Kiritimatiella glycovorans
Average proteome isoelectric point is 6.42
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2373 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0G3EGF0|A0A0G3EGF0_9BACT Putative fructose-bisphosphate aldolase OS=Kiritimatiella glycovorans OX=1307763 GN=fbaA PE=4 SV=1
MM1 pKa = 7.75 RR2 pKa = 11.84 AFFSAACFTCLAFGAVSAHH21 pKa = 5.52 AVSVTTTSLPTGAVAEE37 pKa = 4.85 AYY39 pKa = 10.45 SGTLSASGGTTPYY52 pKa = 9.95 RR53 pKa = 11.84 WNVAQDD59 pKa = 3.59 YY60 pKa = 11.03 AEE62 pKa = 4.62 SIEE65 pKa = 4.34 TNAMQDD71 pKa = 3.3 PSADD75 pKa = 3.62 MDD77 pKa = 3.69 WNADD81 pKa = 3.48 SEE83 pKa = 4.63 AWLLDD88 pKa = 3.56 LPFTFPFYY96 pKa = 11.01 GVGYY100 pKa = 5.11 TQCWVAVDD108 pKa = 4.91 GYY110 pKa = 10.13 VHH112 pKa = 7.37 FATNYY117 pKa = 10.27 LDD119 pKa = 3.74 STPTTNEE126 pKa = 3.56 LAQQCMIAADD136 pKa = 3.93 WKK138 pKa = 11.24 DD139 pKa = 3.57 LDD141 pKa = 3.93 TSAGGDD147 pKa = 3.42 LDD149 pKa = 3.9 IYY151 pKa = 10.74 IWTNDD156 pKa = 3.31 SSCVIRR162 pKa = 11.84 WYY164 pKa = 10.81 GRR166 pKa = 11.84 TPMWQYY172 pKa = 11.76 VGIKK176 pKa = 10.5 LEE178 pKa = 4.11 MNEE181 pKa = 4.04 NGLIRR186 pKa = 11.84 VDD188 pKa = 3.53 MSGDD192 pKa = 3.31 EE193 pKa = 4.64 GMMSPRR199 pKa = 11.84 FCGLSAGNGVDD210 pKa = 3.8 YY211 pKa = 11.13 VVSTNNDD218 pKa = 2.47 AWLDD222 pKa = 3.65 PGDD225 pKa = 4.14 IIRR228 pKa = 11.84 FQPEE232 pKa = 3.63 DD233 pKa = 3.76 ALPTNIVMTSGGEE246 pKa = 4.06 LYY248 pKa = 8.13 GTPWNAGTNSLTFRR262 pKa = 11.84 VTDD265 pKa = 3.53 ATNGQAAAALDD276 pKa = 3.81 LVVTEE281 pKa = 5.07 GGNAKK286 pKa = 9.82 PVVSGSTPAAGAYY299 pKa = 9.83 PMDD302 pKa = 3.98 EE303 pKa = 4.65 AEE305 pKa = 4.39 TNTFSVAASDD315 pKa = 4.11 PDD317 pKa = 4.05 GTPLSYY323 pKa = 11.09 AWTLDD328 pKa = 3.22 GTAVGTNSASYY339 pKa = 11.25 DD340 pKa = 3.41 FGTPWGGAGVYY351 pKa = 9.01 TLLVEE356 pKa = 4.69 ISDD359 pKa = 4.62 DD360 pKa = 3.33 FWTNGEE366 pKa = 4.36 VSVEE370 pKa = 3.7 WTVAVTNDD378 pKa = 3.13 NDD380 pKa = 4.14 GDD382 pKa = 3.88 GMLNEE387 pKa = 4.21 WEE389 pKa = 4.16 RR390 pKa = 11.84 TYY392 pKa = 11.72 GLDD395 pKa = 4.52 PEE397 pKa = 5.02 VNDD400 pKa = 4.85 SGGDD404 pKa = 3.51 GDD406 pKa = 6.01 GDD408 pKa = 3.58 GLSNIEE414 pKa = 4.01 EE415 pKa = 4.41 HH416 pKa = 7.66 DD417 pKa = 4.0 AGTDD421 pKa = 3.47 PGSEE425 pKa = 4.03 DD426 pKa = 3.49 TDD428 pKa = 3.61 NDD430 pKa = 3.76 TLYY433 pKa = 11.24 DD434 pKa = 3.9 DD435 pKa = 4.22 WEE437 pKa = 4.63 LEE439 pKa = 4.02 YY440 pKa = 10.75 GYY442 pKa = 10.9 DD443 pKa = 3.44 PTSPSEE449 pKa = 3.97 ILPGYY454 pKa = 8.21 TNYY457 pKa = 9.8 TPSYY461 pKa = 9.53 GYY463 pKa = 10.52 NLSADD468 pKa = 3.61 AEE470 pKa = 4.39 EE471 pKa = 5.24 VYY473 pKa = 9.42 VTGTTAYY480 pKa = 9.43 VACDD484 pKa = 3.28 VGGLKK489 pKa = 10.68 VIDD492 pKa = 4.98 CSDD495 pKa = 3.54 PDD497 pKa = 4.11 APVLIDD503 pKa = 3.13 MHH505 pKa = 6.81 EE506 pKa = 4.04 IPGRR510 pKa = 11.84 AEE512 pKa = 4.31 EE513 pKa = 4.18 ICGLGPYY520 pKa = 10.08 VFVGADD526 pKa = 3.31 NYY528 pKa = 11.59 GLTIFDD534 pKa = 4.59 ASTPSNLAVLCTWTNAHH551 pKa = 7.02 LFYY554 pKa = 11.22 GPMAAVGDD562 pKa = 4.15 RR563 pKa = 11.84 LYY565 pKa = 11.06 VAQNSDD571 pKa = 3.78 LTVLSISDD579 pKa = 3.76 PASPVLLGQLDD590 pKa = 3.96 VSNTNHH596 pKa = 7.36 PAPGSIRR603 pKa = 11.84 DD604 pKa = 3.47 VAVRR608 pKa = 11.84 GDD610 pKa = 3.56 YY611 pKa = 10.47 AYY613 pKa = 10.72 CANFTEE619 pKa = 4.61 GLKK622 pKa = 9.34 TVNISDD628 pKa = 4.5 PEE630 pKa = 4.44 TMVITATNEE639 pKa = 3.81 YY640 pKa = 9.76 EE641 pKa = 4.29 GVGVCEE647 pKa = 3.94 YY648 pKa = 10.79 AARR651 pKa = 11.84 GLDD654 pKa = 3.49 IEE656 pKa = 5.05 GDD658 pKa = 4.09 TLFMCQDD665 pKa = 3.76 GYY667 pKa = 12.0 GLMAFDD673 pKa = 5.48 LSDD676 pKa = 3.91 PASPALIDD684 pKa = 4.21 NFISSHH690 pKa = 6.15 NPFPYY695 pKa = 10.53 DD696 pKa = 3.24 NLDD699 pKa = 3.81 DD700 pKa = 4.28 VDD702 pKa = 4.48 VEE704 pKa = 4.38 GTNAYY709 pKa = 10.25 VIGGKK714 pKa = 9.62 RR715 pKa = 11.84 FWIYY719 pKa = 10.86 GVSDD723 pKa = 4.13 PSNMVEE729 pKa = 4.15 LAHH732 pKa = 6.03 WQNGSGGPTDD742 pKa = 4.98 GIGIHH747 pKa = 7.09 DD748 pKa = 4.22 VDD750 pKa = 4.18 AEE752 pKa = 4.38 GDD754 pKa = 4.18 FVHH757 pKa = 7.09 LPTGWGTYY765 pKa = 7.36 TNAYY769 pKa = 9.15 GIQVDD774 pKa = 3.73 RR775 pKa = 11.84 GGLRR779 pKa = 11.84 LMDD782 pKa = 4.09 VTDD785 pKa = 3.85 KK786 pKa = 11.13 AAPEE790 pKa = 3.67 PRR792 pKa = 11.84 GRR794 pKa = 11.84 FITSGQGWDD803 pKa = 3.46 MEE805 pKa = 4.34 ILNDD809 pKa = 3.37 VAYY812 pKa = 10.1 VANYY816 pKa = 9.25 HH817 pKa = 6.02 SGVHH821 pKa = 5.48 IVNVTNWTRR830 pKa = 11.84 LGRR833 pKa = 11.84 YY834 pKa = 6.49 DD835 pKa = 3.55 TEE837 pKa = 5.15 GIAMGIDD844 pKa = 3.45 VTDD847 pKa = 4.02 GGDD850 pKa = 3.38 TLLVADD856 pKa = 5.06 GEE858 pKa = 4.5 NGLVVLDD865 pKa = 4.04 ATDD868 pKa = 4.08 PANMALQSHH877 pKa = 7.45 LDD879 pKa = 3.55 TDD881 pKa = 3.68 DD882 pKa = 3.65 ARR884 pKa = 11.84 AVRR887 pKa = 11.84 YY888 pKa = 9.83 LNGRR892 pKa = 11.84 IYY894 pKa = 10.8 VADD897 pKa = 3.8 YY898 pKa = 11.22 TNGLLVATNLSVPTLQTNFFAGSPIQGLTTYY929 pKa = 10.44 GSKK932 pKa = 10.61 VYY934 pKa = 10.37 AAAGGLGVYY943 pKa = 9.52 SVYY946 pKa = 10.83 CEE948 pKa = 3.82 EE949 pKa = 4.59 KK950 pKa = 10.46 GSNEE954 pKa = 3.77 TWHH957 pKa = 6.36 TEE959 pKa = 3.65 HH960 pKa = 6.76 YY961 pKa = 10.7 NYY963 pKa = 9.75 TIDD966 pKa = 3.51 MGKK969 pKa = 9.58 SASAYY974 pKa = 9.81 RR975 pKa = 11.84 LEE977 pKa = 4.45 AADD980 pKa = 3.42 GRR982 pKa = 11.84 LWVADD987 pKa = 3.57 NLNGVVAIDD996 pKa = 3.59 ISSAAMSYY1004 pKa = 10.62 EE1005 pKa = 3.63 GLYY1008 pKa = 10.24 TNGVVQNVFIEE1019 pKa = 4.23 DD1020 pKa = 3.61 HH1021 pKa = 6.94 APTGDD1026 pKa = 4.02 YY1027 pKa = 10.42 IWIGGGTNITSFRR1040 pKa = 11.84 VGDD1043 pKa = 4.36 PIDD1046 pKa = 3.76 RR1047 pKa = 11.84 RR1048 pKa = 11.84 NTMSDD1053 pKa = 3.63 EE1054 pKa = 4.3 YY1055 pKa = 9.87 WWQDD1059 pKa = 3.77 PEE1061 pKa = 5.87 IKK1063 pKa = 10.41 DD1064 pKa = 3.28 LWFAGTNGIFLGATQWKK1081 pKa = 9.1 KK1082 pKa = 10.62 YY1083 pKa = 10.03 EE1084 pKa = 3.83 YY1085 pKa = 10.78 GLRR1088 pKa = 11.84 SFNTTRR1094 pKa = 11.84 VDD1096 pKa = 3.39 ADD1098 pKa = 3.72 EE1099 pKa = 6.63 DD1100 pKa = 3.94 GMADD1104 pKa = 3.58 SDD1106 pKa = 3.84 EE1107 pKa = 4.12 MRR1109 pKa = 11.84 WWGSTVPGRR1118 pKa = 11.84 YY1119 pKa = 9.47 DD1120 pKa = 3.31 DD1121 pKa = 3.75 TDD1123 pKa = 4.41 GDD1125 pKa = 4.28 GLSNWGEE1132 pKa = 4.38 STTGTDD1138 pKa = 4.17 PSLTDD1143 pKa = 3.18 TDD1145 pKa = 4.01 GDD1147 pKa = 4.25 GVSDD1151 pKa = 3.81 RR1152 pKa = 11.84 DD1153 pKa = 3.62 EE1154 pKa = 4.91 YY1155 pKa = 11.09 IAGTDD1160 pKa = 3.54 PMSDD1164 pKa = 3.88 GSCFSMDD1171 pKa = 3.83 LDD1173 pKa = 4.22 DD1174 pKa = 5.54 PVYY1177 pKa = 10.78 HH1178 pKa = 7.35 LDD1180 pKa = 3.9 LFDD1183 pKa = 4.32 EE1184 pKa = 5.65 FVLRR1188 pKa = 11.84 WPSRR1192 pKa = 11.84 AGRR1195 pKa = 11.84 VYY1197 pKa = 11.15 DD1198 pKa = 3.81 LYY1200 pKa = 10.85 RR1201 pKa = 11.84 YY1202 pKa = 10.17 SDD1204 pKa = 3.85 LGDD1207 pKa = 3.59 PSSRR1211 pKa = 11.84 VQLLTNAPATPPMNIYY1227 pKa = 9.3 TDD1229 pKa = 3.45 TVFRR1233 pKa = 11.84 IRR1235 pKa = 11.84 SYY1237 pKa = 9.09 MYY1239 pKa = 9.38 RR1240 pKa = 11.84 INVEE1244 pKa = 3.99 RR1245 pKa = 11.84 EE1246 pKa = 3.67
Molecular weight: 134.99 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.769
IPC2_protein 3.757
IPC_protein 3.821
Toseland 3.579
ProMoST 3.973
Dawson 3.821
Bjellqvist 3.973
Wikipedia 3.757
Rodwell 3.643
Grimsley 3.49
Solomon 3.808
Lehninger 3.77
Nozaki 3.923
DTASelect 4.202
Thurlkill 3.63
EMBOSS 3.757
Sillero 3.935
Patrickios 0.451
IPC_peptide 3.808
IPC2_peptide 3.923
IPC2.peptide.svr19 3.849
Protein with the highest isoelectric point:
>tr|A0A0G3ED69|A0A0G3ED69_9BACT Twitching mobility protein OS=Kiritimatiella glycovorans OX=1307763 GN=pilT_2 PE=3 SV=1
MM1 pKa = 7.4 IAGGRR6 pKa = 11.84 IQVNGEE12 pKa = 4.32 TVCEE16 pKa = 3.83 QGIKK20 pKa = 9.97 VDD22 pKa = 4.11 PVQDD26 pKa = 4.67 DD27 pKa = 2.77 IRR29 pKa = 11.84 MDD31 pKa = 3.17 GRR33 pKa = 11.84 VVRR36 pKa = 11.84 RR37 pKa = 11.84 EE38 pKa = 3.99 SKK40 pKa = 10.32 VVYY43 pKa = 10.06 ILNKK47 pKa = 9.56 PPGVMCTNDD56 pKa = 3.92 DD57 pKa = 3.75 PQSRR61 pKa = 11.84 PLYY64 pKa = 9.97 RR65 pKa = 11.84 DD66 pKa = 4.28 LFPADD71 pKa = 3.63 AGRR74 pKa = 11.84 LFPVGRR80 pKa = 11.84 LDD82 pKa = 4.48 CMSEE86 pKa = 3.87 GLLLVTNDD94 pKa = 2.63 GDD96 pKa = 3.87 LAHH99 pKa = 7.42 RR100 pKa = 11.84 LMHH103 pKa = 6.29 PRR105 pKa = 11.84 YY106 pKa = 9.09 GFHH109 pKa = 6.19 KK110 pKa = 8.95 TYY112 pKa = 10.6 LARR115 pKa = 11.84 TPDD118 pKa = 3.43 RR119 pKa = 11.84 VTAEE123 pKa = 3.47 QMKK126 pKa = 10.42 RR127 pKa = 11.84 MEE129 pKa = 4.19 QGVEE133 pKa = 3.8 EE134 pKa = 4.53 GGEE137 pKa = 3.88 RR138 pKa = 11.84 LRR140 pKa = 11.84 ALRR143 pKa = 11.84 VRR145 pKa = 11.84 RR146 pKa = 11.84 EE147 pKa = 3.76 RR148 pKa = 11.84 GAGPGTRR155 pKa = 11.84 LRR157 pKa = 11.84 IVLGEE162 pKa = 3.61 GRR164 pKa = 11.84 NRR166 pKa = 11.84 HH167 pKa = 4.64 IRR169 pKa = 11.84 RR170 pKa = 11.84 MFKK173 pKa = 10.71 ALGLRR178 pKa = 11.84 IDD180 pKa = 3.35 RR181 pKa = 11.84 LKK183 pKa = 10.63 RR184 pKa = 11.84 VKK186 pKa = 10.51 LGPLVLGGLPQGSWRR201 pKa = 11.84 EE202 pKa = 3.86 LRR204 pKa = 11.84 PQEE207 pKa = 4.28 LRR209 pKa = 11.84 QLQVSSHH216 pKa = 5.17 QRR218 pKa = 11.84 DD219 pKa = 3.27 AA220 pKa = 4.25
Molecular weight: 25.03 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.35
IPC2_protein 9.472
IPC_protein 10.438
Toseland 10.657
ProMoST 10.452
Dawson 10.73
Bjellqvist 10.496
Wikipedia 10.994
Rodwell 10.789
Grimsley 10.789
Solomon 10.906
Lehninger 10.862
Nozaki 10.643
DTASelect 10.482
Thurlkill 10.657
EMBOSS 11.067
Sillero 10.687
Patrickios 10.496
IPC_peptide 10.921
IPC2_peptide 9.633
IPC2.peptide.svr19 8.731
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2373
0
2373
860530
29
1991
362.6
40.29
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
10.122 ± 0.054
1.255 ± 0.018
6.006 ± 0.045
7.464 ± 0.048
3.814 ± 0.027
8.514 ± 0.045
2.348 ± 0.024
4.606 ± 0.033
3.111 ± 0.042
9.621 ± 0.061
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.411 ± 0.022
2.636 ± 0.033
5.265 ± 0.036
2.697 ± 0.027
8.854 ± 0.055
5.058 ± 0.034
4.965 ± 0.037
6.95 ± 0.039
1.496 ± 0.024
2.807 ± 0.031
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here