Ferrimonas marina
Average proteome isoelectric point is 6.04
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4625 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1M5Y098|A0A1M5Y098_9GAMM Uncharacterized protein OS=Ferrimonas marina OX=299255 GN=SAMN02745129_3842 PE=4 SV=1
MM1 pKa = 7.73 HH2 pKa = 7.23 YY3 pKa = 8.38 NTSEE7 pKa = 3.97 LCDD10 pKa = 4.12 LYY12 pKa = 11.24 GDD14 pKa = 4.04 QIDD17 pKa = 3.89 VVEE20 pKa = 4.47 PMFSNFGGCPSFGGQIHH37 pKa = 5.36 TVKK40 pKa = 10.62 CFEE43 pKa = 5.1 DD44 pKa = 3.41 NSAIAEE50 pKa = 4.06 ALEE53 pKa = 3.75 QDD55 pKa = 3.92 GQGKK59 pKa = 9.01 VLLVDD64 pKa = 4.2 GGGSMRR70 pKa = 11.84 RR71 pKa = 11.84 ALVDD75 pKa = 3.33 QEE77 pKa = 4.37 LANLAVSNQWEE88 pKa = 4.26 GLVIYY93 pKa = 10.37 GSVRR97 pKa = 11.84 DD98 pKa = 3.79 VDD100 pKa = 3.93 ALEE103 pKa = 5.12 DD104 pKa = 3.92 LDD106 pKa = 4.68 LGIMALASIPVGASQHH122 pKa = 6.58 GEE124 pKa = 3.99 GQLDD128 pKa = 3.65 VPVNFGGVTFMPEE141 pKa = 3.08 DD142 pKa = 3.61 HH143 pKa = 7.12 LYY145 pKa = 10.82 VDD147 pKa = 3.54 STGIILSEE155 pKa = 4.47 DD156 pKa = 3.65 PLDD159 pKa = 4.28 IEE161 pKa = 4.75
Molecular weight: 17.25 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.707
IPC2_protein 3.77
IPC_protein 3.757
Toseland 3.541
ProMoST 3.897
Dawson 3.745
Bjellqvist 3.935
Wikipedia 3.681
Rodwell 3.579
Grimsley 3.452
Solomon 3.732
Lehninger 3.694
Nozaki 3.859
DTASelect 4.075
Thurlkill 3.605
EMBOSS 3.694
Sillero 3.872
Patrickios 0.985
IPC_peptide 3.732
IPC2_peptide 3.846
IPC2.peptide.svr19 3.795
Protein with the highest isoelectric point:
>tr|A0A1M5ZL78|A0A1M5ZL78_9GAMM Exoribonuclease 2 OS=Ferrimonas marina OX=299255 GN=rnb PE=3 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.51 RR3 pKa = 11.84 TFQPSVLKK11 pKa = 10.46 RR12 pKa = 11.84 KK13 pKa = 9.37 RR14 pKa = 11.84 NHH16 pKa = 5.37 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MATKK25 pKa = 10.4 NGRR28 pKa = 11.84 QVLARR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 10.39 GRR39 pKa = 11.84 ARR41 pKa = 11.84 LSAA44 pKa = 3.91
Molecular weight: 5.16 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.523
IPC2_protein 11.242
IPC_protein 12.837
Toseland 13.013
ProMoST 13.495
Dawson 13.013
Bjellqvist 12.998
Wikipedia 13.481
Rodwell 12.676
Grimsley 13.042
Solomon 13.495
Lehninger 13.408
Nozaki 13.013
DTASelect 12.998
Thurlkill 13.013
EMBOSS 13.51
Sillero 13.013
Patrickios 12.398
IPC_peptide 13.51
IPC2_peptide 12.486
IPC2.peptide.svr19 9.177
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4625
0
4625
1462360
35
2489
316.2
34.84
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
10.432 ± 0.043
1.05 ± 0.015
5.574 ± 0.033
6.155 ± 0.037
3.568 ± 0.024
7.73 ± 0.029
2.362 ± 0.02
4.542 ± 0.029
3.439 ± 0.032
11.471 ± 0.054
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.577 ± 0.018
3.169 ± 0.024
4.774 ± 0.027
5.421 ± 0.043
5.803 ± 0.035
5.847 ± 0.032
4.912 ± 0.031
6.936 ± 0.032
1.477 ± 0.018
2.76 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here