Mimosa yellow leaf curl virus

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Repensiviricetes; Geplafuvirales; Geminiviridae; Begomovirus

Average proteome isoelectric point is 8.0

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A5H172|A5H172_9GEMI AC4 protein OS=Mimosa yellow leaf curl virus OX=390440 GN=AC4 PE=3 SV=1
MM1 pKa = 7.71APPKK5 pKa = 10.14RR6 pKa = 11.84FRR8 pKa = 11.84ISAKK12 pKa = 10.44NYY14 pKa = 8.46FLTYY18 pKa = 8.4PQCSLTKK25 pKa = 10.58EE26 pKa = 4.02EE27 pKa = 5.64ALSQLQNIQTPTNKK41 pKa = 10.0KK42 pKa = 8.87YY43 pKa = 10.85IKK45 pKa = 9.66ICRR48 pKa = 11.84EE49 pKa = 3.74LHH51 pKa = 6.25EE52 pKa = 5.87DD53 pKa = 4.22GSPHH57 pKa = 6.56LHH59 pKa = 6.24VLLQFEE65 pKa = 4.99GKK67 pKa = 9.65FVCTNNRR74 pKa = 11.84FFDD77 pKa = 4.02LVSPTRR83 pKa = 11.84SAHH86 pKa = 4.86FHH88 pKa = 6.55PNIQGAKK95 pKa = 9.25SSSDD99 pKa = 3.11VKK101 pKa = 11.24SYY103 pKa = 10.79IDD105 pKa = 3.8KK106 pKa = 11.28DD107 pKa = 3.26GDD109 pKa = 3.84TIEE112 pKa = 4.54WGDD115 pKa = 3.55FQIDD119 pKa = 3.22GRR121 pKa = 11.84SARR124 pKa = 11.84GGCQNANDD132 pKa = 4.04ACAEE136 pKa = 4.06ALNAATKK143 pKa = 9.8EE144 pKa = 3.69AALNIIRR151 pKa = 11.84EE152 pKa = 4.43KK153 pKa = 10.9LPKK156 pKa = 10.13DD157 pKa = 3.78YY158 pKa = 10.83IFQFHH163 pKa = 6.74NLNANLDD170 pKa = 4.25RR171 pKa = 11.84IFAPPLEE178 pKa = 4.42VFVCPFLSSSFDD190 pKa = 3.44QVPEE194 pKa = 3.87EE195 pKa = 4.25LEE197 pKa = 4.08VWVSEE202 pKa = 4.19NVRR205 pKa = 11.84DD206 pKa = 3.6AAARR210 pKa = 11.84PWRR213 pKa = 11.84PQSIVIEE220 pKa = 4.44GDD222 pKa = 3.12SRR224 pKa = 11.84TGKK227 pKa = 8.52TMWARR232 pKa = 11.84SLGPHH237 pKa = 6.71NYY239 pKa = 10.05LCGHH243 pKa = 7.46LGLSPKK249 pKa = 10.19VYY251 pKa = 11.0SNDD254 pKa = 2.25AWYY257 pKa = 10.92NVIDD261 pKa = 5.2DD262 pKa = 4.41VDD264 pKa = 3.61PHH266 pKa = 5.85YY267 pKa = 11.0LKK269 pKa = 10.7HH270 pKa = 6.2FKK272 pKa = 10.7EE273 pKa = 4.46FMGAQRR279 pKa = 11.84DD280 pKa = 3.81WQSNTKK286 pKa = 9.26YY287 pKa = 10.63GKK289 pKa = 9.49PIQIKK294 pKa = 10.42GGIPTIFLCNPGPTSSYY311 pKa = 11.03KK312 pKa = 10.55EE313 pKa = 3.94YY314 pKa = 10.94LDD316 pKa = 3.87EE317 pKa = 4.82EE318 pKa = 4.69KK319 pKa = 11.15NSALKK324 pKa = 10.55DD325 pKa = 3.36WALKK329 pKa = 9.35NAEE332 pKa = 4.86FITLTEE338 pKa = 4.09PLYY341 pKa = 11.19SGTNQSPAQHH351 pKa = 5.87SQEE354 pKa = 4.22EE355 pKa = 4.56ANSEE359 pKa = 4.01ASSS362 pKa = 3.33

Molecular weight: 40.91 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A5H174|A5H174_9GEMI Capsid protein OS=Mimosa yellow leaf curl virus OX=390440 GN=AV1 PE=3 SV=1
MM1 pKa = 7.71SKK3 pKa = 10.35RR4 pKa = 11.84PADD7 pKa = 3.52IVISTPASKK16 pKa = 10.31VRR18 pKa = 11.84RR19 pKa = 11.84RR20 pKa = 11.84SNFDD24 pKa = 2.76SPYY27 pKa = 10.03ASRR30 pKa = 11.84AAAPTVLVTNKK41 pKa = 9.33RR42 pKa = 11.84RR43 pKa = 11.84SWVNRR48 pKa = 11.84PMYY51 pKa = 9.89RR52 pKa = 11.84KK53 pKa = 8.44PRR55 pKa = 11.84MYY57 pKa = 10.84RR58 pKa = 11.84MYY60 pKa = 10.6KK61 pKa = 10.21SPDD64 pKa = 3.18VPRR67 pKa = 11.84GCEE70 pKa = 4.34GPCKK74 pKa = 9.32VQSYY78 pKa = 6.95EE79 pKa = 3.75QRR81 pKa = 11.84HH82 pKa = 5.6DD83 pKa = 3.46VAHH86 pKa = 5.81VGKK89 pKa = 9.73VICVSDD95 pKa = 3.47VTRR98 pKa = 11.84GNGLTHH104 pKa = 6.89RR105 pKa = 11.84VGKK108 pKa = 9.73RR109 pKa = 11.84FCVKK113 pKa = 10.32SIYY116 pKa = 10.42VLGKK120 pKa = 9.13IWMDD124 pKa = 3.35EE125 pKa = 4.04NIKK128 pKa = 9.32TKK130 pKa = 10.61NHH132 pKa = 6.03TNTVMFFLVRR142 pKa = 11.84DD143 pKa = 3.79RR144 pKa = 11.84RR145 pKa = 11.84PFGTPQDD152 pKa = 3.81FGQVFNMYY160 pKa = 10.66DD161 pKa = 3.86NEE163 pKa = 4.34PSTATVKK170 pKa = 10.73NDD172 pKa = 2.82NRR174 pKa = 11.84DD175 pKa = 3.13RR176 pKa = 11.84FQVLRR181 pKa = 11.84RR182 pKa = 11.84FQATVTGGQYY192 pKa = 10.83ASKK195 pKa = 9.78EE196 pKa = 3.78QAIVRR201 pKa = 11.84KK202 pKa = 9.26FMKK205 pKa = 10.33VNNHH209 pKa = 3.97VTYY212 pKa = 10.83NHH214 pKa = 6.01QEE216 pKa = 3.45AAKK219 pKa = 10.14YY220 pKa = 10.5DD221 pKa = 3.75NHH223 pKa = 6.5TEE225 pKa = 4.02NALLLYY231 pKa = 7.29MACTHH236 pKa = 7.07ASNPVYY242 pKa = 9.86ATLKK246 pKa = 9.47IRR248 pKa = 11.84IYY250 pKa = 10.65FYY252 pKa = 11.33DD253 pKa = 3.43SVQNN257 pKa = 3.87

Molecular weight: 29.77 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6

0

6

1089

85

362

181.5

20.79

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.061 ± 0.783

2.571 ± 0.437

4.867 ± 0.511

4.775 ± 0.822

4.224 ± 0.547

4.5 ± 0.449

3.857 ± 0.59

5.51 ± 0.709

5.326 ± 0.912

6.795 ± 0.938

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.928 ± 0.493

5.969 ± 0.83

6.061 ± 0.816

5.234 ± 0.564

7.071 ± 1.167

8.173 ± 1.641

5.693 ± 0.841

6.061 ± 1.291

1.469 ± 0.322

3.857 ± 0.493
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski