Salarias fasciatus (Jewelled blenny) (Blennius fasciatus)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Deuterostomia; Chordata; Craniata; Vertebrata; Gnathostomata; Teleostomi; Euteleostomi; Actinopterygii; Actinopteri; Neopterygii; Teleostei; Osteoglossocephalai; Clupeocephala; Euteleosteomorpha; Neoteleostei; Eurypterygia;

Average proteome isoelectric point is 6.55

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 55738 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A672HYA4|A0A672HYA4_SALFA ANK_REP_REGION domain-containing protein OS=Salarias fasciatus OX=181472 GN=ankrd39 PE=4 SV=1
LL1 pKa = 8.29SFDD4 pKa = 3.53VTDD7 pKa = 4.17GKK9 pKa = 10.91DD10 pKa = 3.03RR11 pKa = 11.84IIFVTKK17 pKa = 9.82EE18 pKa = 3.87DD19 pKa = 3.6QEE21 pKa = 4.5APSNAEE27 pKa = 4.22LVADD31 pKa = 5.11DD32 pKa = 5.12PDD34 pKa = 4.84DD35 pKa = 4.49PYY37 pKa = 11.6EE38 pKa = 4.16EE39 pKa = 4.3QGLILPSGDD48 pKa = 3.72MNWNCPCLGGMASGPCGSQFKK69 pKa = 9.21EE70 pKa = 3.99ACSCFHH76 pKa = 6.25YY77 pKa = 10.82SKK79 pKa = 11.27EE80 pKa = 4.09EE81 pKa = 4.02VKK83 pKa = 10.98GSDD86 pKa = 3.11CLEE89 pKa = 3.99NVRR92 pKa = 11.84NLQEE96 pKa = 4.57CMQKK100 pKa = 10.11NPEE103 pKa = 4.71LYY105 pKa = 9.59PQEE108 pKa = 4.08EE109 pKa = 4.66DD110 pKa = 3.51EE111 pKa = 6.13DD112 pKa = 4.2VATQQEE118 pKa = 4.56SDD120 pKa = 3.6SALTSAAPPDD130 pKa = 3.93TSALSPEE137 pKa = 4.8SEE139 pKa = 4.28PSPAPSAVTPDD150 pKa = 3.4SSSATDD156 pKa = 3.64GQTASS161 pKa = 3.59

Molecular weight: 17.17 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A672J143|A0A672J143_SALFA Discs large (Drosophila) homolog-associated protein 2b OS=Salarias fasciatus OX=181472 PE=3 SV=1
SS1 pKa = 7.09SYY3 pKa = 11.57NEE5 pKa = 3.59VRR7 pKa = 11.84GQEE10 pKa = 3.89QHH12 pKa = 7.23LSEE15 pKa = 4.27QKK17 pKa = 10.24NRR19 pKa = 11.84EE20 pKa = 3.99YY21 pKa = 11.05SPKK24 pKa = 9.58TRR26 pKa = 11.84LKK28 pKa = 9.52TRR30 pKa = 11.84LKK32 pKa = 9.71TRR34 pKa = 11.84LKK36 pKa = 9.71TRR38 pKa = 11.84LKK40 pKa = 8.97TRR42 pKa = 11.84LRR44 pKa = 11.84TRR46 pKa = 11.84LKK48 pKa = 9.66TRR50 pKa = 11.84LKK52 pKa = 9.71TRR54 pKa = 11.84LKK56 pKa = 8.97TRR58 pKa = 11.84LRR60 pKa = 11.84TRR62 pKa = 11.84LKK64 pKa = 9.57TRR66 pKa = 11.84LKK68 pKa = 10.74NRR70 pKa = 11.84LKK72 pKa = 10.09TRR74 pKa = 11.84LKK76 pKa = 9.65TRR78 pKa = 11.84LKK80 pKa = 9.62TRR82 pKa = 11.84LKK84 pKa = 10.74NRR86 pKa = 11.84LKK88 pKa = 10.09TRR90 pKa = 11.84LKK92 pKa = 9.65TRR94 pKa = 11.84LKK96 pKa = 9.71TRR98 pKa = 11.84LKK100 pKa = 9.62TRR102 pKa = 11.84LKK104 pKa = 10.74NRR106 pKa = 11.84LKK108 pKa = 10.09TRR110 pKa = 11.84LKK112 pKa = 9.65TRR114 pKa = 11.84LKK116 pKa = 9.71TRR118 pKa = 11.84LKK120 pKa = 9.71TRR122 pKa = 11.84LKK124 pKa = 9.71TRR126 pKa = 11.84LKK128 pKa = 9.62TRR130 pKa = 11.84LKK132 pKa = 10.68NRR134 pKa = 11.84LKK136 pKa = 10.92NRR138 pKa = 11.84LKK140 pKa = 10.06TRR142 pKa = 11.84LKK144 pKa = 9.65TRR146 pKa = 11.84LKK148 pKa = 9.71TRR150 pKa = 11.84LKK152 pKa = 9.62TRR154 pKa = 11.84LKK156 pKa = 10.74NRR158 pKa = 11.84LKK160 pKa = 9.73TRR162 pKa = 11.84LRR164 pKa = 11.84TRR166 pKa = 11.84LKK168 pKa = 9.57TRR170 pKa = 11.84LKK172 pKa = 10.74NRR174 pKa = 11.84LKK176 pKa = 9.73TRR178 pKa = 11.84LRR180 pKa = 11.84TRR182 pKa = 11.84LKK184 pKa = 9.66TRR186 pKa = 11.84LKK188 pKa = 9.71TRR190 pKa = 11.84LKK192 pKa = 9.71TRR194 pKa = 11.84LKK196 pKa = 9.62TRR198 pKa = 11.84LKK200 pKa = 10.74NRR202 pKa = 11.84LKK204 pKa = 9.73TRR206 pKa = 11.84LRR208 pKa = 11.84TRR210 pKa = 11.84LKK212 pKa = 9.57TRR214 pKa = 11.84LKK216 pKa = 10.68NRR218 pKa = 11.84LKK220 pKa = 10.92NRR222 pKa = 11.84LKK224 pKa = 9.6TRR226 pKa = 11.84LRR228 pKa = 11.84TRR230 pKa = 11.84LKK232 pKa = 9.57TRR234 pKa = 11.84LKK236 pKa = 10.74NRR238 pKa = 11.84LKK240 pKa = 9.73TRR242 pKa = 11.84LRR244 pKa = 11.84TRR246 pKa = 11.84LKK248 pKa = 9.66TRR250 pKa = 11.84LKK252 pKa = 9.71TRR254 pKa = 11.84LKK256 pKa = 9.62TRR258 pKa = 11.84LKK260 pKa = 10.74NRR262 pKa = 11.84LKK264 pKa = 9.73TRR266 pKa = 11.84LRR268 pKa = 11.84TRR270 pKa = 11.84LKK272 pKa = 9.57TRR274 pKa = 11.84LKK276 pKa = 10.74NRR278 pKa = 11.84LKK280 pKa = 10.09TRR282 pKa = 11.84LKK284 pKa = 9.65TRR286 pKa = 11.84LKK288 pKa = 9.71TRR290 pKa = 11.84LKK292 pKa = 9.71TRR294 pKa = 11.84LKK296 pKa = 9.71TRR298 pKa = 11.84LKK300 pKa = 9.71TRR302 pKa = 11.84LKK304 pKa = 9.71TRR306 pKa = 11.84LKK308 pKa = 9.71TRR310 pKa = 11.84LKK312 pKa = 9.71TRR314 pKa = 11.84LKK316 pKa = 9.95TRR318 pKa = 11.84LKK320 pKa = 9.62TKK322 pKa = 10.58LKK324 pKa = 9.52TRR326 pKa = 11.84LKK328 pKa = 9.52TRR330 pKa = 11.84LKK332 pKa = 9.71TRR334 pKa = 11.84LKK336 pKa = 9.71TRR338 pKa = 11.84LKK340 pKa = 9.71TRR342 pKa = 11.84LKK344 pKa = 9.71TRR346 pKa = 11.84LKK348 pKa = 9.71TRR350 pKa = 11.84LKK352 pKa = 9.62TRR354 pKa = 11.84LKK356 pKa = 10.74NRR358 pKa = 11.84LKK360 pKa = 9.73TRR362 pKa = 11.84LRR364 pKa = 11.84TRR366 pKa = 11.84LKK368 pKa = 9.57TRR370 pKa = 11.84LKK372 pKa = 10.74NRR374 pKa = 11.84LKK376 pKa = 9.73TRR378 pKa = 11.84LRR380 pKa = 11.84TRR382 pKa = 11.84LKK384 pKa = 9.57TRR386 pKa = 11.84LKK388 pKa = 10.74NRR390 pKa = 11.84LKK392 pKa = 10.09TRR394 pKa = 11.84LKK396 pKa = 9.55TRR398 pKa = 11.84LKK400 pKa = 10.74NRR402 pKa = 11.84LKK404 pKa = 9.73TRR406 pKa = 11.84LRR408 pKa = 11.84TRR410 pKa = 11.84LKK412 pKa = 9.57TRR414 pKa = 11.84LKK416 pKa = 10.74NRR418 pKa = 11.84LKK420 pKa = 9.73TRR422 pKa = 11.84LRR424 pKa = 11.84TRR426 pKa = 11.84LKK428 pKa = 9.57TRR430 pKa = 11.84LKK432 pKa = 10.74NRR434 pKa = 11.84LKK436 pKa = 9.7TRR438 pKa = 11.84LRR440 pKa = 11.84TRR442 pKa = 11.84IKK444 pKa = 9.74TRR446 pKa = 11.84LKK448 pKa = 9.5TRR450 pKa = 11.84LKK452 pKa = 10.74NRR454 pKa = 11.84LKK456 pKa = 10.09TRR458 pKa = 11.84LKK460 pKa = 9.65TRR462 pKa = 11.84LKK464 pKa = 9.71TRR466 pKa = 11.84LKK468 pKa = 9.71TRR470 pKa = 11.84LKK472 pKa = 9.62TRR474 pKa = 11.84LKK476 pKa = 10.74NRR478 pKa = 11.84LKK480 pKa = 10.09TRR482 pKa = 11.84LKK484 pKa = 9.65TRR486 pKa = 11.84LKK488 pKa = 9.28TRR490 pKa = 11.84LRR492 pKa = 11.84TTLKK496 pKa = 9.59TRR498 pKa = 11.84LKK500 pKa = 10.46NRR502 pKa = 11.84LKK504 pKa = 9.62TRR506 pKa = 11.84VRR508 pKa = 11.84TRR510 pKa = 11.84LKK512 pKa = 9.79TRR514 pKa = 11.84LKK516 pKa = 9.71TRR518 pKa = 11.84LKK520 pKa = 9.71TRR522 pKa = 11.84LKK524 pKa = 9.71TRR526 pKa = 11.84LKK528 pKa = 8.97TRR530 pKa = 11.84LRR532 pKa = 11.84TRR534 pKa = 11.84LKK536 pKa = 9.57TRR538 pKa = 11.84LKK540 pKa = 10.74NRR542 pKa = 11.84LKK544 pKa = 10.09TRR546 pKa = 11.84LKK548 pKa = 9.65TRR550 pKa = 11.84LKK552 pKa = 9.71TRR554 pKa = 11.84LKK556 pKa = 8.97TRR558 pKa = 11.84LRR560 pKa = 11.84TRR562 pKa = 11.84LKK564 pKa = 10.71NRR566 pKa = 11.84LKK568 pKa = 10.09TRR570 pKa = 11.84LKK572 pKa = 9.65TRR574 pKa = 11.84LKK576 pKa = 9.38TRR578 pKa = 11.84LKK580 pKa = 10.87ARR582 pKa = 11.84LKK584 pKa = 9.77VAFISPHH591 pKa = 5.36VEE593 pKa = 3.7EE594 pKa = 4.6TASSRR599 pKa = 11.84RR600 pKa = 11.84KK601 pKa = 10.39EE602 pKa = 3.69EE603 pKa = 4.01DD604 pKa = 2.77

Molecular weight: 75.63 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

24694

31044

55738

33321467

17

7540

597.8

66.81

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.778 ± 0.009

2.313 ± 0.007

5.267 ± 0.008

6.722 ± 0.012

3.903 ± 0.007

6.412 ± 0.01

2.642 ± 0.005

4.418 ± 0.007

5.479 ± 0.01

9.784 ± 0.013

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.323 ± 0.004

3.738 ± 0.006

5.566 ± 0.012

4.58 ± 0.008

5.788 ± 0.008

8.345 ± 0.013

5.386 ± 0.009

6.524 ± 0.008

1.205 ± 0.003

2.826 ± 0.005
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski