 3.69 GEE79 pKa = 4.7 IKK81 pKa = 10.29 HH82 pKa = 5.77 YY83 pKa = 9.33 MCFVKK88 pKa = 10.61 DD89 pKa = 3.83 HH90 pKa = 6.95 IKK92 pKa = 8.46 THH94 pKa = 6.08 INNIRR99 pKa = 11.84 KK100 pKa = 8.96 YY101 pKa = 8.24 LHH103 pKa = 6.03 KK104 pKa = 10.14 HH105 pKa = 5.6 FSFKK109 pKa = 10.66 GCSFFCVPEE118 pKa = 3.96 YY119 pKa = 11.36 GDD121 pKa = 3.46 LKK123 pKa = 11.07 KK124 pKa = 10.27 RR125 pKa = 11.84 VHH127 pKa = 4.12 WHH129 pKa = 6.01 VYY131 pKa = 9.27 FSTPVKK137 pKa = 10.3 FDD139 pKa = 3.06 AKK141 pKa = 10.59 KK142 pKa = 10.2 FLEE145 pKa = 4.11 IVNRR149 pKa = 11.84 FWNYY153 pKa = 9.19 GLVFPEE159 pKa = 3.55 HH160 pKa = 6.71 HH161 pKa = 6.55 YY162 pKa = 10.91 SYY164 pKa = 9.84 KK165 pKa = 6.95 TTRR168 pKa = 11.84 NRR170 pKa = 11.84 LCRR173 pKa = 11.84 GIEE176 pKa = 3.76 IKK178 pKa = 10.93 NPEE181 pKa = 3.89 TSSYY185 pKa = 10.76 YY186 pKa = 8.56 IAHH189 pKa = 6.32 YY190 pKa = 9.41 VTDD193 pKa = 4.16 FSIIRR198 pKa = 11.84 DD199 pKa = 3.4 IPHH202 pKa = 5.67 VDD204 pKa = 2.86 KK205 pKa = 11.33 YY206 pKa = 11.81 LNGLLLDD213 pKa = 4.73 NDD215 pKa = 4.24 TKK217 pKa = 11.29 SYY219 pKa = 8.34 TLRR222 pKa = 11.84 PFVFMSKK229 pKa = 10.32 SLGDD233 pKa = 3.64 NEE235 pKa = 4.73 EE236 pKa = 4.06 LRR238 pKa = 11.84 KK239 pKa = 10.71 DD240 pKa = 3.4 PDD242 pKa = 3.39 KK243 pKa = 11.19 LISYY247 pKa = 7.55 FKK249 pKa = 10.52 NGYY252 pKa = 6.82 ITPFGKK258 pKa = 10.44 KK259 pKa = 9.67 LRR261 pKa = 11.84 VPLFIIRR268 pKa = 11.84 KK269 pKa = 5.85 TLFDD273 pKa = 4.98 IEE275 pKa = 4.44 YY276 pKa = 9.66 KK277 pKa = 11.02 DD278 pKa = 4.43 NGRR281 pKa = 11.84 KK282 pKa = 9.7 DD283 pKa = 3.75 KK284 pKa = 11.26 NNKK287 pKa = 8.91 PSLDD291 pKa = 3.56 VIYY294 pKa = 10.46 KK295 pKa = 10.44 LNSLGFKK302 pKa = 10.05 FYY304 pKa = 11.08 HH305 pKa = 6.08 EE306 pKa = 4.67 NFGNMIKK313 pKa = 10.3 QKK315 pKa = 10.72 AEE317 pKa = 3.77 KK318 pKa = 9.21 LAHH321 pKa = 6.82 GFRR324 pKa = 11.84 CMKK327 pKa = 10.32 ALRR330 pKa = 11.84 PQFFNIIEE338 pKa = 4.26 KK339 pKa = 10.66 KK340 pKa = 10.23 FDD342 pKa = 3.37 GSFEE346 pKa = 4.62 DD347 pKa = 3.95 KK348 pKa = 11.13 LCINFFNDD356 pKa = 2.96 KK357 pKa = 10.94 QIFTKK362 pKa = 10.54 FALYY366 pKa = 7.68 DD367 pKa = 3.31 TCFRR371 pKa = 11.84 DD372 pKa = 3.75 VKK374 pKa = 10.64 SQNFMFGVPQSVKK387 pKa = 10.68 DD388 pKa = 3.67 LFRR391 pKa = 11.84 ISPDD395 pKa = 2.5 IYY397 pKa = 10.62 LQRR400 pKa = 11.84 KK401 pKa = 7.6 LQYY404 pKa = 9.9 KK405 pKa = 9.01 PLIGKK410 pKa = 8.92 SSPKK414 pKa = 9.94 YY415 pKa = 9.67 DD416 pKa = 3.5 VKK418 pKa = 10.82 EE419 pKa = 4.04 TFSNYY424 pKa = 9.82 RR425 pKa = 11.84 EE426 pKa = 4.0 FVFWDD431 pKa = 3.42 YY432 pKa = 11.43 CIDD435 pKa = 3.84 SFNSCSARR443 pKa = 11.84 TYY445 pKa = 10.58 SRR447 pKa = 11.84 SVFNLLEE454 pKa = 4.15 FDD456 pKa = 4.34 KK457 pKa = 10.97 KK458 pKa = 11.17 SQILDD463 pKa = 3.51 LNYY466 pKa = 10.07 VFF468 pKa = 5.92
Molecular weight: 56.29 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.808
IPC2_protein 8.858
IPC_protein 8.712
Toseland 9.355
ProMoST 9.194
Dawson 9.677
Bjellqvist 9.443
Wikipedia 9.853
Rodwell 9.926
Grimsley 9.75
Solomon 9.692
Lehninger 9.648
Nozaki 9.531
DTASelect 9.385
Thurlkill 9.516
EMBOSS 9.809
Sillero 9.633
Patrickios 4.546
IPC_peptide 9.677
IPC2_peptide 8.141
IPC2.peptide.svr19 7.824
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
6
0
6
1784
68
659
297.3
34.17
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
4.372 ± 2.094
1.794 ± 0.463
6.67 ± 0.728
4.092 ± 0.528
6.502 ± 1.54
4.372 ± 0.723
2.074 ± 0.542
6.839 ± 0.546
7.287 ± 1.517
10.258 ± 1.047
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.289 ± 0.262
7.231 ± 0.959
3.98 ± 0.394
4.204 ± 0.701
4.092 ± 0.56
8.352 ± 0.65
6.11 ± 1.242
4.652 ± 0.327
0.841 ± 0.22
4.989 ± 0.954
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here