Clostridium sp. CT4

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae; Clostridium; unclassified Clostridium

Average proteome isoelectric point is 6.5

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5184 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3R5UCY7|A0A3R5UCY7_9CLOT ABC transporter ATP-binding protein OS=Clostridium sp. CT4 OX=2320868 GN=C1I91_00475 PE=4 SV=1
MM1 pKa = 7.59SDD3 pKa = 3.09KK4 pKa = 10.91NKK6 pKa = 10.33PYY8 pKa = 10.84LPDD11 pKa = 3.31EE12 pKa = 4.62RR13 pKa = 11.84DD14 pKa = 3.03WYY16 pKa = 11.11SPEE19 pKa = 3.69GHH21 pKa = 7.08DD22 pKa = 5.76LISLSSSGNTYY33 pKa = 9.84TKK35 pKa = 10.71LPNPKK40 pKa = 8.81TDD42 pKa = 4.53PIVVNNISYY51 pKa = 9.57MEE53 pKa = 4.35FDD55 pKa = 3.74YY56 pKa = 11.19TDD58 pKa = 3.0QSLYY62 pKa = 11.05EE63 pKa = 5.56DD64 pKa = 5.76DD65 pKa = 5.18DD66 pKa = 4.29TDD68 pKa = 4.99DD69 pKa = 3.71EE70 pKa = 4.47QQ71 pKa = 5.82

Molecular weight: 8.25 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3R5TGR4|A0A3R5TGR4_9CLOT Amino acid ABC transporter permease OS=Clostridium sp. CT4 OX=2320868 GN=C1I91_16405 PE=3 SV=1
MM1 pKa = 7.67SRR3 pKa = 11.84DD4 pKa = 3.6NNNGGMKK11 pKa = 10.39RR12 pKa = 11.84NGGKK16 pKa = 8.04MRR18 pKa = 11.84RR19 pKa = 11.84SKK21 pKa = 10.9RR22 pKa = 11.84KK23 pKa = 7.93VCAFCVEE30 pKa = 4.24KK31 pKa = 10.25ATSIDD36 pKa = 3.48YY37 pKa = 11.07KK38 pKa = 10.97DD39 pKa = 3.22INKK42 pKa = 9.06LRR44 pKa = 11.84KK45 pKa = 9.21YY46 pKa = 7.83VTEE49 pKa = 4.22RR50 pKa = 11.84GKK52 pKa = 10.15ILPRR56 pKa = 11.84RR57 pKa = 11.84ISGTCAKK64 pKa = 9.97HH65 pKa = 5.6QRR67 pKa = 11.84QLTDD71 pKa = 4.2AIKK74 pKa = 10.26RR75 pKa = 11.84SRR77 pKa = 11.84NIALLPFTTEE87 pKa = 3.59

Molecular weight: 10.03 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5184

0

5184

1615987

25

2855

311.7

35.19

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.866 ± 0.034

1.069 ± 0.013

5.689 ± 0.031

7.094 ± 0.044

4.494 ± 0.026

6.263 ± 0.035

1.385 ± 0.014

9.394 ± 0.041

8.673 ± 0.036

9.244 ± 0.037

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.508 ± 0.017

6.087 ± 0.036

2.763 ± 0.017

2.495 ± 0.018

3.488 ± 0.024

6.796 ± 0.032

5.044 ± 0.029

6.572 ± 0.026

0.779 ± 0.012

4.296 ± 0.024
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski