Capybara microvirus Cap1_SP_158
Average proteome isoelectric point is 5.94
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4P8W7Y9|A0A4P8W7Y9_9VIRU Uncharacterized protein OS=Capybara microvirus Cap1_SP_158 OX=2585394 PE=4 SV=1
MM1 pKa = 7.48 NKK3 pKa = 9.85 NSMFQEE9 pKa = 4.72 HH10 pKa = 7.16 PSSFQLFMLGDD21 pKa = 3.17 ICEE24 pKa = 4.54 NMNAMCTEE32 pKa = 4.3 FDD34 pKa = 4.47 SVDD37 pKa = 3.61 LLLPMDD43 pKa = 3.91 EE44 pKa = 4.38 TGEE47 pKa = 4.16 RR48 pKa = 11.84 CCIPLEE54 pKa = 4.13 SLGTVGEE61 pKa = 4.65 FYY63 pKa = 10.66 RR64 pKa = 11.84 KK65 pKa = 9.37 EE66 pKa = 4.07 SIVYY70 pKa = 9.99 EE71 pKa = 4.24 EE72 pKa = 4.09 EE73 pKa = 4.1 HH74 pKa = 6.52 LL75 pKa = 4.2
Molecular weight: 8.67 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.949
IPC2_protein 4.228
IPC_protein 4.088
Toseland 3.935
ProMoST 4.19
Dawson 4.024
Bjellqvist 4.177
Wikipedia 3.884
Rodwell 3.923
Grimsley 3.859
Solomon 4.012
Lehninger 3.961
Nozaki 4.151
DTASelect 4.228
Thurlkill 3.961
EMBOSS 3.91
Sillero 4.19
Patrickios 0.846
IPC_peptide 4.024
IPC2_peptide 4.19
IPC2.peptide.svr19 4.084
Protein with the highest isoelectric point:
>tr|A0A4P8W473|A0A4P8W473_9VIRU Major capsid protein OS=Capybara microvirus Cap1_SP_158 OX=2585394 PE=3 SV=1
MM1 pKa = 7.66 SSNNQAWYY9 pKa = 8.25 TWSPPKK15 pKa = 8.91 NTKK18 pKa = 8.59 YY19 pKa = 8.29 WHH21 pKa = 6.87 GYY23 pKa = 8.71 LPIGIAIRR31 pKa = 11.84 DD32 pKa = 3.84 NFNTANAIRR41 pKa = 11.84 RR42 pKa = 11.84 AANNFNTDD50 pKa = 3.12 TTSDD54 pKa = 3.28 TKK56 pKa = 10.64 TLQKK60 pKa = 10.69 SLQSLIKK67 pKa = 10.05 GQTSAKK73 pKa = 6.89 TTTNNNINNGYY84 pKa = 9.67 VDD86 pKa = 4.01 TGSSSLDD93 pKa = 3.22 SSLQTLAEE101 pKa = 4.52 HH102 pKa = 6.02 NTNASIEE109 pKa = 4.17 INRR112 pKa = 11.84 MNNAFNASEE121 pKa = 4.0 AQKK124 pKa = 10.87 NRR126 pKa = 11.84 DD127 pKa = 3.21 WQEE130 pKa = 4.05 SMSNTAHH137 pKa = 5.31 QRR139 pKa = 11.84 EE140 pKa = 4.37 VEE142 pKa = 4.07 DD143 pKa = 4.69 LKK145 pKa = 11.41 AAGLNPVLSAGGQGAATGSGSAASSSGNANVDD177 pKa = 3.63 SAVNALASIQTAQISAAAMVSAAAIAAQASMYY209 pKa = 10.87 GSDD212 pKa = 3.52 NALSGTKK219 pKa = 10.33 YY220 pKa = 9.86 STDD223 pKa = 2.75 LTYY226 pKa = 11.36 YY227 pKa = 8.85 KK228 pKa = 10.49 TNSPWNLINRR238 pKa = 11.84 LLPNSDD244 pKa = 3.17 TSAKK248 pKa = 10.43 DD249 pKa = 2.97 ILRR252 pKa = 11.84 SIIGKK257 pKa = 9.18 NEE259 pKa = 3.78 VIFRR263 pKa = 11.84 RR264 pKa = 3.94
Molecular weight: 28.2 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.532
IPC2_protein 8.492
IPC_protein 8.521
Toseland 9.077
ProMoST 9.063
Dawson 9.355
Bjellqvist 9.048
Wikipedia 9.56
Rodwell 9.531
Grimsley 9.458
Solomon 9.472
Lehninger 9.428
Nozaki 8.99
DTASelect 9.063
Thurlkill 9.165
EMBOSS 9.487
Sillero 9.268
Patrickios 4.673
IPC_peptide 9.458
IPC2_peptide 7.614
IPC2.peptide.svr19 7.608
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
5
0
5
1439
75
563
287.8
32.66
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.505 ± 1.592
1.737 ± 0.618
5.768 ± 0.478
6.185 ± 1.416
4.864 ± 0.955
5.559 ± 0.305
2.293 ± 0.574
5.142 ± 0.414
5.073 ± 1.182
7.714 ± 0.991
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.015 ± 0.409
6.115 ± 1.493
4.1 ± 0.474
4.448 ± 0.325
4.656 ± 0.258
8.826 ± 1.13
6.115 ± 0.638
4.656 ± 0.715
1.251 ± 0.283
5.976 ± 0.989
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here