Amino acid dipepetide frequency for Clostridium sp. CAG:7

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	8.912AlaAla: 8.912 ± 0.151	1.232AlaCys: 1.232 ± 0.045	4.762AlaAsp: 4.762 ± 0.075	5.59AlaGlu: 5.59 ± 0.099	3.236AlaPhe: 3.236 ± 0.062	7.152AlaGly: 7.152 ± 0.098	1.24AlaHis: 1.24 ± 0.034	4.928AlaIle: 4.928 ± 0.096	5.035AlaLys: 5.035 ± 0.075	7.382AlaLeu: 7.382 ± 0.105	2.738AlaMet: 2.738 ± 0.058	2.374AlaAsn: 2.374 ± 0.054	2.269AlaPro: 2.269 ± 0.056	2.504AlaGln: 2.504 ± 0.052	2.913AlaArg: 2.913 ± 0.057	4.301AlaSer: 4.301 ± 0.079	3.321AlaThr: 3.321 ± 0.066	6.948AlaVal: 6.948 ± 0.116	0.683AlaTrp: 0.683 ± 0.027	2.783AlaTyr: 2.783 ± 0.06	0.004AlaXaa: 0.004 ± 0.002
Cys	1.086CysAla: 1.086 ± 0.034	0.368CysCys: 0.368 ± 0.024	0.831CysAsp: 0.831 ± 0.035	0.887CysGlu: 0.887 ± 0.029	0.674CysPhe: 0.674 ± 0.027	1.496CysGly: 1.496 ± 0.043	0.319CysHis: 0.319 ± 0.018	1.088CysIle: 1.088 ± 0.033	0.726CysLys: 0.726 ± 0.031	1.333CysLeu: 1.333 ± 0.041	0.455CysMet: 0.455 ± 0.023	0.546CysAsn: 0.546 ± 0.027	0.663CysPro: 0.663 ± 0.031	0.459CysGln: 0.459 ± 0.019	0.726CysArg: 0.726 ± 0.029	1.017CysSer: 1.017 ± 0.036	0.757CysThr: 0.757 ± 0.03	1.032CysVal: 1.032 ± 0.033	0.108CysTrp: 0.108 ± 0.01	0.563CysTyr: 0.563 ± 0.028	0.0CysXaa: 0.0 ± 0.0
Asp	4.106AspAla: 4.106 ± 0.08	0.826AspCys: 0.826 ± 0.034	2.832AspAsp: 2.832 ± 0.071	4.394AspGlu: 4.394 ± 0.081	2.466AspPhe: 2.466 ± 0.05	4.539AspGly: 4.539 ± 0.087	1.19AspHis: 1.19 ± 0.039	3.989AspIle: 3.989 ± 0.065	3.264AspLys: 3.264 ± 0.059	4.966AspLeu: 4.966 ± 0.073	1.923AspMet: 1.923 ± 0.05	1.92AspAsn: 1.92 ± 0.047	2.194AspPro: 2.194 ± 0.059	1.8AspGln: 1.8 ± 0.045	2.403AspArg: 2.403 ± 0.055	2.697AspSer: 2.697 ± 0.062	3.057AspThr: 3.057 ± 0.069	3.719AspVal: 3.719 ± 0.077	0.59AspTrp: 0.59 ± 0.026	2.625AspTyr: 2.625 ± 0.053	0.001AspXaa: 0.001 ± 0.001
Glu	5.963GluAla: 5.963 ± 0.093	0.726GluCys: 0.726 ± 0.027	4.436GluAsp: 4.436 ± 0.097	7.484GluGlu: 7.484 ± 0.145	2.436GluPhe: 2.436 ± 0.053	4.851GluGly: 4.851 ± 0.087	1.411GluHis: 1.411 ± 0.045	4.998GluIle: 4.998 ± 0.097	6.991GluLys: 6.991 ± 0.104	6.387GluLeu: 6.387 ± 0.1	2.412GluMet: 2.412 ± 0.048	4.114GluAsn: 4.114 ± 0.079	2.069GluPro: 2.069 ± 0.046	2.828GluGln: 2.828 ± 0.059	3.234GluArg: 3.234 ± 0.058	3.069GluSer: 3.069 ± 0.07	4.022GluThr: 4.022 ± 0.097	4.196GluVal: 4.196 ± 0.074	0.612GluTrp: 0.612 ± 0.023	2.79GluTyr: 2.79 ± 0.069	0.001GluXaa: 0.001 ± 0.001
Phe	3.123PheAla: 3.123 ± 0.063	0.717PheCys: 0.717 ± 0.028	2.405PheAsp: 2.405 ± 0.056	2.416PheGlu: 2.416 ± 0.048	1.735PhePhe: 1.735 ± 0.054	3.174PheGly: 3.174 ± 0.072	0.849PheHis: 0.849 ± 0.029	2.699PheIle: 2.699 ± 0.06	2.238PheLys: 2.238 ± 0.049	4.047PheLeu: 4.047 ± 0.1	1.348PheMet: 1.348 ± 0.049	1.479PheAsn: 1.479 ± 0.048	1.482PhePro: 1.482 ± 0.045	1.201PheGln: 1.201 ± 0.038	1.539PheArg: 1.539 ± 0.044	2.768PheSer: 2.768 ± 0.057	2.394PheThr: 2.394 ± 0.051	2.674PheVal: 2.674 ± 0.051	0.463PheTrp: 0.463 ± 0.024	1.689PheTyr: 1.689 ± 0.042	0.0PheXaa: 0.0 ± 0.0
Gly	5.735GlyAla: 5.735 ± 0.09	1.429GlyCys: 1.429 ± 0.041	3.605GlyAsp: 3.605 ± 0.071	4.871GlyGlu: 4.871 ± 0.08	3.248GlyPhe: 3.248 ± 0.058	5.221GlyGly: 5.221 ± 0.092	1.286GlyHis: 1.286 ± 0.036	6.411GlyIle: 6.411 ± 0.09	5.834GlyLys: 5.834 ± 0.087	6.506GlyLeu: 6.506 ± 0.087	2.74GlyMet: 2.74 ± 0.045	3.234GlyAsn: 3.234 ± 0.064	1.644GlyPro: 1.644 ± 0.05	2.251GlyGln: 2.251 ± 0.052	3.214GlyArg: 3.214 ± 0.062	4.497GlySer: 4.497 ± 0.083	4.549GlyThr: 4.549 ± 0.081	5.163GlyVal: 5.163 ± 0.075	0.888GlyTrp: 0.888 ± 0.036	3.239GlyTyr: 3.239 ± 0.065	0.001GlyXaa: 0.001 ± 0.001
His	1.117HisAla: 1.117 ± 0.033	0.305HisCys: 0.305 ± 0.017	0.859HisAsp: 0.859 ± 0.038	0.993HisGlu: 0.993 ± 0.035	0.926HisPhe: 0.926 ± 0.032	1.304HisGly: 1.304 ± 0.041	0.427HisHis: 0.427 ± 0.03	1.384HisIle: 1.384 ± 0.04	0.997HisLys: 0.997 ± 0.031	1.645HisLeu: 1.645 ± 0.043	0.689HisMet: 0.689 ± 0.028	0.685HisAsn: 0.685 ± 0.029	0.908HisPro: 0.908 ± 0.036	0.555HisGln: 0.555 ± 0.024	0.761HisArg: 0.761 ± 0.03	0.962HisSer: 0.962 ± 0.035	1.006HisThr: 1.006 ± 0.033	1.214HisVal: 1.214 ± 0.034	0.188HisTrp: 0.188 ± 0.014	0.768HisTyr: 0.768 ± 0.031	0.0HisXaa: 0.0 ± 0.0
Ile	5.573IleAla: 5.573 ± 0.095	1.392IleCys: 1.392 ± 0.043	3.474IleAsp: 3.474 ± 0.054	4.146IleGlu: 4.146 ± 0.074	2.797IlePhe: 2.797 ± 0.068	5.062IleGly: 5.062 ± 0.088	1.446IleHis: 1.446 ± 0.044	4.305IleIle: 4.305 ± 0.075	3.756IleLys: 3.756 ± 0.064	7.003IleLeu: 7.003 ± 0.101	1.938IleMet: 1.938 ± 0.048	2.663IleAsn: 2.663 ± 0.058	3.296IlePro: 3.296 ± 0.062	2.436IleGln: 2.436 ± 0.048	3.675IleArg: 3.675 ± 0.075	4.511IleSer: 4.511 ± 0.067	4.084IleThr: 4.084 ± 0.074	4.37IleVal: 4.37 ± 0.076	0.629IleTrp: 0.629 ± 0.026	2.555IleTyr: 2.555 ± 0.052	0.001IleXaa: 0.001 ± 0.001
Lys	5.582LysAla: 5.582 ± 0.076	0.662LysCys: 0.662 ± 0.028	4.045LysAsp: 4.045 ± 0.069	7.043LysGlu: 7.043 ± 0.106	1.775LysPhe: 1.775 ± 0.043	4.551LysGly: 4.551 ± 0.067	1.033LysHis: 1.033 ± 0.035	4.402LysIle: 4.402 ± 0.062	5.992LysLys: 5.992 ± 0.088	5.402LysLeu: 5.402 ± 0.08	2.328LysMet: 2.328 ± 0.055	3.217LysAsn: 3.217 ± 0.054	2.049LysPro: 2.049 ± 0.053	2.27LysGln: 2.27 ± 0.049	2.919LysArg: 2.919 ± 0.058	2.957LysSer: 2.957 ± 0.055	3.71LysThr: 3.71 ± 0.06	4.38LysVal: 4.38 ± 0.074	0.678LysTrp: 0.678 ± 0.03	2.442LysTyr: 2.442 ± 0.057	0.001LysXaa: 0.001 ± 0.001
Leu	7.17LeuAla: 7.17 ± 0.09	1.467LeuCys: 1.467 ± 0.039	5.143LeuAsp: 5.143 ± 0.08	6.601LeuGlu: 6.601 ± 0.106	3.986LeuPhe: 3.986 ± 0.094	6.397LeuGly: 6.397 ± 0.097	1.468LeuHis: 1.468 ± 0.035	5.695LeuIle: 5.695 ± 0.091	6.68LeuLys: 6.68 ± 0.088	8.512LeuLeu: 8.512 ± 0.142	2.842LeuMet: 2.842 ± 0.063	3.648LeuAsn: 3.648 ± 0.058	3.782LeuPro: 3.782 ± 0.073	2.605LeuGln: 2.605 ± 0.058	3.671LeuArg: 3.671 ± 0.065	6.11LeuSer: 6.11 ± 0.082	5.211LeuThr: 5.211 ± 0.081	5.746LeuVal: 5.746 ± 0.088	0.824LeuTrp: 0.824 ± 0.033	3.35LeuTyr: 3.35 ± 0.062	0.001LeuXaa: 0.001 ± 0.001
Met	3.034MetAla: 3.034 ± 0.054	0.38MetCys: 0.38 ± 0.022	2.044MetAsp: 2.044 ± 0.05	2.778MetGlu: 2.778 ± 0.053	1.041MetPhe: 1.041 ± 0.037	2.517MetGly: 2.517 ± 0.059	0.433MetHis: 0.433 ± 0.025	2.381MetIle: 2.381 ± 0.048	2.504MetLys: 2.504 ± 0.056	2.673MetLeu: 2.673 ± 0.065	1.027MetMet: 1.027 ± 0.039	1.49MetAsn: 1.49 ± 0.042	1.232MetPro: 1.232 ± 0.037	0.932MetGln: 0.932 ± 0.03	1.299MetArg: 1.299 ± 0.036	1.813MetSer: 1.813 ± 0.043	1.882MetThr: 1.882 ± 0.047	2.208MetVal: 2.208 ± 0.045	0.228MetTrp: 0.228 ± 0.015	0.881MetTyr: 0.881 ± 0.029	0.002MetXaa: 0.002 ± 0.001
Asn	2.925AsnAla: 2.925 ± 0.057	0.601AsnCys: 0.601 ± 0.027	1.94AsnAsp: 1.94 ± 0.05	2.594AsnGlu: 2.594 ± 0.058	1.489AsnPhe: 1.489 ± 0.043	3.666AsnGly: 3.666 ± 0.075	0.753AsnHis: 0.753 ± 0.027	2.982AsnIle: 2.982 ± 0.058	2.169AsnLys: 2.169 ± 0.047	3.307AsnLeu: 3.307 ± 0.07	1.299AsnMet: 1.299 ± 0.038	1.564AsnAsn: 1.564 ± 0.048	1.96AsnPro: 1.96 ± 0.044	1.371AsnGln: 1.371 ± 0.038	1.884AsnArg: 1.884 ± 0.053	2.305AsnSer: 2.305 ± 0.056	2.263AsnThr: 2.263 ± 0.049	2.828AsnVal: 2.828 ± 0.062	0.479AsnTrp: 0.479 ± 0.022	1.562AsnTyr: 1.562 ± 0.043	0.001AsnXaa: 0.001 ± 0.001
Pro	2.838ProAla: 2.838 ± 0.059	0.475ProCys: 0.475 ± 0.024	2.442ProAsp: 2.442 ± 0.055	3.608ProGlu: 3.608 ± 0.084	1.619ProPhe: 1.619 ± 0.045	2.894ProGly: 2.894 ± 0.058	0.622ProHis: 0.622 ± 0.027	1.924ProIle: 1.924 ± 0.05	1.964ProLys: 1.964 ± 0.046	3.033ProLeu: 3.033 ± 0.063	0.946ProMet: 0.946 ± 0.034	1.099ProAsn: 1.099 ± 0.031	0.773ProPro: 0.773 ± 0.031	1.156ProGln: 1.156 ± 0.037	1.011ProArg: 1.011 ± 0.04	2.006ProSer: 2.006 ± 0.047	1.558ProThr: 1.558 ± 0.041	3.602ProVal: 3.602 ± 0.061	0.369ProTrp: 0.369 ± 0.017	1.481ProTyr: 1.481 ± 0.036	0.002ProXaa: 0.002 ± 0.002
Gln	2.819GlnAla: 2.819 ± 0.06	0.414GlnCys: 0.414 ± 0.019	1.503GlnAsp: 1.503 ± 0.042	2.464GlnGlu: 2.464 ± 0.061	1.224GlnPhe: 1.224 ± 0.034	2.033GlnGly: 2.033 ± 0.046	0.387GlnHis: 0.387 ± 0.023	2.66GlnIle: 2.66 ± 0.055	2.4GlnLys: 2.4 ± 0.056	3.051GlnLeu: 3.051 ± 0.064	1.327GlnMet: 1.327 ± 0.036	1.404GlnAsn: 1.404 ± 0.041	1.046GlnPro: 1.046 ± 0.036	1.054GlnGln: 1.054 ± 0.04	1.168GlnArg: 1.168 ± 0.035	1.558GlnSer: 1.558 ± 0.043	1.702GlnThr: 1.702 ± 0.048	2.498GlnVal: 2.498 ± 0.053	0.371GlnTrp: 0.371 ± 0.02	1.333GlnTyr: 1.333 ± 0.041	0.0GlnXaa: 0.0 ± 0.0
Arg	2.613ArgAla: 2.613 ± 0.051	0.576ArgCys: 0.576 ± 0.025	2.128ArgAsp: 2.128 ± 0.053	3.538ArgGlu: 3.538 ± 0.072	1.844ArgPhe: 1.844 ± 0.046	2.409ArgGly: 2.409 ± 0.048	0.771ArgHis: 0.771 ± 0.028	3.231ArgIle: 3.231 ± 0.061	3.311ArgLys: 3.311 ± 0.05	4.007ArgLeu: 4.007 ± 0.076	1.608ArgMet: 1.608 ± 0.045	1.803ArgAsn: 1.803 ± 0.045	1.442ArgPro: 1.442 ± 0.037	1.714ArgGln: 1.714 ± 0.043	2.146ArgArg: 2.146 ± 0.057	2.333ArgSer: 2.333 ± 0.054	2.244ArgThr: 2.244 ± 0.043	2.519ArgVal: 2.519 ± 0.054	0.366ArgTrp: 0.366 ± 0.022	1.846ArgTyr: 1.846 ± 0.044	0.001ArgXaa: 0.001 ± 0.001
Ser	4.304SerAla: 4.304 ± 0.067	0.882SerCys: 0.882 ± 0.032	3.098SerAsp: 3.098 ± 0.058	3.538SerGlu: 3.538 ± 0.078	2.518SerPhe: 2.518 ± 0.058	4.99SerGly: 4.99 ± 0.082	1.106SerHis: 1.106 ± 0.034	3.809SerIle: 3.809 ± 0.072	2.967SerLys: 2.967 ± 0.056	5.401SerLeu: 5.401 ± 0.08	1.898SerMet: 1.898 ± 0.047	2.044SerAsn: 2.044 ± 0.056	1.76SerPro: 1.76 ± 0.043	2.035SerGln: 2.035 ± 0.05	2.795SerArg: 2.795 ± 0.057	3.606SerSer: 3.606 ± 0.094	2.739SerThr: 2.739 ± 0.066	4.208SerVal: 4.208 ± 0.06	0.62SerTrp: 0.62 ± 0.028	2.401SerTyr: 2.401 ± 0.054	0.0SerXaa: 0.0 ± 0.0
Thr	4.951ThrAla: 4.951 ± 0.085	0.734ThrCys: 0.734 ± 0.03	3.278ThrAsp: 3.278 ± 0.062	3.967ThrGlu: 3.967 ± 0.083	2.14ThrPhe: 2.14 ± 0.051	5.245ThrGly: 5.245 ± 0.074	0.876ThrHis: 0.876 ± 0.033	3.616ThrIle: 3.616 ± 0.062	2.885ThrLys: 2.885 ± 0.058	4.714ThrLeu: 4.714 ± 0.068	1.511ThrMet: 1.511 ± 0.044	1.795ThrAsn: 1.795 ± 0.047	2.231ThrPro: 2.231 ± 0.049	1.507ThrGln: 1.507 ± 0.039	1.961ThrArg: 1.961 ± 0.048	2.871ThrSer: 2.871 ± 0.072	2.878ThrThr: 2.878 ± 0.072	4.556ThrVal: 4.556 ± 0.077	0.538ThrTrp: 0.538 ± 0.027	1.898ThrTyr: 1.898 ± 0.051	0.001ThrXaa: 0.001 ± 0.001
Val	5.21ValAla: 5.21 ± 0.078	1.205ValCys: 1.205 ± 0.038	3.69ValAsp: 3.69 ± 0.057	4.423ValGlu: 4.423 ± 0.072	3.026ValPhe: 3.026 ± 0.064	4.403ValGly: 4.403 ± 0.095	1.135ValHis: 1.135 ± 0.034	5.377ValIle: 5.377 ± 0.088	4.697ValLys: 4.697 ± 0.083	6.962ValLeu: 6.962 ± 0.101	2.2ValMet: 2.2 ± 0.05	2.732ValAsn: 2.732 ± 0.052	2.834ValPro: 2.834 ± 0.054	1.936ValGln: 1.936 ± 0.044	2.937ValArg: 2.937 ± 0.059	4.596ValSer: 4.596 ± 0.067	4.203ValThr: 4.203 ± 0.072	4.942ValVal: 4.942 ± 0.089	0.66ValTrp: 0.66 ± 0.028	2.788ValTyr: 2.788 ± 0.062	0.0ValXaa: 0.0 ± 0.0
Trp	0.554TrpAla: 0.554 ± 0.026	0.158TrpCys: 0.158 ± 0.014	0.58TrpAsp: 0.58 ± 0.027	0.709TrpGlu: 0.709 ± 0.027	0.433TrpPhe: 0.433 ± 0.022	0.667TrpGly: 0.667 ± 0.023	0.161TrpHis: 0.161 ± 0.013	0.708TrpIle: 0.708 ± 0.028	0.832TrpLys: 0.832 ± 0.029	0.958TrpLeu: 0.958 ± 0.031	0.353TrpMet: 0.353 ± 0.022	0.505TrpAsn: 0.505 ± 0.023	0.263TrpPro: 0.263 ± 0.018	0.42TrpGln: 0.42 ± 0.02	0.399TrpArg: 0.399 ± 0.021	0.479TrpSer: 0.479 ± 0.022	0.431TrpThr: 0.431 ± 0.024	0.589TrpVal: 0.589 ± 0.028	0.125TrpTrp: 0.125 ± 0.011	0.445TrpTyr: 0.445 ± 0.03	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.691TyrAla: 2.691 ± 0.054	0.588TyrCys: 0.588 ± 0.024	2.405TyrAsp: 2.405 ± 0.056	2.986TyrGlu: 2.986 ± 0.067	1.828TyrPhe: 1.828 ± 0.051	2.992TyrGly: 2.992 ± 0.052	0.78TyrHis: 0.78 ± 0.032	2.525TyrIle: 2.525 ± 0.051	2.197TyrLys: 2.197 ± 0.048	3.617TyrLeu: 3.617 ± 0.083	1.196TyrMet: 1.196 ± 0.039	1.625TyrAsn: 1.625 ± 0.045	1.52TyrPro: 1.52 ± 0.038	1.382TyrGln: 1.382 ± 0.041	1.797TyrArg: 1.797 ± 0.044	2.263TyrSer: 2.263 ± 0.052	2.142TyrThr: 2.142 ± 0.052	2.559TyrVal: 2.559 ± 0.052	0.351TyrTrp: 0.351 ± 0.02	1.773TyrTyr: 1.773 ± 0.062	0.001TyrXaa: 0.001 ± 0.001
Xaa	0.003XaaAla: 0.003 ± 0.002	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.002XaaGly: 0.002 ± 0.001	0.001XaaHis: 0.001 ± 0.001	0.002XaaIle: 0.002 ± 0.002	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.001XaaMet: 0.001 ± 0.001	0.0XaaAsn: 0.0 ± 0.0	0.001XaaPro: 0.001 ± 0.001	0.0XaaGln: 0.0 ± 0.0	0.001XaaArg: 0.001 ± 0.001	0.0XaaSer: 0.0 ± 0.0	0.002XaaThr: 0.002 ± 0.002	0.004XaaVal: 0.004 ± 0.002	0.0XaaTrp: 0.0 ± 0.0	0.001XaaTyr: 0.001 ± 0.001	0.027XaaXaa: 0.027 ± 0.007

Statistics based on 2911 proteins (951847 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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