Escherichia virus Qbeta (Bacteriophage Q-beta)

Taxonomy: Viruses; Riboviria; Orthornavirae; Lenarviricota; Allassoviricetes; Levivirales; Leviviridae; Allolevivirus

Average proteome isoelectric point is 7.61

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>sp|Q8LTE1|A1_BPQBE Minor capsid protein A1 OS=Escherichia virus Qbeta OX=39803 PE=1 SV=2
MM1 pKa = 7.56SKK3 pKa = 8.7TASSRR8 pKa = 11.84NSLSAQLRR16 pKa = 11.84RR17 pKa = 11.84AANTRR22 pKa = 11.84IEE24 pKa = 4.23VEE26 pKa = 4.16GNLALSIANDD36 pKa = 3.57LLLAYY41 pKa = 8.92GQSPFNSEE49 pKa = 4.07AEE51 pKa = 4.56CISFSPRR58 pKa = 11.84FDD60 pKa = 3.21GTPDD64 pKa = 3.48DD65 pKa = 5.01FRR67 pKa = 11.84INYY70 pKa = 8.89LKK72 pKa = 10.96AEE74 pKa = 4.19IMSKK78 pKa = 10.94YY79 pKa = 10.64DD80 pKa = 3.86DD81 pKa = 4.1FSLGIDD87 pKa = 3.6TEE89 pKa = 4.29AVAWEE94 pKa = 4.23KK95 pKa = 10.73FLAAEE100 pKa = 4.51AEE102 pKa = 4.45CALTNARR109 pKa = 11.84LYY111 pKa = 10.84RR112 pKa = 11.84PDD114 pKa = 3.36YY115 pKa = 11.43SEE117 pKa = 5.66DD118 pKa = 3.67FNFSLGEE125 pKa = 4.13SCIHH129 pKa = 5.12MARR132 pKa = 11.84RR133 pKa = 11.84KK134 pKa = 8.54IAKK137 pKa = 9.89LIGDD141 pKa = 3.94VPSVEE146 pKa = 3.85GMLRR150 pKa = 11.84HH151 pKa = 6.06CRR153 pKa = 11.84FSGGATTTNNRR164 pKa = 11.84SYY166 pKa = 11.02GHH168 pKa = 7.13PSFKK172 pKa = 10.34FALPQACTPRR182 pKa = 11.84ALKK185 pKa = 10.57YY186 pKa = 10.64VLALRR191 pKa = 11.84ASTHH195 pKa = 4.78FDD197 pKa = 2.69IRR199 pKa = 11.84ISDD202 pKa = 3.66ISPFNKK208 pKa = 10.01AVTVPKK214 pKa = 10.41NSKK217 pKa = 7.99TDD219 pKa = 3.07RR220 pKa = 11.84CIAIEE225 pKa = 4.3PGWNMFFQLGIGGILRR241 pKa = 11.84DD242 pKa = 4.47RR243 pKa = 11.84LRR245 pKa = 11.84CWGIDD250 pKa = 4.63LNDD253 pKa = 3.14QTINQRR259 pKa = 11.84RR260 pKa = 11.84AHH262 pKa = 6.4EE263 pKa = 4.41GSVTNNLATVDD274 pKa = 4.27LSAASDD280 pKa = 4.1SISLALCEE288 pKa = 4.79LLLPPGWFEE297 pKa = 4.03VLMDD301 pKa = 4.06LRR303 pKa = 11.84SPKK306 pKa = 10.13GRR308 pKa = 11.84LPDD311 pKa = 3.84GSVVTYY317 pKa = 9.8EE318 pKa = 5.13KK319 pKa = 10.24ISSMGNGYY327 pKa = 7.26TFEE330 pKa = 4.9LEE332 pKa = 4.21SLIFASLARR341 pKa = 11.84SVCEE345 pKa = 4.6ILDD348 pKa = 4.11LDD350 pKa = 3.97SSEE353 pKa = 4.05VTVYY357 pKa = 11.02GDD359 pKa = 5.34DD360 pKa = 4.8IILPSCAVPALRR372 pKa = 11.84EE373 pKa = 3.85VFKK376 pKa = 11.39YY377 pKa = 10.92VGFTTNTKK385 pKa = 8.63KK386 pKa = 9.18TFSEE390 pKa = 5.37GPFRR394 pKa = 11.84EE395 pKa = 4.49SCGKK399 pKa = 9.83HH400 pKa = 5.46YY401 pKa = 10.94YY402 pKa = 10.3SGVDD406 pKa = 3.12VTPFYY411 pKa = 10.39IRR413 pKa = 11.84HH414 pKa = 6.47RR415 pKa = 11.84IVSPADD421 pKa = 4.01LILVLNNLYY430 pKa = 10.17RR431 pKa = 11.84WATIDD436 pKa = 4.29GVWDD440 pKa = 3.98PRR442 pKa = 11.84AHH444 pKa = 6.3SVYY447 pKa = 10.64LKK449 pKa = 10.35YY450 pKa = 10.51RR451 pKa = 11.84KK452 pKa = 9.32LLPKK456 pKa = 9.88QLQRR460 pKa = 11.84NTIPDD465 pKa = 4.08GYY467 pKa = 11.09GDD469 pKa = 3.92GALVGSVLINPFAKK483 pKa = 9.42NRR485 pKa = 11.84GWIRR489 pKa = 11.84YY490 pKa = 7.93VPVITDD496 pKa = 3.28HH497 pKa = 5.71TRR499 pKa = 11.84DD500 pKa = 3.36RR501 pKa = 11.84EE502 pKa = 4.05RR503 pKa = 11.84AEE505 pKa = 4.27LGSYY509 pKa = 10.56LYY511 pKa = 10.85DD512 pKa = 3.66LFSRR516 pKa = 11.84CLSEE520 pKa = 5.64SNDD523 pKa = 3.45GLPLRR528 pKa = 11.84GPSGCDD534 pKa = 3.0SADD537 pKa = 3.31LFAIDD542 pKa = 5.07QLICRR547 pKa = 11.84SNPTKK552 pKa = 10.4ISRR555 pKa = 11.84STGKK559 pKa = 10.29FDD561 pKa = 3.18IQYY564 pKa = 8.74IACSSRR570 pKa = 11.84VLAPYY575 pKa = 10.57GVFQGTKK582 pKa = 9.16VASLHH587 pKa = 5.47EE588 pKa = 4.26AA589 pKa = 3.36

Molecular weight: 65.53 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>sp|Q8LTE2|MATA2_BPQBE Maturation protein A2 OS=Escherichia virus Qbeta OX=39803 PE=1 SV=1
MM1 pKa = 7.71PKK3 pKa = 10.23LPRR6 pKa = 11.84GLRR9 pKa = 11.84FGADD13 pKa = 3.24NEE15 pKa = 4.22ILNDD19 pKa = 4.03FQEE22 pKa = 4.27LWFPDD27 pKa = 3.71LFIEE31 pKa = 5.64SSDD34 pKa = 3.31THH36 pKa = 5.46PWYY39 pKa = 9.22TLKK42 pKa = 10.9GRR44 pKa = 11.84VLNAHH49 pKa = 6.9LDD51 pKa = 3.64DD52 pKa = 4.99RR53 pKa = 11.84LPNVGGRR60 pKa = 11.84QVRR63 pKa = 11.84RR64 pKa = 11.84TPHH67 pKa = 6.39RR68 pKa = 11.84VTVPIASSGLRR79 pKa = 11.84PVTTVQYY86 pKa = 11.01DD87 pKa = 3.63PAALSFLLNARR98 pKa = 11.84VDD100 pKa = 3.28WDD102 pKa = 3.98FGNGDD107 pKa = 3.75SANLVINDD115 pKa = 3.19FLFRR119 pKa = 11.84TFAPKK124 pKa = 10.55EE125 pKa = 3.84FDD127 pKa = 3.51FSNSLVPRR135 pKa = 11.84YY136 pKa = 7.01TQAFSAFNAKK146 pKa = 9.28YY147 pKa = 8.72GTMIGEE153 pKa = 4.21GLEE156 pKa = 4.28TIKK159 pKa = 11.09YY160 pKa = 10.14LGLLLRR166 pKa = 11.84RR167 pKa = 11.84LRR169 pKa = 11.84EE170 pKa = 3.85GYY172 pKa = 9.97RR173 pKa = 11.84AVKK176 pKa = 10.45RR177 pKa = 11.84GDD179 pKa = 3.12LRR181 pKa = 11.84ALRR184 pKa = 11.84RR185 pKa = 11.84VIQSYY190 pKa = 11.34HH191 pKa = 5.03NGKK194 pKa = 8.62WKK196 pKa = 9.67PATAGNLWLEE206 pKa = 4.05FRR208 pKa = 11.84YY209 pKa = 10.88GLMPLFYY216 pKa = 10.44DD217 pKa = 3.88IRR219 pKa = 11.84DD220 pKa = 3.92VMLDD224 pKa = 3.02WQNRR228 pKa = 11.84HH229 pKa = 6.37DD230 pKa = 4.62KK231 pKa = 10.31IQRR234 pKa = 11.84LLRR237 pKa = 11.84FSVGHH242 pKa = 6.04GEE244 pKa = 4.23DD245 pKa = 3.78YY246 pKa = 11.19VVEE249 pKa = 4.32FDD251 pKa = 3.66NLYY254 pKa = 10.04PAVAYY259 pKa = 8.42FKK261 pKa = 11.07LKK263 pKa = 11.18GEE265 pKa = 3.9ITLEE269 pKa = 3.51RR270 pKa = 11.84RR271 pKa = 11.84HH272 pKa = 5.59RR273 pKa = 11.84HH274 pKa = 5.18GISYY278 pKa = 10.79ANRR281 pKa = 11.84EE282 pKa = 4.21GYY284 pKa = 10.74AVFDD288 pKa = 4.06NGSLRR293 pKa = 11.84PVSDD297 pKa = 3.0WKK299 pKa = 10.98EE300 pKa = 3.52LATAFINPHH309 pKa = 4.5EE310 pKa = 4.7VAWEE314 pKa = 3.73LTPYY318 pKa = 10.72SFVVDD323 pKa = 3.51WFLNVGDD330 pKa = 4.94ILAQQGQLYY339 pKa = 10.14HH340 pKa = 6.89NIDD343 pKa = 3.27IVDD346 pKa = 3.7GFDD349 pKa = 3.84RR350 pKa = 11.84RR351 pKa = 11.84DD352 pKa = 3.05IRR354 pKa = 11.84LKK356 pKa = 10.49SFTIKK361 pKa = 10.67GEE363 pKa = 4.24RR364 pKa = 11.84NGRR367 pKa = 11.84PVNVSASLSAVDD379 pKa = 4.34LFYY382 pKa = 11.51SRR384 pKa = 11.84LHH386 pKa = 5.8TSNLPFATLDD396 pKa = 3.44LDD398 pKa = 3.97TTFSSFKK405 pKa = 10.41HH406 pKa = 5.3VLDD409 pKa = 4.96SIFLLTQRR417 pKa = 11.84VKK419 pKa = 10.83RR420 pKa = 3.95

Molecular weight: 48.55 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4

0

4

1471

133

589

367.8

41.1

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.022 ± 0.754

1.564 ± 0.628

6.662 ± 0.395

4.215 ± 0.247

4.827 ± 0.793

6.662 ± 0.264

1.564 ± 0.525

5.303 ± 0.653

4.487 ± 0.387

9.925 ± 0.854

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

0.952 ± 0.116

4.895 ± 0.388

5.642 ± 0.701

2.855 ± 0.629

7.206 ± 0.726

7.478 ± 0.975

5.778 ± 0.661

6.526 ± 0.721

1.496 ± 0.319

3.943 ± 0.163
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski