Alkaliphilus oremlandii (strain OhILAs) (Clostridium oremlandii (strain OhILAs))

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae; Alkaliphilus; Alkaliphilus oremlandii

Average proteome isoelectric point is 6.24

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2828 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A8MI57|A8MI57_ALKOO Uracil-xanthine permease OS=Alkaliphilus oremlandii (strain OhILAs) OX=350688 GN=Clos_1951 PE=3 SV=1
MM1 pKa = 7.69KK2 pKa = 10.73SNFLLWNEE10 pKa = 4.16DD11 pKa = 2.85MDD13 pKa = 4.1RR14 pKa = 11.84VYY16 pKa = 11.35GKK18 pKa = 10.79VSDD21 pKa = 4.83FEE23 pKa = 4.31NQGDD27 pKa = 4.65FINTVKK33 pKa = 10.49QYY35 pKa = 11.04CKK37 pKa = 10.43DD38 pKa = 3.66VEE40 pKa = 4.28EE41 pKa = 4.87GDD43 pKa = 4.47CIVEE47 pKa = 4.31NIEE50 pKa = 4.22IDD52 pKa = 3.87TCVSTCNGIEE62 pKa = 3.91AEE64 pKa = 4.24TLIKK68 pKa = 10.57IKK70 pKa = 10.25DD71 pKa = 3.6TDD73 pKa = 3.62IEE75 pKa = 4.05IATYY79 pKa = 11.18YY80 pKa = 9.33MADD83 pKa = 3.21VCIDD87 pKa = 3.52DD88 pKa = 3.75

Molecular weight: 10.09 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A8MLK0|A8MLK0_ALKOO Uncharacterized protein OS=Alkaliphilus oremlandii (strain OhILAs) OX=350688 GN=Clos_0552 PE=4 SV=1
MM1 pKa = 7.42ARR3 pKa = 11.84VAYY6 pKa = 10.24YY7 pKa = 8.9GTGRR11 pKa = 11.84RR12 pKa = 11.84KK13 pKa = 9.83QSIARR18 pKa = 11.84VRR20 pKa = 11.84LVPGEE25 pKa = 4.04GNIKK29 pKa = 10.18INNRR33 pKa = 11.84DD34 pKa = 2.92IEE36 pKa = 4.53EE37 pKa = 4.14YY38 pKa = 10.46FKK40 pKa = 11.76YY41 pKa = 8.99EE42 pKa = 3.75TLKK45 pKa = 10.68RR46 pKa = 11.84DD47 pKa = 2.94VRR49 pKa = 11.84MPLTITDD56 pKa = 3.68TLSRR60 pKa = 11.84FDD62 pKa = 4.26VIATVAGGGYY72 pKa = 8.23TGQAGALRR80 pKa = 11.84HH81 pKa = 5.88GISRR85 pKa = 11.84ALTKK89 pKa = 10.32SDD91 pKa = 3.28EE92 pKa = 4.32EE93 pKa = 4.38LRR95 pKa = 11.84GTLKK99 pKa = 10.75RR100 pKa = 11.84AGFLTRR106 pKa = 11.84DD107 pKa = 3.14ARR109 pKa = 11.84MKK111 pKa = 10.04EE112 pKa = 3.81RR113 pKa = 11.84KK114 pKa = 9.65KK115 pKa = 11.07YY116 pKa = 9.44GLKK119 pKa = 10.05AARR122 pKa = 11.84RR123 pKa = 11.84SPQFSKK129 pKa = 11.0RR130 pKa = 3.44

Molecular weight: 14.75 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2828

0

2828

874563

37

2075

309.3

34.75

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.351 ± 0.057

0.942 ± 0.02

5.264 ± 0.034

7.82 ± 0.06

4.132 ± 0.035

6.904 ± 0.054

1.776 ± 0.018

9.521 ± 0.045

7.789 ± 0.046

9.291 ± 0.043

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.867 ± 0.022

5.206 ± 0.036

3.12 ± 0.026

2.927 ± 0.022

3.865 ± 0.027

5.955 ± 0.031

5.076 ± 0.032

6.753 ± 0.04

0.696 ± 0.013

3.744 ± 0.035
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski