Anaerosporomusa subterranea
Average proteome isoelectric point is 6.53
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3585 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A154BSH0|A0A154BSH0_ANASB GDSL family lipase OS=Anaerosporomusa subterranea OX=1794912 GN=AXX12_02080 PE=4 SV=1
MM1 pKa = 7.71 KK2 pKa = 10.53 LGDD5 pKa = 4.1 LSAKK9 pKa = 10.29 YY10 pKa = 9.86 EE11 pKa = 4.59 SNGDD15 pKa = 3.48 PGAISSGEE23 pKa = 3.75 GDD25 pKa = 3.43 AGGVSYY31 pKa = 10.7 GAYY34 pKa = 9.8 QFAANAGVPGQFVAWLKK51 pKa = 10.62 QIGYY55 pKa = 10.01 LYY57 pKa = 10.87 ADD59 pKa = 4.19 EE60 pKa = 4.6 LAEE63 pKa = 4.62 AGVPGCDD70 pKa = 3.27 EE71 pKa = 5.88 FSDD74 pKa = 3.22 AWLRR78 pKa = 11.84 AAARR82 pKa = 11.84 DD83 pKa = 3.57 PDD85 pKa = 3.9 GFLAAQHH92 pKa = 5.58 EE93 pKa = 5.0 FVRR96 pKa = 11.84 QSYY99 pKa = 8.87 YY100 pKa = 10.88 EE101 pKa = 3.97 PARR104 pKa = 11.84 EE105 pKa = 3.65 QALAAGINIDD115 pKa = 3.56 GCSFALQNVVWSAAVQYY132 pKa = 9.93 GAYY135 pKa = 9.03 YY136 pKa = 10.41 VKK138 pKa = 10.68 EE139 pKa = 4.17 LFEE142 pKa = 4.99 DD143 pKa = 3.89 AATQLGVTSAADD155 pKa = 3.59 ADD157 pKa = 4.13 DD158 pKa = 4.12 AALIQAIYY166 pKa = 10.23 DD167 pKa = 3.53 VRR169 pKa = 11.84 ASDD172 pKa = 3.37 EE173 pKa = 4.22 WTTGSPEE180 pKa = 4.08 LRR182 pKa = 11.84 PGLIARR188 pKa = 11.84 FEE190 pKa = 4.28 AEE192 pKa = 4.65 CRR194 pKa = 11.84 DD195 pKa = 3.55 ALAALDD201 pKa = 4.12 SEE203 pKa = 4.82
Molecular weight: 21.53 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.759
IPC2_protein 3.973
IPC_protein 3.948
Toseland 3.745
ProMoST 4.062
Dawson 3.923
Bjellqvist 4.113
Wikipedia 3.834
Rodwell 3.77
Grimsley 3.656
Solomon 3.91
Lehninger 3.872
Nozaki 4.037
DTASelect 4.24
Thurlkill 3.783
EMBOSS 3.846
Sillero 4.062
Patrickios 1.138
IPC_peptide 3.91
IPC2_peptide 4.037
IPC2.peptide.svr19 3.961
Protein with the highest isoelectric point:
>tr|A0A154BP56|A0A154BP56_ANASB 4-hydroxy-tetrahydrodipicolinate synthase OS=Anaerosporomusa subterranea OX=1794912 GN=dapA PE=3 SV=1
MM1 pKa = 7.44 KK2 pKa = 9.52 RR3 pKa = 11.84 TFQPNVSWRR12 pKa = 11.84 KK13 pKa = 6.99 KK14 pKa = 6.42 THH16 pKa = 5.13 GFRR19 pKa = 11.84 EE20 pKa = 4.31 RR21 pKa = 11.84 MSSLGGRR28 pKa = 11.84 QVLKK32 pKa = 10.45 RR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 10.19 GRR39 pKa = 11.84 KK40 pKa = 8.79 KK41 pKa = 10.63 LSAA44 pKa = 3.95
Molecular weight: 5.26 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.381
IPC2_protein 10.818
IPC_protein 12.384
Toseland 12.559
ProMoST 13.042
Dawson 12.559
Bjellqvist 12.544
Wikipedia 13.027
Rodwell 12.34
Grimsley 12.603
Solomon 13.056
Lehninger 12.954
Nozaki 12.559
DTASelect 12.544
Thurlkill 12.559
EMBOSS 13.056
Sillero 12.559
Patrickios 12.076
IPC_peptide 13.056
IPC2_peptide 12.032
IPC2.peptide.svr19 9.048
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3585
0
3585
1103724
37
1441
307.9
33.84
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.548 ± 0.045
1.162 ± 0.017
4.949 ± 0.031
5.992 ± 0.035
3.895 ± 0.028
7.666 ± 0.037
1.875 ± 0.017
7.248 ± 0.035
5.298 ± 0.032
10.162 ± 0.037
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.739 ± 0.021
3.702 ± 0.024
4.07 ± 0.024
3.851 ± 0.028
4.937 ± 0.031
5.817 ± 0.028
5.454 ± 0.026
7.698 ± 0.032
0.942 ± 0.016
2.994 ± 0.024
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here