Acaryochloris marina (strain MBIC 11017)
Average proteome isoelectric point is 6.34
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 8172 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|B0C6L6|B0C6L6_ACAM1 TPR domain protein OS=Acaryochloris marina (strain MBIC 11017) OX=329726 GN=AM1_1406 PE=4 SV=1
MM1 pKa = 7.8 ILTLPEE7 pKa = 3.91 NEE9 pKa = 4.14 EE10 pKa = 4.02 RR11 pKa = 11.84 DD12 pKa = 3.55 LEE14 pKa = 4.33 LQVRR18 pKa = 11.84 HH19 pKa = 5.29 QTPDD23 pKa = 3.32 DD24 pKa = 4.25 AQPSAQLYY32 pKa = 10.0 EE33 pKa = 4.26 EE34 pKa = 4.16 THH36 pKa = 6.71 DD37 pKa = 3.68 GQRR40 pKa = 11.84 CHH42 pKa = 6.68 GVDD45 pKa = 4.16 PDD47 pKa = 3.94 QDD49 pKa = 4.18 CDD51 pKa = 3.4 WVYY54 pKa = 11.27 TDD56 pKa = 4.26 SDD58 pKa = 3.85 YY59 pKa = 11.82 DD60 pKa = 3.63 EE61 pKa = 4.71 FF62 pKa = 6.52
Molecular weight: 7.31 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.722
IPC2_protein 3.808
IPC_protein 3.757
Toseland 3.554
ProMoST 3.935
Dawson 3.757
Bjellqvist 3.923
Wikipedia 3.706
Rodwell 3.592
Grimsley 3.465
Solomon 3.745
Lehninger 3.694
Nozaki 3.884
DTASelect 4.113
Thurlkill 3.617
EMBOSS 3.719
Sillero 3.884
Patrickios 0.846
IPC_peptide 3.732
IPC2_peptide 3.859
IPC2.peptide.svr19 3.79
Protein with the highest isoelectric point:
>tr|A8ZLM9|A8ZLM9_ACAM1 Uncharacterized protein OS=Acaryochloris marina (strain MBIC 11017) OX=329726 GN=AM1_B0338 PE=4 SV=1
MM1 pKa = 7.54 HH2 pKa = 7.27 SPYY5 pKa = 11.02 SLNQLGALVRR15 pKa = 11.84 PFLLSLQAPLQQFQLAVEE33 pKa = 4.35 LVEE36 pKa = 4.62 KK37 pKa = 10.7 ARR39 pKa = 11.84 SSNLLAITCRR49 pKa = 11.84 QKK51 pKa = 10.66 IFQTNINPNRR61 pKa = 11.84 MPMRR65 pKa = 11.84 WDD67 pKa = 3.15 FRR69 pKa = 11.84 DD70 pKa = 3.31 RR71 pKa = 11.84 DD72 pKa = 3.57 ISLDD76 pKa = 3.69 TQHH79 pKa = 7.16 DD80 pKa = 4.1 VPARR84 pKa = 11.84 MLADD88 pKa = 3.5 NPYY91 pKa = 10.39 CLDD94 pKa = 3.49 IKK96 pKa = 10.68 IIGYY100 pKa = 10.47 GSVQGNRR107 pKa = 11.84 NPTNLGQLNLITLNWAGLEE126 pKa = 3.93 LRR128 pKa = 11.84 EE129 pKa = 4.49 QEE131 pKa = 4.16 GTVLSKK137 pKa = 10.83 LLKK140 pKa = 9.23 AWKK143 pKa = 8.37 TVTSLPHH150 pKa = 5.17 GLKK153 pKa = 10.23 RR154 pKa = 11.84 RR155 pKa = 11.84 INPAQNILQYY165 pKa = 11.32 LRR167 pKa = 11.84 MHH169 pKa = 7.3 ILQVWQDD176 pKa = 3.95 FLSLSQVFLLRR187 pKa = 11.84 VIARR191 pKa = 11.84 IGSICRR197 pKa = 11.84 NDD199 pKa = 3.51 VLRR202 pKa = 11.84 LQRR205 pKa = 11.84 TSVYY209 pKa = 10.5 LALTATDD216 pKa = 5.14 PIFSLSQRR224 pKa = 11.84 IVVHH228 pKa = 6.58 RR229 pKa = 11.84 ATSLKK234 pKa = 9.85 PRR236 pKa = 11.84 QHH238 pKa = 6.61 PCLLLKK244 pKa = 10.88 ARR246 pKa = 11.84 VNPVAVRR253 pKa = 11.84 HH254 pKa = 5.93 GEE256 pKa = 3.96 HH257 pKa = 6.02 VLIIAFSRR265 pKa = 11.84 EE266 pKa = 4.33 VLCSPP271 pKa = 5.2
Molecular weight: 30.93 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.348
IPC2_protein 9.531
IPC_protein 10.248
Toseland 10.511
ProMoST 10.204
Dawson 10.628
Bjellqvist 10.35
Wikipedia 10.833
Rodwell 10.833
Grimsley 10.687
Solomon 10.73
Lehninger 10.701
Nozaki 10.526
DTASelect 10.335
Thurlkill 10.526
EMBOSS 10.921
Sillero 10.57
Patrickios 10.526
IPC_peptide 10.73
IPC2_peptide 9.516
IPC2.peptide.svr19 8.549
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
8172
0
8172
2253963
29
3597
275.8
30.69
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.318 ± 0.03
1.102 ± 0.011
5.281 ± 0.025
5.782 ± 0.029
3.893 ± 0.021
6.767 ± 0.038
2.218 ± 0.017
6.05 ± 0.024
4.283 ± 0.028
11.033 ± 0.037
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.01 ± 0.013
3.755 ± 0.026
5.065 ± 0.027
6.055 ± 0.035
5.156 ± 0.026
6.612 ± 0.028
5.803 ± 0.032
6.451 ± 0.022
1.524 ± 0.016
2.842 ± 0.017
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here