Aspergillus caelatus
Average proteome isoelectric point is 6.41
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 13914 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A5N7AB24|A0A5N7AB24_9EURO Endo-1 4-beta-xylanase OS=Aspergillus caelatus OX=61420 GN=BDV27DRAFT_143531 PE=3 SV=1
MM1 pKa = 7.64 PFSAAIASAILGASFALAAPPVDD24 pKa = 3.55 IGDD27 pKa = 3.09 IDD29 pKa = 4.57 EE30 pKa = 4.43 YY31 pKa = 11.82 VKK33 pKa = 10.38 VTDD36 pKa = 5.18 LEE38 pKa = 4.0 ITRR41 pKa = 11.84 AAFGKK46 pKa = 8.67 VTDD49 pKa = 3.93 VSFKK53 pKa = 11.11 LSGDD57 pKa = 3.5 DD58 pKa = 3.86 ATNLACSAKK67 pKa = 10.29 NPTLGYY73 pKa = 9.99 EE74 pKa = 4.35 VADD77 pKa = 3.71 STRR80 pKa = 11.84 CGDD83 pKa = 3.22 SDD85 pKa = 4.3 YY86 pKa = 11.36 LFNLLRR92 pKa = 11.84 GEE94 pKa = 4.21 DD95 pKa = 3.14 TGYY98 pKa = 11.45 ALAVFHH104 pKa = 6.82 EE105 pKa = 4.62 FQVDD109 pKa = 3.61 GSGTYY114 pKa = 9.98 SAGQYY119 pKa = 9.93 GQDD122 pKa = 3.49 EE123 pKa = 4.53 FLVDD127 pKa = 4.78 CEE129 pKa = 4.75 TNDD132 pKa = 4.6 DD133 pKa = 3.93 VQNCKK138 pKa = 8.52 STQSPITIILTGQQ151 pKa = 2.96
Molecular weight: 16.0 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.709
IPC2_protein 3.846
IPC_protein 3.834
Toseland 3.605
ProMoST 4.012
Dawson 3.834
Bjellqvist 3.986
Wikipedia 3.795
Rodwell 3.656
Grimsley 3.516
Solomon 3.821
Lehninger 3.783
Nozaki 3.948
DTASelect 4.215
Thurlkill 3.668
EMBOSS 3.795
Sillero 3.961
Patrickios 0.998
IPC_peptide 3.821
IPC2_peptide 3.923
IPC2.peptide.svr19 3.839
Protein with the highest isoelectric point:
>tr|A0A5N6ZXY1|A0A5N6ZXY1_9EURO Uncharacterized protein OS=Aspergillus caelatus OX=61420 GN=BDV27DRAFT_166341 PE=4 SV=1
MM1 pKa = 7.88 PSHH4 pKa = 6.91 KK5 pKa = 10.39 SFRR8 pKa = 11.84 TKK10 pKa = 10.45 QKK12 pKa = 9.54 LAKK15 pKa = 9.74 AQRR18 pKa = 11.84 QNRR21 pKa = 11.84 PIPQWIRR28 pKa = 11.84 LRR30 pKa = 11.84 TGNTIRR36 pKa = 11.84 YY37 pKa = 5.79 NAKK40 pKa = 8.89 RR41 pKa = 11.84 RR42 pKa = 11.84 HH43 pKa = 4.14 WRR45 pKa = 11.84 KK46 pKa = 7.51 TRR48 pKa = 11.84 LGLL51 pKa = 4.09
Molecular weight: 6.28 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.413
IPC2_protein 11.096
IPC_protein 12.457
Toseland 12.618
ProMoST 13.1
Dawson 12.618
Bjellqvist 12.603
Wikipedia 13.086
Rodwell 12.34
Grimsley 12.661
Solomon 13.1
Lehninger 13.013
Nozaki 12.618
DTASelect 12.603
Thurlkill 12.618
EMBOSS 13.115
Sillero 12.618
Patrickios 12.076
IPC_peptide 13.115
IPC2_peptide 12.091
IPC2.peptide.svr19 9.034
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
13914
0
13914
6418083
49
7733
461.3
51.19
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.195 ± 0.017
1.381 ± 0.01
5.541 ± 0.014
5.983 ± 0.02
3.888 ± 0.011
6.829 ± 0.019
2.459 ± 0.009
5.222 ± 0.014
4.542 ± 0.018
9.244 ± 0.024
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.195 ± 0.008
3.744 ± 0.01
5.831 ± 0.023
3.996 ± 0.013
5.937 ± 0.018
8.234 ± 0.022
5.943 ± 0.012
6.291 ± 0.017
1.553 ± 0.008
2.992 ± 0.01
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here