Amino acid dipepetide frequency for Streptomyces sp. ICC1

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	23.325AlaAla: 23.325 ± 0.182	1.148AlaCys: 1.148 ± 0.024	8.443AlaAsp: 8.443 ± 0.061	8.842AlaGlu: 8.842 ± 0.1	3.621AlaPhe: 3.621 ± 0.048	14.421AlaGly: 14.421 ± 0.098	2.931AlaHis: 2.931 ± 0.039	3.481AlaIle: 3.481 ± 0.044	3.105AlaLys: 3.105 ± 0.049	14.855AlaLeu: 14.855 ± 0.105	2.629AlaMet: 2.629 ± 0.035	2.008AlaAsn: 2.008 ± 0.037	7.744AlaPro: 7.744 ± 0.078	3.83AlaGln: 3.83 ± 0.043	10.402AlaArg: 10.402 ± 0.081	6.089AlaSer: 6.089 ± 0.06	6.928AlaThr: 6.928 ± 0.057	12.337AlaVal: 12.337 ± 0.08	1.915AlaTrp: 1.915 ± 0.029	2.751AlaTyr: 2.751 ± 0.036	0.0AlaXaa: 0.0 ± 0.0
Cys	1.161CysAla: 1.161 ± 0.027	0.092CysCys: 0.092 ± 0.007	0.453CysAsp: 0.453 ± 0.013	0.421CysGlu: 0.421 ± 0.015	0.228CysPhe: 0.228 ± 0.01	1.05CysGly: 1.05 ± 0.021	0.201CysHis: 0.201 ± 0.009	0.175CysIle: 0.175 ± 0.008	0.116CysLys: 0.116 ± 0.006	0.77CysLeu: 0.77 ± 0.02	0.13CysMet: 0.13 ± 0.008	0.143CysAsn: 0.143 ± 0.008	0.509CysPro: 0.509 ± 0.019	0.169CysGln: 0.169 ± 0.008	0.598CysArg: 0.598 ± 0.016	0.442CysSer: 0.442 ± 0.014	0.535CysThr: 0.535 ± 0.015	0.671CysVal: 0.671 ± 0.016	0.143CysTrp: 0.143 ± 0.007	0.149CysTyr: 0.149 ± 0.008	0.0CysXaa: 0.0 ± 0.0
Asp	7.275AspAla: 7.275 ± 0.055	0.44AspCys: 0.44 ± 0.015	2.965AspAsp: 2.965 ± 0.036	3.506AspGlu: 3.506 ± 0.04	1.591AspPhe: 1.591 ± 0.028	6.244AspGly: 6.244 ± 0.053	1.389AspHis: 1.389 ± 0.024	1.798AspIle: 1.798 ± 0.029	1.238AspLys: 1.238 ± 0.026	6.015AspLeu: 6.015 ± 0.048	0.784AspMet: 0.784 ± 0.019	0.919AspAsn: 0.919 ± 0.023	4.658AspPro: 4.658 ± 0.043	1.52AspGln: 1.52 ± 0.024	4.779AspArg: 4.779 ± 0.052	2.512AspSer: 2.512 ± 0.034	3.02AspThr: 3.02 ± 0.037	4.246AspVal: 4.246 ± 0.042	1.025AspTrp: 1.025 ± 0.024	1.024AspTyr: 1.024 ± 0.02	0.0AspXaa: 0.0 ± 0.0
Glu	7.589GluAla: 7.589 ± 0.092	0.364GluCys: 0.364 ± 0.012	2.794GluAsp: 2.794 ± 0.041	3.456GluGlu: 3.456 ± 0.054	1.505GluPhe: 1.505 ± 0.026	4.422GluGly: 4.422 ± 0.04	1.481GluHis: 1.481 ± 0.028	2.201GluIle: 2.201 ± 0.03	1.4GluLys: 1.4 ± 0.026	6.838GluLeu: 6.838 ± 0.064	0.864GluMet: 0.864 ± 0.021	1.039GluAsn: 1.039 ± 0.026	3.253GluPro: 3.253 ± 0.049	2.071GluGln: 2.071 ± 0.035	5.171GluArg: 5.171 ± 0.052	2.561GluSer: 2.561 ± 0.034	2.729GluThr: 2.729 ± 0.033	4.353GluVal: 4.353 ± 0.044	0.752GluTrp: 0.752 ± 0.017	1.103GluTyr: 1.103 ± 0.023	0.0GluXaa: 0.0 ± 0.0
Phe	3.832PheAla: 3.832 ± 0.044	0.26PheCys: 0.26 ± 0.009	1.83PheAsp: 1.83 ± 0.027	1.452PheGlu: 1.452 ± 0.025	0.867PhePhe: 0.867 ± 0.024	3.097PheGly: 3.097 ± 0.04	0.636PheHis: 0.636 ± 0.015	0.756PheIle: 0.756 ± 0.019	0.579PheLys: 0.579 ± 0.014	2.607PheLeu: 2.607 ± 0.033	0.405PheMet: 0.405 ± 0.016	0.583PheAsn: 0.583 ± 0.02	1.442PhePro: 1.442 ± 0.026	0.655PheGln: 0.655 ± 0.017	1.768PheArg: 1.768 ± 0.03	1.496PheSer: 1.496 ± 0.025	2.06PheThr: 2.06 ± 0.033	2.095PheVal: 2.095 ± 0.032	0.419PheTrp: 0.419 ± 0.014	0.578PheTyr: 0.578 ± 0.019	0.0PheXaa: 0.0 ± 0.0
Gly	11.978GlyAla: 11.978 ± 0.093	0.877GlyCys: 0.877 ± 0.019	4.995GlyAsp: 4.995 ± 0.044	5.111GlyGlu: 5.111 ± 0.045	2.999GlyPhe: 2.999 ± 0.036	9.301GlyGly: 9.301 ± 0.09	2.457GlyHis: 2.457 ± 0.035	3.616GlyIle: 3.616 ± 0.041	2.641GlyLys: 2.641 ± 0.042	9.647GlyLeu: 9.647 ± 0.066	1.987GlyMet: 1.987 ± 0.031	1.864GlyAsn: 1.864 ± 0.039	5.839GlyPro: 5.839 ± 0.064	2.79GlyGln: 2.79 ± 0.04	8.001GlyArg: 8.001 ± 0.067	5.851GlySer: 5.851 ± 0.065	6.241GlyThr: 6.241 ± 0.063	7.286GlyVal: 7.286 ± 0.058	1.792GlyTrp: 1.792 ± 0.029	2.305GlyTyr: 2.305 ± 0.031	0.0GlyXaa: 0.0 ± 0.0
His	2.711HisAla: 2.711 ± 0.036	0.204HisCys: 0.204 ± 0.009	1.248HisAsp: 1.248 ± 0.023	1.194HisGlu: 1.194 ± 0.022	0.633HisPhe: 0.633 ± 0.015	2.418HisGly: 2.418 ± 0.036	0.707HisHis: 0.707 ± 0.022	0.652HisIle: 0.652 ± 0.019	0.373HisLys: 0.373 ± 0.013	2.396HisLeu: 2.396 ± 0.035	0.356HisMet: 0.356 ± 0.012	0.354HisAsn: 0.354 ± 0.014	1.837HisPro: 1.837 ± 0.031	0.65HisGln: 0.65 ± 0.017	2.046HisArg: 2.046 ± 0.032	1.002HisSer: 1.002 ± 0.022	1.353HisThr: 1.353 ± 0.027	1.656HisVal: 1.656 ± 0.026	0.399HisTrp: 0.399 ± 0.014	0.449HisTyr: 0.449 ± 0.013	0.0HisXaa: 0.0 ± 0.0
Ile	4.919IleAla: 4.919 ± 0.048	0.302IleCys: 0.302 ± 0.011	2.141IleAsp: 2.141 ± 0.027	1.993IleGlu: 1.993 ± 0.034	0.705IlePhe: 0.705 ± 0.018	3.733IleGly: 3.733 ± 0.044	0.591IleHis: 0.591 ± 0.014	0.86IleIle: 0.86 ± 0.02	0.757IleLys: 0.757 ± 0.021	2.439IleLeu: 2.439 ± 0.03	0.453IleMet: 0.453 ± 0.013	0.704IleAsn: 0.704 ± 0.02	1.834IlePro: 1.834 ± 0.027	0.758IleGln: 0.758 ± 0.016	2.273IleArg: 2.273 ± 0.028	1.721IleSer: 1.721 ± 0.029	2.241IleThr: 2.241 ± 0.034	2.628IleVal: 2.628 ± 0.033	0.372IleTrp: 0.372 ± 0.013	0.487IleTyr: 0.487 ± 0.014	0.0IleXaa: 0.0 ± 0.0
Lys	3.157LysAla: 3.157 ± 0.051	0.132LysCys: 0.132 ± 0.008	1.403LysAsp: 1.403 ± 0.031	1.195LysGlu: 1.195 ± 0.026	0.479LysPhe: 0.479 ± 0.015	1.866LysGly: 1.866 ± 0.038	0.446LysHis: 0.446 ± 0.013	0.946LysIle: 0.946 ± 0.021	0.829LysLys: 0.829 ± 0.022	2.116LysLeu: 2.116 ± 0.03	0.424LysMet: 0.424 ± 0.013	0.534LysAsn: 0.534 ± 0.02	1.432LysPro: 1.432 ± 0.028	0.687LysGln: 0.687 ± 0.021	1.428LysArg: 1.428 ± 0.025	1.151LysSer: 1.151 ± 0.025	1.397LysThr: 1.397 ± 0.029	1.945LysVal: 1.945 ± 0.033	0.282LysTrp: 0.282 ± 0.011	0.459LysTyr: 0.459 ± 0.017	0.0LysXaa: 0.0 ± 0.0
Leu	15.797LeuAla: 15.797 ± 0.111	0.865LeuCys: 0.865 ± 0.022	6.374LeuAsp: 6.374 ± 0.058	4.721LeuGlu: 4.721 ± 0.046	2.634LeuPhe: 2.634 ± 0.035	9.501LeuGly: 9.501 ± 0.067	2.233LeuHis: 2.233 ± 0.033	3.346LeuIle: 3.346 ± 0.044	2.023LeuLys: 2.023 ± 0.034	11.451LeuLeu: 11.451 ± 0.098	1.635LeuMet: 1.635 ± 0.029	1.7LeuAsn: 1.7 ± 0.032	6.572LeuPro: 6.572 ± 0.069	2.129LeuGln: 2.129 ± 0.031	8.514LeuArg: 8.514 ± 0.074	5.262LeuSer: 5.262 ± 0.044	6.677LeuThr: 6.677 ± 0.058	8.688LeuVal: 8.688 ± 0.067	1.32LeuTrp: 1.32 ± 0.027	1.832LeuTyr: 1.832 ± 0.027	0.0LeuXaa: 0.0 ± 0.0
Met	2.347MetAla: 2.347 ± 0.031	0.143MetCys: 0.143 ± 0.008	0.903MetAsp: 0.903 ± 0.019	0.747MetGlu: 0.747 ± 0.015	0.449MetPhe: 0.449 ± 0.015	1.353MetGly: 1.353 ± 0.025	0.319MetHis: 0.319 ± 0.012	0.679MetIle: 0.679 ± 0.019	0.447MetLys: 0.447 ± 0.013	1.701MetLeu: 1.701 ± 0.025	0.304MetMet: 0.304 ± 0.013	0.469MetAsn: 0.469 ± 0.013	1.178MetPro: 1.178 ± 0.021	0.422MetGln: 0.422 ± 0.013	1.472MetArg: 1.472 ± 0.023	1.31MetSer: 1.31 ± 0.022	1.519MetThr: 1.519 ± 0.024	1.281MetVal: 1.281 ± 0.024	0.239MetTrp: 0.239 ± 0.01	0.329MetTyr: 0.329 ± 0.011	0.0MetXaa: 0.0 ± 0.0
Asn	2.219AsnAla: 2.219 ± 0.04	0.172AsnCys: 0.172 ± 0.01	0.902AsnAsp: 0.902 ± 0.022	0.845AsnGlu: 0.845 ± 0.021	0.516AsnPhe: 0.516 ± 0.015	1.913AsnGly: 1.913 ± 0.037	0.4AsnHis: 0.4 ± 0.013	0.646AsnIle: 0.646 ± 0.02	0.41AsnLys: 0.41 ± 0.015	1.726AsnLeu: 1.726 ± 0.031	0.309AsnMet: 0.309 ± 0.011	0.449AsnAsn: 0.449 ± 0.018	1.413AsnPro: 1.413 ± 0.027	0.534AsnGln: 0.534 ± 0.015	1.275AsnArg: 1.275 ± 0.027	0.957AsnSer: 0.957 ± 0.023	1.168AsnThr: 1.168 ± 0.029	1.34AsnVal: 1.34 ± 0.026	0.304AsnTrp: 0.304 ± 0.013	0.373AsnTyr: 0.373 ± 0.013	0.0AsnXaa: 0.0 ± 0.0
Pro	9.754ProAla: 9.754 ± 0.1	0.379ProCys: 0.379 ± 0.015	4.343ProAsp: 4.343 ± 0.048	4.245ProGlu: 4.245 ± 0.043	1.567ProPhe: 1.567 ± 0.024	7.309ProGly: 7.309 ± 0.071	1.355ProHis: 1.355 ± 0.025	1.271ProIle: 1.271 ± 0.027	1.241ProLys: 1.241 ± 0.027	5.405ProLeu: 5.405 ± 0.058	1.037ProMet: 1.037 ± 0.024	0.933ProAsn: 0.933 ± 0.023	3.513ProPro: 3.513 ± 0.052	1.795ProGln: 1.795 ± 0.035	3.99ProArg: 3.99 ± 0.051	3.134ProSer: 3.134 ± 0.041	3.015ProThr: 3.015 ± 0.036	5.685ProVal: 5.685 ± 0.055	0.923ProTrp: 0.923 ± 0.022	1.425ProTyr: 1.425 ± 0.028	0.0ProXaa: 0.0 ± 0.0
Gln	3.774GlnAla: 3.774 ± 0.043	0.19GlnCys: 0.19 ± 0.009	1.367GlnAsp: 1.367 ± 0.025	1.414GlnGlu: 1.414 ± 0.027	0.666GlnPhe: 0.666 ± 0.018	2.345GlnGly: 2.345 ± 0.033	0.646GlnHis: 0.646 ± 0.016	1.097GlnIle: 1.097 ± 0.023	0.608GlnLys: 0.608 ± 0.016	2.933GlnLeu: 2.933 ± 0.037	0.479GlnMet: 0.479 ± 0.015	0.506GlnAsn: 0.506 ± 0.017	1.666GlnPro: 1.666 ± 0.03	1.162GlnGln: 1.162 ± 0.035	2.207GlnArg: 2.207 ± 0.031	1.316GlnSer: 1.316 ± 0.026	1.371GlnThr: 1.371 ± 0.028	2.28GlnVal: 2.28 ± 0.033	0.479GlnTrp: 0.479 ± 0.015	0.624GlnTyr: 0.624 ± 0.016	0.0GlnXaa: 0.0 ± 0.0
Arg	9.98ArgAla: 9.98 ± 0.09	0.601ArgCys: 0.601 ± 0.015	4.003ArgAsp: 4.003 ± 0.047	4.723ArgGlu: 4.723 ± 0.051	2.276ArgPhe: 2.276 ± 0.032	5.966ArgGly: 5.966 ± 0.058	2.023ArgHis: 2.023 ± 0.034	3.285ArgIle: 3.285 ± 0.036	1.652ArgLys: 1.652 ± 0.027	8.579ArgLeu: 8.579 ± 0.081	1.755ArgMet: 1.755 ± 0.027	1.267ArgAsn: 1.267 ± 0.025	5.02ArgPro: 5.02 ± 0.071	2.142ArgGln: 2.142 ± 0.03	7.391ArgArg: 7.391 ± 0.078	4.078ArgSer: 4.078 ± 0.046	5.394ArgThr: 5.394 ± 0.052	5.579ArgVal: 5.579 ± 0.056	1.369ArgTrp: 1.369 ± 0.027	1.697ArgTyr: 1.697 ± 0.026	0.0ArgXaa: 0.0 ± 0.0
Ser	7.116SerAla: 7.116 ± 0.07	0.449SerCys: 0.449 ± 0.014	2.575SerAsp: 2.575 ± 0.035	2.424SerGlu: 2.424 ± 0.036	1.545SerPhe: 1.545 ± 0.03	6.073SerGly: 6.073 ± 0.062	1.025SerHis: 1.025 ± 0.021	1.473SerIle: 1.473 ± 0.026	1.066SerLys: 1.066 ± 0.026	4.865SerLeu: 4.865 ± 0.045	1.096SerMet: 1.096 ± 0.018	0.89SerAsn: 0.89 ± 0.021	3.278SerPro: 3.278 ± 0.048	1.271SerGln: 1.271 ± 0.024	3.581SerArg: 3.581 ± 0.04	2.821SerSer: 2.821 ± 0.043	3.101SerThr: 3.101 ± 0.041	4.326SerVal: 4.326 ± 0.042	0.918SerTrp: 0.918 ± 0.021	1.259SerTyr: 1.259 ± 0.03	0.0SerXaa: 0.0 ± 0.0
Thr	9.053ThrAla: 9.053 ± 0.07	0.442ThrCys: 0.442 ± 0.013	3.39ThrAsp: 3.39 ± 0.043	3.075ThrGlu: 3.075 ± 0.038	1.544ThrPhe: 1.544 ± 0.028	6.931ThrGly: 6.931 ± 0.066	1.163ThrHis: 1.163 ± 0.025	1.672ThrIle: 1.672 ± 0.03	1.2ThrLys: 1.2 ± 0.028	5.61ThrLeu: 5.61 ± 0.053	0.939ThrMet: 0.939 ± 0.02	1.002ThrAsn: 1.002 ± 0.023	4.07ThrPro: 4.07 ± 0.043	1.309ThrGln: 1.309 ± 0.025	3.812ThrArg: 3.812 ± 0.043	3.08ThrSer: 3.08 ± 0.045	3.764ThrThr: 3.764 ± 0.063	5.783ThrVal: 5.783 ± 0.059	0.887ThrTrp: 0.887 ± 0.019	1.314ThrTyr: 1.314 ± 0.038	0.0ThrXaa: 0.0 ± 0.0
Val	10.304ValAla: 10.304 ± 0.071	0.76ValCys: 0.76 ± 0.02	4.628ValAsp: 4.628 ± 0.045	4.507ValGlu: 4.507 ± 0.049	2.447ValPhe: 2.447 ± 0.036	6.258ValGly: 6.258 ± 0.061	1.893ValHis: 1.893 ± 0.027	2.893ValIle: 2.893 ± 0.036	1.834ValLys: 1.834 ± 0.032	9.505ValLeu: 9.505 ± 0.071	1.467ValMet: 1.467 ± 0.027	1.69ValAsn: 1.69 ± 0.032	5.177ValPro: 5.177 ± 0.057	2.007ValGln: 2.007 ± 0.028	7.018ValArg: 7.018 ± 0.066	4.416ValSer: 4.416 ± 0.041	5.252ValThr: 5.252 ± 0.05	7.443ValVal: 7.443 ± 0.07	1.167ValTrp: 1.167 ± 0.022	1.546ValTyr: 1.546 ± 0.025	0.0ValXaa: 0.0 ± 0.0
Trp	1.71TrpAla: 1.71 ± 0.03	0.163TrpCys: 0.163 ± 0.008	0.81TrpAsp: 0.81 ± 0.02	0.755TrpGlu: 0.755 ± 0.018	0.511TrpPhe: 0.511 ± 0.015	1.044TrpGly: 1.044 ± 0.023	0.384TrpHis: 0.384 ± 0.012	0.603TrpIle: 0.603 ± 0.017	0.375TrpLys: 0.375 ± 0.014	1.772TrpLeu: 1.772 ± 0.033	0.297TrpMet: 0.297 ± 0.013	0.435TrpAsn: 0.435 ± 0.016	0.829TrpPro: 0.829 ± 0.019	0.639TrpGln: 0.639 ± 0.017	1.3TrpArg: 1.3 ± 0.024	1.004TrpSer: 1.004 ± 0.021	1.09TrpThr: 1.09 ± 0.024	0.961TrpVal: 0.961 ± 0.021	0.346TrpTrp: 0.346 ± 0.013	0.37TrpTyr: 0.37 ± 0.013	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.802TyrAla: 2.802 ± 0.04	0.173TyrCys: 0.173 ± 0.008	1.382TyrAsp: 1.382 ± 0.031	1.319TyrGlu: 1.319 ± 0.024	0.636TyrPhe: 0.636 ± 0.018	2.332TyrGly: 2.332 ± 0.032	0.358TyrHis: 0.358 ± 0.013	0.462TyrIle: 0.462 ± 0.014	0.413TyrLys: 0.413 ± 0.015	2.043TyrLeu: 2.043 ± 0.029	0.26TyrMet: 0.26 ± 0.01	0.383TyrAsn: 0.383 ± 0.015	1.056TyrPro: 1.056 ± 0.023	0.59TyrGln: 0.59 ± 0.016	1.756TyrArg: 1.756 ± 0.031	0.947TyrSer: 0.947 ± 0.022	1.184TyrThr: 1.184 ± 0.026	1.631TyrVal: 1.631 ± 0.026	0.346TyrTrp: 0.346 ± 0.012	0.433TyrTyr: 0.433 ± 0.014	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 7671 proteins (2438377 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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